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잠재적 미생물 농약으로서 다양한 식물성장 촉진 활성을 가진 siderophore 생산 세균의 분리와 특성 KCI 등재

Isolation and Characterization of Siderophore-Producing Bacteria with Various Plant Growth-Promoting Abilities as a Potential Biocontrol Agent

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  • URLhttps://db.koreascholar.com/Article/Detail/399504
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한국환경과학회지 (Journal of Environmental Science International)
한국환경과학회 (The Korean Environmental Sciences Society)
초록

To develop eco-friendly microbial inoculants, siderophore-producing bacteria were isolated and identified, and their production characteristics and plant growth-promoting abilities were investigated. A strain S21 was isolated from rhizosphere of Korean perilla (Perilla frutescens) and identified as Enterobacter amnigenus by phenotypic properties and 16S rRNA gene sequencing. The highest siderophore production was obtained in a medium containing 0.5% fructose, 0.1% urea, 0.5% K2HPO4 and 0.1% succinic acid. By using this improved medium, siderophore production increased by 2.5 times compared to that of basal medium. The strain S21 showed insoluble phosphate solubilizing, ammonification and antifungal activities, and also produced hydrolytic enzymes (protease and lipase), indoleacetic acid and 1-aminocyclopropane-1-carboxylate deaminase. Our data suggest that E. amnigenus S21 is a potential candidate that can be used as eco-friendly biocontrol agent and biofertilizer.

목차
Abstract
1. 서 론
2. 재료 및 방법
    2.1. Siderophore 생산균주의 분리 및 동정
    2.2. Siderophore 생산에 영향을 미치는 배지성분 조사
    2.3. Siderophore의 정량분석
    2.4. 식물성장 촉진 활성 조사
3. 결과 및 고찰
    3.1. 공시균주의 분리 및 동정
    3.2. Siderophore 생산을 위한 배지조성 개선
    3.3. 공시균주의 식물성장 촉진 활성
4. 결 론
REFERENCES
저자
  • 최승훈(부산대학교 생명환경화학과 및 생명산업융합연구원) | Seunghoon Choi (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University)
  • 유지연(부산대학교 생명환경화학과 및 생명산업융합연구원) | Ji-Yeon Yoo (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University)
  • 박성진(부산대학교 생명환경화학과 및 생명산업융합연구원) | SungJin Park (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University)
  • 박민주(부산대학교 생명환경화학과 및 생명산업융합연구원) | MinJoo Park (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University)
  • 이오미(농림축산검역본부 식물검역기술개발센터) | O-Mi Lee (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea)
  • 손홍주(부산대학교 생명환경화학과 및 생명산업융합연구원) | Hong-Joo Son (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University) Corresponding author