Korean Journal of Environmental Biology Vol. 38 No. 2 (p.248-253)

유동세포분석에 의한 참전복 (Haliotis discus hannai) 세포내 DNA 함량 분석 최적화

A flowcytometric determination of DNA content in Pacific abalone, Haliotis discus hannai cell
키워드 :
abalone (Haliotis discus hannai),DNA content,flowcytometry,tissues


서 론
재료 및 방법
결과 및 고찰
적 요


The level at which analyses of DNA content might contribute more significantly to the genetic mechanisms of evolution lies in the events of speciation. The object of this study was to investigate the DNA content of abalone (Haliotis discus hannai) and determine the optimal tissue samples for measuring the DNA content of abalone by flowcytometry without fixation. The DNA content (pg/nucleus) of gill tissue (2.5±0.08), which was contaminated with protozoa, was significantly lower than that of muscle tissue (3.2±0.02), mantle tissue (3.2±0.02) (p<0.05), and a standard reference standard, while the DNA contents of muscle tissue and mantle tissue were higher than that of the standard reference. Considering the results of this study, DNA content analysis with flowcytometry is an acute and rapid method by which muscle tissue and mantle tissue are the most appropriate sample for measuring the DNA content of abalone without fixation.