韓國食生活文化學會誌 제35권 제3호 (p.278-284)

국내 다소비 식품의 biotin 함량 모니터링

Monitoring of Biotin Content in Frequently Consumed Foods in Korea
키워드 :
Biotin,frequently consumed foods,immunoaffinity column,high-performance liquid chromatography

목차

Abstract
I. 서 론
II. 연구 내용 및 방법
   1. 재료 및 시약
   2. 시료의 biotin의 추출 및 전처리
   3. Biotin의 HPLC 분석
   4. Biotin 분석법 검증
   5. Biotin 분석의 내부 분석품질관리
   6. 통계처리
III. 결과 및 고찰
   1. 식물성 식품의 biotin 함량 모니터링
   2. 동물성 식품의 biotin 함량 모니터링
   3. Biotin 분석법 검증 및 내부 분석품질관리
IV. 요약 및 결론
References

초록

In this study, biotin (vitamin B7) contents of frequently consumed foods in Korea were determined by using immunoaffinity column in conjunction with high-performance liquid chromatography (HPLC). The biotin contents of 24 foods of plant origin and 27 foods of animal origin were selected. The highest biotin contents in frequently consumed foods of plant origin were found in red beans (Huinguseul; 11.475 μg/100 g). On the other hand, biotin was not detected in any varieties of sorghum. For frequently consumed foods of animal origin, salted pollack roe (7.486 μg/100 g) showed the highest biotin content. However, beef and fish contained less biotin. All biotin analyses were conducted under analytical quality control. The limits of detection and limits of quantification of biotin were 0.007 and 0.023 μg/100 g, respectively, and the accuracy/recovery percentage was 95.35-105.02%. The precision values were 4.041% (repeatability) and 3.835% (reproducibility). Taken together, our data provide reliable data on the biotin contents of frequently consumed foods in Korea.