Recently, as the internet usage is increasing, accordingly generated text data is also increasing. Because this text data on the internet includes users’ comments, the text data on the Internet can help you get users’ opinion more efficiently and effectively. The topic of text mining has been actively studied recently, but it primarily focuses on either the content analysis or various improving techniques mostly for the performance of target mining algorithms. The objective of this study is to propose a novel method of analyzing the user’s requirements by utilizing the text-mining technique. To complement the existing survey techniques, this study seeks to present priorities together with efficient extraction of customer requirements from the text data. This study seeks to identify users’ requirements, derive the priorities of requirements, and identify the detailed causes of high-priority requirements. The implications of this study are as follows. First, this study tried to overcome the limitations of traditional investigations such as surveys and VOCs through text mining of online text data. Second, decision makers can derive users’ requirements and prioritize without having to analyze numerous text data manually. Third, user priorities can be derived on a quantitative basis.

본 연구는 학교 장면에서 학생지도 시 교사의 상업용 온라인 게임 활용 의사에 영향을 미치는 요인이 무엇인지, 그리고 각 요인들이 어떠한 영향을 미치는지를 알아보기 위해 수행되었다. 이를 위해 면대면 설문조사를 실시하였고, 전국 초, 중, 고등학교 교사와 상담교사, 전문 상담사, 총 236명의 자료가 분석되었다. 교사의 인구통계학적 특성과 교사의 상업용 온라인 게임 활용 의사의 관련성을 분석해본 결과, 남성보다 여성교사가, 교사의 연령이 높고 근무 기간이 길수록 상업용 온라인 게임 활용 의사가 낮은 것으로 확인되었다. 교사의 상업용 온라인 게임 활용 의사에 영향을 미치는 요인을 중다회귀분석방법을 통해 분석한 결과, 교사의 개방성과 디지털 게임에 대한 태도 중 디지털 게임의 유익성에 대한 인식, 디지털 미디어 활용능력이 학생지도 시 교사의 상업용 온라인 게임 활용 의사에 정의 영향을 미치는 것으로 확인되었다. 본 연구는 교과학습 및 학생 생활 지도에 있어 교육적 잠재력을 가지고 있는 상업용 온라인 게임에 대한 교사의 활용 의사를 촉진하는 주요 요인들을 밝혔다는데 그 의의가 있다.

최근 기능성 게임의 교육적인 활용에 대한 관심이 증가하면서, 게임의 교육적 효과성에 대한 많은 연구들이 이뤄지고 있다. 본 논문에서는 인지기능 향상을 위한 기능성 게임인 ‘바우바우 프렌즈’를 개발했다. 이 콘텐츠는 인지기능 중 집행기능(Executive function), 지속적 주의력 (Sustained attention), 분리적 주의력(Divided attention)을 다루고 있다. 본 연구에서 개발된 게임의 유용성을 증명하기 위해 EEG 실험을 수행했으며, 게임 전 뇌파와 게임 진행 중 뇌파를 측정하여 비교분석했다. 결과적으로 지속적 주의력 게임(Simon)과 분리적 주의력 게임(Cooking)이 인지기능 향상에 긍정적인 효과가 있는 것으로 나타났다. 보다 아동의 인지기능 향상이라는 명확한 검증을 위해서는 추후 아동을 대상으로 한 EEG 실험을 추가로 수행할 필요가 있다.

Acinetospora filamentosa and Microspongium stilophorae are reported as new records from South Korea based on morphological and molecular analyses. A. filamentosa is mainly characterized by having the sparsely branched erect filaments, the scattered meristematic zones, forming crampons, plurilocular sporangia on both prostrate filaments and lower part of erect filaments, and spherical to oval unilocular sporangia formed either sessile or with a pedicel. M. stilophorae is an epiphytic thalli mostly on Stilophora sp. It is characterized by prostrate filaments with irregular cells, short erect filaments with short ramuli, phaeophycean hairs, uniseriate plurilocular sporangia on the terminal part of erect filament. Our molecular analyses of rbcL and cox1 genes reveals that A. filamentosa and M. stilophorae are nested within the clades of Acinetospora and Microspongium, respectively.

Feldmannia chitonicola is reported as a new record from Korea based on morphological studies in laboratory-cultured materials and molecular analyses. F. chitonicola is mainly characterized by a small size (1-2 mm), erect filaments mostly unbranched, plurilocular sporangia produced on both prostrate and laterally on the basal part of erect filaments, and 2 types (lanceolate and ovate) of sporangia. In our cultures, sporangia production was slower at 10°C than in 16°C and 20°C. Our molecular analyses of rbcL and cox1 genes supported its independence from other congeners reported for Korea. This is the first report of F. chitonicola for western Pacific.

Honeybees are inevitably threatened by various pathogens including Sacbrood virus (SBV) and Galleria mellonella. Recently, RNA interference (RNAi) has been suggested as a promising strategy for suppression of honey bee viruses. Also, Bacillus thuringiensis (Bt) has been widely applied for the control of lepidopteran pests such as G. mellonella. In this study, it was intended to develop dsRNA production platform using Bt. For this, the pHT1K-SBV vp1 vector which transcribes sense and anti-sense SBV vp1 gene under control of Cyt1Aa sporulation-dependent promoter was introduced into Bt strain NT0423 expressing Cry1-types toxins. SBV replication was suppressed in the worker A. cerana ingested dsRNA produced from the Bt transformant. Crystal proteins from the Bt transformant showed high level of insecticidal activity against 4th instar larvae of G. mellonella.

Mosquitoes are medically important insect pests that act as vectors of various diseases when they feed on humans. Insect growth regulators (IGRs) could become an effective alternative to control mosquitoes because of their high specificity and relatively low toxicity to environment. Recently, we have developed high-throughput juvenile hormone antagonist (JHAN) screening system based on yeast-two hybrid assay. Actinomycetes have been reported to produce various bioactive compounds including insect growth regulators (IGRs). In this study, culture filtrates and mycelia extracts of 2,875 actinomycetes isolates screened for their IGR activities and mosquitocidal activities. Among 75 culture filtrates and 17 mycelia extracts with JHAN activities, 19 culture filtrate and 3 mycelia extracts showed high level of mosquitocidal activities against 3rd instar larvae of Aedes albopictus. These results suggested that secondary metabolites of actinomycetes could be used as environmentally benign mosquitocidal agents.

In order to investigate novel insect growth regulators (IGRs) from actinomycetes, 363 isolates of actinomycetes were tested for their IGR activities. Among them, Streptomyces sp. AN120537 showed the highest juvenile hormone antagonist (JHAN) activity and significant insecticidal activities against larvae of Aedes albopictus and Plutella xylostella. In addition, the dead larvae showed morphological deformities such as contraction of body segments or pigmentation of all body parts. Through liquid chromatography and bioassay-guided fractionation, 7 IGR compounds were identified from the crude hexane extract of AN120537. These results suggested that Streptomyces sp. AN120537 could be useful resources for development of eco-friendly insecticidal agents.

We investigated developmental characteristic of life cycle of the lawn cutworm, Spodoptera depravata (Lepidoptera: Noctuidae), which is one of the most economically damaging pests to grasses. For larval culture the turfgrass, Zoysia japonica (Poales: Poaceae), was provided at 25℃ and 60 ± 5% humidity. Developmental period of each stage was as follows: 4.11 ± 0.19 days for eggs, 25.17 ± 3.02 for larvae, 8.80 ± 0.28 for pupae and 7.57 ± 0.95 for adults. Larvae was grown to 7th instar, unlike pre-existing data, which indicated the final age as the 6th instar. Body length of larvae changed on average from 2.772 mm immediately after hatching to 2.3 cm at 7th instar and head capsule width changed on average from 0.423 mm at 1st instar to 2.82 mm at 7th instar. Pre-period of eggs deposition was 2 days, oviposition period was 4.6 days, average fecundity per female was about 419 eggs, and hatching rate was about 83%.