In the field of logistics, the maritime shipping industry plays a critical role as the backbone of global trade activities. Nevertheless, previous studies on the commercialization and benefits of blockchain technology are limited in the field of marine logistics. Thus, the purpose of this study was to predict the benefit for each group involved in marine logistics when blockchain technology is applied. As such, 21 factors of benefits were selected for seven major logistics groups (financial institutions, freight forwarders, inland transportation, ocean carriers, port operators, port–related government authorities, and shippers) to study the benefit expected for each through the commercialization of blockchain technology. Based on the results, a different benefit level is expected for each group when blockchain technology is used. In order, ocean carriers (0.155), inland transportation (0.150), financial institutions (0.153), port operators (0.145), freight forwarders (0.142), port-related government authorities (0.129), and shippers (0.126) were found to benefit most from the use of blockchain technology. This study has industrial implications in that it presents the benefits expected when blockchain technology is realized and used in marine logistics by groups involved in logistics transactions.

최근 들어 VR 산업의 성장을 위한 양질의 VR 콘텐츠에 대한 필요성이 꾸준히 제기되고 있다. 이에 본 연구는 VR 콘텐츠 중에서 가장 큰 주목을 받고 있는 VR 게임의 사용자의 관심요소에 대해 연구하였다. 연구 수행을 위해 스팀(STEAM)의 사용자 리뷰 데이터를 활용하였고 리뷰 데이터에 텍스트마이닝과 네트워크 분석을 적용한 결과 VR 게임 사용자의 관심요소는 '현존감', '1인칭 시점 게임', '청각적 요소', '상호작용' 으로 확인되었다. 본 연구는 양질의 VR 게임 개발을 위한 사용자 관점의 연구를 수행하고 사용자 관점의 연구를 리뷰을 통해 시도한 초기 연구라는 것에 대해 그 의의가 있다.

The purpose of this study is to investigate the differences fatty acids and hormonal parameters in the spawning and non-spawning season between the diploid and induced triploid Far Eastern catfish, Silurus asotus. The measured triploids were produced by cold shock for 50 min at 4℃ in May 2014, the spawning season of diploid was in May, and the nonspawning season was designated in January. Estradiol and testosterone and gonadosomatic index of diploid were higher than those of induced triploid in spawning season (P<0.05), and those of diploid in spawning season were higher than nonspawning season. On the other hand, thyroid stimulating hormone and thyroxine of induced triploid were higher than those of diploid in spawning season (P<0.05). Erythrocyte count of diploid was higher than that of induced triploid in spawning season and non-spawning seasons. Mean corpuscular volume and mean corpuscular hemoglobin of induced triploid were higher than those of diploid in both seasons (P<0.05). Percentages of total saturated fatty acids and n-3 polyunsaturated fatty acids of induced triploid were higher than those of diploid in spawning season, but those of diploid were higher in non-spawning season (P<0.05). Percentages of total mono unsaturated fatty acids and total n-6 polyunsaturated fatty acids of diploid were higher than those of induced triploids in spawning season, while those of induced triploid in non-spawning season were higher (P<0.05). Therefore, induced triploids in the spawning season tend to concentrate on growth and lipid-synthesization, whereas, diploids concentrate on reproduction and gonadal maturation rather than on growth. In non-spawning season, growth and lipid- synthesization were not significantly different between diploid and induced triploid.

본 논문에서는 게이미피케이션 개념이 브랜디드 콘텐츠에 적용된 사례를 연구한다. 게이미피케이 션은 게임 요소를 비게임 분야에 적용하여 해당 분야의 효율을 높이는 개념이며 교육, 마케팅, 의료, 스포츠, 쇼핑 등의 분야에서 활발히 활용되고 있다. 특히 마케팅(광고)을 엔터테인먼트 콘텐츠로까 지 확장시킨 브랜디드 콘텐츠에서도 게이미피케이션 개념을 적용한 사례를 볼 수 있다. 이에 본 연 구는 게이미피케이션 개념이 마케팅에 어떠한 방식으로 영향을 끼치는지, 게이미피케이션이 브랜디 드 콘텐츠가 엔터테인먼트 콘텐츠로 확장하는데 있어 어떻게 작용하는지를 고찰한다. 이를 위해 게 이미피케이션과 브랜디드 콘텐츠의 개념과 특성을 살펴보았으며 5가지 실제 사례에 게임의 요소(목 표, 경쟁, 피드백, 보상)가 어떤 방식으로 적용되었는지 분석하였다. 최종적으로 브랜디드 콘텐츠가 엔터테인먼트 콘텐츠로 확장함에 있어 게이미피케이션이 효율적인 방식이 될 수 있음을 검증하였다.

Safflower (Carthamus tinctorius L.) seeds have long been clinically used in Korea to promote bone formation and prevent osteoporosis. In addition, the safflower buds (SB) were found to have more useful functional ingredients than safflower seed. Thus, we investigated the preventive effects of SB diet in ovariectomized (OVX) rats. The rats were divided into five groups; sham operated group, OVX alone group, OVX plus 17β-estradiol (E2 10 ㎍/㎏, i.p.) and OVX plus SB diet feeding group (0.3% or 1%). Feeding of SB diet (0.3% or 3%) to OVX rats markedly increased trabecular formation in femur compared to OVX rats. Feeding of SB diet (0.3% or 3%) to OVX rats also decreased TRAP activity compared to OVX rats. These results suggest that SB diets have bone sparing effects by the decrease of osteoclast activity. We also observed that OVX rats fed with SB diet (0.3% or 3%) exhibited the decrease of calcium and phosphorus in serum compared to OVX-induced rats. Therefore, SB may be beneficial for the patients of osteoporosis, especially in postmenopausal women.

Fusarium crown root rot (FCRR) is a severe fungal disease caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL) in tomato. Resistance to FORL is conferred by single dominant locus Frl on chromosome 9, but its precise genomic location is not clearly determined. In this study, detailed location of Frl was assessed by using a set of molecular markers physically anchored on Chr.9 and F2 and RIL population derived from FORL-resistant inbred AV107-4 (S.lycopersicum) x susceptible L3708 (S. pimpinellifolium). Bioassay of the two populations with a FORL strain isolated from Korea resulted in single dominant heritance of the resistance. Two SCAR and 11 CAPS markers encompassing 3.6Mb~72Mb of Chr.9 were developed from the Tomato-EXPEN 2000 map and SolCAP SNP-array analysis. These markers were genotyped on 345 F2 plants. A high level of cosegregation with the resistance were observed for 5 markers which were mapped at a large physical interval of 5.1Mb (T1212) to 46.4Mb (SSR237), indicating that genetic recombination was highly suppressed in this region. Cosegregation of these markers with Frl was confirmed by using 126 RILs. The results implied that, in contrast with the previously reported long arm, Frl is present on a pericentromeric region of short arm of Chr. 9, in which crossing-over is severely suppressed. The marker set was further tested on 12 FORL-resistance or susceptibility commercial cultivars. Unlike the biparental populations, frequent linkage break was observed for T1212 and D4 in commercial cultivars. T1212 and D4 showed 50% and 100% match with the phenotype, respectively. D4, a CAPS, was converted to a high resolution melting (HRM) marker and tested on 55 breeding lines from private seed companies (Fig.3). All breeding lines showed the HRM genotype for resistance allele, indicating that D4 can be useful for selecting FORL-resistance tomato plants.

본 연구에서는 모바일 게임 이용량(이용시간, 소비비용)이 게임의 지각된 만족도(지각된 즐거움, 서비스 품질, 가격)와 몰입에 미치는 영향을 탐색하고자 한다. 게임 이용량이 많을수록 게임에 대한 만족도가 높고, 게임에 몰입하는가? 게임 몰입에 있어 영향을 끼치지 않거나, 부(-)의 영향을 끼치는 요인은 없는가? 연구 결과 본 논문에서 상정한 모바일 게임 이용 행태 중 이용시간과 소비비용의 정도가 높을수록 게임에 대한 만족도와 몰입의 정도가 증가함이 밝혀졌다. 본 연구는 상대적으로 모바일 게임 이용량이 높은 이용자가 모바일 게임에 대한 지각된 만족도와 몰입의 정도가 높음을 실증적으로 분석함으로써 모바일 게임 중이용자, 나아가 디지털 게임 중이용자 연구 저변의 확대 및 담론에 참여하고자 한다.

Anthocyanins are very important constituent of human diet. In recent years, they have gained much attention due to their antioxidative properties. As the radish (Raphanus sativus L) has rich content of anthocyanins, the study is aimed to develop increased functional radish from selected radish varieties. ‘Bordeux’ is a hybrid variety of breeding between one accession derived from ‘Oharu’ as a maternal variety and ‘Chungpihongsim’ as a paternal variety. In this study, we investigated the new varieties of radish commonly consumed in Republic of Korea for their total phenolic content (TPC) and antioxidant activities using three (DDPH, FRAP and CUPRAC) different assays. The selected radish varieties like ‘Bordeux’, ‘Chungbok’, ‘3209’, ‘Chungwoon’, ‘Oharu’, and ‘Chungpihongsim’ were procured from the company Syngenta Korea. Among the selected radish varieties, ‘Bordeux’ (289 μg/g FW) and ‘Chungpihongsim’ (276 μg/g FW) revealed maximum amount of phenolics; whereas ‘3209’ (103 μg/g FW) and ‘Chungwoon’ (166 μg/g FW) showed the lower amount of phenolics content, respectively. Extracts from these studied radishes showed good to moderate antioxidant activities. The varieties ‘Chungpihongsim’ and ‘Bordeux’ revealed maximum antioxidant activity for all assays as demonstrated. However, some varieties like ‘3209’ and ‘Oharu’ exhibited the lowest antioxidant activity in all the tested assays, viz; DPH, FRAP and CUPRAC in μg TE/g FW, respectively. The antioxidant activities may be attributed to the higher phenolic acid contents as a linear relation was observed between the two components and the antioxidant parameters.

The inflammatory response to infections, such as bacteria and viruses is mediated by multiple host factors. The tumor related-genes are the important cytokines in mammals. However, a number of tumor related-genes are not identified in the rock bream. Here, we have reported the identification and molecular characterization of the tumor related genes. The LPS-induced TNF-α factor 1 and 2 (LITAF1, LITAF2), tumor necrosis factor superfamily member 14 (TNFSF14), tumor necrosis factor receptor superfamily member 14 (TNFRSF14) and translationally controlled tumor protein (TCTP), programmed cell death 10 (PCD10) from rock bream are used for the under investigations. The LITAF1 and LITAF2 consist of 138 and 163 amino acids with a conserved LITAF domain. TNFSF14 and TNFRSF14 comprise 266 and 181 amino acid, respectively. TCTP encompasses of 170 amino acid containing two conserved TCTP signatures. Furthermore PCD10 consists of 210 amino acids. Using quantitative real-time PCR, we have obtained expression analysis results of LITAF1 and 2, TNFSF14, TNFRSF14, TCTP, PCD10 in the various tissue. Compared to the control, the tumor related genes mRNA is detected at a high levels in gill (LITAF1, TCTP), intestine (LITAF2), liver (PCD10), spleen (TNFSF14) and RBC (TNFRSF14). We have also performed gene expression analysis in the kidney, spleen, liver and gill after challenging with Streptococcus iniae, Edwardsiella tarda and Red seabream iridovirus. We have acquired the dynamic regulated mRNA expression to each of pathogen according to the tissue. Expression of tumor related-genes mRNA are significantly increased by infected with pathogens in most of the tissue. But oddly, PCD10 mRNA is expressed significantly decreased by S. iniae infection in all of tissues. Our results reveal that rock bream tumor relatived-genes may be involved in rock bream immune responses to pathogen infections, as well as, they also act like potential biomarkers for innate immunity.

Translationally controlled tumor protein (TCTP) is one of important immune regulator. TCTP has been implicated in cellular processes including the cell growth, cell cycle progression, apoptosis regulation and the protection of cells against various stress condition. In this study, we cloned and characterized TCTP from rock bream (Oplegnathus fasciatus), which is an economically important species in the Korea aquaculture industry. The Full-length of rock bream TCTP (RbTCTP) cDNA was 1041 bp and contained an open reading frame (ORF) of 513 bp, which encoded 170 amino acid sequence. The 5' untranslated region (UTR) was 90 bp while the 3' UTR was 538 bp, containing a polyadenylation signal (ATTAAA). The identity of amino acid sequence was 76%, 75% and 74% in tilapia, orange-spotted grouper and Japanese seaperch, respectively. The positions of microtuble-binding region, Ca⁺ binding region and TCTP signature regions in RbTCTP were similar with those of other fish species and mammalian. The RbTCTP mRNA was expressed highest in the muscle. Expression of TCTP mRNA were significantly variable according to injection of red seabream iridovirus (RSIV), Streptococcosis (S. iniae) and Edwardsiella tarda (E. tarda).