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박 덩굴쪼김병 저항성 검정조건 구명 KCI 등재

Determination of an Effective Method to Evaluate Resistance of Bottle Gourd Plant to Fusarium oxysporum f. sp. lagenaria

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생물환경조절학회지 (Journal of Bio-Environment Control)
한국생물환경조절학회 (The Korean Society For Bio-Environment Control)
초록

Fusarim oxysporum에 의한 덩굴쪼김병은 수박 재배에 큰 피해를 일으키는 중요한 병이다. 이 병을 방제하기 위해 덩굴 쪼김병 저항성 박 대목을 이용하는 것이 가장 좋은 방법이다. 본 연구는 박 덩굴쪼김병 저항성 자원을 선발할 수 있는 방법을 확립하기 위해 수행하였다. 효과적인 접종법을 개발하기 위해 접종 후 온도(15, 20, 25, 30°C), 접종 농도(1 X 105, 5 X 105, 1 X 106 and 5 X 106 conidia・mL-1), 접종 시기(파종 7, 10, 13, 16일 후)를 조사하였다. 박 덩굴쪼김병을 접종하였을 때 접종 후 배양 온도나 파종 후 접종 시기의 영향이 적었다. 그러나, 덩굴쪼김병에 저항성인 박의 경우 접종농도가 높을수록 발병이 심하게 나타났다. 따라서, 박 덩굴쪼김병에 대한 효과적인 접종법으로 파종 10일 된 유묘 뿌리를 1 X 105 - 1 X 106 conidia・mL-1 농도로 30분간 접종한 후 감염되지 않은 토양에 옮겨 심고 25°C에서 3주 동안 재배하는 것을 제안한다.

Fusarium wilt caused by Fusarium oxysporum is a devastating disease limiting production of watermelon in Korea. The best way to control diseases is to use resistant gourd rootstock on watermelon. This study was conducted to establish an efficient screening method for resistant bottle gourd to Fusarium oxysporum f. sp. lagenaria. To develop an efficient inoculation method, incubation temperature after inoculation (15, 20, 25, and 30°C), inoculum concentration (1 X 105, 5 X 105, 1 X 106, and 5 X 106 conidia・mL-1), and growth stages of seedlings (7, 10, 13, and 16 days) was investigated. Disease development of Fusarium wilt of bottle gourd was little affected by differences in incubation temperature and growth stages of seedlings. But resistant lines were more susceptible and appeared more severe symptoms at the higher inoculation level. Taken together, we suggest that an effective screening method for resistant gourd plant to Fusarium wilt is to dip the roots of 10-day old seedlings in spore suspension of 1 X 105 - 1 X 106 conidia・mL-1, for 30 min, to transplant the seedlings into a non-infected soil, and then to incubate the inoculated plants in a growth room at 25°C for 3 weeks to develop Fusarium wilt.

목차
Abstract
서 론
재료 및 방법
    1. 덩굴쪼김병균 배양
    2. 박 재배 및 덩굴쪼김병 접종
    3. 발병 및 병조사
결과 및 고찰
    1. 접종원 생산을 위한 균 배양온도 선발
    2. 재배 온도에 따른 덩굴쪼김병의 발생
    3. 접종 농도에 따른 덩굴쪼김병 발생
    4. 접종시기에 따른 덩굴쪼김병의 발생
    5. 박 품종의 덩굴쪼김병 저항성 검정
적 요
저자
  • 김상규(국립원예특작과학원 채소과) | Sang Gyu Kim (Vegetable division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 이옥진(국립원예특작과학원 채소과) | Oak Jin Lee (Vegetable division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 이선이(국립원예특작과학원 채소과) | Sun Yi Lee (Vegetable division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 김대영(국립원예특작과학원 채소과) | Dae Young Kim (Vegetable division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 허윤찬(국립원예특작과학원 채소과) | Yun-Chan Huh (Vegetable division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 안세웅(국립원예특작과학원 채소과) | Se Woong An (Vegetable division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 장윤아(국립원예특작과학원 채소과) | Yoon ah Jang (Vegetable division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 문지혜(국립원예특작과학원 채소과) | Ji hye Moon (Vegetable division, National Institute of Horticultural and Herbal Science, Rural Development Administration) Corresponding author