최근 급속하게 발전하고 있는 유전자교정기술은 식물이 생산하는 특정 이차 대사산물의 집적을 유도하기 위한 식물대사공학연구에 아주 유용하게 이용되고 있다. 특히 이들 기술 들을 이용하여 만든 유전자교정 작물 중에 일부는 외래 DNA 단편이 잔존 하지 않기 때문에 기존의 유전자변형작물의 안전 관리규정에 적용되지 않을 수 있다는 장점이 있다. 따라서 본 리뷰는 phenylpropanoid 대사과정에 의하여 합성되는 다양한 종류의 이차대사산물을 집적시키기 위한 유전자교정 기술의 적용 연구결과 들을 조사하였다. 먼저, phenylpropanoid 생합성 대사과정에 관여하는 다양한 효소를 암호하는 유전자들을 목표로 하여 식물의 종에 따라 특이하게 집적되는 flavonoids, anthocyanin, 수용성 tannins, 로즈마린산 등의 집적을 유도하거나 화색을 변경하는 등의 성공적인 연구결과들을 검토하였다. 또한, phenylpropanoid 대사과정의 조절에 최종조절 스위치 역할을 하는 수많은 종류의 MYB 전사인자를 암호 하는 유전자를 목표로 하여 CRISPR 유전자교정을 시도한 연구결과들로부터 식물의 이차세포벽 형성에 관여하는 lignin, 물관부, cellulose 등의 생합성 조절 기작을 이해하고 MYB family에 속하는 수많은 종류의 유전자에 대한 개별적인 기능 분석 연구결과들을 조사 분석하여, 문제점 및 향후 연구 방향 등을 검토하였다.

서로 다른 감각 정보의 불일치가 가상현실 멀미를 유발하는 것으로 알려져 있다. 본 연구에서는 트레드밀 기반의 가상현실 보행을 통해 감각 정보 일치의 멀미 완화 효과를 검증하고 나아가서 가상현실에서의 광학 흐름(optic flow) 단서에 의한 시각적 속도감과 트레드밀 보행을 통한 보행 속도감의 불일치가 멀미 완화 효과에 미치는 영향을 검증 하였다. 실험1에서는 트레드밀 보행의 멀미 완화 효과를 검증하기 위해 HMD를 통해 앞으로 이동하는 가상현실 체험을 하면서 가만히 서 있을 때와 트레드밀 보행을 할 때의 멀미 수준을 비교하였다. 실험 결과 가상현실 속에서 이동을 하며 트레드밀 보행을 할 때 더 적은 멀미를 느끼고 실재감과 몰입감은 증가하였다. 실험2와 실험3에서는 가상현실 속에서 이동하는 속도에 대한 시각적 속도감과 트레드밀 보행의 속도감을 조작해 두 속도감이 일치할 때와 불일치할 때의 멀미 수준을 비교하였다. 흥미롭게도 참가자들은 시각적 속도감이 보행속도감보다 빠르게 느껴지는 불일치 조건에서 일치조건보다 더 낮은 멀미를 경험하였다. 이와 같은 실험결과는 가상현실 속에서 이동할 때 시각적 정보와 일관되게 트레드밀 보행을 하게 하는 것이 가상현실 멀미를 완화시키지만 속도감의 일치가 필수적인 것이 아님을 시사한다.

The purpose of this study was to evaluate the appearance, physicochemical, physical, and fermentation properties of the fermented soybean produced by manufacturing with inoculation the different types of microbial strains. The strains were inoculated by the NSI (natural strains inoculation), and the SSI (selective strain inoculation) were treatments. The appearance showed differences in color, viscous substance, and hardness depending on strains inoculation and fermentation duration. The pH, and total acidity were 6.40~7.26%, and 0.10~0.39% respectively with differences depending on the samples. The moisture content as the fermentation duration increased, the NSI (56.03~57.66%) decreased and the SSI (56.71~58.63%) increased. The physical characteristics of the hardness increased as the fermentation duration increased for the NSI and the SSI decreased. The color values for the L, a, and b values were 47.64~58.56, 7.15~9.08, and 12.41~17.30, respectively. The α-amylase and protease activities of the SSI were the highest among all treatments. The total viable cell counts of the fermented soybean products by strains were 5.02 to 9.77 log CFU/g, and SSI (fermentation, 48 hours) was the highest. The amino-type nitrogen contents of all samples were 301.62~746.97 mg% and the SSI showed the highest content. The amino acid had the highest glutamic acid content.

This study examined Korean employees’ meal structure characterized by mealtime, meal places, and companions. The data from 19,692 time diaries, recorded by 9,846 employees aged 19-64 years for two days in the 2014 Korean Time Use Survey, were analyzed for working days and non-working days. Approximately two-thirds of Korean employees ate meals three times a day on both working and non-working days. The breakfast and lunch-times on working days were distributed within two hours, but the dinner time on working days and all three meals on non-working days were dispersed across a three-hour range. Male employees spent three minutes on meal preparation on working days, whereas females spent 30 minutes. On working days, 88% of breakfasts and 67% of dinners were eaten at home. For lunches, 46% were eaten at restaurants, and 42% were at workplaces. The breakfast on working days showed the highest percentage of eating alone (40%) and the dinner on non-working days appeared highest in the percentage of eating with families (69%). The characteristics of Korean employees’ meal structure appeared different on working days and non-working days. Such characteristics should be considered in the process of planning nutrition policies and programs for employees.

Effect of 6kg large unit with the carton box (20% open ratio) and MA box (10,000cc·m-2·day-1·atm-1 oxygen transmission rates modified atmosphere package), and the 100g small unit with MA film on asparagus sensory quality were evaluated. The CO2 concentration depended largely on the packing unit and maintained at around 3% in small MA packages, whereas in the MA box increased to 12%. Ethylene concentration rapidly increased until after 3 days of storage in MA packages and then decreased to maintain 5μL·L-1. Unrelated to the unit size, the lower weight loss was obtained in MA packages. A significant difference in visual quality was shown since the 15th day, the best and worst were the MA box and small MA package on the finish day. Off-odor was the highest in small MA packages and the lowest in the carton box (< 3.0). Although there was no significant difference in firmness among all treatments, the packages showed the highest firmness in tips and stems, respectively. The sugar content and hue angle decreased during storage, but there was no statistical difference in all treatments. EL was lowest and highest in small MA package and carton box, respectively. On the 10th day, the total aerobic bacteria was lowest in small MA packages, but no significant difference on the 20th day. E. coli was not found in all treatments on the 10th day, while it was the lowest in the MA box on the 20th day. The mold and yeast were not observed during the whole storage. Based on the above results, the carton box packaged with 10,000cc OTR film was more effective in maintaining the quality of green asparagus with the suitable CO2 concentration for asparagus cold storage.

In this study, the properties of the substances fermented soybean producted by manufacturing with several Aspergillus strains were investigated. The five soybean cultivar that includes miso, jinpung, pyeongwon, cheonga and chamol were used in this experiment. The pH and total acidity were 6.30~6.66%, and 0.27~0.48%, respectively with differences depending on the samples. The color values for L value, a, and b value were 60.28~69.80, 0.64~3.68, and 11.48~31.21, respectively. The amylase activities, protease activities, and amino-type nitrogen of the jinpung samples were the highest among all cultivars. The mold counts the fermented soybean products by cultivars were 6.18~9.14 log CFU/g, and miso was the highest. Free amino acid and organic acid contents were highest in the jinpung and showed different composition depending on each sample. A total of 18 volatile aroma-compounds, including two acids, four alcohols, four ketones, three phenols, one furan, three pyrazines, and one miscellaneous compounds. In conclusion, it is expected that manufacturing A. oligze inoculation fermented soybean products using jinpung cultivar will improve quality.

The purpose of this study was to evaluate indoor air pollutants of children’s facilities in libraries. The indoor pollutants, which were managed under the “Indoor Air Quality Control Standards” and “Environmental Safety Standards for Children’s Activity Zones,” were measured within five months. The new environmental pollutants such as phthalates and pesticides were also measured. The pollutant-measuring device was installed in children’s spaces in libraries and children’s libraries of the metropols. The result of investigating indoor pollutants showed that the concentration of fungus and floating bacteria had low distributions due to the use of air purifiers in all libraries. The concentration of HCHO and TVOCs was also measured lower than the environmental standards in well-ventilated libraries. On the other hand, phthalates and pesticides were detected in all libraries. In the case of heavy metals, they were mainly found in the finishing materials of the library walls and floors. As a result, indoor pollutants are managed under court receivership. On the other hand, phthalates and pesticides, which are not regulated by environmental standards should be managed because they were detected in all libraries.

For the important safety system, two or more units of identical equipment or redundant components with similar function were installed to prevent abnormal failure. If the failure probability of such equipment is independent, this redundancy could increase the system safety remarkably. However, if the failure of each component is highly correlated by installing in a structure or experiencing an earthquake event, the expected redundancy effect will decrease. Therefore, the seismic correlation of the equipment should be evaluated quantitatively for the seismic probabilistic safety assessment. The correlation effect can be explained in the procedure of constructing fragility curves. In this study, several methodologies to quantify the seismic correlation in the failure probability calculation for multiple components were reviewed and two possible ways considering the realistic situation were selected. Simple examples were tested to check the applicability of these methods. The conversion method between these two methods was suggested to render the evaluation using the advantages of each method possible.

Analysis of the 2016 Gyeongju earthquake and the 2017 Pohang earthquake showed the characteristics of a typical high-frequency earthquake with many high-frequency components, short time strong motion duration, and large peak ground acceleration relative to the magnitude of the earthquake. Domestic nuclear power plants were designed and evaluated based on NRC's Regulatory Guide 1.60 design response spectrum, which had a great deal of energy in the low-frequency range. Therefore, nuclear power plants should carry out seismic verification and seismic performance evaluation of systems, structures, and components by reflecting the domestic characteristics of earthquakes. In this study, high-frequency amplification factors that can be used for seismic verification and seismic performance evaluation of nuclear power plant systems, structures, and equipment were analyzed. In order to analyze the high-frequency amplification factor, five sets of seismic time history were generated, which were matched with the uniform hazard response spectrum to reflect the characteristics of domestic earthquake motion. The nuclear power plant was subjected to seismic analysis for the construction of the Korean standard nuclear power plant, OPR1000, which is a reactor building, an auxiliary building assembly, a component cooling water heat exchanger building, and an essential service water building. Based on the results of the seismic analysis, a high-frequency amplification factor was derived upon the calculation of the floor response spectrum of the important locations of nuclear power plants. The high-frequency amplification factor can be effectively used for the seismic verification and seismic performance evaluation of electric equipment which are sensitive to high-frequency earthquakes.

In this study, we develop a sampling-based seismic probabilistic risk assessment (SPRA) quantification technique that can accurately consider a partially dependent condition of component seismic fragility information. Specifically, the SPRA quantification method is proposed by combining the advantages of two representative methodologies: EPRI seismic fragility and JAERI seismic fragility input-based quantification. The most important feature of the proposed method is that it performs a SPRA using a sampling technique by transforming the EPRI seismic fragility input into JAERI seismic fragility input. When the proposed sampling-based approach was applied to an example of simple system and to a SPRA problem of a nuclear power plant, it was observed that the proposed method yields approximately similar system seismic fragility and seismic risk results as those of the exact solution. Therefore, it is believed that the approach proposed in this study can be used as a useful tool for accurately assessing seismic risks, considering the partial seismic dependence among the components; the existing SPRA method cannot handle such partial dependencies.

Seismic responses due to the dynamic coupling between a primary structure and secondary system connected to a structure are analyzed in this study. The seismic responses are compared based on dynamic coupling criteria and according to the error level in the natural frequency, with the recent criteria being reliant on the error level in the spectral displacement response. The acceleration responses and relative displacement responses of a primary structure and a secondary system for a coupled model and two different decoupled models of two degrees-of-freedom system are calculated by means of the time integration method. Errors in seismic responses of the uncoupled models are reduced with the recent criteria. As the natural frequency of the secondary system increases, error in the natural frequency decreases, but seismic responses of uncoupled models can be underestimated compared to that of coupled model. Results in this paper can help determine dynamic coupling and predict uncoupled models’ response conservatism.

In 2016, an earthquake occurred at Gyeongju, Korea. At the Wolsong site, the observed peak ground acceleration was lower than the operating basis earthquake (OBE) level of Wolsong nuclear power plant. However, the measured spectral acceleration value exceeded the spectral acceleration of the operating-basis earthquake (OBE) level in some sections of the response spectrum, resulting in a manual shutdown of the nuclear power plant. Analysis of the response spectra shape of the Gyeongju earthquake motion showed that the high-frequency components are stronger than the response spectra shape used in nuclear power plant design. Therefore, the seismic performance evaluation of structures and equipment of nuclear power plants should be made to reflect the characteristics of site-specific earthquakes. In general, the floor response spectrum shape at the installation site or the generalized response spectrum shape is used for the seismic performance evaluation of structures and equipment. In this study, a generalized response spectrum shape is proposed for seismic performance evaluation of structures and equipment for nuclear power plants. The proposed response spectrum shape reflects the characteristics of earthquake motion in Korea through earthquake hazard analysis, and it can be applied to structures and equipment at various locations.

본 연구에서는 바이오매스 폐기물인 Corynebacterium glutamium을 Alg를 이용한 고정화와 PEI 표면개질 과정을 통하여 유해 미세조류인 Microcystis aeruginosa를 제거할 수 있는 흡착소재인 PEI-AlgBF를 개발하였다. 녹조의 발생단계에 상관없이 PEI-AlgBF는 수계로부터 M. aeruginosa를 성공적으로 제거할 수 있었으며 유해조류 제거과정에서 M. aeruginosa 세포의 파괴를 유발하지 않았다. 흡착소재의 표면적은 M. aeruginosa의 제거효율에 매우 큰 영향을 주는 주요인자로 확인할 수 있었다. PEI-AlgBF를 사용한 M. aeruginosa 흡착/제거 방식은 기존 기술에 비하여 환경영 향성이 낮기 때문에 보다 안전하고 안정적인 유해조류의 제어 방식이 될 것이다.