In this study, the authors introduce a newly developed flatfish grading system. Owing to the features of flatfish with and wide body, the general types of grading system are not easy to apply for it. Furthermore, the flatfish to be graded is alive such that the existing measurement and grading systems cannot be used for it as well. This study gives a solution for measuring and grading the flatfish with high speed and good accuracy. For this object, the authors developed flatfish measurement and grading system. This system consist of the feeding, conveying, measurement part and sorting part. Especially, the measurement part is made by vision based measuring technique which satisfies the given specification. The result from the experiment shows that the developed system is applicable for measuring and grading the flatfish sizes in variety.