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pp.819-826
Production of Bacterial Cellulose (BC) by Gluconacetobacter sp. A5 was studied in shaken culture using different cost-effective carbon sources and its structural and mechanical properties were evaluated. Glycerol showed the highest level (7.26 g/l) of BC production, which was about three times higher than the yield in glucose medium. BC production depended not only on the decrease in pH, but also on the ability of Gluconacetobacter sp. A5 to synthesize glucose from different carbon sources and then polymerize it into BC. All BC produced from different carbon sources exhibited a three-dimensional reticulated structure consisting of ultrafine cellulose fibriles. Carbon sources did not significantly change the microfibrile structure of the resulting BC. BC produced from glucose medium had the lowest water-holding capacity, while BC from molasses medium had the highest. XRD data revealed that all BC were cellulose type І, the same as typical native cellulose. The crystalline strength of BC produced in glucose medium was the highest, and that in molasses medium was the lowest. Our results suggest that glycerol could be a potential low-cost substrate for BC production, leading to the reduction in the production cost, and also to produce BC with different mechanical properties by selecting appropriate carbon source.
1. 서 론
2. 재료 및 방법
2.1. 사용균주 및 배양조건
2.2. 경제적 탄소원 탐색
2.3. 세균 섬유소의 생산량 측정
2.4. 세균 섬유소의 3차원적 미세구조 관찰
2.5. 세균 섬유소의 결정 구조 분석
2.6. 세균 섬유소의 수분 보유력 측정
2.7. 세균 섬유소의 역학적 특성 분석
3. 결과 및 고찰
3.1. 탄소원이 세균 섬유소의 생산에 미치는 영향
3.2. 탄소원이 세균 섬유소의 미세구조에 미치는 영향
3.3. 탄소원이 세균 섬유소의 수분 보유력에 미치는 영향
3.4. 탄소원이 세균 섬유소의 결정구조에 미치는 영향
3.5. 탄소원이 세균 섬유소의 역학적 특성에 미치는 영향
4. 결 론
REFERENCES
