한국식품영양학회지 Vol. 32 No. 6 (p.717-729)

|연구논문|
국내 재배 상추로부터 UPLC-DAD-QToF/MS를 이용한 페놀화합물 성분 비교 연구

Study on Phenolic Compounds in Lettuce Samples Cultivated from Korea Using UPLC-DAD-QToF/MS
키워드 :
lettuce,phenolic compound,anthocyanin,UPLC-DAD-QToF/MS

목차

Abstract
서 론
재료 및 방법
   1. 실험 재료 및 시약
   2. 페놀산, 플라보노이드 및 안토시아닌 추출
   3. 상추로부터 페놀화합물 구조동정용 라이브러리 제작
   4. UPLC-DAD-QToF/MS 분석
   5. 통계처리
결과 및 고찰
   1. 국내 재배 상추 내 페놀화합물 개별성분 분리 및 동정
   2. 국내 재배 상추 내 30종 페놀화합물의 조성 및 함량비교
요약 및 결론
References

초록

The chemical informs about 70 individual phenolic compounds were constructed from various lettuce samples based on literature sources and analytical data. A total of 30 phenolic compounds including quercetin 3-O-glucuronide, quercetin 3-O-(6''-O- malonyl) glucoside, cyanidin 3-O-(6''-O-malonyl)glucoside, chlorogenic acid and chicoric acid as major components were identified in 6 lettuce samples from Korea using UPLC-DAD-QToF/MS on the basis of constructed library. Among these, quercetin 3,7-di-O-glucoside(m/z 627 [M+H]+), quercetin 3-O-(2''-O-malonyl)glucoside(morkotin C, m/z 551 [M+H]+), quercetin 3-O-(6''- O-malonyl)glucoside methyl ester(m/z 565 [M+H]+), 5-O-cis-p-coumaroylquinic acid(m/z 339 [M+H]+) and 5-O-caffeoylquinic acid methyl ester(m/z 369 [M+H]+) were newly confirmed from the lettuce samples. In total content of phenolic compounds, 4 red lettuce samples(2,947.7~7,535.6 mg/100 g, dry weight) showed higher than green lettuce(2,687.3 mg) and head lettuce(320.1 mg).