한국식품영양학회지 Vol. 32 No. 6 (p.611-619)

|연구논문|
더덕 추출물과 용매 분획물의 항산화 및 티로시나아제 저해활성

Antioxidant Activity and Tyrosinase Inhibitory Activities of Codonopsis lanceolata Extract and Solvent Fraction
키워드 :
Codonopsis lanceolata,antioxidant activity,mushroom tyrosinase,tangshenoside I,lobetyolin

목차

Abstract
서 론
재료 및 방법
   1. 재료 및 시약
   2. 추출물 및 용매분획물 제조
   3. 총 폴리페놀 및 총 플라보노이드 함량
   4. DPPH 및 ABTS 라디칼 소거 활성
   5. Mushroom tyrosinase 저해 활성
   6. 더덕 EA 분획물 정성 분석
   7. Tangshenoside I과 lobetyolin 정량 분석
   8. 통계처리
결과 및 고찰
   1. 총 폴리페놀 및 총 플라보노이드 함량 분석
   2. DPPH 및 ABTS 라디칼 소거 활성
   3. Mushroom tyrosinase 저해 활성
   4. 더덕 EA 분획물 정성 분석
   5. Tangshenoside I과 lobetyolin 정량 분석 및 tyrosinase저해활성
요약 및 결론
REFERENCES

초록

The purpose of this study was to investigate the antioxidant activity and tyrosinase inhibitory activity of Codonopsis lanceolata 50% ethanol extract, and its solvent fractions (n-hexane, ethyl acetate (EA), n-butanol, water). The main components of the EA fraction were qualitatively analyzed using UPLC Q-ToF/MS. Additionally, a quantitative analysis was performed using UPLC. As a result, the total polyphenol content was 113.36 mg gallic acid/g in the EA fraction, which contained the largest amount of the C. lancolata solvent fractions. Also EA showed the highest antioxidant activity than other fractions. The IC50 of DPPH(2,2-diphenyl-1- picrylhydrazyl) radical scavenging activity was 0.03 mg/mL and the IC50 of ABTS [2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate)] radical scavenging activity was 0.049 mg/mL. The EA fraction showed tyrosinase inhibitory activity than other fractions and especially inhibited monophenolase oxidase reaction higher than diphenolase oxidase reaction. The monophenolase oxidase inhibited 55% when the concentration of the EA fraction was 0.25 mg/mL. As a result of Q-ToF/MS analysis, it was confirmed that tangshenoside I and lobetyolin were the main components of EA fraction. Thus, these results suggest that C. lanceolata may be used as a potent source of cosmetic agents.