간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
2019 Spring International Conference of KSAE (2019년 4월) 299건
Oral Presentation : Ecology
61.
2019.04
구독 인증기관·개인회원 무료
Seung-Gyu Lee, Cheolhak Kim, Alexander V. Kuprin, Jung-Hoon Kang, Bong-Woo Lee, Seung Hwan Oh, Jongok Lim
An investigation on the habitation of Callipogon relictus Semenov was carried out in Gwangneung forest, Korea and Ussurisky nature reserve, Russia. During the investigation period (2007–2018), a total of 25 emergence holes, 10 pupal chambers, 10 adults, and two larvae of C. relictus were identified in Gwangneung forest. In this presentation, we provide biological information on habitation of C. relictus in Gwangneung forest by comparison and combination of survey results in Ussurisky nature reserve, in 2015 and 2018. In addition, the distribution range of C. relictus is also reviewed in the Korean Peninsula with a new location.
62.
2019.04
구독 인증기관·개인회원 무료
갈색날개매미충은 현재 급속도로 확산하고 있으며 그 피해는 기하급수적으로 증가하고 있다. 이번 연구는 이러한 갈색날개매미충의 현재와 미래 분포를 CLIMAX와 MaxEnt를 사용하여 예측하였다. 또한 두 개의 프로그램에서 변수 선정은 사용된 환경인자와 갈색날개매미충 발생과의 공간적 상호 관계를 SADIE로 분석하여 사용함으로써 발생에 영향을 주는 요소만을 분석에 이용하였다. CLIMAX의 경우 중국 저장성의 기후 요소와 한국 전역의 기후 요소를 Climate Matching을 사용하였고, MaxEnt의 경우 상관관계 분석과 공간자료 분석에 의해 선정된 11개 환경 요소를 이용하여 갈색날개매미충의 현재 및 미래 분포를 예측하였다. 연구결과 한국 전역은 갈색날개매미충 발생에 적합한 것으로 나타났으며, 한국의 서쪽 지역이 동쪽 지역보다는 상대적으로 발생 적합성이 높았으며 동쪽으로 점차 이동할 가능성이 높은 것으로 나타났다. 이번 연구 결과는 갈색날개매미충의 방제 전략을 구축하는데 도움을 줄 뿐만 아니라 보다 유의미한 종분포 모델 개발을 위해 공간자료 분석의 사용법을 제시하였다.
63.
2019.04
구독 인증기관·개인회원 무료
The Wolbachia bacterium, one of the most prevalent endosymbiotic bacteria, is known to induce reproductive anomalies in various invertebrate taxa. We investigated Wolbachia infection frequency in 203 Coleopteran insects collected from Korea. Among them 26 species (12.8%) across families proved to harbor Wolbachia. The phylogenetic trees of two Wolbachia specific genes imply that there have been complex horizontal gene transfers and recombination events within and between divergent Wolbachia subgroups. Interestingly, we also find that the infection frequency is noticeably low and incongruent at the family level when compared to other literature cases of Wolbachia infection in beetle species.
64.
2019.04
구독 인증기관·개인회원 무료
Wildfire, which may effect on the spatial distribution of many insects, is one of important disturbances in forests. Because Monochamus beetles are known as vector insects of pine wood nematodes in Korea and burned pine trees can be used as oviposition habitats for Monochamus beetles, we investigated the changes in spatial and temporal distributions of M. alternatus and M. saltuarius across fire severity classes. We also studied other saproxylic beetles, such as cerambycid, scolytid, and bark beetles, which are potential insect pests for pine trees. Study area was classified by fire severity, such as unburned, light (<25% burned), moderate (25~75%), and severe (>75%). Spatial distribution of saproxylic beetles were significantly related to the fire severity.
65.
2019.04
구독 인증기관·개인회원 무료
Vespa velutina is a predatory hornet distributing in SouthEastern part of Asia and became invasive in early 2000s; invading into Korea in 2003 and France in 2004. Its distribution pattern showed typical species-invasion curve type with slow progress in the beginning and rapid expansion on ward. We determined the geographic spread of V. velutina with CLIMEX modeling. The model analysis indicates that the ecoclimatic indices (EI) increased as the projection year increased based on RCP8.5 scenario. Then the predicted EI values were further regressed with the field collected data from 2018. Vespa velutina population sizes were estimated from 230 points of sampling over the country and the sampling data were correlated with predicted EI values. The results indicated that partial contribution of the climate factors for its abundance. Also this could be an usual biological indicator of climate change in agroecosystem in Korea. Given the important risk and impact on beekeeping, socio-biological as well as ecosystem and biodiversity levels, careful monitoring of phenology, range expansion and preventive efforts mitigating the impact are further required.
Oral Presentation : Pest Control
66.
2019.04
구독 인증기관·개인회원 무료
소나무재선충의 매개충인 솔수염하늘소(Monochamus alternatus Hope)와 북방수염하늘소(Monochamus saltuarius Gebler) 암수 성충의 더듬이 감각기를 주사전자현미경로 관찰하여 형태적 특성과 분포를 조사하였다. 두 매개충의 암수 성충 더듬이는 자루마디(scape), 팔굽마디(pedicel)와 9개의 채찍마디(flagellum)으로 이루어져 있었다. 더듬이 표면에 있는 감각기의 종류에는 종 간, 암수 간에 차이가 나지 않았으며 Chaetica 3 종류, Trichodea 2 종류, Basiconica 3 종류, Coeloconica 1 종류로 구분되었다. 이 중 Basiconica 3 종류는 모두 후각 수용체, Trichodea는 미각 수용체, 나머지는 기계적 감각기로 추정되었다. 감각기의 더듬이 내 분포를 보면 전체적으로 Chaetica 감각기가 가장 많았다. Basiconica 감각기는 채찍마디에만 존재하였으며 끝으로 갈수록 수가 많아졌다. Trichodea 감각기와 Coeloconica 감각기는 각 마디별로 밀도가 일정하였다.
67.
2019.04
구독 인증기관·개인회원 무료
This study was conducted aiming to figure out two things. First, knowledge of the change in spatial distribution of Tetranychus urticae depending on how to control it (using pesticide or natural enemy). Second, spatial association of T. urticae and Phytoseiulus persimilis in biocontrol plot (B.P). The data was analyzed by spatial analysis by distance indices (SADIE) using global aggregation index, Ia . Ia values were 0.77-1.37 in conventional plot (C.P) and 0.88-1.68 in B.P, respectively. However, the fluctuation level of Ia in B.P was higher than C.P. Therefore, the results indicated that there was a clear spatial pattern change in B.P, i.e. prey’s spatial distribution is affected by natural enemy. And spatial association analysis showed that T. urticae and P. persimilis have positively associated. It means that T. urticae is relatively low mobile prey, and P. persimilis is relatively high mobile predator.
68.
2019.04
구독 인증기관·개인회원 무료
Mi Rong Lee, Dongwei Li, Jong Cheol Kim, Sihyeon Kim, So Eun Park, Sehyeon Baek, Tae Young Shin, Jae Su Kim
The longhorned tick, Haemaphysalis longicornis, is one of the vectors of severe fever with thrombocytopenia syndrome virus (SFTSV) in human. The use of pyrethroid insecticides induced pest resistance and environmental residual toxicity. Here in this work, our interest was given to the selection of highly virulent fungi against longhorned tick. A total of 101 fungal pathogens were assayed by dipping the nymph stage of ticks into a conidial suspension. Interestingly of the several species, one species showed high virulence and mycosis were observed in 7-15 days. Highly virulent strains were selected, and semi-field experiments were conducted. As a result, the control efficacy of the isolate was over 80% at 30 days of treatment. This work suggests that entomopathogenic fungi could be used to effectively control longhorend ticks.
69.
2019.04
구독 인증기관·개인회원 무료
Frankliniella occidentalis is a major pest in agriculture. Following overuse of insecticides, high resistance has developed due to its high reproduction rate and short generation time. To control the resistant strains of the thrips, the ingestion RNAi- based control was established. A total of 67 genes were selected, and their double-stranded RNAs (dsRNA) were delivered to thrips via the leaf disc-feeding method. Among the genes screened, the dsRNA of Toll-like receptor 6 (TLR6) and coatomer protein subunit epsilon (COPE) resulted in the highest mortality (3.8- and 2.8-fold faster LT50 compared to control, respectively) when ingested by thrips. The dsRNA-fed thrips showed 53% and 83% reduced transcription levels of TLR6 and COPE, respectively. This result demonstrates that the observed mortality of thrips following dsRNA ingestion was due to RNAi, and this lethal genes can be employed as a practical tool to control thrips in the field.
70.
2019.04
구독 인증기관·개인회원 무료
Females of adzuki bean beetle (ABB), Callosobruchus chinensis (L.), a cosmopolitan field-to-storage pest of legumes, release sex pheromone consisting of two isomeric components, 2Z-homofarnesal and 2E-homofarnesal. Two day old virgin ABB adults were exposed to sub-lethal fumigation doses (LC25=0.0056 and LC50=0.0076 mgL-1) of phosphine (PH3) to evaluate the effects on adult longevity, fertility and sex pheromone production. Longevity (female; P < 0.001, male; P < 0.001), fecundity (P < 0.001) and sterility (P < 0.001) were significantly affected. The GC-MS for solid phase micro extraction exhibited the significant reduction of release of both of the pheromone components; 2Z-homofarnesal (P < 0.0038) and 2E-homofarnesal (P = 0.0009) when the females were exposed to LC25 dose of PH3. This study unveils the detrimental effects of PH3 fumigation on some of the biological parameters of ABB.
71.
2019.04
구독 인증기관·개인회원 무료
The insecticidal activity of Bacillus thuringiensis subsp. israelensis (Bti) is due to synergistic interactions among its four major proteins (Cry4Aa, Cry4Ba, Cry11Aa, Cyt1Aa), while the activity of Lysinibacillus sphaericus (Ls) is due to a binary toxin (Bin) consisting of a toxin domain, BinA, and a midgut receptor-binding domain, BinB. Although used commercially for almost three decades, reports of mosquito resistance to Bti have been rare. However, levels of resistance greater than 10,000-fold to Bin have been reported where Ls has been used intensively for mosquito control. Cyt1Aa is a lipophilic protein, and in previous studies we showed it delays the evolution of resistance to the Cry proteins of Bti, and can overcome high levels of resistance Bin. In a previous study, we fused Cyt1Aa to BinA, using the lipophile as a broad-spectrum binding domain and showed that the Cyt1Aa-BinA chimera was remarkably toxic to five major vector species of mosquitoes, Anopheles gambiae, An. stephensi, An. quadrimaculatus, Bin-sensitive and Bin-resistant strains of Culex quinquifasciatus, and Aedes aegypti, the latter not normally sensitive to Ls. However, toxicity against Aedes aegypti was not as high as against other mosquito species. Here we show that introducing another highly mosquitocidal protein, Cry11B from B. thuringiensis subsp. jegathesan, enhances the chimera’s toxicity against Ae. aegypti significantly but not against Cx. quinquefasciatus.
72.
2019.04
구독 인증기관·개인회원 무료
Jae Su Kim, Mi Rong Lee, Jong Cheol Kim, Sihyeon Kim, So Eun Park, Dongwei Li, Sehyeon Baek, Minsung Jo, Tae Young Shin, Leila Gasmi, Junheon Kim
The serious emergence of chemical-mediated residual toxicity and insect resistance have been enforced the regulation of synthetic pesticides. Future decisions to select more realistic control options probably depend on the speed of technological development in chemical and biological pesticides. Now, a strategic collaboration between synthetic pesticides and biopesticides has been progressed, such as distribution and R&D in collaboration and M&A for obtaining microbial resources. Recently registered microbial pesticides are entomopathogenic fungi in pest management. A concept of e-biopesticide could be properly combined with digital agriculture and accelerate the use of biological control agents in the future farming.
Oral Presentation : Physiology, Molecular Biology
73.
2019.04
구독 인증기관·개인회원 무료
Bemisia tabaci is a species complex consist of about 40 cryptic species. This species is also a vector of at least 100 begomovirus including Tomato yellow leaf curl virus (TYLCV). TYLCV transmitted by B. tabaci in a circulative propagative manner but its molecular mechanism is remain unclear. We investigate the transcriptional response of B. tabaci MED to TYLCV infection using next generation sequencing (NGS) analysis. In total 16757 differentially expressed genes between viruliferous and non-viruliferous whiteflies were analyzed. Among them 289 transcripts found significant, where 116 up-regulated and 173 down-regulated. Our results reveal the whitefly-TYLCV relationship and provide important molecular information about virus transmission of vector insects.
74.
2019.04
구독 인증기관·개인회원 무료
Insulin/IGF signaling (IIS) regulates multiple physiological processes such as larval growth, reproduction, and life span in many organisms including legume pod borer, Maruca vitrata (Lepidoptera: Crambidae). RNA interference of IIS components, insulin receptor (InR) and Forkhead Box O (FOXO), impaired larval growth and female reproduction. To further validate the physiological roles of InR and FOXO, we generated knock-out (KO) mutants using CRISPR/Cas9-mediated genome-editing technology. Both KO mutants exhibited delayed larval growth and reduced pupal and adult body sizes. In conclusion, these results demonstrated the critical role of insulin signaling (IIS) pathway to control M. vitrata growth and development.
75.
2019.04
구독 인증기관·개인회원 무료
Prostaglandin E2(PGE2) is an autocrine and paracrine signal in insects and other animals. Its signal pathways in target cells are well understood in mammalian system but not in insects. Here, we assessed PGE2 signaling in hemocytes of Spodoptera exigua through knocking-down of signal component genes by RNA interference (RNAi) and knocking-out (KO) of PGE2 receptor using CRISPR-Cas9. From S. exigua transcriptome, we selected hemocyte signaling components and analyzed their functions in cellular immune responses through RNAi. KO mutant against PGE2 receptor exhibited severely hampered larval development and adult fecundity.
76.
2019.04
구독 인증기관·개인회원 무료
Sacbrood virus (SBV) caused significant colony collapse in Korean Apis cerana. Considering that hygienic behavior in honey bees confers colony-level resistance against brood diseases, we utilized this trait for selecting A. cerana colonies. In addition, the brood survival rate was evaluated after colonies were SBV-inoculated. Over four selective generations, dead brood removal and brood survivorship in selected colonies were higher than those in the unselected colonies (P < 0.01, 99.3 vs. 89.9% for removal of pin-killed pupae; P < 0.01, 99.0 vs. 63.9% for removal of SBV-killed larvae; and P < 0.01, 70.0 vs. 9.2% for brood survivorship). Following SBV-inoculation, selected colonies showed an increase in the number of surviving pupae and adults, whereas unselected colonies collapsed mostly. Our results confirm the feasibility of selecting SBV-resistant A. cerana.
77.
2019.04
구독 인증기관·개인회원 무료
Laila Gasmi, Sehyeon Baek, Jong Cheol Kim, Mi Rong Lee, Sihyeon Kim, So Eun Park, Dongwei Li, Tae Young Shin, Jae Su Kim
Beauveria bassiana is widely studied for its potential as biopesticide. However, little is known about the factors to influence genetic diversity among isolates. In this work, we aimed to study the gene diversity of 42 isolates to figure out the impact of sequences variability on various biological features. The accumulated data showed no correlation between sequence variability and the fungal geographic localization. Meanwhile, weak to strong correlations have been obtained between the sequence diversity of various genes and the studied biological features. Interestingly, Biotrophy-associated gene 2 gene variability was correlated with all the studied biological features, being an important marker to determine isolates of interest for biological control. Therefore, the obtained data would serve as a database to focus on interesting genes related to improving or screening highly virulent isolates as biological control agents.
78.
2019.04
구독 인증기관·개인회원 무료
Tae Young Shin, Mi Rong Lee, Se Jin Lee, Jong Cheol Kim, Sihyeon Kim, So Eun Park, Dongwei Li, Sehyeon Baek, Laila Gasmi, Jae Su Kim
A species of Beauveria bassiana is widely used for biological pest management in many countries. Many efforts have been given to figure out the clear fungal mode of action to enhance the insecticidal activity. Homologous recombination (knock-out) or hairpin RNA (knock-down) is popularly used in fungal gene function study, but gene cloning and generation of knock-out or -down mutants takes long time or temporarily knock-downed. Here in this work, we used previously generated egfp-expressing B. bassiana strain (Bb-egfp #3) and integrated dsegfp to the Bb-egfp #3 using a protoplast integration method. This work suggests that protoplast integration with dsRNA possibly generate significantly reduced gene expression in B. bassiana and the reduction is quite stable over generations which provide easy of functional study for fungal mode of action.
79.
2019.04
구독 인증기관·개인회원 무료
To explore the origins of coastal colonization within the tribe Athetini Casey, we present a revised molecular phylogeny. The dataset comprised partial mitochondrial COI, COII, 16S rDNA, NADH1, partial nuclear 18S rDNA and 28S rDNA. We chose a total of 95 species in 51 genera, including 14 coastal species in eight genera and 21 outgroup species. The ecological association of species with a coastal habitat was mapped onto a phylogeny to assess the evolution of habitat specialization in the Athetini lineage. The results reveal that five independent origins (clades A–E) of coastal colonization have occurred throughout the tribe Athetini. The following changes in classification are proposed: (i) Adota minuta Lee and Ahn is removed from the genus Adota and tentatively included in Atheta (Badura); (ii) The genus Saphocallus Sharp is transferred from Athetini to Geostibini.
80.
2019.04
구독 인증기관·개인회원 무료
The Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae), produces final instar larvae of overwintering stage in response to decreasing day-length and temperature, and then those larvae pupate after about 6-8 month. In this study, we investigated a few properties related to cold hardiness during overwintering of the insect. Overwintering larvae collected in December could survive at least for 16 days at -20oC, while all larvae of a laboratory colony, which was reared at 25oC, 15L/9D, died within 2 hours. Supercooling points of overwintering larvae were below -20oC, and the temperatures were significantly lower than those (ca. -10oC) of the laboratory colony. It was estimated that higher content of hemolymph glycerol is responsible for cold-hardiness as a cryoprotectant during overwintering of O. furnacalis.
