The status of probiotics is increasing worldwide and the range of application of probiotics is also being extended to the livestock industry. The use of probiotics in the swine industry aims to improve animal health and productivity by forming a healthy gut microbiota. Intensive pig breeding, a common breeding method in modern society, causes physiological stress in pigs, resulting in imbalance of the intestinal microbiota and dysfunction of the intestinal barrier. We conducted a scientific research on the properties of probiotics for multi-strain probiotics (MSP) and observed the economic benefits and efficiency of culturing MSP through a self-cultivation system in livestock. Initially, we observed alteration occurred by MSP application in the gut microbiota of pig. MSP were characterized to have resistance to digestive juices such as gastric acid and bile, followed by colonization in the target organ, the gastro-intestinal tract (GIT). In addition, MSP was also confirmed to have antibacterial ability against pathogenicity that most frequently infected in the swine industry and showed low resistance to antibiotics which means guaranteed stability when added to feed. The growth rate of probiotics in the optimized medium used in the self-cultivation system was suitable for efficiently and economically culturing and feeding pigs with high concentrations of probiotics, considering the ingredient price and the growth efficiency. We observed the significant alteration of gut microbiota of pig by application of MSP. Importantly, the MSP supplementation significantly increased the beneficial bacteria genus of Bifidobacterium that confer a health benefit to the host in pig GIT, whereas decreased the number of harmful bacteria including coliforms. In addition, MSP influenced on the uniformity of gut microbiota at both of sow and weaning pigs. Taken together, the application of MSP with high concentration of probiotics using self-cultivation system may critically improve the pig health by regulation intestinal microbiota.

Abstract
서 론
재료 및 방법
    사용 균주
    내산성 및 내담즙성 평가
    항균 활성
    항생제 내성
    Caenorhabditis elegans 부착 능력 평가
    Multi-strain probiotics(MSP)의 농장 급여 및 사료 분석
    농장 자가배양시스템을 위한 배지 조성
    장내 미생물 분석
결 과
    MSP의 probiotic 특성
    MSP의 항균 활성과 항생제 저항성 평가
    농장 자가배양시스템을 이용한 MSP의 급여
    MSP급여로 인한 돼지 장내 microbial composition의 변화
고 찰
감사의 글
References

• 이웅지(서울대학교 농업생명과학대학 농업생명과학부) | Woong Ji Lee (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University)
• 윤보현(전북대학교 우유 유전체 연구소) | Bohyun Yun (Institute of Milk Genomics, Jeonbuk National University)
• 이학교(전북대학교 농업생명과학대학 동물생명공학과) | Hak Kyo Lee (Department of Animal Biotechnology, Jeonbuk National University)
• 허재영(전북대학교 농업생명과학대학 동물생명공학과) | Jaeyoung Heo (Department of Animal Biotechnology, Jeonbuk National University)
• 김영훈(서울대학교 농업생명과학대학 농업생명과학부) | Younghoon Kim (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University) Corresponding author
• 오상남(전주대학교 의과학대학 바이오기능성식품학과) | Sangnam Oh (Department of Functional Food and Biotechnology, Jeonju University) Corresponding author