한국식품영양학회지 Vol. 32 No. 6 (p.581-588)

|연구논문|
나문재(Suaeda glauca (Bunge) Bunge) 추출물과 분획물의 효능에 관한 연구

A Study of Effects of Suaeda glauca (Bunge) Bunge Extract and Its Fractions
키워드 :
S. glauca,Bunge,Bunge,total polyphenol contents,electron donating ability,superoxide anion radical

목차

Abstract
서 론
재료 및 방법
   1. 실험재료, 시약 및 기구
   2. Suaeda glauca (Bunge) Bunge의 추출 및 분리
   3. 총 폴리페놀 함량 측정
   4. 전자공여능 측정
   5. Superoxide anion radical의 제거능
   6. Hydrogen peroxide 제거능 측정
   7. 자료의 통계처리
결과 및 고찰
   1. S. glauca (Bunge) Bunge 추출
   2. 총 폴리페놀 함량
   3. 전자공여능측정(Electron Donating Ability Measurement)
   4. Superoxide radical anion 제거능 측정
   5. Hydrogen peroxide 제거능 측정
요약 및 결론
References

초록

In this study, S. glauca (Bunge) Bunge extract was testified total polyphenol contents, electron donating ability on DPPH, and scavenging ability of superoxide anion radical and hydrogen peroxide. Total polyphenol contents of S. glauca (Bunge) Bunge extract were 7,053.30±915.93 μg GAE/mL. In the fractions, fraction by ethanol was the highest content of 10,973.30±1,000.24 μg GAE/mL. Fractions of ethyl acetate and water were 2,386.70±166.53 μg GAE/mL, 2,413.30±41.63 μg GAE/mL respectively. It was shown that total content of polyphenol according to solvent was significant relation at p<0.05. In the experiment of the electron donating ability, 70% ethanol extract and methylene chloride fraction were -246.15±24.17%, -254.01± 16.54% respectively. In case of ethyl acetate fraction, it was the highest electron donating ability to DPPH radical, 39.06±0.34%, and then water fraction of 36.71±5.55%, ethanol fraction of 29.77±2.57%, gradually. Electron donating abilities revealed significant difference (p<0.05) between the solvents. The superoxide radical scavenging ability of standard material was 0.029±0.0011; 70% ethanol extract of 0.022±0.00052, methylene chloride fraction of 0.027±0.00031, ethyl acetate fraction of 0.024±0.0011, ethanol fraction of 0.021±0.00024 and water fraction of 0.024±0.00019. Hydrogen peroxide scavenging ability of 70% ethanol extract was -0.0029±0.00040 and the others were as follow; methylene chloride fraction (-0.0042±0.00058), ethyl acetate fraction (-0.003± 0.0041), ethanol fraction (-0.0029±0.0015) and water fraction (-0.0028±0.00090).