This study was carried out to investigate insect community structure from different habitats in Baengnyeong island.We performed day and night collection at two different habitats (mountain, rural area) of Baengnyeong island from Mayto September in 2015. A total of 2,879 individuals of 404 species, 81 families belonging to 10 orders were collectedand identified. A dominant species was Idisia ornata Pascoe (Tenebrionidae) despite a very low percentage (6.04%) ofthe species among the catches. Results of independent t-test showed a significant high (p<0.001) of species richness onmountain. Also, seasonal results of ANOVA (Analysis of variance) were significantly influential with species abundanceand species richness. The result of NMDS analysis showed that the community structure of the insects from the mountainis different with rural area.

Among several insect pests in the potato fields, Liriomyza huidobrensis, pea leafminer, is emerging as a new threat to potato plants. Adult females damage the host mesophyll cells host due to ovipositor probing. After egg hatching, the eclosing larva also damage the leaf by mining the mesophyll and causing tissue death. Despite the importance of leafminer in potato production, there is no information available regarding its resistance level to potato cultivars in Korea. Using choice test, 21 potato cultivars consisting of 11 early-maturing, 6 mid-maturing and 4 late-maturing cultivars were evaluated the resistance to pea leafminer in the greenhouse and field condition. In this test, significant differences for resistance were observed. High levels of resistance to leafminers were obtained from cvs. Namsuh, Seohong, Jayoung and Superior. These resistant cultivars might be useful in future breeding programmes.

In the present study, Cydia kamijoi Oku is newly recognized in korean insect fauna. This tortricid moth was first found damaging the cones of Abies koreana in Jeju Island 2014. The moth can be a serious insect pest on A. koreana because of high damage rate on the cones, up to 71% average. The genus Cydia now was 11 korean species including C. kamijoi. Regarding this species, some basic information such as collection records, morphological characters, and ecology were provided

Insect pests on cruciferous crops and their natural enemies were surveyed during 2009~2011 in Daegwallyeong highland region which has been famous for summer Kimchi cabbage production in Korea since 1970s. Totally 15 insect pests have been reported in cabbage field in Daegwallyeong. Diamondback moth (DBM, Plutella xylostella) imported cabbage worm (Artogeia rapae L.), cabbage armyworm (CAW, Mamestra brassicae L.), cabbage looper (CL, Trichoplusia ni), cabbage sawfly (Athalia rosae ruficornis Jakovlev), and turnip aphid (Liphapis pseudobrassicae (Davis)) were the dominant pest species among the 15 species. For monitoring as well as controlling insects with weak flight, yellow sticky cards (10×15㎝) were used in cabbage fields. The sticky cards were hanged on plastic stick and placed at a 2-5 m distance within cabbage field (1,000㎡). Sex pheromone traps were employed for monitoring and controlling insects with strong flight. The survey result showed that there was significantly reduced pest occurrences in treated, compared to untreated condition. Similarly, The results from the sex pheromone traps were coincident with those of sticky trap. DBM, CAW and CL were found less in the treated than untreated fields; by 67.5%, 70.6% and 44.0%, respectively. Although the control efficacy of such traps was less than that of chemical spray, the use of sticky card trap combined with sex pheromone trap could be useful management tools for controlling insect pests in cabbage fields.

Pogonini Laporte, 1834 is one of the tribes in Trechinae Bonelli, 1810 (Coleoptera: Carabidae). This tribe is morphologically similar to Trechini, Benbidiini, Zolini and Patrobini of the subfamily. However, Pogonini can be distinguished from the other tribes by having a combination of the following characters: apical maxillary palpomere fusiform; umbilical series of setae on elytron more or less contiguous (though sparse in middle); each tarsomeres with a median sulcus on dorsal side. Pogonini includes about 85 species of 12 genera worldwide and is found in all zoogeographical regions of the world. Many species inhabit along the sea coasts or near salt lakes, and are recognized as halobiont. In Korea, Paik (1998) reported Pogonus (Pogonus) itoshimaensis Habu, 1954. However, he added only the species name in the checklist without description or illustration although few specimens were examined. After Paik’s study, any studies on taxonomic status and distribution of Korean Pogonini have not been carried out. The genus Pogonus Dejean is the largest group in Pogonini with two subgenera: P. (Pogonoidius Carret, 1903) and P. (Pogonus Dejean, 1821). These two subgenera share very similar external morphological characters; Pogonoidius has setae on anterior angles of pronotum and setae at 7th interval of elytra while Pogonus does not have them. In the present study, two species, Pogonus (Pogonus) japonicus Putzeys, 1875 and P. (Pogonoidius) punctulatus Dejean, 1828, are newly recognized from Korea. A key to the Korean Pogonini species, redescription, distribution, photos of diagnostic characters, and illustrations of male and female genitalia are provided.

Multiple starters consisting of two Bacillus amyloliquefaciens strains (MJ1-4 and EMD17), Pichiafarinosa SY80, and Rhizopus oryzae were used for Doenjang making. Bacillus strains were selected based on their abilities to inhibit toxinogenic fungi and Bacillus cereus, fibrinolytic activity, and their ability to confer good flavor to Cheonggukjang. P. farinosa SY80 and R. oryzae, previously isolated from soy sauce, were selected because they were not inhibited by two bacilli. Doenjang was prepared by inoculation of multiple starters (A1 Doenjang). Control Doenjang was prepared by inoculation of B. subtilis KACC 16750 (Natto strain) and Aspergillus oryzae KCCM 60166 (A2 Doenjang). Another control (A3 Doenjang) was prepared by inoculation of microorganisms present in rice straw. Doenjang samples were fermented for 70 days at 20℃. pH of 3 samples decreased from the initial value of 6.4 to 5.8~6.0 and titratable acidity (TA) increased from 0.6 to 1.1~1.3. The amount of amino-type nitrogen increased during fermentation. There were slight differences in moisture, crude-protein, and crude-fat contents after 70 days. Contamination of fungi was observed only in A3 Doenjang and B. cereus was not detected from all 3 samples. A1 Doenjang showed the highest fibrinolytic activity and A2 Doenjang the second. These results indicate that Doenjang made with carefully selected starters was functionally improved and microbially more safe.

Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of 4x105 pSSCs on mitotically in activated 2x105 STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

The present study is aimed to evaluate forest health in a fragmented urban forest using insects which are rarely used for evaluation of forest health. It is supposed that forest would be health if insect community in an urban forest is similar to that in healthy forests. Ants (Formicidae) and ground beetles (Carabidae) surveyed by pitfall trap method in the Hongneung forest located in the urban area of Seoul were compared with those in the Gwangneung forest. The Gwangneung forest is supposed to be health due to its high biodiversity and well-conserved nature. Ants of the Hongneung forest was much more abundant compared with those of the Gwangneung forest. However, ground beetles showed the opposite patterns; much more abundant in the Gwangneung forest than in the Hongneung forest. Species richness was higher in the Gwangneung forest than in the Hongneung forest, but species diversity was higher in the Hongneung forest. This dominance decreased species diversity in the Gwangneung forest. Thus, characteristics of insect community in the Hongneung forest were greatly different with that in the Gwangneung forest. This great difference of insect communities between the target and reference sites may indicate whether the Hongneung forest would not be health according to the working hypothesis, or may indicate simply the characteristic of the fragmented urban forest. In order to determine the significant decision, the repetitive studies are needed on this regard.

Lepidopteran pests monitoring in adult stage was generally performed using delta or corn typed trap including rubber septa impregnated sex pheromone (lure). Sometimes, unfortunately trapped samples were severly damaged because of biotic and/or abiotic environments such as micro-organism, predator and rain, sticky material, respectively. In our case, we monitored potato tuber moth, PTM, Phthorimaea operculella distribution during 2009~2012 in Korea. However, we encountered unexpected problem, another species can be trapped in species specific sex pheromone trap. Therefore, species confirmation was needed in trapped samples. Here we developed confirmation method by direct PCR (without DNA extraction) or sequencing methods which trapped samples that cannot identified by morphologically. We designed multi-plex PCR universal primers and species specific primers in rRNA region because to check the success of PCR and species identification. This direct PCR method can be applied in other species confirmation which monitored using pheromone trap.

The green peach aphid, Myzus persicae (Sulzer), is one of the most serious pests in cabbage cultivation. Field survey was carried out to know the insecticide resistance levels and to develop the applicable insecticide resistant markers in five main cabbage cultivation regions (Pyeong-chang, Hong-cheon, Bong-wha, Mu-ju and Je-ju) during 2009 to 2011. M. persicae can resist a wide range of insecticides in five surveyed local populations. Therefore multi resistant (MR) strain was selected from these five local populations and esterase over-expression, modified AChE (MACE) and mutation(s) in para-type sodium channel were analyzed using native IEF and quantitative sequencing with five local populations. Esterase over-expression and MACE (StoF mutation) were observed in all populations including MR strain. LtoF mutation is well known as a kdr mutation in para-type sodium channel. However, even though LC50 values of MR strain noted over 2,000 times higher than that of susceptible strain against bifenthrin, LtoF mutation was not detected in para type sodium channel and also local populations. We found another mutation (MtoL) in para and that mutation highly correlated between mutation ratio and bioassay data. For preliminary resistance monitoring, we developed quantitative sequencing (QS) to detect the frequencies of point mutation as a population genotyping. These methods can apply to manage M. persicae resistant populations in field.

Agroecosystem is a concurrence of highly complicated interaction such as crop and pest. An agroecosystem can be viewed as a subset of a conventional ecosystem, for example, to identify the pest distribution or seasonal population fluctuations etc. These are really important investigation to set the IPM strategy. With these study, now we focused on sophisticated interaction between pest and their parasite in genome and transcriptome level. We studied these interesting interaction between diamondback moth Plutella xylostella which major pests in cabbage and Diadegma fenestrale as a model endoparasitic wasp. Actually, D. fenestrale was NOT alone because it has symbiotic virus, D. fenestrale Ichnovirus (DfIV). We will deeply discussed these interaction between P. xylostella and D. fenestrale with DfIV.