The aim of this study was to provide a basis for the molecular mechanism underlying the pharmacological action of ethanol. We studied the effects of 1-propanol on the location of n-(9-anthroyloxy)palmitic acid or stearic acid (n-AS) within the phospholipids of synaptosomal plasma membrane vesicles (SPMV). The SPMV were isolated from the bovine cerebral cortex and liposomes of total lipids (SPMVTL) and phospholipids (SPMVPL). 1-Propanol increased the rotational mobility of inner hydrocarbons, while decreasing the mobility of membrane interface, in native and model membranes. The degree of rotational mobility varied with the number of carbon atoms at positions 16, 12, 9, 6 and 2 in the aliphatic chain of phospholipids in the neuronal and model membranes. The sensitivity of increasing or decreasing rotational mobility of hydrocarbon interior or surface by 1-propanol varied with the neuronal and model membranes in the following order: SPMV, SPMVPL and SPMVTL.

This study is investigated the growth characteristics(number of available stipe, pileus diameter, pileus thickness, stipe length, stipe thickness, object weight, comparison yield ability of 1 cycle) and storage characteristics of ‘Sanjo 701’ (S7) cultivars according to relative humidity. The S7 growth characteristics were investigated by quantifying the growth and the characteristics according to the relative humidity, The storage stability was investigated every 5 days and freshness was measured by ‘Minamide Method’. S7 pileus diameter is The higher the relative humidity was confirmed becomes larger and the more increased relative humidity also increase the comparison yield ability of 1 cycle. However, pileus diameter or stipe length This could not see the big difference in the three treatment groups, the plieus thickness was no significant difference in the treatment of 80% and 95%. The fresh weight of S7 decreased significantly at 80% and 95% relative humidity after 10 days of storage, but decreased continuity in 65% humiditiry. The elongation percentage of S7 pileus was observe in 95% relative humidity, values of L, a and b (SCI), showed the highest L value in 65% and the a value in 95%. b values were similar in 65%, 80% and 95% treatments. On the ‘Minamide Method’ measure freshness was changed from the 10th day of storage at relative humidity of 95%, but humidity of 65% and 80% treatments, it changed after 15 days of storage. In this study growth characteristics and yield were increased at higher relative humidity, but storage stability was decreased. Therefore, it is deemed necessary to change the relative humidity to produce high quality mushrooms.

목 적 : 우세안과 비우세안의 결정 요인들을 조사하고, 성인 남녀의 성별에 따른 차이를 비교분석하였다. 방 법 : 성인 92명을 대상으로 Hole-in-the-card method로 우세안을 결정하였고, 안압, 등가구면굴절 력, 난시도, 카파각을 측정하여 우세안과 비우세안의 차이를 성별로 비교하였다. 결 과 : 성별에 따른 안압(p=0.570), 등가구면굴절력(p=0.465)은 통계적으로 유의하지는 않았다. 우세안 에서 남자의 난시도가 유의하게 크게 나타났으며, 양안 난시도에서도 남자의 난시도가 유의하게 크게 나타 났다(p=0.012). 카파각은 우세안과 비우세안에서 여자의 카파각이 유의하게 나타났으며, 양안 카파각도 여 자의 카파각이 통계적으로 유의하게 나타났다(p=0.002). 결 론 : 우세안과 비우세안의 요인을 성인 남녀의 성별로 비교하였을 때, 남자의 난시도와 여자의 카파각 이 유의하게 크게 나타났다.

‘Baekseung’, a new variety of Flammulina velutipes, was bred by mating two monokaryotic strains isolated from KMCC 4210 and KMCC 4216 in Mushroom Research Division, Baekseung ARES in 2016. Baekseung showed fast mycelial growth and high mycelial density on MEA (Malt Extract Agar) media for 7days of incubation. Spawn running period on the sawdust substrate required 30days at 25°C. The cultivation period and optimum temperature were 11±1 days at 14°C for primordia formation and 14±1 days at 7°C for fruiting body development. The length of pilei and stipes in Baekseung harvested in optimal stage exhibited 11.3±0.4㎜ and 89.2±7.1㎜ and Megumi harvested in optimal stage showed 8.2±1.0㎜ and 95.9±5.0㎜ respectively. Yield of Baekseung and Megumi strain grown of sawdust substrate was 153.7±12.5g and 150.5±29.7g per 850ml in bottle cultivation. The inferred tree exhibited the difference of phylogenetic relationship between the Korean white fruiting body strains such as Baekseung, Uri1ho, Fv-14-a-38, and Fv-14-a-51 and the Japanese white fruiting body strain Megumi.

목 적: 핀홀의 크기를 변경하면서 피검자의 소수시력을 측정하였으며, 핀홀의 크기가 시력, 등가구면 굴 절이상, 난시도에 주는 영향을 연구하였다. 방 법: 성인 118명, 평균나이 22.7±5.9세를 대상으로 핀홀의 크기를 0.5 ~ 3.0 mm로 하고, 굴절이상에 따른 소수시력을 측정하였다. 동공크기에 따른 시력을 계산하여, 측정된 소수시력과 비교하였다. 결 과: 핀홀의 크기가 감소하면, 소수시력이 고도근시(-6.96±0.83 D)와 중등도근시(-4.30±0.83 D)에 서는 크게 증가했으며, 약도근시(-1.63±0.64 D)는 핀홀크기 1.0 mm에서 정점을 나타냈다. 정시 (-0.11±0.37 D)와 원시(1.88±1.63 D)는 약간 감소하였다. 동공의 크기가 감소하면 종구면수차와 종색수차 가 서서히 감소하고, 피사체심도가 소수시력에 주는 영향은 일정하였으나, 회절이 급격히 증가하여 정시에 대해 계산한 시력은 동공크기 1.5 mm 이하에서 감소했다. 근시는 회절과 수차보다 등가구면 굴절이상의 영 향이 커서, 동공크기 1.0 mm까지 계산한 시력이 직선적으로 증가했다. 근시성 고도난시(C= -2.73±0.63 D), 근시성 약도난시(C= -1.93±0.39 D), 원시성 난시(C= 1.29±0.72 D)에서는 시력의 변화가 등가구면 굴절이상의 크기가 유사한 근시 또는 원시와 같았다. 결 론: 핀홀의 크기가 감소할 때, 정시와 원시는 수차가 감소하지만, 회절이 증가하여 소수시력이 약간 감소하였다. 피사체심도가 소수시력에 미치는 영향은 핀홀크기에 관계없이 일정하였다. 근시는 등가구면 굴 절이상의 영향이 커서 소수시력이 크게 증가하였다. 난시의 소수시력은 난시도보다 등가구면 굴절이상의 영 향을 주로 받기 때문에, 핀홀이 난시도를 개선했다고 할 수 없다.

The CRISPR/Cas9 system is proved to be a powerful tool for knock-out and knock-in in various species. By introducing genetic materials of two components (Cas9 and small guide (sg) RNA) into cells or pronuclear of the fertilized embryo, gene editing occurs. Some studies reported that efficiency of gene editing would be increased as Cas9 was integrated into cells or animals since Cas9 is indispensable in the CRISPR/Cas9 system. Accordingly, the production of Cas9 expressing cattle may provide the broadly used gene editing platform in cattle. For this study, Cas9 and RFP genes were cloned into PiggyBac (PB) transposon system. PB-Cas9-RFP and transposase were microinjected into 1436 in vitro fertilized embryos and 241 blastocysts were formed. Blastocysts with RFP expression accounting for 14.1% of total formed blastocysts were selected and transferred into 5 recipient cow. After gestation periods, four transgenic cattle were delivered without any veterinary assistance. From a transgenic cattle, ear skin tissue was collected for primary culture. On those primary cells, sgRNAs in DNA form for various genes such as PRNP, RB1 and BLG were transfected as 2ug of sgRNA per 5x105 cells using Nucleo factor system (Neon®, invitrogen, program#16). As expected, every group of each sgRNA delivered was confirmed to be mutated by T7E1assay. Those data demonstrated that for the first time, transgenic cattle with Cas9 expression were born, grown up to date and will be avaluable resource for genome-editing in cattle. This work was supported by BK21PLUS Program for Creative Veterinary Science and Seoul Milk Coop (SNU550-20160004).

This study examines whether revision mediates the effects of its preceding written corrective feedback (CF) in developing second language (L2) knowledge. For this purpose, effects of direct CF and three sub-types of metalinguistic CF with and without revision were compared in the quasi-experiment with 197 EFL university students. They formed 9 groups (i.e., 4 revision groups, 4 non-revision groups, and 1 control group) and completed 4 dictogloss writings. Written CF and revision were implemented after the first two writings. English articles were the target structure for the written CF. The first, third and fourth writings were measured for the accuracy of article use as the pre-test, the immediate post-test and the delayed post-test, respectively. The results reveal that revision did not affect the efficacy of direct CF while it enhanced that of metalinguistic CF to varying degrees with its subtypes. These findings indicate that, if revision is conducted following written CF with particular metalinguistic information, it may facilitate learning new L2 knowledge beyond manifesting existing L2 knowledge.

This research aims to look at the contradictory effects of corporate social responsibility as conducted by luxury brands. On the one hand, corporate social responsibility (CSR) is known to product positive effects on brands such as the transfer of goodwill and image but on the other hand because of the purported impact of disfluency, previous research has argued that the emphasis on self enhancement runs counter to the more social agenda of CSR. In this study we examine of power and how that can mediate when positive and negative impact of CSR when such campaigns are implemented by luxury brands.

The high temperature deformation behavior of Ni3Al and Ni3(Al,Mo) single crystals that were oriented near <112> was investigated at low strain rates in the temperature range above the flow stress peak temperature. Three types of behavior were found under the present experimental conditions. In the relatively high strain rate region, the strain rate dependence of the flow stress is small, and the deformation may be controlled by the dislocation glide mainly on the {001} slip plane in both crystals. At low strain rates, the octahedral glide is still active in Ni3Al above the peak temperature, but the active slip system in Ni3(Al,Mo) changes from octahedral glide to cube glide at the peak temperature. These results suggest that the deformation rate controlling mechanism of Ni3Al is viscous glide of dislocations by the <110>{111} slip, whereas that of Ni3(Al,Mo) is a recovery process of dislocation climb in the substructures formed by the <110>{001} slip. The results of TEM observation show that the characteristics of dislocation structures are uniform distribution in Ni3Al and subboundary formation in Ni3(Al,Mo). Activation energies for deformation in Ni3Al and Ni3(Al,Mo) were obtained in the low strain rate region. The values of the activation energy are 360 kJ/mol for Ni3Al and 300 kJ/mol for Ni3(Al,Mo).

Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for detecting antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 5-h treatment of samples by placing them in water dosed with sulfadimethoxine (SDM; 200 g/ton water), the residue depletion of SDM was investigated in 25 cultured olive flounders (Paralichthys olivaceus). Muscles from fish were sampled before treatment and on the 1st, 2nd, 3rd, 4th and 5th days after treatment. The concentration of SDM in the muscle was then determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the residue in olive flounder muscle. To investigate the recovery rate, standard solutions were added to muscle samples to obtain final concentrations of 25 and 50 ng/mL in the muscle. The recovery rates of all spiked samples were >96.6% of the spiked value. SDM was detectable in the muscle of fish treated with the drug until the 1st day of the withdrawal period. The present study shows that the LFIA can be easily adopted to detect SDM residues in the tissue of farmed fish.

This study investigated the antibacterial effects of Galla rhois extract (GRE) against Campylobacter jejuni and Campylobacter coli. The minimum inhibitory concentrations (MICs) of GRE against C. jejuni and C. coli were 0.28 and 0.55 mg/mL, respectively, and the corresponding minimum bactericidal concentrations (MBCs) were 4.4 and 5.5 mg/mL. C. jejuni treated with the MIC, MBC or 2×MBC of GRE showed significant inhibition of growth compared with that of the control group during the incubation period, and no viable bacteria were detected at 24 h after incubation. C. coli treated with MIC, MBC or 2×MBC of GRE also showed inhibition of growth compared with that of the control group during the incubation period, and in the C. coli cultures treated with MBC and 2×MBC of GRE, no viable bacteria were detected at 24 h after incubation. In conclusion, GRE is a candidate antibacterial agent against C. jejuni and C. coli, and may have applications for the control of Campylobacter infection in poultry.

For the fabrication of the Si negative electrode in Li-ion batteries (LIBs) containing the cross-linking polymer binder, in this work, the urethane acrylate (UA) oligomer was synthesized via a simple synthetic process. The cross-linked poly(urethane acrylate) (CPUA)/carbone black (CB)/Si composite (CPUA/CB/Si composite) was fabricated through reactions between their reactive vinyl segments in the UA oligomer. Interestingly, the CPUA/CB/Si composite showed better cycle performance than the poly(vinylidene fluoride) (PVdF)/CB/Si composite (PVdF/CB/Si composite) and the polyurethane (PU)/CB/Si composite (PU/CB/Si composite). The CPUA/CB/Si composite had the best lithiation of about 2586 mAh g-1. The UA oligomer showed a good compatibility with the electrode materials and current collector after and before a curing process.