Azuki bean beetle (ABB), Callosobruchus chinensis (L.) is a field-to-storage pest of legumes. It is ideal to estimate its field population before it invades the stored legume stuffs. The seasonal occurrence was monitored with rocket traps supplied with synthetic female sex pheromone and with sweep netting during 2015-2016. Harvest damage was evaluated by taking purposive samples of the pods at harvest. Over-wintering stage was identified from the pod samples of left over plants. The results ascertained that October was the peak season of ABB in the azuki bean field. Grain damage and weight loss of the harvested azuki bean were 2.7% and 2.4% respectively, which could be the inoculum for storage loss. The adult emergence only from live pupae collected from pods revealed for its most probable over-wintering stage. In all, this study provides insight into some field ecological parameters of ABB that could be helpful in adopting its management strategies.

Plant essential oils (EOs) exhibit an array of biological activities against insect pests. However, their negative influences on the pheromonal activity of azuki bean beetle (ABB), Callosobruchus chinensis L., the field-to-storage pest of legumes, have not received research attentions. We evaluated the effects of three EOs and their two major components on the attractiveness of male ABBS to synthetic homofarnesal (2E- : 2Z-homofarnesal = 6:4) using Y-tube olfactometry in laboratory and rocket traps in the semi-open vinyl house. The tested EOs of Illicium verum, Croton anisatum and Gaultheria fragrantissima as well as the major components, trans-anethole and methyl salicylate ascertained significant negative effect against homofarnesal both in Y-tube olfactometry and in semi-open vinyl house tracing out a new opportunity of integrating them in ABB management programs both in field and storage.

Morphology of antennal sensilla and their distribution were investigated in male and female adults of Plautia stali,a major pest of tree fruits, using scanning electron microscopy. Antennae of P. stali was composed of a scape, a pediceland three flagella in both sexes. Four types of sensilla (s. trichodea, s. basiconica, s. chaetica, s. coeloconica) were identifiedfrom both sexes. Among them, s. trichodea and s. basiconica were multiporous, others are not. They distributed in varyingnumbers. Sexual dimorphism was not shown in the distribution of the four sensilla types. But their distribution was characterizedalong antennal segments in both sexes. This study will be useful in electrophysiological and behavioral studies on theolfactory sensory function of each morphological type of sensilla.

The spotted wing drosophila (SWD), Drosophila suzukii, is classified exotic quarantine pest in Australia and EU, soSWD-free must be supported by evidence of surveys and phytosanitary measures in exported host agricultural commoditiesin Korea. From a quarantine control point of view, SWD is importantly considered as model insect pest for exotic fruitflies (Bactrocera dorsalis) in Korea as well because of similarity in ecological cycles. In evaluations of ethyl formate(EF) only and combined cold treatment to kill eggs and larvae of SWD, the combined EF fumigation applied at LCt50%(50% killed lethal concentration X time) and cold treatement (5℃) for > 5 days showed the promise to new conceptfor eradicating quarantine pest and these could be helpful to pre-develop exotic fruit fly management in Korea.

Ethyl formate (EF) and methyl salicylate (MS) are naturally occurring and generally safe substances for use. Sub-lethaldoses of EF and MS were evaluated to find synergistic fumigation action against azuki bean beetle (ABB), Callosobruchuschinensis (L.). Susceptibility of the ABB to EF was in the order of eggs =adults >larvae >pupae. Adults was most sensitiveto MS, and followed by eggs. Larvae and pupae were highly tolerant to MS. However, in synergistic effects of EF andMS showed that all the stages of ABB were highly sensitive in the order of adults >eggs >larvae >pupae. Highest synergisticratio was found against adults when using LC10 of both EF and MS as compared to other stages. Notably, MS synergizedthe EF exposure to all stages of ABB. This finding paves the way to exploiting synergistic activity of MS and EF againstABB for the safeguard of stored azuki bean grains.

경상남도 김해시의 화포천습지 생태공원에서 2015년 4월부터 10월까지 월 1회씩 4가지의 방법을 이 용하여 육상 곤충상을 조사한 결과 총 11목 98과 281종 4,163개체가 채집․조사 되었다. 이 중 우점종은 애긴노린재(Nysius plebejus) (노린재목), 아우점종은 맵시잎벌레(Chaetocnema concinna) (딱정벌레목), 그 다음으로 알락귀뚜라미(Loxoblemmus arietulus) (메뚜기목), 그물등개미(Pristomyrmex pungens) (벌목) 순으로 조사되었다. 목별 종 수는 노린재목이 72종(25.5%)으로 가장 우점하였고, 딱정벌레목이 69종(24.6%), 파리목이 31종(13.1%)으로 그 다음으로 나타났다. 목별 개체수 또한 노린재목이 1,265개 체(30.3%)로 가장 많았고, 딱정벌레목이 911개체(21.8%), 메뚜기목이 848개체(30.4%)로 그 다음이었다. 군집 분석 결과, 우점도 지수(DI)는 0.15, 다양도 지수(H')는 4.48, 풍부도 지수(RI)는 33.60, 균등도 지수(EI)는 0.80으로 조사되었으며, 한반도 고유종 2종, 국외반출 승인 대상종 18종, 기후변화 생물지 표종 3종, 생태계 교란종 1종이 채집되었다.

주남 습지대(6.02 km 2 ) 곤충상의 다양도, 우점도, 풍부도 등을 습지대 내 3곳의 습지에서 조사하였다. 조사방법으로는 육안조사와 쓸어잡기 는 2010년, 말레이즈 트랩 조사는 2011년, 유아등 조사와 함정트랩 조사는 2012년에 실시하였다. 총 14目 141科 574種의 9,269 개체 (딱정벌레목 36.3%, 나비목 21.3%, 잠자리목 13.9%)가 조사되었다. 種數로 비교해 보면, 나비목이 31.2%로 가장 높았고, 그 다음이 딱정벌레목(28.0%) 노린 재목(12.9%) 순이었다. 우점종(총 조사개체에 대한 각 종의 개체수 비율)은 애넓적물땡땡이(Enochrus simulans, 7.9%), 꼬마줄물방개(Hydaticus grammicus, 4.3%), 일본잎벌레(Galerucella nipponensis, 4.1%), 연물명나방(Elophila interruptalis, 3.1%), 양봉꿀벌(Apis mellifera, 2.2%) 순이었 다. 세 곳의 습지에서 조사된 딱정벌레목, 나비목, 잠자리목 곤충의 수는 매우 많았지만 세 습지 간에 차이는 없었다. 주남 습지대의 곤충다양도(H’), 풍부도(RI)는 각각 5.04, 59.10이었다.

Assessments were made of fumigant and contact toxicities of 6 Myrtaceae plant essential oils (EOs) and their components against spotted wing drosophila (SWD), Drosophila suzukii (Matsumura) (Diptera: Drosophilidae). Median lethal dose (LD50;mg/L) values of Leptospermum citratum EO for fumigant activity were 2.39 and 3.24 for males and females, respectively. All tested EOs except Kunzea ambigua EO exhibited effective contact toxicity. LD50(μg/fly) values for contact toxicity of manuka and kanuka were 0.60 and 0.71, respectively, for males and 1.10 and 1.23, respectively, for females. β-Triketone fractions, composed of flavesone, isoleptospermone and leptospermone, exhibited efficient activity with the LD50 values of 0.13–0.37and0.22–0.57μg/fly for males and females, respective

The female azuki bean beetle, Callosobruchus chinensis L., a worldwide notorious pest of azuki beans produces sex pheromone having two isomer compounds; (2Z,6E)-7-ethyl-3,11-dimethyl-2,6,10-dodecatrienals (2Z-homofarnesal) and (2E,6E)-7-ethyl-3,11-dimethyl-2,6,10-dodecatrienals (2E-homofarnesal). We synthesized and evaluated the attractiveness of different blends of the two isomeric compounds using Y-olfactometry in laboratory and rocket traps in the field. The attractiveness pattern of the tested blends in Y-olfactometry was found as 1:9≥6:4>9:1 of 2E-:2Z-homofarnesal. Under field condition also, the 6:4 blend was more attractive than 9:1 blend. The result ascertained the enhanced pheromonal activity of homofarnesal with the increased ratio of Z-component in the blend. This provides an opportunity to synthesize Z-rich homofarnresal with less sophisticated and economically feasible Z-selective method.

Morphology of antennal sensilla and their distribution were investigated in male and female adults of Ooencyrtus nezarae, an egg parasitoid of Riptortus pedestris, using scanning electron microscopy. Antennae of O. nezarae was composed of scape, pedicel and seven flagella in both sexes. Six types of sensilla (s. trichodea, s. basiconica, s. chaetica, s. campaniformia, s. sickle-shaped and unknown s.) were identified from both sexes. Among them, s. trichodea and s. sickle-shaped were multiporous, others are not. They distributed in varying numbers. Sexual dimorphism was clearly observed in the distribution of s. trichodea (only on male antennae) and unknown sensilla (only on female antennae). These findings would be helpful for further studies on detailed sex specific-receptive functions of each antennal sensilla.

The spotted wing drosophila (SWD), Drosophila suzukii, is a severe pest of berry fruits like strawberry, blueberry, sweet cherry and grape in terms of quarantine. For the disinfestation of SWD, methyl bromide (MeBr) is the only option in quarantine practice. However, MeBr's current use will be phased out due to chronic inhalation toxicity to fumigators and related worker as well as ozone depletion properties. To replace MeBr, we evaluated the efficacy of ethyl formate (EF) and phosphine gas (PH3) to all different developmental stages of SWD at practical fumigation condition (>25 ℃). To achieve complete control of all stages of SWD, PH3 required > 6 hr exposure when applied at > 1.0 g m-3. In case of 4hr EF fumigation, the LCt99 (LCt99, 99% lethal concentration × time) of EF to egg, larvae and pupae of SWD was 198.09, 77.42 and 115.55 g·h m-3, respectively.

Euzophera batangensis (Lepidotera: Pyralidae) is seriously damaging trunks or branches of persimmon tree (Diospyros sp.). We tested the attractiveness of (Z)-9-tetradecen-1-ol (Z9-14OH) and (Z9,E12)-tetradeca- 9,12-dien-1-ol (Z9,E12-14OH) with single or blended baits at southern parts of Korea in 2014 and 2015. The monoene was not attractive at all at three places during the two years. In 2014, diene was equally or more attractive than the mixture in Jinju and Suncheon, respectively. In 2015 too, the attractiveness of diene and mixture was not different in Jinju and Munsan. Monitoring of the seasonal occurrence of E. batangesis with the sex pheromone components revealed that it occurred three times a year; the first occurrence from early April to mid May, the second from early Jun to mid July, and the third from late August to late September.

Ooencyrtus nezarae lshii (Hymenoptera: Encyrtidae) is a major egg parasitoid of Riptortus pedestris (F.) (Hemiptera: Alydidae). One of the components of the aggregation pheromone of R. pedestris, (E)-2-hexenyl (Z)-3-hexenoate (E2HZ3H), is known to attract O. nezarae female. Response of O. nezarae females to E2HZ3H and its isomers was studied using electro-antennogram (EAG) and field tests. O. nezarae females showed higher antennal response to E2HZ3H and Z2HE3H isomers than E2HE3H and Z2HZ3H. In field tests, O. nezarae females were attracted to E2HZ3H traps, but not to Z2E3 traps. Interestingly, the blend of E2HZ3H and Z2HE3H showed significant antagonistic activities to the attraction of O. nezarae females in the field. We conclude from this study that Z2HE3H is a potent antagonist to the behavioral response of O. nezarae.

The bulb mite (BM), Rhizoglyphus echinopus, is hard to control in the harvested onion and garlic. Methyl bromide (MeBr) fumigation is the only option to eradicate BM at present. However, MeBr causes phytotoxic damage (index 3) to onion and garlic and highly toxic to fumigators. To replace MeBr, we evaluated ethyl formate (EF) and phosphine gas (PH3) to BM at 5℃ which is current practical fumigation temperature. The >140 g·h m-3 of EF for 24 h could completely control BM and this CT (concentration × time) product was slightly (index 1) phytotoxic to onion and garlic. Fumigation of 2 g PH3 required >120 h exposure to kill 100% of BM without any pyhtotoxic damage. Further researches are being planned to shorten the exposure time with synergistic effect between EF and PH3.

애기유리나방과 복숭아유리나방의 성페로몬 트랩을 이용하여 2013년부터 2015년까지 경남과 전남지역의 단감원에서 이들의 발생소장을 조사하였다. 두 종 모두 년 2회 발생하는 것으로 조사되었다. 애기유리나방의 1화기의 발생최성기는 5월 하순, 2화기는 9월 중·하순인 것으로 나 타났다. 복숭아유리나방의 1화기 발생최성기는 해에 따라 조금씩 달라 5월 상순 - 6월 상순 정도이었고, 2화기 발생최성기는 애기유리나방처럼 9 월 중·하순으로 조사되었다. 년도별 지역별 유인량을 종합해보면 애기유리나방은 1세대의 유인량이 2세대보다 약 1.9배 많았으나 복숭아유리나 방의 경우에는 이와 반대로 2세대의 유인량이 1세대보다 약 1.3배 많았다. 그러나 복숭아유리나방의 감나무에 대한 피해여부는 아직까지 밝혀진 바가 없다.

Twelve Lamiaceae plant essential oils (EOs) and their components were evaluated for their fumigant and contact toxicities. Inhibition of AChE by the major components of active EOs was also assessed. Strong fumigant toxicity was observed from EOs of Mentha piperita and Perilla frutescens. Menthone and menthol, and perilla aldehyde were identified as major components of the above two EOs, respectively. The LC50 (mg/L) values of M. piperita, P. frutescens, menthone, menthol and perilla aldehyde were 3.87, 2.44, 5.76, 1.88, and 0.99 against male, and 4.10, 3.31, 5.13, 1.94, and 1.15 against female, respectively. Strong contact toxicity was observed from the EOs of Satureja montana and Thymus zygis. Thymol and carvacrol were major components of these two EOs. The LD50 (μg/fly) values for contact toxicity of S. montana, T. zygis, thymol and carvacrol were 2.95, 2.93, 1.63, and 1.30 for male, and 4.59, 5.09, 2.68, and 2.60 for female, respectively. Among the five major components, perilla aldehyde showed most active inhibition activity against AChE of both sexes of SWD.

Six wooden plant essential oils (EOs; Illicium verum, Gaultheria fragrantissima, Bursea delpechiana,Croton anisatum, Cinnamomum cassia and Aniba rosaeodora) and their major compounds (trans-anethole, methyl salicylate, trans-cinnamaldehyde, linalool and linalyl acetate) identified from gas chromatography (GC) and GC-mass spectrometry were tested for adult repellence and pheromone antagonism using Y-tube essay and oviposition deterrent effect using no choice test against adzuki bean beetle (ABB), Callosobruchus chinensis L. EOs of I. verum and C. anisatum as well as their common major compound, trans-anethole were found effective repellents having high degree of pheromone antagonistic and oviposition deterrent activity. Methyl salicylate, the major compound of G. fragrantissima EO (which was only the pheromone antagonist) showed high degree of repellency, oviposition deterrence and pheromone antagonistic effect in higher concentration. From this study, EOs of I. verum and C. anisatum and their common major compound trans-anethole as well as the major compound of G. fragrantissima, methyl salicylate can be screened as eco-friendly management agents against C. chinensis in stored legumes if slow releasing formulations are prepared by future efforts.

Drosophila suzukii is an economically important pest of fruit in America and Europe as well as in Asia. Sparassol (1) and methyl orsellinate (2) are produced by Sparassis cripta and S. latifolia during culture. Fumigant and contact toxicities of synthetic sparassol (1) methyl orsellinate (2) and methyl 2,4-dimethoxy-6-methylbenzoate (DMB; 3) were investigated. The possible mode of action of the compounds was pursued by assessing their inhibition activities against acetylcholinesterase (AChE) and glutathione S-transferase (GST). Little or no fumigant activity was observed from tested compounds. In contact toxicity activity, LD50 (μg/fly) values are 5.29, 1.18, 0.02 for male, and 11.14, 2.27, 0.05 for female, respectively. In enzyme inhibition assay, DMB (3) which easily synthesized from sparassol could be an alternative agent for controlling insect pest. In addition, Sparassis species could be an industrial resource of sparassol.

Four sympatric tortricid (Lepidoptera) species in New Zealand, Epiphyas postvittana (Walker), Planotortrix octo (Dugdale), Cydia pomonella (L.), and Cydia succedana (Denis and Schiffermüller), were studied for the morphology of antennal sensilla and the chemical communication systems using scanning electron microscopy (SEM), electro-antennogram (EAG), gas chromatography-electroantenno-detection (GC-EAD), single sensillum recording (SSR) and field tests. Antennal flagella of adult have four main types of olfactory sensilla (s.); s. trichodea, s. basiconica, s. auricillica, and s. coeloconica in the four species. The s. trichodea subtype I was longest and male-specific in all the species. The antennae of males of the four species showed characteristic higher EAG, GC-EAD and SSR responses to 12, 14-carbon acetates or alcohol. Some of the selected chemicals selected from the electrophysiological experiments caused significant antagonistic or agonistic activities to the sex pheromone of each species in the field. In this study, the four species showed distinct chemical communication systems according to sub-family, which possibly serve as a factor in species isolation.

Insect antennae play important roles in finding mates and in locating food source and oviposition sites. Riptortus pedestris is an important pest of soybean and sweet persimmon in Korea. The male R. pedestris adult produce the aggregation pheromone attracting the conspecific nymphs and both sexes of adults. The pheromone was known as a cue for food finding, but the 1st instar nymph can develop to the 2nd instar without food. This phenomenon may suggest that the 1st instar nymph may have different sensilla system from other instars. Thus, we investigated the morphology and distribution of antennal sensilla and antennal response to the aggregation pheromone (AG) of each nymphal stage using Scanning Electron Microscope (SEM) and Electroantennography (EAG). As expected, first instar nymph did not have sensilla trichodea 3 (T3) and chaetica 3 (Ch3) which existed in other instar nymphs. The antennae of the 1st instar nymph did not responded to AG, with no difference from control. For further elucidation of the functions of sensilla T3 and CH3, single sensillum recording to AG will be done.