A lipid-enriched strain of Botryococcus braunii (UTEX 572) was cultivated in a semi-batch aeration tank to enhance biomass as well as to develop intracellular lipids and fatty acids. A 30 day period of incubation produced 1.39 g/L of biomass and 0.31 g/L of total lipids in the biomass. The grown biomass was pre-treated using several methods to extract the total lipid content efficiently: ultrasonication was found to yield the highest percentage of lipids-namely 19.8% per biomass. Direct heating of biomass in an autoclave also showed better performance than when using only conventional solvent extraction. To enhance the biomass harvest and lipid extraction efficiency, coagulation and flocculation steps were added to the extraction process. It is noteworthy that not only the solvent type but also the solvent/biomass ratio greatly affected efficiency. In addition, the moisture content of the harvested(wet) biomass affected the efficiency significantly. This study elucidated the need for future research on optimizing this extraction process.

Abstract
1. 서 론
2. 재료 및 방법
    2.1. 미세조류의 배양과 사용배지
    2.2. 균체량 측정
    2.3. 전처리, 추출 및 지질측정
    2.4. 반회분식 배양(semi-batch cultivation)
3. 결과 및 고찰
    3.2. 부가공정을 통한 조류균체의 수확
    3.3 공정변수에 따른 지질 추출 최적화
4. 결 론
REFERENCES

• 권성현(경상대학교 해양과학대학 해양환경공학과) | Sung-Hyun Kwon (Department of Marine Environmental Engineering, Gyeongsang National University)
• 조대철(순천향대학교 에너지환경공학과) | Daechul Cho (Department of Energy & Environmental Engineering, Soonchunhyang University) Corresponding author