한국환경과학회지 제29권 제3호 (p.241-247)

|ORIGINAL ARTICLE|
환경 독성을 억제하는 느릅 당단백질이 마우스의 분뇨 악취저감 및 사료 효율에 미치는 영향

Anti-ecotoxicological Glycoprotein Isolated from Ulmus davidiana Nakai Inhibits Fecal Malodor and Promotes Feed Efficiency in Mice
키워드 :
Ulmus davidiana Nakai,Glycoprotein,Feed efficiency,Hydrogen sulfide,Ammonia

목차

Abstract
1. 서 론
2. 재료 및 방법
2.1. 시약
   2.2. 느릅 당단백질 분리 및 정제
   2.3. 세포배양 및 MTT 분석
   2.4. 사료 효율 및 장기 독성 측정
   2.5. GOT, GPT 및 LDH 측정
   2.6. H2S 및 NH3 농도 측정
   2.7. 통계처리
3. 결과 및 고찰
   3.1. 느릅 당단백질의 장 세포 보호효과 및 환경독성물질이 유도하는 세포사멸 억제효과
   3.2. 느릅 당단백질이 마우스 장기 및 혈액 독성에 미치는 영향
   3.3. 느릅 당단백질이 마우스 사료 효율에 미치는 영향
   3.4. 느릅 당단백질이 마우스 분내 악취저감에 미치는효과
4. 결 론
REFERENCES

초록

Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprot ein) in regulating fecal malodor and feed efficiency in mice. We found that UDN glycoprotein (200 μg/ml) has an inhibitory effect on the cell death induced by an ecotoxicological endocrine disrupting chemical, bisphenol A, in colon epithelial HT-29 cells. UDN glycoprotein did not show significant differences regarding the weight of ecotoxicity-related organs (liver, heart, kidneys, and spleen) and the levels of serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and lactate dehydrogenase in mice for 2 weeks, compared to the control. Additionally, UDN glycoprotein reduced the levels of hydrogen sulfide and ammonia as markers of fecal malodor in mice. Interestingly, UDN glycoprotein can improve the mouse feed efficiency. In conclusion, our data indicate that anti-ecotoxicological UDN glycoprotein has the ability to increase the feed efficiency and reduce the fecal malodor by maintaining the viability of colonic epithelial cells in mice.