Lentinula edodes is an important cultivated mushroom in China. The development of Lentinula edodes production promotes more studies on it. In our previous work, degenerate PCR and chromosome walking technologies were used to obtain one pheromone receptor gene and one pheromone precursor gene from Lentinula edodes. In this study, four pairs of specific primers were designed according to the whole genome sequencing of the protoplast monokaryon of Lentinula edodes strain 135, to amplify STE3-like pheromone receptor gene and its flanking conserved genes in the protoplast monokaryon strain SUP2 derived from Lentinula edodes strain Suxiang and 33655bp DNA sequence was obtained. By BlastX search, seven putative genes were identified, and three of them are pheromone receptor encoded genes. Furthermore, near to two pheromone receptor genes, four genes encoding proteins with conserved motifs of pheromone precursors were found. This study firstly reveals the molecular organization of the B mating type locus of Lentinula edodes.

Photodynamic therapy (PDT) is a clinically approved and ra pidly developing cancer treatment regimen, It is a minimally invasive procedure that requires the administration of a photosens iti zer foll owed by the illumination of the tumor with Iigh t of an appropriate wavelength, In the presence of molecular oxygen, cytotoxic intermedi a ries a re produced‘ thus damaging cellular structures containing the photosensitizer , In the present study. we exa mined the effectiveness of newly d evelped chlorin e6- induced PDT on malignant animal tumor model of 3prague-Da wley (3D) ra t Three-week-old male 3D rats we re inocula ted s ,c, on the right f1 ank with our previously esta blished k- ras-trans formed RK3E cell line (RK3E- ras. tota l, 5xl07 cell s) , The experiments were carried out 1 week after inoculation of tumor cell s , by which time the tumors had r eached about 0,7 mm to 1.0 cm in diameter, L3-chlorin e6 (L8 Pharm Co" Gwa ngju, Korea) was admin istrated intravenous ly by the tail vein of 3D rat at a dosage of 10 mg/kg after inhalation a nesthesia of ether, Twenty- four hours a fter L8-chlorin e6 ad ministration, PDT was pe rfol‘med using a laser diode (Geumgwang Co ‘ Ltd‘’ Daejeon, Korea) a t a light dose of 100 J /cm2 and wavelength of 664 nm, A..nimals were monitered daily and tumor volume was measured by calipel The tumor t reated with PDT using Ce6 had significant reduction in tumor s ize examined by gross tumor volume , softex x- ray image, molecular imaging a nalysis, respectively, PCNA immunostaining and TUNEL assay revealed that the treat ed tu mor caused signifi cant inlübition of tumor formati on with decreased tumor cell proliferation a nd increased a poptosis , Our dat a showed Ce6-induced PDT effecti vely arrested the tumor growth by inhibi t ing cell proliferation a nd inducing a poptosis , These findings provide the potential value of Ce6- induced PDT as an a lternative candidate for a nt i- tumor therapy, Furthel bi ochemical and cellular studies will reveal the precise molecul ar mecha ni sm of cell death induced by PDT

With the evolution of industrial structure and industrialization in developing countries, some trade has become intra-industry between developing and developed countries. The level of intra-industry trade between China and South Korea has also become an essential indicator of the country’s economic development and industrialization. Since the establishment of diplomatic relations between China and South Korea in 1992, bilateral trade between China and South Korea has steadily developed, and the “Belt and Road” initiative proposed by China in 2013 and the official signing of the China-South Korea Free Trade Agreement (FTA) in 2015 have provided a good platform for the development of trade between China and South Korea. In 2017 South Korea proposed in 2017, South Korea proposed the “New North Policy”, and China and South Korea agreed to dovetail the “Belt and Road” and the “New North Policy” to achieve a superior development of China-Korea trade. However, there are still problems, such as trade imbalance and a need for a two-way flow of investment between the two sides. This paper examines the existing problems in developing China-Korea import and export trade through developing China-Korea intra-industry trade in the new era. It suggests accelerating the sustainable development of China-Korea trade from the perspectives of strengthening trade communication between the two sides and accelerating Chinese enterprises’ investment in Korea.

In line with the trend of global transport volume which has increased rapidly over the years, internal transportation in seaports is always conducted with high frequency. Thus, there is always much potential for traffic jams as well as high transportation costs and emissions. Many efforts have been initiated to streamline the inter-terminal container transport (ITT) through the development of automated vehicles and equipment as well as using private transport facilities to overcome these limitations. The purpose of this paper to develop a framework to design, analyze, and validate the efficiency of a new ITT system in a port area based on the monorail network and automatic vehicles. First, the number of shuttles and loaders was determined depending on the transport demand scenario. Next, a simulation model was applied to evaluate the system performance as well as gain more insight into the working process of the ITT system. Finally, by setting goals for the performance indicators, the results showed that the system was highly efficient with 100% of the containers delivered to their destination on time. Besides, a series of other performance tracking was provided to provide insight into the system's capabilities.

The purpose of this study was to investigate the main source of contamination of dried red pepper by assessing microbial loads on red peppers, washing water, washing machines, harvesting containers, and worker gloves that had come in contact with the dried red pepper. To estimate microbial loads, indicator bacteria (total bacteria, coliform bacteria and Escherichia coli) and pathogenic bacteria (E. coli O157:H7, Salmonella spp., Listeria monocytogenes, and Clostridium perfringens) were enumerated. The results showed that the numbers of indicator bacteria increased significantly after washing red peppers compared with that before washing (p<0.05). Moreover, E. coli and Listeria spp. were recovered from the red peppers after washing and from the ground water used in the washing process. The number of indicator bacteria on red peppers dried in the green house was lower than that on red peppers dried in a dry oven (p<0.05). However, E. coli O157:H7, Salmonella spp., L. monocytogenes, and C. perfringens were not detected. These results suggested that a disinfection technique may be needed during the washing step in order to prevent potential contamination. In addition, hygienic practices during the drying step using the dry oven, such as establishment of an optimal temperature, should be developed to enhance the safety of dried red pepper.