Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase (SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability, compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.

The objective of this study was to investigate the response of seed germination based on seed treatment of supercritical carbon dioxide (SC-CO2) sterilization. Seeds of eleven species were treated with SC-CO2 at 150 bar, 55℃ and 15 min (condition of fungi sterilization). Standard germination tests were used to evaluate germinability of seeds. Compared with non-treated seeds, SC-CO2 treatment caused significant variation of germination rate in different species. Different species showed different response to SC-CO2 treatment. The results suggest that crop of Leguminosae family is suitable for SC-CO2 sterilization.

A new kidney bean cultivar, “Noghyeob 1” was developed at the Yeongnam Agricultural Research Institute (YARI) in 2005. “Noghyeob 1” was selected from a cross between KLG50074 and KLG50063. It has determinate growth habit, white flower, green pod color, oval shape of crossed section of pod at the harvesting time for edible pod, white seed coat and middle seed size (21.1 grams per 100 seeds). The average yield of edible pod of “Noghyeob 1” was 24.25 M/T per hectare in the yield trials which were carried out at the green house in spring and autumn in 2005. It was 7 percent higher than that of the check cultivar “Kangnangkong 1”.

A new six-rowed naked waxy barley variety, ‘Saehanchal’, was developed by the barley breeding team of the National Institute of Crop Science (NICS), R.D.A. This variety was derived from a cross between ‘SB7803G-BC6-B-B-47-2’ and ‘Suwon262’ in 1989. The fi