한국버섯학회지 제17권 제4호 (p.275-283)

|Research Article|
해조류 기반 엘리시터 처리에 의한 꽃송이버섯의 GABA 함량 증가 및 흥분성 신경세포의 수상돌기 발달 억제

Enhanced GABA content from sodium alginate-induced Sparassis latifolia influences dendrite development in primary cortical neurons
키워드 :
Sparassis latifolia,Elicitor,GABA,β-Glucan,Primary cortical neurons

목차

ABSTRACT
서 론
재료 및 방법
   꽃송이버섯 공시균주 및 생장
   해조류 기반 엘리시터 제조
   β-Glucan 측정
   GABA 측정
   초대 신경세포 배양
   면역형광법
   MTT assay
   Western blotting
   Dendrite outgrowth 측정
   통계
결과 및 고찰
   β-Glucan 측정결과
   GABA 측정결과
   독성실험결과
   초대 신경세포 수상돌기 발달 결과
   신경세포 내 신호전달경로의 변화결과
결 론
REFERENCES

초록

Sparassis latifolia is a fungus abundant in β-glucan and amino acids and is highly valued as a medicinal mushroom. Among amino acids, γ-aminobutyric acid (GABA) is a free amino acid and has biological effects, such as increase/decrease of hypertension, improvement of cerebral blood flow, and prevention of dementia. In this study, biological elicitors were used to increase bioactive substances as a biofortification method. Sodium alginate extracted from seaweed (Sargassum horneri, Sargassum fulvellum, Sargassum fusiforme) were used as the elicitor. The levels of β-glucan and GABA in the mycelium and fruiting body grown by adding the elicitor to the medium were investigated. Addition of sodium alginate positively affected GABA production and negatively affected the β-glucan production in these fungi. Sodium alginates extracted from S. fulvellum induced the highest increase in GABA in the mycelium and fruiting bodies. Moreover, we investigated the effects of the extracts from mycelium and fruiting bodies on dendrite development in primary cortical neurons. We found that the extract from the fruiting bodies of sodium alginate treated fungi with increased levels of GABA inhibited the dendrite outgrowth of excitatory neurons, but not inhibitory neurons.