Red mite, Dermanyssus gallinae is one of the serious pests, damaging to the egg production in chickens. Moreover, the overuse of chemical insecticides caused pest resistance and environmental residual toxicity. Therefore, this work provides a screening method to select entomopathogenic fungi as a candidate of environmentally safe control agent, having high miticidal activity against D. Gallinae. The virulence test was conducted using a spraying method. Of the several species, Beauveria species showed high virulence and mycosis were observed. Some isolates were produced on eight cereal grains in Petri dish conditions. Millet, perilla seed and barley showed the high conidia production. Conidia of the isolates produced on millet and rice showed high thermal stability, when exposed to 45℃. Based on these results, this work suggests that entomopathogenic fungi could be used to control the D. Gallinae.

본 연구는 Deci와 Ryan의 자기결정성이론을 바탕으로 게임사용 동기가 게임행동에 미치는 영향에 대해서 분석하였다. 게임사용 동기는 내재적 동기와 외재적 동기로 구분하였으며, 게임행동은 게임 선용과 게임과몰입으로 구분하였다. 분석을 위해 한국콘텐츠진흥원의 2016년 게임 과몰입 실태 조사의 자료 중 3,000명의 성인을 대상으로 실시한 온라인 조사의 자료를 활용하였다. 자료는 잠재변 수간의 관계를 알아볼 수 있는 구조방정식모형 분석방법을 통해 분석하였다. 분석은 Mplus 7.4 프 로그램을 활용하여 실시하였다. 본 연구의 결과는 다음과 같다. 첫째, 내재적 동기는 게임선용과 정적인 관계가 있었다. 둘째, 외재적 동기는 게임선용과 게임과몰입과 정적인 관계가 있었다. 게임과 몰입과의 관계의 효과크기가 게임선용과의 관계의 효과의 크기보다 세 배 이상 크게 나타났다. 본 연구는 한국사회에 만연한 게임에 대한 부정적인 인식에서 벗어나, 게임으로 인한 긍정적인 결과와 부정적인 결과에 대하여 균형 있는 접근을 시도하였다는 점에서 의의가 있다. 또한 자기결정성이론을 바탕으로 게임사용자와 게임제공자의 긍정적인 게임사용을 촉진하기 위한 시사점을 제시하였다.

Insect-killing fungi have high potential in pest management. A deeper insight into the fungal genes at the whole genome level is necessary to understand the inter-species or intra-species genetic diversity of fungal genes, and to select excellent isolates. In this work, we conducted a whole genome sequencing of Beauveria bassiana (Bb) JEF-007 and characterized pathogenesis-related features and compared with other isolates including Bb ARSEF2860. A large number of Bb JEF-007 genes showed high identity with Bb ARSEF2860, but some genes showed moderate or low identity. The two Bb isolates showed a significant difference in vegetative growth, antibiotic-susceptibility, and virulence against Tenebrio molitor larvae. When highly identical genes between the two Bb isolates were subjected to real-time PCR, their transcription levels were different, particularly in heat shock protein 30 (hsp30) gene which is related to conidial thermotolerance. In several B. bassiana isolates, chitinases and trypsin-like protease genes involved in pathogenesis were highly conserved, but other genes showed noticeable sequence variation within the same species. Given the transcriptional and genetic diversity in B. bassiana, a selection of virulent isolates with industrial advantages is a pre-requisite, and this genetic approach could support the development of excellent biopesticides with intellectual property protection.

갈색날개매미충은 기주 범위가 넓고, 효과적 방제가 어려운 해충으로 알려져 있으며, 향후 더 큰 피해를 유발할 수 있는 해충으로 지목되고 있다. 경북지역의 안동과 의성에서 3월부터 9월까지 4개 지점을 선정하여 갈색날개매미충의 월동란이 산란된 월동기주를 중심으로 월동란 부화 시점 및 부화 후 약충에서 성충까지 그리고 다시 월동란 산란까지의 패턴을 모니터링하였다. 각 지점의 환경조건 및 수목류 군락의 조성은 조금씩 차이가 있으나 충분한 산란흔이 있었다. 1령 약충은 5월 중하순부터 하순까지 나타났고 산수유나무, 매실나무에서 가장 먼저 확인되었으며, 이후 수목류에서의 밀도증가 양상은 매실나무에서 더 빠르게 진행되었다. 그리고 2령 약충 까지는 기주식물에 인접한 수목류로의 이동한 모습도 일부 관찰되었다. 그러나 6월 중순경에는 3령 약충의 기주식물 주변 초본류로의 이동이 관찰되었으며, 월동란에서의 부화 후 1회차 이동분산으로써, 주로 상부에서 하부로의 점프를 통한 이동으로 판단된다. 이는 새순 부위가 경화된 월동 기주식물에서 경화되지 않은 초본류의 새순으로의 이동으로써, 특정한 초본류에 집중되지 않고 넓은 범위에 산재하여 분포하였다. 4령 약충 시기에는 주변 특정 초본류와 목본류 새순에 집중적으로 분포하는 패턴을 보이기도 하였다. 7월경에는 성충이 관찰되었으며, 이는 2회차 분산이 진행되는 시점으로 판단된다. 이후 8월 중순까지 지속해서 밀도 감소가 이뤄지던 월동 기주에서의 밀도 감소 상태가 증가 추세로 전환되었다. 그리고 9월 상순경에는 2018년 월동기주에서의 성충밀도 증가와 교미 행동 및 1년생 가지에서의 산란흔이 관찰되었다. 산란흔의 집중도는 산수유나무와 매실나무가 다른 수목류보다 높았으며, 이는 모니터링이 진행된 모든 지점에서 공통된 패턴임을 확인할 수 있었다.

Longhorned tick, Haemaphysalis longicornis (Ixodida: Ixodidae) is one of the vectors of severe fever with thrombocytopenia syndrome virus (SFTSV) in human. The tick occurs in mostly grass fields, and the use of pyrethroid insecticides induced pest resistance and environmental residual toxicity. Particularly the use of chemicals near residential areas where persons live become a big issue, so environmentally safe control agents need to be considered. Here in this work, our interest was given to the selection of highly virulent fungi against H. longicornis. A total of 101 fungal pathogens collected from mountainous areas were assayed by a dipping the nymph stage of ticks into a conidial suspension (1×107conidia/ml). Interestingly among several species, one species showed high virulence and mycosis were observed in 7-15 days. Most of the selected isolates produced a large amount of conidia in Italian millet, rice and millet with thermotolerance at 40-45°C. Based on these results, we selected a couple of isolates with high virulence against H. longicornis and they could be used for the control of the ticks after the set-up of a practical application strategy in fields by optimizing fungal colonization in soil and phyllosphere.

Thrips is one of the serious insect pests in many economic crops, in which general managements rely mainly on chemical insecticides. However, the overuse of chemicals led to the development of insect resistance and environmental residual issues. Therefore, thrips management requires the consideration of alternative strategies which are less harmful to the environment and working on different target points. In this work, we isolated entomopathogenic fungi from soil, and identified them with morphological and molecular biology methods, followed by a preliminary virulence assay against Tenebrio molitor larvae. Selected fungal isolates were adjusted to 1×107 conidia/ml for an indoor virulence assay against thrips adults, and highly virulent isolates were added to a thrip-pathogenic fungal library. Biological characteristics of efficacious isolates were investigated comparing to a previously commercialized fungal isolate. This entomopathogenic fungal library could be used as a valuable resource for developing effective strains to control thrips in agricultural fields.

Entomopathogenic fungi have been known as promising candidates for biological control of insect pests. Recently, researchers consider the fungal thermotolerance in formulations and field applications. In this study, we investigated the production of thermotolerant Isaria javanica and I.fumosorosea conidia through grain-based solid cultures and exposure to light stress. As results, of the ten grain substrates, Italian millet, rice, perilla seed and sesame, rice, sorghum produced highly thermotolerant conidia in the strains. The two strains were exposed to a light stress and showed enhanced thermal stability compared to control, when exposed to 45°C for 2 hours. This work suggests that heatresistant entomopathogenic fungal conidia can be produced by grainbased solid cultures and exposure to light stress.

Whitefly has a broad host range of more than 600 species, and 40 biotypes have been reported worldwide. Among the various ecological types, B and Q are the most problematic. Especially, Biotype Q, which causes serious damage in the green house, mediates more than 40 kinds of viruses, including Tomato yellow leaf cull virus (TYLCV). The development of resistance to whitefly, adverse effects on non-target organisms, and strengthened legal regulations are making chemical control difficult in application. For these reasons, the use of alternative strategies is needed to suppress the Biotype Q whitefly population. Entomopathogenic fungi were investigated and developed to control hard-control insect pest, such as whitefly. These fungi are natural pathogen of their invertebrate host and contribute to the regulation of their host population in the environment. In addition, these agents are typically harmless to humans and non-targeted organisms and do not adversely impact the environment. Therefore, in this study, we screened pathogenic fungi against whitefly using our entomopathogenic fungal library, and virulence tests were conducted with conoidal suspension (1×107conidia/ml) in laboratory conditions. As a result, 10 isolates showed 80-100% mortality at 5 days after treatment. We will analyze the characteristics of these fungal isolates and evaluate their potential as insecticides against whitefly. The selected isolates against the high mortality of whiteflies can be effectively used in integrated pest management programs for whiteflies.

Japanese pine sawyer beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae) is considered as a serious pest in pine trees. To develop an eco-friendly strategy to manage this forest insect, we collected entomopathogenic fungi from Korean soil and assessed their virulence against the adults of the insect in laboratory conditions. As a result, two isolates with conidial suspension (1.0×107conidia/ml), showed 87% and 90% mortality 12 days after fungal treatment, respectively. We assessed the potential of the fungi-derived destruxin and protease as additives to the fungal isolates, and they showed insecticidal activity via feeding and spraying treatments. Finally, we produced fungal conidia in massive solid cultures and formulated wettable powders, and now studying optimal conditions of oil-based formulation with two isolates. Based on these results, we are evaluating the control efficacy of the fungal agents against M. alternatus in field conditions.

최근 봄철 기온이 높아짐에 따라 마늘 뿌리응애 피해가 늘어나고 있는 실정이다. 이러한 뿌리응애 피해를 최소하 하고자 발생양상과 22종의 약제에 대하여 살비효과를 조사하였다. 마늘 재배시기 중 뿌리응애의 발생은 충북 보은 등 5지역에서 2015년부터 2017년까지 3년간 3월부터 6월까지 뿌리응애 발생밀도를 조사하여 발생양상을 확인하였다. 뿌리응애는 2016년에 가장 많이 발생하였으며, 발생시기는 월동 후 3월 상순부터 발생하여 6월 중순경까지 지속적으로 밀도가 높아지는 양상을 나타내었다. 조사된 5지역 중 가장 높은 밀도로 조사된 보은지역은 6월 상순경 주당 13.3마리가 발생하였다. 뿌리응애에 대하여 우수한 살비효과를 나타낸 약제는 분무처리시 약제처리 10일 후에서 dimethoate, azocyclotin, abamectin, hexythiazox, cabosulfan, spirotetramat, benfuracarb가 90% 이상의 살비효과를 나타내었다

Japanese pine sawyer, Monochamus alternatus Hope (Coleoptera: Cerambycidae) in forest is considered as a serious forest pest in pine trees. In this study, we collected entomopathogenic fungi from soil and assessed their virulence against the adults of the inset using a spray method in laboratory condition. Two entomopathogeinc fungi isolates showed high virulence against the adults. In the concentration of 1.0 × 107conidia/ml, the mortality rate of the adults were 53% and 60%. In semi-field condition, one isolate showed a virulence of 60% against the adult. Consequently, we confirmed the possibility of the fungal isolates in controlling the beetles. In near the future, we will investigate several factors which is possibly related to the control of the forest insect pest using entomopathogenic fungi in field conditions, given the importance of fungal formulation and practical application methods.

The hard tick, Haemaphysalis longicornis (Ixodida: Ixodidae) is one of the vectors of severe fever with thrombocytopenia syndrome virus (SFTSV) causing in humans, however little consideration was given to the biological control of the pest. Therefore, this study provides a screening method to select entomopathogenic fungi, having high virulence against H. longicornis. The virulence test was conducted by dipping method with a conidial suspension (1×107 conidia/ml). As a result, cadavers of H. longicornis infected with entomopathogenic fungi were obtained by this method. Based on this results, we selected several isolates having high virulence to H. longicornis. We suggest that, selected isolates in this study can be used for the control of H. longicornis.

Entomopathogenic fungi have been known as promising candidates for biological control of insect pests. Recently, researchers consider the fungal thermotolerance in formulations and field applications. In this study, we investigated the production of thermotolerant Isaria javanica and I.fumosorosea conidia through grain-based solid cultures and exposure to light stress. As results, of the ten grain substrates, Italian millet, rice, perilla seed and sesame, rice, sorghum produced highly thermotolerant conidia in the strains. The two strains were exposed to a light stress and a heat stress. And they showed enhanced thermal stability compared to control, when exposed to 45°C for 2 hours. This work suggests that heat-resistant entomopathogenic fungal conidia can be produced by grain-based solid cultures and exposure to light stress.

Melon thrips, Thrips palmi Karny (Thysanoptera: Thripidae) is a serious insect pest of various crops in the world. The management of this pest mainly have relied on chemical agents. However, the overuse of chemical agents for long times causes insect resistance, and negative effects on environment. Therefore, alternative control methods, such as biological control, that are less harmful to the environment and have different mode of action are needed to control this pest. In this study, we isolated entomopathogenic fungi from Korean soil, and characterized them via morphological and molecular identifications and pathogenicity check against Tenebrio molitor larvae. The isolated fungi were subjected to virulence assay against T. Palmi with a conidial suspension of 1×107 conidia/ml in laboratory conditions. This entomopathogenic fungi library would be a good resource to select effective strains for the control of T. palmi in fields.

Entomopathogenic fungi play an important natural role in regulating their insect host populations, and their ecology was also associated with plant and soil. These microorganisms have been living by reacting to insect, plant and environmental factor. The advanced bioinformatics technology such as next-generation sequencing and RNA sequencing has revolutionized in understanding of entomopathogenic fungi. Recently studies provided a lot of information on evolutionary relationships and virulence-related characteristics. We are starting to know where these microorganisms from is, and how they live in nature. The bioinformatics technology will give us further our understanding of the natural roles of these fungi in nature.

태양농장은 1982년 누에고치 농사로 시작된 대한민국 대표 곤충산업 선도 기업 중 하나로서 경상북도 영덕에 소재하고 있다. 태양농장에 대한 개략적인 소개, 온라인 마케팅 현황, 농장 내 사육공정의 소개 및 부가가치 창출방안을 소개 하고자 한다. 동충하초 재배 과정의 초기 먹이 수급 및 누에사육 과정, 접종, 건조, 동충하초 재배 및 수확과정을 제시한다. 우리 농업의 전망과 성공 노하우, 수요창출기법 양잠 산업의 전망 등을 제시한다.