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        검색결과 109

        41.
        2018.10 구독 인증기관·개인회원 무료
        꽃노랑총채벌레는 저항성 해충군이 가지고 있는 크기가 작고 산란수가 많으며 세대기간이 매우 짧은 특성을 가지고 있다. 또한, 이동능력이 우수하고, 다양한 령기가 혼재하고 있으며, 꽃과 잎 등 식물체뿐만 아니라 토양에서 생활환을 완성하는 복잡한 생태를 가지고 있다. 이러한 총채벌레의 특성으로 인하여 시설 내 방제는 어려운 실정이며, 동일계통의 작용기작을 갖는 약제들의 단조로운 패턴의 약제사용으로 인하여 그 방제효과는 떨어지고 있다. 본 연구에서는 꽃노랑총채벌레의 효과적인 방제를 위한 약제 혼용살포, 연속살포, 로테이션 처리별 효과 검증을 통하여 방제체계를 제안하였다. 우선 실내에서 약제별 약충과 성충에 대한 처리방법별 효과 검토를 통하여 꽃노랑총채벌레 밀도가 높지 않은 초기에 사용이 가능한 약제와 총채벌레 밀도가 높은 시기에 처리할 수 있는 약제를 선발하였고, 이를 기반으로 포장에서 혼용살포, 연속살포, 로테이션 조합별 효과 검토를 하였다. 또한 본 시험결과들을 기초로 ㈜경농에서는 3가지 이상의 다른 작용기작의 약제를 밀도가 높을 경우 3~5일 간격으로 3회 이상 연속살포하는 3!3!3! 총채벌레 관리프로그램을 운영중이며 지속적으로 관리프로그램을 개선해 나가는 중이다.
        42.
        2018.10 구독 인증기관·개인회원 무료
        딱정벌레목 바구미과에 속하는 나무좀은 일찍부터 산림의 주요 해충으로 다루어져 왔다. 최근 사과원에서 M.9 대목을 이용한 사과 밀식재배가 확산되고, 기후변화에 따른 이상 기상으로 사과나무 동해에 의한 2차 나무좀 피해가 늘어나고 있어 이들에 대한 관리대책이 필요한 실정이다. 본 연구는 사과원에 발생하는 나무좀류의 발생 양상을 파악하고 효과적인 약제 선발을 통해 효율적인 방제 체계 개발을 위한 기초 자료로 활용하고자 수행하였다. 사과원에 발생하는 나무좀은 암브로시아나무좀, 오리나무좀, 붉은목나무좀, 사과둥근나무좀 등 4종으로 암브로시아나무좀이 우점종으로 조사되었다. 암브로시아나무좀의 발생은 4월부터 시작하여 연 2회 발생하였다. 주간부에서 나무좀의 피해는 대부분 21~100cm 높이에서 발생하였으며, 야외 방제 시험에서 Phentoate EC, Chlorpyrifos WP, Thiacloprid SC 등이 높은 방제가를 나타내었다.
        44.
        2017.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study aimed to identify milling characteristics depending on the number of a cutting roller’s air vent and blowing velocity to remove rice bran by the cutting type milling machine which can minimize the conventional milling process. The level of whiteness was found to be 38±0.5 in all the conditions, showing consistent whiteness levels during milling. The rice temperatures turned out to be 15.4 and 14.6oC which were rather low-level under the conditions of the cutting roller with 3 vents and blowing velocities of 35 and 40 m/s respectively. Cracked rice ratio was 2.13% under the conditions of the cutting roller with 3 vents and a blowing velocity of 35 m/s. Broken rice ratio showed the range of 0.762-0.869%, reflecting a low level. Turbidity after milling was decreased, as blowing velocity became faster. Energy consumption for milled rice production was decreased, as blowing velocity became faster. The optimum milling condition for cutting type milling machine depending on air vent number of cutting roller and blowing velocity was found to be 3 vents and 35 m/s.
        4,000원
        45.
        2017.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study aimed to identify milling characteristics depending on the number of a cutting roller’s air vent and blowing velocity to remove rice bran by the cutting type milling machine which can minimize the conventional milling process. The level of whiteness was found to be 38±0.5 in all the conditions, showing consistent whiteness levels during milling. The rice temperatures turned out to be 15.4 and 14.6oC which were rather low-level under the conditions of the cutting roller with 3 vents and blowing velocities of 35 and 40 m/s respectively. Cracked rice ratio was 2.13% under the conditions of the cutting roller with 3 vents and a blowing velocity of 35 m/s. Broken rice ratio showed the range of 0.762-0.869%, reflecting a low level. Turbidity after milling was decreased, as blowing velocity became faster. Energy consumption for milled rice production was decreased, as blowing velocity became faster. The optimum milling condition for cutting type milling machine depending on air vent number of cutting roller and blowing velocity was found to be 3 vents and 35 m/s.
        4,000원
        46.
        2017.04 구독 인증기관·개인회원 무료
        The technique of artificial insemination is the possible one that can surely control mating of the selected drones with the virgin queen. This technique was first time applied with A. cerana in Korean. The average amount of semen can collected from one Korean A. cerana drone was 0.09 μl and 0.1 μl of Vietnamese one, whereas the A. mellifera was more than 6 times (0.58 μl semen per A mellifera drone). Obtaining 1 μL of semen have to collect from 11.94 drones that successful semen ejection. Queens artificially inseminated with 4 μl of semen (once insemination) or 8 μl of semen twice (each insemination with 4 μl of semen) started laying egg later than naturally mated queens 5.3 and 2.5 days, respectively. The onsets of oviposition of artificially inseminated queens were 12.5 to 15.3 days.
        47.
        2017.04 구독 인증기관·개인회원 무료
        The Small hive beetle, Aethina tumida (Murray, A. 1867) (commonly abbreviated to SHB), is one of notifiable pest in the world. SHB were confirmed in the southeastern United States in 1998 in a apiary in Florida. The SHB also was found in Korea in 2016. The SHB larvae have relatively large heads and numerous protuberances covering their bodies. Upon full maturation, larvae have reached a length of 10 mm. Adult females are length and width of ± 5.5 mm and ± 3.4 mm. Naturally occurring small hive beetles can vary greatly in size, possibly depending on diet, climate, and other environmental factors. Additionally, we were analyzed the mitochondrial cytochrome oxidase I (COI) gene of the SHBs. The sequence of the COI gene of SHB was identical to that of SHBs in Korea, but different by fourteen positions from Italy.
        48.
        2017.04 구독 인증기관·개인회원 무료
        Due to their environmental and economical consequences, invasive species have become a major concern worldwide. Among them, the yellow-legged hornet, Vespa velutina, is a keen hunter of domestic honeybees. They use olfactory cues from the prey to assess food information. In this study, we investigated the attraction behavior of V. velutina to honeybee pheromone under outdoor conditions. Nine honeybee pheromones were tested and compared in order to find the best attractant. When testing specific compounds, the honeybee queen pheromone, homovanillyl alcohol, proved highly attractive.
        49.
        2017.04 구독 인증기관·개인회원 무료
        The diamondback moth, Plutella xylostella, is known worldwide as a pest that affects cruciferous plants. We surveyed insecticide susceptibility in chlorantraniliprole-resistant strain of P. xylostella to 13 insecticides (3 diamaids, 2 synthetic pyrethroids, 2 spinosyns, 1 organophosphorus, 1 carbamate, 1 marcrolide, and 3 others) to examine resistance ratios. The levels of insecticide resistance were extremely high, especially to chlorantraniliprole and flubendiamide. Enzyme assays indicated that glutathione S-transferase (GST) activity in the resistant strain was 2.4 times higher compared with the susceptible strain, whereas no difference was seen for P450 and esterase. Its F1 progeny resulting from reciprocal crosses with a susceptible strain revealed no maternal effects and a diamide susceptible phenotype, suggesting an autosomally almost recessive mode of inheritance.
        50.
        2016.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Earthworms, especially Eisenia andrei, are cultivated for a variety of purposes including waste disposal and compost production. In this study, bacteria from earthworm gut were cultured and the dominant species identified. Subsequently, we isolated bacteriophages able to lyse the isolated gut bacteria. Two dominant genera of gut bacteria, Aeromonas sp. and Citrobacter sp. were identified by using MALDI-TOF MS analysis and a library was constructed to find lytic phages. Phage EF1 showed lytic activity for C. freundii and two Citrobacter isolates, C. braakii and C. murliniae. These 3 species have similar sensitivities to EF1. Several aspects of the life cycle of EF1 were investigated by using C. freundii under optimal growth conditions. EF1 infects C. freundii with a moderate latent period, approximately 25 min, and a large burst size averaging 5 × 109 per infected cell. Moreover, the antimicrobial activity of EF1 was well maintained under diverse conditions including a broad temperature range of 40°C to 50°C and a wide pH range of 4 to 11. In conclusion, the results indicate that earthworm casting contain a wide range of bacteria species, for which there are various corresponding bacteriophages.
        4,000원
        52.
        2016.10 구독 인증기관·개인회원 무료
        Zika virus, mosquito-borne disease, caused by mosquitoes has been increased the importance. From March to September, twice a month from 7 different points (3 residential areas, 3 migratory bird sanctuary and 1 cattle shed) were collected using BG trap and BL trap. After identifying the mosquitoes collected, we confirmed the virus infection. Total 26,531 mosquitoes (6 genus 9 species) were collected, virus has been detected from the 3 species (Aedes vexans, Cuilex tritaeniorhynchus, and Amigeres subalbatus) of mosquitoes of them. It showed the highest peak in August, and then gradually decreased. The most common mosquito species was collected Aedes vexan (16,637) in the cattle shed.
        53.
        2016.10 구독 인증기관·개인회원 무료
        The Asian tiger mosquito, Aedes albopictus, is considered as potential vector of Zika virus in Republic of Korea (ROK). Vector control during mosquito season is one of critical factors for decline of viral transmission. Total 14 oversea travel-associated Zika cases by mosquito bite were reported throughout ROK from January to September 2016 and vector control and monitoring at surroundings of patient’s residences was carried out during three weeks after confirmation of the virus. Although population density rates of Ae. albopictus were remarkably various according to ecological surroundings, population density of Ae. albopictus near forest was higher than urban. All captured Aedes mosquitoes were used for detection of three flavivirus, Zika, Dengue and Chikunguya, using RT-PCR and any virus was not detected. Population density of Ae. albopictus decreased > 65% on average after vector control and in one area > 95% of population density decreased. Our data might reveal that vector monitoring and control at surroundings near residences of oversea travel-associated Zika patients might effectively prevent viral transmission by mosquito bite and naturalization of the virus in ROK.
        55.
        2014.04 구독 인증기관·개인회원 무료
        Screening for antimicrobial peptide genes in the immune-induced Antheraea yamamai larvae led to the identification of a novel antifungal moricin-like peptide (MLP10) gene. The complete MLP10 cDNA is comprised of 403 bp with 174 bp open reading frame encoding a 58 amino acid precursor that contains a putative 23-residue signal peptide, a 2-residue propeptide and a 33-residue mature peptide. The deduced amino acid sequence of MLP10 has 26∼52% identity to those of moricin-related peptides from lepidopteran insects. The MLP10 was highly expressed in E. coli BL21(DE3) by fusing with ketosteroid isomerase (KSI) to avoid the cell death during induction. The resulting expressed KSI-MLP10 fusion protein was in a insoluble form. Recombinant MLP10 was released by cleavage of the fusion protein with cyanogen bromide (CNBr). Subsequently, we purified pure active MLP10 by FPLC chromatography, and 5.2mg of MLP10 was obtained from 1L culture medium. The purified MLP10 was prevented the growth of candida albicans at 6.25 uM, and was also active against gram negative and gram positive bacteria. This potent antimicrobial activity suggests that MLP10 may play a role in the immune response of A. yamamai.
        56.
        2014.03 구독 인증기관 무료, 개인회원 유료
        Pseudomembranous colitis (PMC) is known to be associated with the long-term administration of antibiotics, which alter normal gastrointestinal flora and allow overgrowth of Clostridium difficile. However, antituberculosis agents are rarely reported as a cause of this disease. Besides, most cases of antituberculosis agent-induced PMC have been observed in patients with pulmonary tuberculosis but not with tuberculous meningitis. This report presents a case of PMC associated with antituberculosis therapy in a patient with tuberculous meningitis. A 29-year-old female patient was admitted due to headaches and diplopia that had lasted for 2 weeks. She had not recently received antimicrobial therapy. She was diagnosed with tuberculous meningitis by cerebrospinal fluid findings and neurologic examination, including brain imaging study. She was treated with standard antituberculosis agents (HERZ regimen: isoniazid, ethambutol, rifampicin, and pyrazinamide). After 11 days of HERZ, she developed a fever, sudden widespread skin eruption, and elevation of liver enzymes. Considering adverse drug reactions, antituberculosis agents were stopped. One week later, her symptoms were relieved. Thus, antituberculosis agents were reintroduced one at a time after liver function returned to normal. However, she presented with frequent mucoid, jelly-like diarrhea, and lower abdominal pain. Sigmoidscopy revealed multiple yellowish plaques with edematous mucosa, which were compatible with PMC. She was treated with oral vancomycin considering drug interactions. Symptoms were relieved and did not recur when all antituberculosis agents except pyrazinamide were started again. Therefore, when a patient complains of abdominal pain or diarrhea after initiation of antituberculosis therapy, the physician should consider the possibility of antituberculosis agent-associated PMC.
        4,000원
        57.
        2013.12 구독 인증기관 무료, 개인회원 유료
        Muscle satellite cell (SC) is responsible for postnatal muscle growth, repair, and regeneration. Satellite cell is an im-portant source of multi-potent stem cell process and differentiation into adipogenic, myogenic, and osteoblastogenic. The objective of this study was to identify alter of transcriptome during differentiation in porcine satellite cell and to elevated transcriptome at different stages of postnatal development to gain insight into the differences in differ-entiated PSC. We used RNA-seq technique to investigate the transcriptomes during differentiation in pig muscle. Sequence reads were obtained from Illumina HiSeq2000. Differentially expressed genes (DEG) were detected by EdgeR. Gene ontology (GO) terms are powerful tool for unification among representation genes or products. In study of GO biological terms, functional annotation clustering involved in cell cycle, apoptosis, extracellular matrix, phosphoryla- tion, proteolysis, and cell signaling in differences stage. Taken together, these results would be contributed to a better understanding of muscle biology and processes underlying differentiation. Our results suggest that the source of DEGs could be better understanding of the mechanism of muscle differentiation and transdifferentiation.
        4,500원
        58.
        2013.12 구독 인증기관 무료, 개인회원 유료
        Satellite cells were derived from muscular tissue in postnatal pig. Satellite cell is an important to growth and development in animal tissues or organs. However, the progress underlying induced differentiation is not clear. The aim of this study was to evaluate the morphologic and the transcriptome changes in porcine satellite cell (PSC) treated with insulin, rosiglitazone, or dexamethasone respectively. PSC was obtained from postnatal muscle tissue. In study 1, for study the effect of insulin and FBS on the differentiated satellite cells, cells were cultured at absence or presence of insulin treated with FBS. Total RNA was extracted for determining the expression levels of myo-genic PAX3, PAX7, Myf5, MyoD, and myogenin genes by real-time PCR. Myogenic genes decreased expression levels of mRNA in treated with insulin. In study 2, in order to clarify the relationship between rosiglitazone and lipid in differentiated satellite cells, we further examined the effect of FBS on lipid accumulation in the presence or absence of the rosiglitazone and lipid. Significant differences were observed between rosiglitazone and lipid by FBS. The mRNA of FABP4 and PPARγ increased in rosiglitazone treatment. In study 3, we examined the effect of dexame-thasone on osteogenic differentiation in PSC. The mRNA was increased osteoblasotgenic ALP and ON genes treated with dexamethasone in 2% FBS. Dexamethasone induces osteoblastogenesis in differentiated PSC. Taken together, in differentiated PSCs, FABP4 and PPARγ increased to rosiglitazone. Whereas, no differences to FBS and lipid. These results were not comparable with previous reports. Our results suggest that adipogenic, myogenic, and osteoblasto-genic could be isolated from porcine skeletal muscle, and identify culture conditions which optimize proliferation and differentiation formation of PSC.
        4,000원
        59.
        2013.12 구독 인증기관 무료, 개인회원 유료
        Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90∼100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson’s, oil red O, and Alizarin red staining respectively. We per-formed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator- acti-vated receptor gamma (PPARγ) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteo-blast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were indu-ced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strat-egies for augmenting meat quality.
        4,000원
        60.
        2013.10 구독 인증기관·개인회원 무료
        Antimicrobial peptides (AMPs) can be produced in mealworms, currently being used as animal feeds, by the infection of genetically engineered-entomopathogenic fungi. In this work, we integrated Bombyx mori (Bm) AMP, cecropin A to Beauveria bassiana ERL1170 by restriction enzyme-mediated integration method, which was confirmed by RT-PCR and an antibacterial activity assay. For the extracellular secretion of Bm cecropin A protein, the active domain of the cecropin A gene was tailed to the signal sequence of B. bassiana chitinase (Bbs). To exchange Bbs-cecropin A gene with egfp gene in pBARKS1-egfp, Bbs-cecropin A fragment was cut from pGEM-Bbs-cecropin A using XbaI/blunted and BamHI and ligated with cut pBARKS1-egfp using NcoI/blunted and BamHI, designated as pBARKS1-Bbscecropin A. After the transformation, transformants were grown on Czapek’s solution agar containing 600 μg ml-1PPT. Expression of Bm cecropin A was confirmed by RT-PCR. Strong clear zone was observed in the co-culture of the transformant D-6 and Bacillus subtilis on fourth strength Sabouraud dextrose agar 1 day after the culture at 25°C, whereas the wild type had no clear zone. This work suggests that Bm cecropin A can be efficiently produced in this mealworm-based fungal expression platform, thereby increasing the value of mealworms in the animal feed additive industry.
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