The high quality of gene set is necessary to study the functional research of genes. Although perilla is cultivated as an oil crop and as a vegetable crop in Asian countries such as Korea, Japan, northeast China and Nepal, the reference genome is absent. To assembly perilla gene set, we sequenced the various tissues of perilla (Perilla citriodora) RNA-seq with Illumina HiSeq platform, generating 548,549,314 short reads. When de novo transcriptome assembly was performed with five samples, 86,396 and 38,413 transcripts were assembled as total and representative transcripts, respectively. Using 1,917,424 proteins at Phytozome ver. 9.1, we annotated the perilla assembled transcripts, and 66,139(76.55%) and 24,030(62.55%) transcripts showed the similarity with known plant proteins (E-value < 1e-10) as total and representative transcripts, respectively. Among the diverse molecular functions, we were interested in the regulatory components, such as transcription factor and transcription regulator. Using this data, we identified 499 transcripts annotated the putative transcription factor differentially expressed transcripts. 165 putative transcription factors were significantly expressed in perilla flower and 121 putative transcription factors in both leaf and flower. This study provides the perilla reference gene set and the understanding of the molecular regulation of transcription factor dependent on the tissue.
윤흑'은 내병성이 개선되고 다수성인 품종을 육성하기 위하여 다수성 계통인 유성깨에 고품질 내병성인 건흑깨를 1995년에 인공교배하여 육성한 품종으로 6년간의 계통육성을 통하여 유망계통을 선발하고 2002년부터 3년간 생산력 검정시험을 거친 후 전국 9개의 지역에서 3년간의 적응시험을 통하여 선발된 것으로 '윤흑'은 수량성이 높고 내도복성이면서 검정색 착색도가 진하고 레놀렌산 함량이 많은 고품질 품종이다. 또한 우리나라 기후조건에서 단작 및 이모작형의 광
This study is to generate SCARs markers for identification of Perilla species. A SCAR is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. We derived SCARs by sequencing and cloning the both ends of the amplified products of RAPD markers. Sixteen sequence-specific primers were synthesized from eight RAPD markers, which were completely sequenced. We developed the species-specific SCAR markers which could be used successfully in detecting genetic variation in four Perilla species. These markers could be used to verify species-origins of various forms of Perilla germplasms.
땅콩의 delta 12 fatty acid desaturase 유전자의 염기서열에서 고 올레인산을 함유하는 F435를 선발할 수 있는 PCR 기초 분자표지마커를 제작하였다. 본 연구는 포인트 뮤테이션 (point mutation)의 결과로 나타나는 고 올레인산을 SNP 마커를 이용하여 하나의 염기서열차이(아데닌 삽입)를 이용하여 판별할 수 있는 분자마커이다. 제작한 마커가 고 올레인산관련 특이 마커인지 확인하기 위하여, 일반 땅콩 9 품종과 F435를