The aim of the present study was to investigate the differences in volatile profiles among different Korean chrysanthemum cultivars grown as cut flowers. To optimize a method for sampling and comparing scents, the scents were evaluated by electronic nose (E-nose) at different flowering stages and in different organs and cultivars. The values of maximum resistance changes of metal oxide semiconductor sensors and relative aroma intensity were highest at flowering stage III (full flower stage of ray florets and initial opening disc florets) among different stages and in disc florets among different floral organs of cut chrysanthemums. Among the 12 chrysanthemum cultivars, the highest values for response change and relative aroma intensity were observed in the flowers of the ‘Ilweol’ cultivar. To compare scent patterns among the cultivars, E-nose sensor data was subjected to multivariate statistical analysis. Principal component analysis and discriminant function analysis of the volatile metabolic profile data indicated that chrysanthemum samples were clearly discriminated in a cultivar-dependent manner. These results show that different cultivars are characterized by distinct volatile profiles. Hierarchical cluster analysis showed that the 12 chrysanthemum cultivars were separated into 3 main groups according to the relationship of volatile profiles; however, chrysanthemum cultivars were not clustered according to the flower shape. We propose that these results could be used as the basis on which to improve the breeding of cut chrysanthemums based on volatile characteristics.

본 연구는 몇가지 전처리제 및 보존용액 처리가 국내 육성 절화 프리지아 ‘Gold Rich’의 수확후 절화수명과 개화 품질에 미치는 영향을 구명하고자 수행하였다. 10% sucrose, 200mg･L-1 Al2(SO4)3, 200mg･L-1 GA3, 150mg･L-1 citric acid가 혼용된 전처리 용액에서 절화 관상 기간 동안 수분 흡수량과 생체중은 유의적으로 증가한 것으로 조사되었지만 절화수명에는 큰 영향을 미치지 않았다. 보존용액으로 5% sucrose와 살균제인 300mg･L-1 8-HQ를 혼용 처리하였을 때 절화수명 연장(1일 이상), 높은 개화율(82.3~89.1%) 및 만개율(71.5~84.2%) 등의 개화 품질이 향상되었다. 이러한 결과들을 통해 10% sucrose, 200mg･L-1 Al2(SO4)3, 200mg･L-1 GA3, 150mg･L-1 citric acid를 혼합한 전처리제와 5% sucrose, 300mg･L-1 8-HQ를 혼합한 보존용액 처리는 절화수명 연장, 개화율 및 만개율 증가에 효과적일 것이라고 판단된다. 특히, sucrose가 포함된 보존용액은 절화 프리지아 ‘Gold Rich’ 의 상품성 향상에 있어 매우 유용할 것이다.

국립원예특작과학원에서는 2015년 거베라 신품종 ‘Red Velvet’ 을 육성하였다. ‘Red Velvet’은 2007년 10월부터 2008년 2월까지 주홍색 반겹꽃 스탠다드 거베라 ‘Rose’와 적색 반겹꽃 ‘Sabana’를 각각 모본과 부본으로 인공교배하여 얻은 187개체의 교배실생 분리집단으로부터 2008년부터 2015년까지 8년 동안의 개화 및 생육특성검정을 거쳐 선발된 절화용 스탠다드 거베라 품종이다. ‘Red Velvet’은 초세 강건한 갈색 화심의 적색(RHS R45B) 반겹꽃 대륜계통이다. 꽃의 평균 직경은 12.6±0.5cm이고, 내부설상화 및 통상화의 직경은 각각 5.7±0.3cm와 2.8±0.2cm이다. 꽃대는 꽃목직경이 상부 5.4±0.4mm, 중부 7.8±0.7mm 정도로 굵으며, 화경장은 60.0±8.1cm로 길다. 절화수명은 9.8±2.3일로 대조품종과 비슷하며, 연간 채화량은 48.3±4.8본/주로 많은 다수성 품종이다.

‘Field Green’은 농촌진흥청 국립원예특작과학원에서 2012년에 개발한 국화 신품종이다. 2008년 황색 홑꽃인 ‘Laque Roicks’를 모본으로 하고 흰녹병저항성이며 연두색 홑꽃인 ‘Frill Green’을 부본으로 인공교배를 실시하였다. 실생개체중에서 녹색이며 잘 자라고 꽃모양이 안정적인 개체를 2009년에 선발하였다. 그리고 2010년부터 2012년까지 3년동안 촉성재배와 억제재배, 자연재배을 통해 특성검정을 실시하고 최종 선발을 하였다. ‘Field Green’ 은 자연일장조건에서 10월 26일에 개화하는 품종으로 일장조절을 통해 연중재배가 가능하였다. ‘Field Green’ 품종은 밝은 녹색의 설상화와 녹색의 통상화를 가진 홑꽃이다. 한 꽃당 설상화수는 18.8매이고 꽃의 크기는 7.1cm이었으며 초장은 130.0cm으로 줄기생장이 우수하였다. 그리고 흰녹병에 저항성이고 절화수명은 20.0일 정도였다. ‘Field Green’은 2013년 2월 20일 품종보호출원(출원번호: 2013-153)하고 국립종자원에 2014년 4월 2일 품종등록번호 제4839호로 품종보호등록을 완료하였다.

Because of their attractive and colorful flowers, many species from the genus Aster serve as garden plants. Chrysanthemum owes its popularity to its ornamental and medicinal herb value. It can be used as a cut flower, potted plant, vegetable, and herbal tea. Plant breeders have attempted to identify the available species and produce new cultivars to improve the quality of chrysanthemum for commercial purposes. The use of cytogenetic studies has paved the way for identifying compatibility, ancestry, and other useful information for this undertaking. Thus, an investigation was conducted into the chromosome numbers of 23 wild Asteraceae species in Republic of Korea to determine their genetic characteristics and variations. The somatic chromosome spread has been used for chromosome counting. The results revealed that Asteraceae species have a chromosome range from 18 (diploid) to 54 (hexaploid). These findings provide primary and important information on the chromosome numbers in chrysanthemum plants that can be used to select the right variety for cultivation.

Chrysanthemum is a valuable ornamental plant worldwide, and several of its species are used as herbal tea, medicinal plants, and dietary supplements, among others. Commercial cultivars have been developed through interspecific hybridization and artificial selection to improve the characteristics, production quality, and environmental adaptation for enhancing ornamental value. To better understand the recent research in cytogenetic studies of chrysanthemum, we examined studies concerning polyploidy, karyotyping, banding, fluorescence in situ hybridization (FISH) technique, and inter/intraspecific hybridization. Ploidy level is important in genomic characteristics and has a significant value to horticulturists and plant breeders. Studies have reported that flow cytometry analysis and single-dose molecular markers can be used to determine the chrysanthemum ploidy level. As for karyotyping, a better understanding of karyomorphological relationships and evolution of chrysanthemum and its closely related genera has already been gained. Moreover, karyotype parameters in chrysanthemum studies play a critical role in cultivar identification, classification, and genetic analysis. The FISH technique in chrysanthemum research provides more information on chromosome identification, sequences distribution, and evolution for expediting the development and improvement of plant species. The genomic in situ hybridization (GISH) technique can also be used to test hybridization in chrysanthemum breeding. Hence, this review of molecular cytogenetic studies of chrysanthemum will help us to have a better understanding and knowledge of the taxa breeding and the development and improvement of new cultivars.

Despite growing research interest in consumer health, well-being and beauty, little is known about factors influencing consumer purchase decisions for female fitness devices. This study focuses on Kegel exercises – exercise routines developed by Dr. Kegel to strengthen pelvic floor muscles – that are recommended by health professionals for patients experiencing health ailments such as urinary incontinence, sexual dysfunction, and dissatisfaction. Despite the benefits, women are not well aware of the role of pelvic floor muscles. The diffusion and acceptance of Kegel exercise and devices among women are likely determined by how comfortable women are about obtaining such benefits. For this reason, feminism should play an important role in shaping women’s desire to engage in Kegel exercises. Thus, this research investigates how feminism is related to Millennial women’s engagement with fitness products, particularly Kegel devices. Drawing on the literatures about sexuality, health, and marketing, we propose that feminism positively influences women’s health-related attitudes (i.e., attitudes towards fitness, fitness opinion leadership, attitude toward core strength, and desire to control sex life), which in turn positively impacts women’s fitness engagement. Fitness engagement, in turn, is expected to predict attitudes towards Kegel exercise and Kegel device purchase intentions. On the other hand, women’s attitudes toward core strength and desire to control sex life are proposed to have a direct, positive impact on attitude towards Kegel exercise. Analysis of PLS-SEM shows general support for the hypotheses. We discuss how interest in Kegel devices could, in turn, lead to positive effects on overall physical and psychological health and provide implications for marketing practitioners.

Feeding effects of the honeybee pollen products from both domestic, China, Spain and mixture of different origin on the colony development of earth bumblebee, Bombus terrestris L., were surveyed to evaluate efficient nutritional resources for commercial bombiculture in Korea. As the results, the domestic pollen was most effective to achieve high rates of oviposition (88%), colony foundation (70%), and queen production. While feeding with domestic pollens during the egg-laying period, and domestic+Chinese mixture (5:2) during the breeding period (Plot-2), it revealed high oviposition rate of 75%, colony foundation of 65%, and large numbers of adult queen production, indicating its suitability for generation subculturing. In the Plot-3, the same high oviposition rate (75%) was obtained except for feeding with the domestic+Chinese mixture (2:5) during the breeding period, which produced large number of workers.

2017년 한해 동안 9개 공항만에서 중국 등 32개국, 120개 품목 8,660건을 조사한 결과, 523건에서 해충 검출이 있었다. 반입빈도가 높은 품목은 사과, 망고, 감귤, 배, 복숭아, 바나나, 오렌지, 오이, 고추. 호두(미탈각), 자두, 드래곤프릇, 포멜로, 토마토, 풋콩, 망고스틴, 대추 등의 순이었으며, 중국, 베트남, 일본, 필리핀, 대만, 태국 등의 나라에서 반입건수가 많았다. 해충 검출 빈도가 높은 품목은 망고스틴, 망고, 사과, 슈가애플, 람부탄, 고추, 자두, 구아바 등이며, 해충 검출 빈도가 높은 국가는 중국, 베트남, 태국 등이었다. 검출 해충류 중 금지해충으로 오리엔탈과실파리류(Bactrocera dorsalis sp. complex) 52건, 오이과실파리(Bactrocera cucurbitae) 1건, Bactrocera latifrons 2건이 있었으며, 과실파리류 32건이 동정중이며, 코드린나방(Cydia pomonella)이 1건 검출되었다.

Earthworms, especially Eisenia andrei, are cultivated for a variety of purposes including waste disposal and compost production. In this study, bacteria from earthworm gut were cultured and the dominant species identified. Subsequently, we isolated bacteriophages able to lyse the isolated gut bacteria. Two dominant genera of gut bacteria, Aeromonas sp. and Citrobacter sp. were identified by using MALDI-TOF MS analysis and a library was constructed to find lytic phages. Phage EF1 showed lytic activity for C. freundii and two Citrobacter isolates, C. braakii and C. murliniae. These 3 species have similar sensitivities to EF1. Several aspects of the life cycle of EF1 were investigated by using C. freundii under optimal growth conditions. EF1 infects C. freundii with a moderate latent period, approximately 25 min, and a large burst size averaging 5 × 109 per infected cell. Moreover, the antimicrobial activity of EF1 was well maintained under diverse conditions including a broad temperature range of 40°C to 50°C and a wide pH range of 4 to 11. In conclusion, the results indicate that earthworm casting contain a wide range of bacteria species, for which there are various corresponding bacteriophages.

This study investigated the antidiabetic effect of amaranth grain ethanol extract (AEE) in a diabetic animal model, db/db mouse. The mice were divided into 4 groups: normal control mice (C57BL/6J), diabetic mice (C57BL/6J db/db), diabetic mice fed a lower concentration of AEE (0.3 mg/kg), and diabetic mice fed a higher concentration of AEE (0.5 mg/kg). After 10 weeks of treatment, body weights, blood insulin levels and blood glucose levels of each group were compared. At the end of treatment, the results showed that both AEE supplemented groups had lower body weights than those in the diabetic groups although higher than those in the normal groups. Moreover, in both AEE supplemented groups, serum insulin levels were higher and blood glucose levels were lower than those in the diabetic groups although both values were higher than those in the normal groups. The results of this study suggest that AEE can alleviate many of the common symptoms of diabetes in diabetic mice and, therefore, has potential as a therapeutic supplement for normalization of blood glucose and insulin levels in humans.

‘Woonbaek’ bred by the National Institute of Horticultural & Herbal Science, Rural Development Administration (RDA) in 2011 is a new white standard chrysanthemum cultivar. The artificial crossing was made in 2007 between ‘Suishin’ and ‘Gilyangma’. ‘Suishin’ was the autumn-flowering standard chrysanthemum cultivar with white petals and low temperature flowering type, while ‘Gilyangma‘ grew well at low temperature. Vigorous plants with numerous white petals and low branches were selected in 2008. Genetic trait investigations of the line (07B1-659) had been performed between 2009 and 2011 and included flowering stability, uniformity, and year-round productivity, the final test of the producer and consumer preference. ‘Woonbaek’ is a new standard cultivar that blooms in late October in natural condition. The flower has numerous petals, a green colored center, and a s table flower shape. The number of ray flower was 249 and flower diameter was 14 cm. Vase life was approximately 21 days in autumn season. The stem was not significantly thinner at the upper end. ‘Woonbaek’ was applied as No. 2012 - 244 on March 23, 2012, for variety protection and its plant variety protection rights have been registered as No. 4839 on March 17, 2014, at the Korea Seed and Variety Service.

Mimosa pudica (미모사), Argemone mexicana (멕시코 가시양귀비) Leucus aspara (꿀풀과 일종), Polygonum hydropiper (여뀌), Blumea lacera (국화과 일종) 등 5종의 식물 헥산추출물들의 팥바구미 성충에 대한 살충, 성충우화억제력 및 녹두 종실피해 방제력이 검증되었다. 그 결과, 밭바구미 성충 살충력은 35-69%이었으며, 녹두에 추출물을 처리한 후 성충우화 방제율은 33-63%, 종실 피해 방제율은 13-49%이었다. 추출물의 농도가 증가할수록 살충력, 성충우화 및 종실피해 방제율 등이 증가하는 것으로 나타났다. 이와 같이 5종의 식물 헥산추출물들은 밭바구미 친환경 방제제로 이용가능성이 있을 것으로 판단된다.

본 연구는 광중단 처리에 있어서 광원 및 광질이 스탠다드 국화의 생육 및 개화에 미치는 영향을 알아보기 위해 수행하였다. 스탠다드 국화 ‘백마’, ‘신마’ 품종에 LED 590, 610, 630, 660, 680nm와 형광등 480+540 +610nm의 혼합광을 정식 후부터 단일처리일 전까지 40 일간 1일 4시간(22-02시) 광중단 처리하였다. ‘백마’의 발뢰소요일수는 형광등 처리에서 21.3일로 가장 길었으며, LED 590nm 처리는 15.8일로 가장 짧았다. ‘신마’는 형광등, LED 610nm와 660nm 처리에서 발뢰소요일수가 각각 18.0일, 17.8일, 17.7일로 가장 길었으며, LED 590nm 처리에서 15.1일로 가장 짧았다. ‘백마’의 개화소 요일수는 형광등처리에서 56.9일로 가장 길었고, LED 590nm 처리에서 51.6일로 가장 짧았다. ‘신마’는 형광등과 LED 660nm 처리에서 각각 56.0일, 56.7일로 가장 길었고, LED 590nm 처리에서 52.9일로 가장 짧았다. 따라서, 스탠다드 국화의 화아분화 및 개화조절에 가장 효과적인 광원 및 광질은 ‘백마’ 품종의 경우 형광등 처리가 가장 효과적이며, ‘신마’ 품종은 형광등과 LED 660nm 처리가 가장 효과적이라고 생각된다. ‘백마’ 품종 의 절화장과 절화중은 화아분화 억제효과가 가장 좋았던 형광등 처리에서 각각 92.7cm, 61.9g으로 우수하였으며, ‘신마’ 품종의 경우에도 형광등 처리에서 절화장, 절화중이 각각 114.6cm, 71.3g으로 우수하였다. 결과적으로 스탠다드 국화의 생육 및 개화에 있어서 광중단 광원 및 광질로 형광등 처리가 우수하였으며, LED 660nm 처리도 효과가 있는 것으로 생각된다.