Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We report 97 mitochondrial genome (mitogenome) sequences of B. mandarina collected from Korea and Japan. Phylogenetic and population genetic analyses showed that all individuals of B. mandarina collected in Korean localities formed a strong group together with all individuals originated from northern China (mainly north of the Qinling-Huaihe line) and some of southern China. This group was placed as the sister group to B. mori strians suggesting that this group had been served as an immediate progenitor for B. mori.

해충에 이용되는 화학적 기피제는 생태계를 파괴할 수 있으며 내성을 가진 생물체로의 진화를 촉진한다. 같은 종의 생물끼리의 의사소통 수단인 페로몬을 이용하면 다른 종에게 영향을 미치지 않으면서 특정 곤충에 특이적 으로 작용하는 방충제를 제작할 수 있을 것이라 생각된다. 본 연구는 초파리(Drosophila)의 페로몬 2종류를 추출 하여 초파리의 기피도 및 유인도와 번식률을 확인하고자 한다. ℃, 광주기 12h/12h의 동일한 조건에서 사육하 며 10마리당 헥세인 10를 사용하여 암컷의 표피에서 CHC 페로몬과 수컷의 페로몬샘에서 cVA 페로몬을 추출 한다. 연령, 성별, 교배 여부에 따라 관찰통에 각각의 페로몬을 처리하여 지정구간에 분포하는 초파리의 수를 계수하여 기피도 및 유인도를 확인한다. 관병에 암수 1쌍을 투입하고 하루에 1번 선정한 페로몬을 투여하며 산란 수을 측정한다. 이 연구를 통해 CHC가 수컷 초파리에 대한 기피 효과가 있음을 확인하였으며 추출되는 수컷의 연령이 높을수록 cVA에 의한 번식률 감소가 크게 나타났다. 본 연구를 통해 페로몬을 통한 초파리의 방제 가능성 을 확인하였으므로 다른 곤충의 방제에도 적용할 수 있을 거라 기대한다. 페로몬은 생물 농축과 같은 환경적 영향이 없으며 소량으로 유의미한 결과를 도출했다는 점에서 의의가 있으며 상용화를 통해 해충에 의해 피해를 해결할 수 있을 것이라 기대한다.

This study was aimed to isolate bacterial inoculants producing chitinase and evaluate their application effects on corn silage. Four corn silages were collected from four beef cattle farms to serve as the sources of bacterial inoculants. All isolates were tested against Fusarium graminearum head blight fungus MHGNU F132 to confirm their antifungal effects. The enzyme activities (carboxylesterase and chitinase) were also measured to isolate the bacterial inoculant. Based on the activities of anti-head blight fungus, carboxylesterase, and chitinase, L. buchneri L11-1 and L. paracasei L9-3 were subjected to silage production. Corn forage (cv. Gwangpyeongok) was ensiled into a 10 L mini silo (5 kg) in quadruplication for 90 days. A 2 × 2 factorial design consists of F. graminearum contamination at 1.0104 cfu/g (UCT (no contamination) vs. CT (contamination)) and inoculant application at 2.1 × 105 cfu/g (CON (no inoculant) vs. INO (inoculant)) used in this study. After 90 days of ensiling, the contents of CP, NDF, and ADF increased (p<0.05) by F. graminearum contamination, while IVDMD, acetate, and aerobic stability decreased (p<0.05). Meanwhile, aerobic stability decreased (p<0.05) by inoculant application. There were interaction effects (p<0.05) on IVNDFD, NH3-N, LAB, and yeast, which were highest in UCT-INO, UCT-CON, CT-INO, and CT-CON & INO, respectively. In conclusion, this study found that mold contamination could negatively impact silage quality, but isolated inoculants had limited effects on IVNDFD and yeast.

Compared to operational wastes, nuclear power plant (NPP) decommissioning wastes are generated in larger quantities within a short time and include diverse types with a wider range of radiation characteristics. Currently used 200 L drums and IP-2 type transport containers are inefficient and restrictive in packaging and transporting decommissioning wastes. Therefore, new packaging and transport containers with greater size, loading weight, and shielding performance have been developed. When transporting radioactive materials, radiological safety should be assessed by reflecting parameters such as the type and quantity of the package, transport route, and transport environment. Thus far, safety evaluations of radioactive waste transport have mainly targeted operational wastes, that have less radioactivity and a smaller amount per transport than decommissioning wastes. Therefore, in this study, the possible radiation effects during the transport from NPP to disposal facilities were evaluated to reflect the characteristics of the newly developed containers and decommissioning wastes. According to the evaluation results, the exposure dose to transport workers, handling workers, and the public was lower than the domestic regulatory limit. In addition, all exposure dose results were confirmed, through sensitivity analysis, to satisfy the evaluation criteria even under circumstances when radioactive materials were released 100% from the container.

As nuclear power plants are operated in Korea, low and intermediate-level radioactive wastes and spent nuclear fuels are continuously generated. Due to the increase in the amount of radioactive waste generated, the demand for transportation of radioactive wastes in Korea is increasing. This can have radiological effect for public and worker, risk assessment for radioactive waste transportation should be preceded. Especially, if the radionuclides release in the ocean because of ship sinking accident, it can cause internal exposure by ingestion of aquatic foods. Thus, it is necessary to analyze process of internal exposure due to ingestion. The object of this study is to analyze internal exposure by ingestion of aquatic foods. In this study, we analyzed the process and the evaluation methodology of internal exposure caused by aquatic foods ingestion in MARINRAD, a risk assessment code for marine transport sinking accidents developed by the Sandia National Laboratory (SNL). To calculate the ingestion internal exposure dose, the ingestion concentrations of radionuclides caused by the food chain are calculated first. For this purpose, MARINRAD divide the food chain into three stages; prey, primary predator, and secondary predator. Marine species in each food chain are not specific but general to accommodate a wide variety of global consumer groups. The ingestion concentrations of radionuclides are expressed as an ingestion concentration factors. In the case of prey, the ingestion concentration factors apply the value derived from biological experiments. The predator's ingestion concentration factors are calculated by considering factors such as fraction of nuclide absorbed in gut, ingestion rate, etc. When calculating the ingestion internal exposure dose, the previously calculated ingestion concentration factor, consumption of aquatic food, and dose conversion factor for ingestion are considered. MARINRAD assume that humans consume all marine species presented in the food chain. Marine species consumption is assumed approximate and conservative values for generality. In the internal exposure evaluation by aquatic foods ingestion in this study, the ingestion concetration factor considering the food chain, the fraction of nuclide absorbed in predator’s gut, ingestion rate of predator, etc. were considered as influencing factors. In order to evaluate the risk of maritime transportation reflecting domestic characteristics, factors such as domestic food chains and ingestion rate should be considered. The result of this study can be used as basis for risk assessment for maritime transportation in Korea.

Kori unit 1, the first PWR (Pressurized Water Reactor) in Korea, was permanent shut down in 2017. In Korea, according to the Nuclear Safety Act, the FDP (Final Decommissioning Plan) must be submitted within 5 years of permanent shutdown. According to NSSC Notice, the types, volumes, and radioactivity of solid radioactive wastes should be included in FDP chapter 9, Radioactive Waste Management, Therefore, in this study, the types depending on generation characteristics and radiological characterization methods and process of solid radioactive waste were analyzed. Solid radioactive waste depending on the characteristics of the generation was classified into reactor vessel and reactor vessel internal, large components, small metals, spent nuclear fuel storage racks, insulation, wires, concrete debris, scattering concrete, asbestos, mixed waste, soil, spent resins and filters, and dry active waste. Radiological characterization of solid radioactive waste is performed to determine the characteristics of radioactive contamination, including the type and concentration of radionuclides. It is necessary to ensure the representativeness of the sample for the structures, systems and components to be evaluated and to apply appropriate evaluation methods and procedures according to the structure, material and type of contamination. Therefore, the radiological characterization is divided into concrete and structures, systems and components, and reactor vessel, reactor vessel internal and bioshield concrete. In this study, the types depending on generation characteristics and radiological characterization methods and process of solid radioactive waste were analyzed. The results of this study can be used as a basis for the preparation of the FDP for the Kori unit 1.

Wolsong unit 1, the first PHWR (Pressurized Heavy Water Reactor) in Korea, was permanent shut down in 2019. In Korea, according to the Nuclear Safety Act, the FDP (Final Decommissioning Plan) must be submitted within 5 years of permanent shutdown. According to NSSC Notice, the types, volumes, and radioactivity of solid radioactive wastes should be included in FDP chapter 9, Radioactive Waste Management, Therefore, in this study, activation assessment and waste classification of the End shield, which is a major activation component, were conducted. MCNP and ORIGEN-S computer codes were used for the activation assessment of the End shield. Radioactive waste levels were classified according to the cooling period of 0 to 20 years in consideration of the actual start of decommissioning. The End shield consists of Lattice tube, Shielding ball, Sleeve insert, Calandria tube shielding sleeve, and Embedment Ring. Among the components composed for each fuel channel, the neutron flux was calculated for the components whose level was not predicted by preliminary activation assessment, by dividing them into three channel regions: central channel, inter channel, and outer channel. In the case of the shielding ball, the neutron flux was calculated in the area up to 10 cm close to the core and other parts to check the decrease in neutron flux with the distance from the core. The neutron flux calculations showed that the highest neutron flux was calculated at the Sleeve insert, the component closest to the fuel channel. It was found that the neutron flux decreased by about 1/10 to 1/20 as the distance from the core increased by 20 cm. The outer channel was found to have about 30% of the neutron flux of the center channel. It was found that no change in radioactive waste level due to decay occurred during the 0 to 20 years cooling period. In this study, activation assessment and waste classification of End Shield in Wolsong unit 1 was conducted. The results of this study can be used as a basis for the preparation of the FDP for the Wolsong unit 1.

In Korea, the construction of dry storage facilities for spent nuclear fuel is being promoted through the 2nd basic plan for high-level radioactive waste management. When operating dry storage facilities, exposure dose assessment for workers should be performed, and for this, exposure scenarios based on work procedures should be derived prior. However, the dry storage method has not yet been sufficiently established in Korea, so the work procedure has not been established. Therefore, research is needed to apply it domestically based on the analysis of spent nuclear fuel management methods in major overseas leading countries. In this study, the procedure for receiving and storing spent nuclear fuel in a concrete overpack-based storage facility was analyzed. Among the various spent nuclear fuel management systems, the metal overpack-based HI-STAR 100 system and the concrete overpackbased HI-STORM 100 system are quite common methods in the United States. Therefore, in this study, work procedures were analyzed based on each final safety analysis report. First, the HI-STAR 100 overpack enters the facility and is placed in the transfer area. Remove the impact limiter of the overpack and install the alignment device on the top of the overpack. Place the HI-TRAC, an on-site transfer device, on top of the alignment unit and remove the lids of the two devices to insert the canister into the HI-TRAC. When the canister transfer is complete, reseat the lid to seal it, and disconnect the HI-TRAC from the HI-STAR 100. Raise the canister-loaded HI-TRAC over the alignment device on the top of the HI-STORM 100 overpack and remove the lids of the two devices that are in contact. Insert the canister into the HI-STORM 100 and reseat the lid. The HI-STORM 100 loaded with spent nuclear fuel is transferred to the designated storage area. In this study, the procedure for receiving and storing spent nuclear fuel in a concrete overpack-based storage facility was analyzed. The main procedure was the transfer of canisters between overpacks, and it was confirmed that HI-TRAC was used in the work procedure. The results of this study can be used as basic data for evaluating the exposure dose of operating workers for the construction of dry storage facilities in Korea.

본 연구에서는 칡의 효율적인 제거 방법을 모색하기 위해 국내외의 사례를 비교하였다. 칡은 척박한 토지에서도 생장이 왕성한 특성을 가져 사면침식을 억제하는 식물로 이용되기도 하였다. 그러나 칡은 덩굴을 형성하여 조림목을 감고 올라가 생장하므로 심할 경우에는 조림목의 고사를 유발한다. 최근에는 생활권 수목에 대한 관심의 증가로 조경수에 대한 피해가 주목을 받고 있다. 칡은 물리적, 화학적 및 생물학적 방법으로 제거한다. 국내에서는 덩굴 및 주두부 제거 등 물리적 제거 방법과 제초제를 사용하는 화학적 방법을 주로 사용하고 국외에서는 화학적 방법에 주로 의존한다. 물리적 방법은 환경오염이 적은 이점이 있으나 상당한 노동력과 비용이 소요된다. 화학적 방법은 즉각적으로 제거 효과가 나타나지만 약제 사용에 의한 환경오염과 기상조건의 제약이 있다. 생물학적 방법은 화학적 방법의 환경오염을 해결하기 위해 개발되었으나 제거 효과가 느리게 나타나고 조림지에 적용 시 조림목과 하층식생에 미치는 영향에 대한 우려가 있다. 향후 고효율성을 담보하며 친환경적인 생력화 칡 제거 기술에 대한 연구와 적용이 필요하다.

Currently, low and intermediate-level radioactive wastes and spent nuclear fuels are continuously generated in Korea. For the disposal of the radioactive wastes, the transport demand is expected to increase. Prior to transportation, it is necessary to evaluate the radiation risk of transportation to confirm that is not high. In Korea, there is no transportation risk assessment code that reflects domestic characteristics. Therefore, foreign assessment codes are used. In this study, before developing the overland transportation risk assessment code that reflects domestic characteristics, we analyzed the radiation risk assessment methodology in transportation accident codes developed in other countries. RADTRAN and RISKIND codes were selected as representative overland transportation risk assessment codes. For the two codes we analyzed accident scenarios, exposure pathways, and atmospheric diffusion. In RADTRAN, the user classifies accident severity for possible accident scenarios, and the user inputs the probability for each accident severity. On the other hand, in the case of RISKIND, the accident scenarios are classified and the probabilities are determined according to the NRC modal study (LLNL, 1987) in consideration of the cask impact velocity, cask impact angle, and fire temperature. In the case of RISKIND, the accident scenarios are applied only to transportation of spent nuclear fuel, and cannot be defined for low and intermediate-level radioactive waste. However, in the case of RADTRAN, since the severity and probability of accidents are defined by user, it can be applied to low and intermediate-level radioactive wastes. As the exposure pathways considered in transportation accident, both RADTRAN and RISKIND consider external exposure (cloudshine and groundshine), and internal exposure (inhalation, resuspension inhalation and ingestion). In the case of RADTRAN, additionally, external exposure due to loss of shielding (LOS) is considered. Atmospheric diffusion calculation is essential to determine the extent to which radioactive materials are diffused. In both RADTRAN and RISKIND, atmospheric diffusion calculations are based on Gaussian diffusion model. Users must input Pasquill stability class, release height, heat release, wind speed, temperature and mixing height, etc. Additionally, RADTRAN can input weather information relatively simply by inputting only the Pasquill stability class fraction and selecting the US average weather option. This study results will be used as a basis for developing radioactive waste overland transportation risk assessment code that reflects domestic characteristics.