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pp.636-642
The purpose of this study was to examine biological activities, including total contents of polyphenol, antioxidant activities, inhibitory activities of tyrosinase, and protective effect against oxidative stress in the HepG2 cells of ethanol extracts from wheat sprout. The antioxidant activity of extracts was determined by ABTS and DPPH radical scavenging activities. Ethanol extracts were tested using different ethanol concentrations (0%, 30%, 50%, 80% and 95%, respectively). The highest amount of total polyphenol was extracted by 50% and 80% ethanol which was 26.3 and 26.8 mg gallic acid equivalents/g sample, respectively. High levels of ABTS and DPPH radical scavenging activity were found in 50% ethanol (26.7 and 15.0 mg TEAC/g sample, respectively) and 80% (24.3 and 16.1 mg TEAC/g sample, respectively) ethanol extracts. Also, 50% and 80% ethanol extracts indicated higher inhibitory activities of tyrosinase compared with other extracts. In the cell-based assay, pre-treatment of the HepG2 cells with wheat sprout extracts prevented the cell damage induced by TBHP (tert-butyl hydroperoxide). The results of this study indicate that wheat sprout has significantly higher diverse biological activities and apparently has significant health benefits.
서 론
재료 및 방법
1. 실험재료
2. 추출물의 제조
3. 총 폴리페놀 함량 측정
4. ABTS 라디칼 제거능
5. DPPH 라디칼 제거능
6. Tyrosinase 저해 활성 측정
7. 세포배양및산화스트레스에대한세포보호효과측정
8. 통계분석
결과 및 고찰
1. 새싹 밀 추출물의 추출 수율 및 총 폴리페놀 함량
2. 새싹 밀 추출물의 항산화 활성
3. 새싹 밀 추출물의 tyrosinase 저해 활성
4. 새싹밀추출물의HepG2 세포내산화스트레스보호효과
요약 및 결론
References
