산업식품공학 제23권 제4호 (p.278-282)

Real Time PCR을 활용한 가공식품에서의 밀 검출에 관한 연구

Detection of Wheat in Processed Food Using Real Time PCR
키워드 :
wheat,ITS 2,real-time PCR

목차

Abstract
서 론
재료 및 방법
   재료 및 분석기기
   유전자추출
   염기서열 분석 및 프라이머 제작
   유전자 증폭
   밀 유전자 표준품
결과 및 고찰
요 약
References

초록

This study constructed a wheat-specific primer and a probe using the internal transcribed spacer (ITS). 2 regions of wheat (Triticum aestivum) and real-time PCR conditions were established. The calibration curve showed a slope of -3.356, a correlation coefficient of 0.998, and an amplification efficiency of 98.589%. Experiments were carried out on the rice flour mixed with 50%, 10%, 1%, 0.1%, 0.01%, and 0.001% of wheat. The result showed that it was possible to detect samples mixed with up to 0.01% of wheat. As a result of checking, the wheat detection potential of rice, 34 processed foods, and seven processed foods was ascertained. The real-time PCR method using the wheatspecific primer and probe developed in this study can be used to identify the authenticity of the raw materials, such as the incorrect indication of the raw materials utilized and the unintended mixing of wheat during the manufacturing process.