A liquid chromatographic method, using matrix solid-phase dispersion (MSPD) is developed for the extraction of residual furazolidone in chicken eggs. Blank or fortified egg samples (0.5 g) were blended with Octadecylsilyl (Bulk C,,, 40 pm, 18%. load, endcapped. 2 g) derivatized silica. After homogenization, C,,/egg and Na2S0, matrix were transferred to a column made of 10 ml glass synnge and filter paper and compressed 4.0-4.5 ml volume. The column was washed with 8 ml of hexane and dried under N, gas. Furazolidone was eluted with acetonitrile (8 ml) under gravity. The eluate containing furazolidone was free from interfering compounds when analyzed by HPLC with UV detection (365 nm, photodiode array). Calibration curves were linear (r = 0.99985) and inter- (1.47%) and intra-assay (5.29%) variabilities for the concentration range examined (7.8-497 ngig of eggs, 20 pl injection volume) were indicative of an acceptable methodology for the analysis of furazolidone. Average recovery of furazolidone added to egg was 96.2%. The limit of detection for the proposed method was 1 ng/g for furazolidone. The method using MSPD is proposed as an alternative assay to the classical method which involves the use of large volumes of a harmful solvent and requires a long tedious separation and clean-up processes prior to its determination.

In this study, we investigated the anti oxidative potential and protective effects of water extract of Leonurus sibiricus L. leaf (LSLW) against ultraviolet B (UVB)-induced oxidative damage in human keratinocytes (HaCaT cells). To evaluate the anti oxidative activity of LSLW, we measured DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion scavenging activities, lipid peroxidation inhibition, and reducing power of LSLW. For induction of oxidative stress in HaCaT cells, the cells were irradiated with UVB at 40 mJ/㎠. To investigate the protective effects of LSLW against UVB, we measured cell viability and apoptotic bodies using annexin V staining. LSLW showed anti oxidative activities by scavenging DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion and by reducing lipid peroxidation. In addition, LSLW showed high reducing values. The UVB-induced oxidative conditions led to cell apoptosis. However, treatment with LSLW ameliorated oxidative stress conditions, including inhibition of cell death, apoptotic body. Taken together, LSLW exhibited anti oxidative and protective effects against UVB-induced damage in HaCaT cells. Thus, LSLW could be useful for the development of cosmetics for UVB-induced skin aging.

This study examines the management performance of Oilseed crops (Sesame and Perilla). For this purpose, In the first stage, This study analyzes the current status of sesame and perilla industry. In the second stage, This study examines the management performance of environmental friendly agricultural products (Sesame and perilla). The result of this study show that : (1) Changes in annual wholesale price of Sesame and Perilla ; (2) Management performance of environmental friendly products (Sesame and perilla) ; (3) Feature comparison of productivity of oilseed crops.

This study evaluated compressive strength on the slurry for HPFRCC using blast- furnace slag with various blain as a basic study for developing alternative technology of the existing HPFRCC.

A kinetic study for nitrate removal by anion exchange resin was performed using continuous column reactors. Kinetic approach from the packed bed showed the reaction rate constant k1 was 0.07∼0.17 ℓ/㎎·hr and maximum exchange quantity q0 was 27.75∼31.81 ㎎/g. The results from the continuous column well agreed with that from the batch reactor. An economic analysis of the water treatment plant by anion exchange resin with a regenerating system was performed to design plant and process. Based on the treatment of 20 ㎎/ℓ nitrate-contained wastewater of 10,000 gallons per day to 2 ㎎/ℓ , total capital cost and total annual cost are estimated to be 836 million wons and 211 million wons, respectively.