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발효 균주 탐색을 위한 목장 주변 토양에서 분리한 내염성 미생물의 다양성 및 효소 생산능 분석 KCI 등재

A Research on the Diversity and Enzyme Productivity of Halophilic Microorganisms Isolated from the Soil around the Ranch for the Exploration of Fermentation Strains

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  • URLhttps://db.koreascholar.com/Article/Detail/388244
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산업식품공학 (Food Engineering Progress)
한국산업식품공학회 (Korean Society for Food Engineering)
초록

The diversity and characterization of microorganisms isolated from the soil around several ranches in Korea were confirmed in this study. To isolate halophilic microorganisms, the marine agar medium was basically used and cultivated at 37℃ for several days. After single colony isolation, a total of 116 pure colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rDNA sequencing, indicating that isolated strains were divided into 4 phyla, 23 families, 30 genera and 51 species. To confirm whether isolated strain can be a candidate for the fermentation of diverse food ingredients, amylase, lipase, and protease enzyme assays were performed individually, showing that 92 strains possessed at least one enzyme activity. Especially 4 strains, identified to Jonesia quinghaiensis (isolate name: JSF 19-2), Halomonas alkaliantarctica (isolate name: JSF 21), Bacillus velezensis (isolate name: NWFY-36), and Staphylococcus capitis subsp. urealyticus (isolate name: MSY-5), showed all enzyme activity tested. Moreover, 17 strains showed the ability for auxin production. This result indicated that isolated strains have shown the possibility of the application for the food and feed industries. Therefore, this study has contributed to securing domestic biological resources and the improvement of hydrolytic enzyme activity by using isolated strains.

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저자
  • 이용직(서원대학교 바이오코스메틱학과) | Yong-Jik Lee (Department of Bio-Cosmetics, Seowon University)
  • 신기선(한국생명공학연구원 산업바이오소재연구센터) | Kee-Sun Shin (Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 이상재(신라대학교 식품공학전공&해양극한미생물연구소) | Sang-Jae Lee (Major in Food Biotechnology and Research Center for Extremophiles & Marine Microbiology, Silla University) Corresponding author