농업생명과학연구 제54권 제4호 (p.37-42)

The Optimal In Vitro Culture Condition for Shoot Multiplication of Evergreen Oak Tree, Quercus myrsinaefolia Blume

키워드 :
Q. myrsinaefolia,In vitro culture,Asexual propagation,Adventitious roots

목차

Abstract
Introduction
Materials and Methods
Results
Discussion
References

초록

The evergreen oak tree, Quercus myrsinaefolia Blume, is not only economically important for wood, medicine, landscape trees, etc., but also becoming more important in terms of ecology due to climate change. However, asexual reproduction was difficult, so this study was conducted to establish the optimum conditions for micropropagation by shoot multiplication. The surface sterilized seeds of Q. myrsinaefolia were successfully germinated in WPM basal medium. BAP (1.0 mg/L) treatment was most effective for inducing multiple shoots. The highest induction rates of adventitious roots from the multiple shoots was shown in the treatment of 1.0 mg/L NAA. Both MS and WPM medium were most effective for growth of multiplied plantlets. For ex vitro acclimatization, the survival rates of multiplied plantlets were 100% in vermiculite and commercial soil. The results of this study can be used for proliferation and supply, and establishment of ex situ conservation of Q. myrsinaefolia elite trees.