한국식품영양학회지 Vol. 32 No. 6 (p.662-668)

|연구논문|
강원도 10종 산채류의 항산화 및 인지능력개선 효능 평가

Evaluation of Antioxidant and Cognition Improvement Effects of 10 Wild Vegetables Cultivated in Gangwon Province
키워드 :
wild vegetables extracts,antioxidant activity,cognition improvement effect,acetylcholinesterase,β-secretase

목차

Abstract
서 론
재료 및 방법
   1. 실험재료 및 시약
   2. 총 폴리페놀 함량 측정
   3. 총 플라보노이드 함량 측정
   4. DPPH radical 소거활성 측정
   5. ABTS radical 소거활성 측정
   6. AChE(acetylcholinesterase) 저해 활성
   7. BACE1(β-Secretase1)저해활성
   8. 통계처리
결과 및 고찰
   1. 총 폴리페놀 함량과 총 플라보노이드 함량
   2. DPPH radical 소거활성 측정
   3. ABTS radical 소거활성 측정
   4. Acetylcholinesterase 저해 활성
   5. BACE1(β-secretase) 저해 활성
   6. 상관관계 분석
요약 및 결론
References

초록

The purpose of this study was to investigate effect of methanolic extracts of 10 kinds of wild vegetables cultivated in Gangwon province on antioxidant activity, acetylcholinesterase, and β-secretase inhibitory activities. Results showed that among the wild vegetables, Aralia elata(Miq.) Seem shoot extract exhibited the highest total phenol content (84.65±1.08 mg GAE/g) and total flavonoids content (70.77±0.55 mg RE/g), respectively. The antioxidant activity of wild vegetables extracts was measured by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. Aralia elata(Miq.) Seem shoot extracts had the highest DPPH and ABTS scavenging activity (90.16%, 40.18% at 2 mg/mL). As a result, Aralia elata(Miq.) Seem shoot extract was the most effective in terms of acetylcholinesterase inhibitory activity (35.94% at 1 mg/mL). In the β-secretase activity assay, all 10 kinds wild vegetables extracts showed low inhibitory activity, and Aralia elata(Miq.) Seem shoot extract had highest inhibitory activity among the 10 wild vegetables extracts was 14.99%. Taken together, these results showed that Aralia elata(Miq.) Seem shoot extract has potential cognition improvement impact, suggesting that it may provide an effective strategy for improving cognition.