Recently, human mesenchymal stem cells (MSCs) are attracting attention as a useful source for regenerative therapy. Controlled production of cell therapy requires the establishment and management of an accurate isolation, characterization and monitoring for quality assurance of developing MSCs mediated. In this study, we were confirmed maintenance of potency of isolated and cultured human umbilical cord (hUC)-MSCs during ex vivo expansion or after cryopreservation. Expression of their cell specific marker was analyzed by flow cytometry and the differentiation potency was confirmed by guided differentiation of adipocyte, osteocyte, chondrocyte and hepatocyte after expanding over 15 doublings in vitro. Safe production of developing a cell therapy was proved by testing for microbial, mycoplasma, endotoxin, and adventitious agents. Also stability of cells in cultivation, preservation and/or differentiation was determined chromosomal assay. In developing using hUC-MSCs, cells showed an accurate isolation and stable expansion in ex vivo condition. The results of several management assay showed that the stem cell marker expression of CD31, CD34 and CD45 were under 10%, however CD90 was over 90% by FACS analysis. Any contamination and mutation in all tests weren't detected in specific points for safe or stable production of hMC-MSCs. Also the proliferation and differentiation potency maintains during in vitro culture and after cryopreservation of hUC-MSCs. These results could be used as standard methods of maintenance of hUC-MSCs for cell therapy products and clinical application.

Near-isogenic lines (NILs) carrying bacterial blight resistance genes (Xa4, xa5 and Xa21) were developed in japonica rice using Suweon345 as genetic background. NILs were selected by gene specific DNA markers and inoculation of K1 or K3a race. NILs conferring Xa4 were resistant to K1, K2, K3, and moderately resistant to K3a. NILs conferring xa5 were resistant to K1, K2, K3, and K3a. NILs having Xa21 were susceptible to K1, while resistant to K2, K3 and K3a. Target genes of NILs with the genetic background of Suweon345 were also confirmed by using eleven Philippines races and International Rice Bacterial Blight (IRBB) NILs carrying Xa4, xa5 and Xa21. All NILs had no significant difference from their recurrent parents in the major agronomic traits except for panicle length and brown rice 1,000 grain weight. Heading date of NILs ranged from Aug. 10 to Aug. 11, which was similar to that of recurrent parent, Suweon345. Culm length, number of grains per panicle and ratio of ripened grain of NILs were similar to those of Suweon345. Milled rice of NILs was ranged from 4.82 to 4.93MT/ha. These NILs will be useful for improving resistance to K3a race of bacterial blight pathogens in Korean japonica cultivars.

A new six-rowed naked waxy barley variety, ‘Saehanchal’, was developed by the barley breeding team of the National Institute of Crop Science (NICS), R.D.A. This variety was derived from a cross between ‘SB7803G-BC6-B-B-47-2’ and ‘Suwon262’ in 1989. The fi

Boseogchal is a newjaponica rice cultivar developed from the three-way cross ofHwayoung, Tamjin and Sinseonchalat Honam Agricultural Research Institute (HARI), RDA, in 2004. It is a waxy rice with about 117 days of growth duration fromtransplanting to har

Hanmaeum is a new super-yielding japonica rice cultivar developed from a three-way cross of Mi1yang165,YR16365Acp13 and Iksan438 at Honam Agricultural Research Institute (HARI), RDA, in 2004. This cultivar has about 119 daysgrowth duration from transplant

Research Institute (HARI), NTCS, RDA, in 2004. This cultivar has a short grain shape and about 123 days growth duration from trans-planting to harvesting under the reclaimed saline area of the south-western and the mid-western coastal plain and Honam plai

Unkwang is a newjaponica rice cultivar developed from the cross between Sobibyeo (Iksan435), high yield and coldtolerance cultivar and Cheolweon 54, early maturity cultivar at Honam Agricultural Reaserch Institute (HARI), NICS, RDA, m2004. This cultivar h

Improving grain yield and quality of japonica rice continues to be a major breeding objective in Korea. In this study we evaluated genetic divergence among four japonica rice cultivars and assessed the relationship between genetic distance and hybrid perf

Sindongjinbyeo is a new japonica rice cultivar developed from a cross between Hwayeongbyeo and YR13604Acp22 line by the rice breeding team of National Honam Agricultural Experiment Station (NHAES), RDA, in 1999. This cultivar has a large grain and about 1

Manpung is a new japonica rice cultivar developed from three-way cross between Nakdongbyeo, Iri390 and Milyang111 by the rice breeding team of National Honam Agricultural Experiment Station (NHAES), RDA in 2000. This cultivar has a short grain shape and a