Bacillus thuringiensis kurstaki와 aizawai의 내독소단백질을 알카리용액 또는 트립신, 곤충소화액 드응ㄹ 처리하여 전기영동한 후 단백질팬턴을 비교하였다. 두 균주의 주요 결정단백질은 130kd와 64kd의 단백질이었으며, 소화액이나 효소로 처리한 경우 공통적으로 62kd의 활성독소가 생성되었다. 그러나, aizawai는 kurstaki에 비해 현저히 적은 양의 62kd 단백질을 생성하였다. 흰불나방의 유충이 Bacillus thuringiensis 독소를 섭식하였을 때 지방체를 비롯한 몇 가지 조직에서 45kd의 스트레스 단백질이 유발되었는데 이 단백질은 열충격이나 저온 충격시에도 마찬가지로 생성되었다.

Purpose – The current study is to compare the cognition of stakeholders on hosting a mega sports event between Korea and the United States. In particular, to understand their cognition and perceptual conflict towards hosting a mega sports event, the study employed conflict theory. Furthermore, the study reviewed the role of social capital in the process of managing the mega sports events. Research Design, Data, and Methodology – Of homogeneous sampling, purposeful sampling method and criterion-based selection approach were used to collect interview data from key stakeholders who have been involved in hosting a mega sports events in Korea and the United States. In-depth interview transcripts were reviewed multiple tiems after transcription to extract concepts and meanings that were pertinenet to the experience involving hosting a mega sports event. Further member checks was conducted to increase the credibility of the results. Results – Results can be summarized as followed: First, stakeholders of Korea have a strong desire for positive economic effects of a mega sports event, compared to those in the United States who are more concerned in enhancing the public interests and concerns. Second, in Korea, various socio-political issues emerged at the same time and conflicts among multiple stakeholders have aggravated the situations to coordinate the issues. This was because legal system supporting socio-trust has not been established. On the other hand, major stakeholders of the United States consisted of community members who have socio-trust and networks. Thereby these social resources have been found playing a key role in building social capital that assists the stakeholders to coordinate the current issues and to solve them. Conclusions – The current study analyzed the cognition and perceptual conflict of stakehoders in a mega sports event. Social capital has beend found as a key catalyst to increase a network and cooperation among stakeholders. In order to enhance social capital in managing a mega sports event hosted in Korea, legal systems that establish networks and relationships among the related stakeholders need to be developed. Furthermore, the systematic guideline needs to be developed, organizing the sub-committees according to the types of stakeholders and the categorized common needs.

To evaluate the protective effect of Ajuga multiflora BUNGE (AMB) extract on the toxicity of lead acetate (LA), environmental pollutant, cell viability was measured by XTT assay using cultured NIH3T3 fibroblasts. And also, the effect of antioxidant, butylated hydroxytoluene (BHT) on LA-induced cytotoxicity was analysed. For the protective effect of AMB extract on LA-induced cytotoxicity, NIH3T3 fibroblasts were pretreated with 80 or 90 μg/mL of AMB extract for 2 hours before the treatment of LA. And also, the antioxidative effects of AMB extract against LA-induced cytotoxicity were assessed by DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation (LP). In this study, LA significantly decreased cell viability dose-dependently compared with control, and then XTT50 value was determined at 46.1 μM of LA. In the effect of BHT against LA-induced cytotoxicity, it effectively prevented toxic effect of LA by the significant increase of cell viability. In the protective effect of AMB extract on LA-induced cytotoxicity, it significantly increased cell viability which was decreased by LA-induced cytotoxicity, and also it showed the antioxidative effects such as DPPH-radical scavenging activity, SOD-like activity and inhibitory activity of LP. From these results, it is suggested that the cytotoxicity of LA is involved in oxidative stress, and AMB extract effectively prevented the cytotoxicity induced by LA via an antioxidative effect. Conclusively, the natural substance such as AMB extract may be alternative resources for the prevention or treatment of diseases related with oxidative stress.

Background : Recently, hair loss, which has been regarded as a mere means of middle-aged men due to stress and environmental pollution. The market for hair loss in Korea is about four trillion won and it is growing continuously. It is mainly made by mixing natural extracts such as medicinal plant. The purpose of this study was to investigate the effects of ethanol extracts of Houttuynia cordata Thunb. whole plant and Calendula officinalis L. flower extracts on the growth of fibroblasts, dermal papilla cells and lipid precursors, I want to try to make a materialization. Methods and Results : The cytotoxicity of each sample extracts treated with 50%, 100%, and 500 μg to fibroblasts, cell-viability were 107.3%, 109.6%, and 128.2%, respectively. The cytotoxicity of each sample to the dermal papilla cells was not observed. And the lipid differentiation of the lipogenic precursor cells which regulates the hairegeneration by secretion of the platelet derived growth factor. The 70% ethanol extracts of H. cordata whole plant and C. officinalis flower were showed promotes the hair growth activity. The lipolysis rate was significantly increased with increasing treatment concentration Conclusion : As a result of this study, in-vitro hair growth activity of herbal medicines for hair treatment material development was not shown to be toxic to each cell. And 70% ethanol extract of H. cordata whole plant stimulated lipid precursor cells inducing differentiation. As a result, the 70% ethanol extracts of H. cordata whole plant and C. officinalis flower have potential to developing hair-related product.

Anthocyanin, a group of purple or reddish flavonoids, have been recognized as health-promoting functional food ingredients due to antioxidant activity. For this reason, plant breeders are trying to increase the anthocyanin contents using methods such as classical breeding and biotechnological approaches. To broaden the mutants population, seeds of colored wheat variety (K4191) were irradiated by using 250 Gy gamma irradiation. Individual 968 M4 plants were grown in Korea Atomic Energy Research Institute field. Many mutant phenotypes were shown: seed color variation, abnormal spike shape, awning formation, heading and ripening time, plant height, ripening period, super dwarf, etc. To identify the inheritance traits of colored-wheat, individual lines were maintained the spike base classified by generation. Characteristics per spike and plant were piled up to construct for mutant database. In the future, fixed descent will be analyzed the anthocyanin contents or other phytonutrients by ultra-performance liquid chromatography (UPLC). Expression of seed color-related transcription factors and anthocyanin biosynthetic pathway genes will be examined.

Over the last decades, increasing natural disasters and climate change are considered as the major environmental problems facing the globe. Numerous studies have been indicated it would cause huge losses on agriculture, especially in the grain productivity. Therefore, several alternatives are suggested for boosting up productivity of wheat as one of the main human food crop. One of important strategy is proper management of inflorescence development and DELLA proteins have been elucidated to play pivotal roles in growth of many plant organs. In this study, putative negative regulator of DELLA protein, GAST (Gibberellic acids stimulated transcript) have been isolated to identify their role in the developing spike of wheat. Four genes were isolated from its gene family and designated as TaGAST1, 2, 3, 4. Genomic structure was analyzed to demonstrate chromosomal localization of TaGAST genes and evolutionary relationships were also verified with GAST genes in other plant species. RT-PCR was conducted to detect transcriptional changes of TaGAST genes on external phytohormone. Each of TaGAST genes showed considerable changes in transcription level after GA, ABA, PAC treatment, respectively. Through Yeast two-hybrid assay, one protein for TaGAST1, and four proteins for TaGAST2 was isolated as putative interactive proteins in wheat spikes just before and after emergence.

Rye has important genes for biotic and abiotic stress resistance. Introduction of these genes to wheat by breeding wheat-rye translocation have been intensively used in wheat breeding program. Rye chromatin 1RS and/or 2RL show superior performance in unfavorable environments. In order to develop high yielding wheat, we applied various molecular breeding strategies. To develop EST-derived 1RS specific markers, we used comparative genomics with public sequence databases of Poaceae family. Putative rye chromatin specific sequences were used to design 1RS specific markers. To identify genes related to water deficiency, cDNA AFLP analysis was used in PEG treated seedlings of 1RS RILs. For functional analysis of identified genes and markers, we used Brachypodium distachyon, as a new model plant of temperate grasses. B. distachyon were recently applied for transformation and we constructed Agrobacterium-mediated transformation system. Integration of those strategies and conventional breeding method would enhance the usefulness of rye chromatins for wheat improvement.

barley grain and malt is highly related to beer quality, especially hordein is known to be a more significant factor in malting process than albumin. In this study, we proposed selection criteria for high quality malting barley with aid of grain and malt quality parameter scores and storage protein subunit profile informations. Albumin and hordein were extracted and denatured protein subunits were evaluated with malt and grain quality parameters. Total 13 local adaptability test (LAT) lines were planted in four locations (Naju, Iksan, Jeju, and Jinju) and evaluated for malt and beer making qualities. Seventeen germplasms (world collections for high or low seed storage protein content) were also evaluated for biochemical genetic marker. Denatured seed storage protein subunits of albumin and hordein of all tested lines and germplasms were evaluated using 12% 1D SDS-PAGE. Scored data of protein subunit's presence or absence was applied to Agglomerative Hierarchical Clustering (AHC) for statistical analysis. Subunits fractionated within specific molecular weight ranges (97.4-31.0, 66.2-31.0, and 45.0-31.0 kDa) were highly correlated with agricultural characteristics. Several LAT lines showing good performance in agricultural characteristics were clustered in dendrogram constructed by biochemical-genetic assay using XLSTAT. Specific band pattern showed in good performance LAT lines were also observed in some germplasms of world collection having low protein contents which are known to have superior quality in malting. The results would provide selection criteria for high quality malting barley in the malting barley breeding program.

The direct use of mutation is a valuable approach to generate variability in crops. The electron beam, one of the ionizing radiations, has been applied to evaluate its effect on seed germination and early seedling growth of creeping bentgrass (Agrostis palustris Huds., cv Penn-A1). The mature dry seeds were irradiated with various electron beam energies (0.3, 1.0, 1.3, and 2 MeV) and current levels (0.03 and 0.06 mA). Although large variability was existed within each dose, distinct difference of germiability and seedling vigor were not found at 0.3 MeV / 0.03 mA and 0.3 MeV / 0.06 mA beam condition. However, 1.0 MeV / 0.06 mA application most effectively inhibited and retarded seed germination and most severely restricted cotyledon and root growth in early seedling growth. The direct use of electron beam would be a valuable supplementary approach to generate mutants suitable for breeding purposes.

Universal AIS, which has been adopted officially for automatic identification systems among regulated ships by SOLAS, should be installed, for example, on all passenger ships over 300 tons engaged in international voyage and over 500 tons in domestic voyage, sequentially from 2002 to 2004. We must not overlook the fact than-ruled regions by regional authorities in the case of VTS. Actually a major portion of accidents have happened in small vessels like fishing vessels. However, they are not equipped with automatic identification tools, due to the high costs of the equipment for identifying purposes, as well as the absence of regulation In this paper, we researched the alternative of automatic identification for small vessel instead of universal AIS. We analyzed the requirement of automatic identification for small vessel about wireless communication method, traffic volume, etc. We proposed the identification system for small vessels in local areas and developed the Local Vessel Identification System (LVIS) interoperable with universal AIS using a PDA platform and wireless network