Emotional sharing of brand has a power of connecting brand and consumer brand and consumer by transcending rational reason.

Social media play a role in the value that sneakerheads, individuals who collect, wear, and trade sneakers with extraordinary effort and resources, place on specific brands and models of sneakers. In-depth interviews with 25 sneakerheads, recruited through sneakerhead online communities and snow-ball sampling, were conducted to explore how social media contribute to emotional and monetary value creation of sneakers, as well as how social media is used across the Inventory Ownership Cycle (IOC) which includes pre-acquisition, acquisition, physical possession, and disposal of sneakers (Boyd & Mcconocha, 1996). Finding shows that during the pre-acquisition stage, emotional and monetary value is constructed when brands post images of celebrities wearing new models on various social media sites. Hype initially created by the brand is perpetuated by posts and conversations among sneakersheads. Perceived higher levels of hype lead one to believe demand surpasses supply, and therefore, monetary values of specific models increase. Through social media, consumers research and gauge the value not only of soon to be released sneakers, but also previously released sneakers only available on the resale market. During the acquisition stage, the online environment has become increasingly important. To avoid issues associated with selling high-demand/low-supply sneakers in- store, such as violent incidents and dishonest employees, many retailers have shifted sales of such product to online formats (Dunne, 2014). Social media serves as a platform for sneakerheads to learn about dates and locations of new releases and to locate resellers from whom they can purchase previously released sneakers. During the physical possession stage, sneakerheads engage with community members via social media; they show off their sneaker collections, as well as to obtain and share information regarding sneaker customization and maintenance to preserve aesthetics and value. It is through the engagement with fellow sneakerheads and the sneakers within their personal collections that emotional value can be further constructed. During the disposal stage, social media is used by resellers to determine monetary value, identify and communicate with potential buyers across the nation, and complete resale transactions. In summary, this research demonstrates how sneakerheads use social media throughout the IOC, as well as the role social media plays in the construction and identification of sneaker value.

Maturation-promoting factor (MPF) is well-known as cell cycle regulator during oocyte maturation and fertilization. MPF activity maintains high levels and arrest the cell cycle progression until fertilization. After fertilization, Anaphase-promoting complex/cyclosome (APC/C) mediated degradation of cyclin B causes decrease of MPF activity. One of the cytostatic factor (CSF), Emi2 inhibits APC/C activity by binding to APC/C-cdc20, therefore blocks the proteolysis of cyclin B. Degradation of Emi2 requires phosphorylation by Polo-like kinase 1 (Plk1). Thus recognition and phosphorylation of Emi2 by Plk1 are essential step for meiotic cell cycle resumption. In our previous research, we found that two phosphorylated threonine regions at amino acid position 152 and 176 in Emi2 are respectively contributed for recognition by polo-box domain of Plk1. Peptidomimetics 103-8 can block the interaction between Plk1-PBD and Emi2, and therefore meiotic maturation and meiosis resumption via parthenogenetic activation were impaired. However, major drawback of 103-8 was the limitation of penetration through the cell membrane. We synthesized the new peptidomimetics and checked bioavailability in mammalian oocyte by injection and media treatment. Medium treatment with peptidomimetics C-4, meiotic maturation has significantly decreased and meiotic resumption via parthenogenetic activation has perfectly impaired. For the next experiment, we are preparing X-ray crystallography to identify the binding modes between Plk1-PBD and peptidomimetics C-4.

Substantial knowledge about how electronic word of mouth (eWOM) relates to influentials, or, individuals who either intentionally or unintentionally have a great impact on the network, is lacking because influence patterns are hardly quantifiable when human and societal components are not deeply understood. The eWOM networks in social media platforms drive the theoretical and practical progress of the social structure of eWOM and the social role of influentials. The purpose of this study is to explore the diffusion of eWOM in a Twitter network by drawing from the Diffusion of Innovation theory in conjunction with Social Network Analysis (SNA) approach. Through a large amount of data collected from Twitter regarding the terrorist attack in Paris on November 13, 2015, this study examined the social structure of eWOM network groups and identifies the social role of influential users in the network of #prayforparis. To identify influentials of eWOM in Twitter networks, SNA was conducted with NodeXL, which is an Excel add-in network analysis software. The Twitter data set was uploaded at 11 am, on November 17, 2015 by applying hashtag prayforparis through Twitter Search Network feature of NodeXL. A total of 19,592 tweets (vertices) were downloaded with a total of 20,297 edges (relations between vertices). “Justin Bieber” was identified as the most influential and dominant user in the network of #prayforparis because he has the highest in-degree (4,196) and highest betweenness centrality (51,772,745.861). Justin Bieber lost one of his friends in the terrorist attack, and his tweet about the sadness from the loss was retweeted more than 50,000 times and received 77,000 likes. Throughout the network, top word pairs respectively repeated more than 4,000 times were “thomas, prayforparis,” “rt, justinebieber,” “rip, thomas,” and “justinbiber, rip.” “Ally Brook” and “Louis Tomlin” ranked 3rd and 5th respectively with the highest betweenness centrality also demonstrated the celebrity power in the eWOM network of #prayforparis. While the three celebrities as dominant influentials were prominent in the Twitter network of #prayforparis, the social roles of fans were also acknowledged. The celebrities’ fans and fan sites have constantly diffused the celebrities’ posts by retweeting and by mentioning every tweet posted by the celebrities that they support. Without the celebrities and their fans, the #prayforparis network would have depicted a completely different style of diffusion of eWOM. The study confirmed social role of fans that constantly presented idiosyncratic manners by identifying and by linking to the public figure. The distinctive characteristics of the fans also enabled identifying the social role of the celebrity while spreading the new ideas with hashtag. The celebrity played a key role as an information hub even in a network of socio-political events such as the #prayforparis Twitter network. Thus, identified overwhelming influence of the celebrities in the eWOM network extends the areas of marketing choices and applicable strategies with celebrities.

Well-dispersed Ag3VO4 nanoparticles @polyacrylonitrile (PAN) nanofibers were synthesized by an easily controlled, template-free method as a photo-catalyst for the degradation of methylene blue. Their structural, optical, and photocatalytic properties have been studied by X-ray diffraction, transmission electron microscopy, field-emission scanning electron microscopy equipped with rapid energy dispersive analysis of X-ray, photoluminescence, and ultraviolet–visible spectroscopy. The characterization procedures revealed that the obtained material is PAN nanofibers decorated by Ag3VO4 nanoparticles. Photocatalytic degradation of methylene blue investigated in an aqueous solution under irradiation showed 99% degradation of the dye within 75 min. Finally, the antibacterial performance of Ag3VO4 nanoparticles @PAN composite nanofibers was experimentally verified by the destruction of Escherichia coli. These results suggest that the developed inexpensive and functional nanomaterials can serve as a non-precious catalyst for environmental applications.

The bacterial lipopolysaccharide (LPS) mainly contributes to the structural integrity, survival and protection barrier against harsh environments. Therefore, the early stages in LPS or lipid A biosynthesis are attractive targets in the identification and development of inhibitors which would be effective against infections caused by Gram-negative bacteria. The bacterial outer membrane proteins (OMPs) meanwhile function as maintenance for structure, adhesion to other cells and substances, as well as development of resistance to antimicrobials. The LPS and LPS-related molecules, and OMPs are important immunogenic components of several important pathogens including Brucella, which have been extensively used in immunological studies and in the diagnosis of diseases. Here we review the importance, structure, functions and immunogenic aspects of LPS and OMPs particularly of Brucella which can be targeted for the prevention and diagnosis of brucellosis.

Hydroxyapatite (HA), which is an important calcium phosphate mineral, has been applied in orthopedics, dentistry, and many other fields depending upon its morphology. HA can be synthesized with different morphologies through controlling the synthesis method and several parameters. Here, we synthesize various morphologies of HA using two simple methods: hydrothermal combustion and solution combustion. The phase purity of the synthesized HA is confirmed using X-ray diffractometry. It demonstrates that pure phased hydroxyapatite can be synthesized using both methods. The morphology of the synthesized powder is examined using scanning electron microscopy. The effects of pH and temperature on the final powder are also investigated. At 140 oC, using the hydrothermal method, nano-micro HA rods with a hexagonal crystal structure can be synthesized, whereas using solution combustion method at 600 oC, a dense cubic morphology can be synthesized, which exhibits monoclinic crystal structures.

To date, most serodiagnostic methods for brucellosis screening are based on antibodies against lipopolysaccharides of Brucella spp. However, this approach has the drawback of yielding false-positive results due to cross-reactivity with lipopolysaccharides of other related pathogens, especially Yersinia enterocolitica O:9. In this study, Brucella abortus AspC was cloned and expressed by PCR amplification into a pCold TF expression system to obtain recombinant AspC (rAspC). The immunogenicity of rAspC was confirmed by western blotting of Brucella-positive bovine serum. rAspC-based ELISA was performed to determine whether rAspC could be used in the serodiagnosis of bovine brucellosis. rAspC reacted strongly with anti-Brucella antibodies in positive sera in the tube agglutination test (TAT), but did not show strong reaction with most negative samples. In particular, the average OD492 value at the highest TAT titer showed a 1.4-fold increase with respect to the cutoff value. The accuracy, specificity, and sensitivity of rAspC were 71.88%, 78.33%, and 68%, respectively. These findings suggest that rAspC might be valuable for the serological diagnosis of bovine brucellosis.

Brucellosis is an important and re-emerging zoonotic disease worldwide. The prevention of human infection is achieved predominantly through the control of brucellosis in agricultural animals, which in turn depends on accurate diagnosis and vaccination. However, conventional serological diagnosis of brucellosis has several limitations, and currently available vaccines for animals have several drawbacks, including the ability to cause infection in humans. Phosphoglycerate kinase (Pgk) is one of the specific proteins reactive with mouse sera in the early stage of Brucella infection, and deletion of the pgk gene in B. abortus strain 2308 resulted in extreme attenuation of this strain in vitro and in vivo. Furthermore, the B. abortus pgk mutant has been used as a live vaccine, and in challenge experiments, it induced protection that was superior to that conferred by commercial strains. In this study, the pgk gene from Brucella abortus 544 was successfully amplified and cloned into a maltose binding protein fusion protein expression vector (pMAL). The recombinant protein was expressed in Escherichia coli DH5α and purified. The immunogenicity of purified recombinant B. abortus 544 Pgk (rPgk) was evaluated by western blot analysis using Brucella-positive mouse sera. rPgk could be used as an antigenic component for future serological tests and potential vaccine development.

This study examines the effect of SPA store experience on formation of customer equity. Here we distingush dimension of store experience to sensory, affective, behavioral, intellectual, and relational experience and dimension of customer equity to value, brand, relationship equity. To verify relationship between dimension of store experience and customer equity, as well as loyalty, we use structural equation modeling. As a result, relationship between variables have a significant effect on each other exclude hypotheses such as relationship between affective experience and value equity and relationship between behavioural experience and relationship equity. The result indicates that reinforcing SPA store experience likely to have positive impact on formation of customer equity and loyalty strength.

This study examines the effect of SPA store experience on formation of customer equity. Here we distingush dimension of store experience to sensory, affective, behavioral, intellectual, and relational experience and dimension of customer equity to value, brand, relationship equity. To verify relationship between dimension of store experience and customer equity, as well as loyalty, we use structural equation modeling. As a result, relationship between variables have a significant effect on each other exclude hypotheses such as relationship between affective experience and value equity and relationship between behavioural experience and relationship equity. The result indicates that reinforcing SPA store experience likely to have positive impact on formation of customer equity and loyalty strength.

This study explores the moderating role of perceived sponsor motive and event-self congruence on the relationship between event-sponsor congruence and attitude toward sponsor in CSR advertisingcampaigns. A scenario based 2 (event-sponsor congruence: high/low) ⅹ 2 (perceived sponsor motive: selfish/altruistic) ⅹ 2 (event-self congruence: high/low) between-subjects design experiments are conducted for the test of hypotheses. According to the results of ANCOVA, consumers' perceived sponsor motive and event-self congruence are shown to moderate the effect of event-sponsor congruence on consumers' attitude toward sponsor. And, when the consumer perceives sponsor motive as ‘altruistic’ and event-self congruence as ‘high’, the difference of attitude toward sponsor between the high group and low group of event-sponsor congruence perception is the lowest.