한국식품영양학회지 Vol. 33 No. 3 (p.347-355)

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오미자칠해목 추출물의 과산화수소로 유발된 PC12뇌세포 사멸과 스코폴라민으로 유발된 렛드 동물모델에 대한 개선 효과

Ameliorative Effect of Schisandra chinensis and Ribes fasciculatum Extracts on Hydrogen Peroxide-Induced Neuronal Cell Death in Neuroblastic PC12 Cells and the Scopolamine-Induced Cognitive Impairment in a Rat Model
키워드 :
Schisandra chinensis,Ribes fasciculatum,scopolamine-induced rat,neuroprotection,oxidative stress

목차

Abstract
서 론
재료 및 방법
   1. 시료 제조
   2. 신경세포 배양
   3. 세포 독성 평가
   4. Annexin-propidium iodide(PI) 유세포 분석
   5. 단백질 추출 및 단백질 분석
   6. 실험동물 사육 및 실험설계
   7. Y-maze 실험
   8. 해마의 acetylcholine(Ach) 농도 측정
   9. 통계처리
결과 및 고찰
   1. PC12 세포에서 과산화수소(H2O2)의 처리에 따른 독성평가
   2. PC12세포에서 오미자칠해목 추출혼합물의 독성 및보호효과 확인
   3. 오미자칠해목 추출혼합물 처리에 의한 세포사 확인
   4. Y-maze 실험
   5. 해마의 acetylcholine(Ach) 농도 측정
요약 및 결론
References

초록

Cognitive impairment is considered to be key research topics in the field of neurodegenerative diseases and in understanding of learning and memory. In the present study, we investigated neuroprotective effects of Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts in hydrogen peroxide-induced neuronal cell death in vitro and scopolamine-induced cognitive impairment in Sprague Dawley® (SD) rat in vivo. Apoptotic cell death in neuroblastic PC12 cell line was induced by hydrogen peroxide for 1 hour at 100 μM. However, mixture of SC and RF treatment prevented peroxide induced PC12 cell death with no neurotoxic effects. For in vivo experiment, the effect of SC and RF extracts on scopolamine-induced cognitive impairment in SD rat was evaluated by spontaneous alternation behavior in Y-Maze test. After 30 min scopolamine injection, the scopolamine-induced rats presented significantly decreased % spontaneous alteration and acetylcholine level, compared to non-induced group. However, treatment of SC+RF extracts rescued the reduced % spontaneous alteration with acetylcholine concentration from hippocampus in scopolamineinduced rats. These results suggested that mixture of SC and RF extract may be a potential natural therapeutic agent for the prevention of cognitive impairment.