Guillain-Barré syndrome (GBS) is the most common cause of acute flaccid paralysis and autoimmune polyradiculoneuropathy. Campylobacter jejuni is the most commonly identified infectious trigger for GBS. A sialic-acid containing lipopolysaccharide (LPS) of Campylobacter is thought to be involved in the triggering of GBS. The galE (UDPgalactoset- epimerase) gene of Campylobacter spp. is involved in the synthesis of LPS. In this study, we detected the galE gene in Campylobacter spp. responsible for triggering the onset of GBS. The PCR assay detected the presence of the gene in 14 of the 25 (56%) Campylobacter isolates from domestic chicken, 20 of the 28 (71.4%) Campylobacter isolates from imported chicken and 50 of the 51 (98%) Campylobacter isolates from human clinical samples. Also, the specific 497-bp region of galE sequence in Campylobacters responsible for triggering the onset of GBS was amplified from GBS patient. These results could provide evidence of the first GBS-related C. jejuni infection in Korea.
본 연구는 부산물 혼합 silage 사료 급여가 한우 비육전기 거세우의 채식 및 반추행동에 미치는 영향을 규명하기 위하여 실시하였다. 시험에 사용한 공시한우는 21개월령 총 12두를 3처리로 배치하였으며, 이 때 처리구는 관행구 (C구: 농후사료+ 볏짚 자유채식)와 부산물 혼합 silage 구를 2처리 (T1: 농후사료+부산물 혼합 silage 제한급여 (1 kg) + 볏짚 자유채식, T2: 농후사료+부산물 혼합 silage 자유채식)로 하여 48시간
Total water pollution load management(TWPLM) seeks to harmonize conservation and development by allowing regional developments to be carried out in an eco-friendly manner and within the scope of achieving the desirable water quality. Water pollutant sources are managed so as to keep the total amount of pollutants in public watersheds within a certain level or total allowance in accordance with target water quality goal. With relation to the basic guideline under the Special Act on the Water Resources Management and Community Support for Watersheds, the load from the land should be determined by the classification of rainy and dried season respectively. Objective loads of abatement applied in the 1st planning period(2004∼2010) are set up standards by low flow condition(Q275) without considering the change of flow, while allowable wasteloads are estimated by the criteria of daily mean precipitation 10 mm, as critical standard for the division of surface runoff load. Even though the standard flow conditions to meet the goal of water quality at the end of unit watershed are low flow, but it might be considered to set up drought flow condition(Q355) for the improvement of water quality. Also the objective loads for abatement to meet the target water quality should be calculated without the effect of non-point sources from the land.
콜린은 정상적인 세포벽의 기능, 아세틸콜린의 합성, 지질의 운송, 그리고 매칠기의 공급원으로서 중요한 유기화합물이다. 콜린의 보충이 콜린성 신경세포의 활성을 통해 학습 및 기억력 향상에 효과가 있다는 연구 보고를 토대로, 시중에서 시판되는 많은 우유제품 속에 콜린 함유량을 증가시켜 뇌를 좋게 하는 기능성 식품으로 판매되고 있다. 따라서 본 연구에서는 효소측정법을 이용하여, 시중에서 판매되고 있는 전유와 첨가우유들을 5개 회사의 제품을 대상으로 콜린의 함량을 분석하였다. 콜린의 표준용액을 이용하여 구한 표준곡선은 0.00316의 기울기와 0.998의 r2값을 나타내었다. 회수율은 89.8-97.6 %로 양호한 값을 나타내었다. 분석한 우유에서의 콜린함유량은, 전유에서는 14.56-15.19 mg/100 g이었고, 첨가우유에서는 4.11-11.50 mg/100 g을 나타내었다.
Choline is important an organic compound for normal membrane function, acetylcholine synthesis, lipid transport, and methyl metabolism. In biological tissues and foods, there are multiple choline compounds that contribute to choline content. There are so many analytical methods for choline determination, such as radioisotopic, high-performance liquid chromatography, and gas chromatography/mass spectrometry. However, these existing methods are expensive, unmanageable, and time-consuming. In this study, we modified enzymatic method, which is applicable for the determination of choline in milk and infant formulas, and applied to bovine serum and muscle. The calibration curves were linear with higher correlation coefficients than 0.994 . Recoveries obtained by calibration curves from the spiked bovine serum and muscle samples varied between 70.6 and 85.2%. The method may be suitable for use as a routine method in the determination of choline for biological tissue and food samples.
본 실험은 NDF 및 ADF 함량이 높은 버섯폐배지(버섯잔사)에 대한 볏짚 대체 가능성을 채식 행동 관점에서 검토할 목적으로 실시하였으며, 시험사료인 농후사료+볏짚(Control: 5.32kg+자유채식), 농후사료+버섯폐배지+볏짚(T1 : 5.32kg+0.82kg+자유채식) 및 농후사료+버섯폐배지+ 볏짚 (T2 : 5.32 kg+1.64 kg+자유채식)를 급여하여 채식 행동 조사를 하였다. 공시가축은 평균체중 357.0+2.9kg인 한우 18두를 이용하
Experiment on the thermal response of college-age Korean was conducted in a thermal environmental chamber during winter season. Eight environmental conditions composed of five air temperatures (18∼26℃) and two relative humidities (40, 60%) were selected. To investigate the uniformity of thermal environments, the spatial distributions and the temporal variations of thermal parameters were examined in the test chamber. Subjects of 7 females or 7 males were questioned in the chamber for three hours, where thermal and comfort sensations were surveyed every fifteen minutes. The repeatability and temporal variation of thermal response were discussed. The thermal sensation votes of subjects were compared with the predicted mean votes calculated from the thermal parameters and human parameters. The correlation between thermal sensation votes and comfort sensation votes of subjects were discussed.
Thermal response of Korean college students was investigated in a thermal environmental chamber during summer season. Eight thermal conditions combined with four air temperatures (24, 26, 28, 30℃) and three relative humidities (40, 60, 80%) were selected. Subjects of seven females and seven males were questioned in the chamber for three hours, where thermal and comfort sensations were surveyed every fifteen minutes. During the period of thermal response experiment, four thermal parameters of air temperature, relative humidity, air velocity, and globe temperature were monitored in the test chamber. The repeatability and temporal variation of thermal response were discussed. The thermal sensation vote (TSV) of females is in good agreement with the predicted mean vote (PMV) calculated from the thermal parameters and human parameters. However, the TSV of male shows a slight discrepancy with the PMV. The neutral points of both female and male are very close to 0.18 of PMV, indicating that college-age Korean like to a slight warm environment compared with Western people. The thermal sensation vote of subjects is well correlated with the comfort sensation vote.