Anthriscus sylvestris (L.) Hoffm. is a perennial herb found widely distributed in various regions of Korea, Europe, and New Zealand. The root of A. sylvestris have been extensively used in the treatment for antitussive, antipyretic, cough remedy in Oriental medicine, but the physiologically active function of the leaf of A. sylvestris is as yet unknown. In this study, we investigated the anti-cancer activity and the mechanism of cell death of water extracts of leaf of Anthriscus sylvestris (WELAS), on human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that WELAS treatment inhibited cell viability in a concentration- and time-dependent manner. In addition, the treatment of WELAS markedly induced apoptosis in FaDu cells, as determined by the viability assay, DAPI stain and FACS analysis. WELAS also increased the proteolytic cleavage of procaspase-3, -9 and PARP (poly(ADP-ribose) polymerase). In addition, exposure to WELAS decreased the expression of Bcl-2 (an anti-apoptotic factor), but increased the expression of Bax (a pro-apoptotic factor), suggesting that mitochondria-dependent apoptotic pathways are mediated in WELAS-induced apoptosis. Taken together, these results indicate that water extracts of leaf of A. sylvestris inhibits cell growth and induces apoptosis via the mitochondrial-dependent apoptotic pathway in FaDu human hypopharyngeal squamous carcinoma cells. Therefore, we propose that the water extracts of leaf of A. sylvestris is a novel chemotherapeutic drug, having growth inhibitory properties and induction of apoptosis in human oral cancer cells.
Evaluation of the antioxidant activity of the bakery products is important in order to establish healthy consumption patterns. Aronia and blackberry extract at different levels (0%, 5%, 10%, 15%, and 20%) were used to investigate their comparative effects on the physical properties and antioxidant capacity of cookies. The final cookies were evaluated for physicochemical and sensory properties as well as total phenol content, DPPH radical scavenging activity, and FRAP assay. Substitution of aronia and blackberry extracts significantly (p<0.05) increased the moisture content and spread ratio compared to the control that did not contain the extract. However, types of the extract (aronia or blackberry) did not significantly affects the moisture and spread ratio of cookies at the same concentrations (p>0.05). The lightness and yellowness values of cookies were decreased while the redness value was increased by the amount of the extract compared to control (p<0.05). For textural properties, the hardness and chewiness of cookies were increased by the concentration of extract, and highest at 20% of both extracts. Polyphenol content in the cookies was increased with the concentration of the extract added into the cookies. Consistently, the antioxidant activity was significantly increased as the concentration of the extract was increased (p<0.05). Cookies with aronia extract showed higher antioxidant activity compared to that with blackberry (p<0.05), due to the presence of higher content of polyphenol. In terms of appearance, flavor, taste, texture and overall acceptability, the sensory evaluation scores of cookies with 20% aronia and 10% blackberry were significantly higher compared to the control (p<0.05). Based on above result, using more than 10% of the aronia or blackberry extracts would appropriate for making cookies.
For the development of processed food for hypoglcemia, it is important to construct model system to confirm factors that reduce the glycemic index in real food. This study was to investigate the relationship between the gel model system and real food the high amylose type of segoami using response surface methodology. The independent variables were concentration (X1; 10, 15, 20, 25, and 30%) and steaming time (X2; 30, 35, 40, 45, and 50 min). The predicted glycemic index (Y1) was analyzed as a dependent variable. The regression of pGI was 0.7343, indicating that the model fits the data well in the prediction test. The predict glycemic index of the gel was in the range of 71.38 ~ 83.78, depending on the gel preparation conditions and predicted optimum condition was 23.7% gel concentration and 43.8 minutes of gelatinization. The predict glycemic index of rice gel were decreased with an increase of gel concentration, rather than the steaming time. In order to confirm the effect on in vitro digestibility in real food, the real food (Garraedduk) was prepared by applying the optimal conditions. As a result, there was no significant difference between the predicted value (77.1) and the experiment value (76.6). This result showed that the in vitro digestibility in real food can be predicted by applying the gel model system.
Dermanyssus gallinae is parasitic to chicken, and they cause many damages such as disturbing sleep reducing for body weight and egg production by blood-sucking. To develop acaricide against D. gallinae, The acaricidal activity of 40 species plant extract were examined. Cnidium officinale extracted by Me-OH showed 82.0% acaricidal activity after treated 48 hrs at 4,000 ppm. The hexane fraction showed 92.4% mortality against D. gallinae at 48 hrs at 2,000 ppm. Purification of the biologically active constituents from the hexane extraction with acaricidal activity was done using silica gel open column chromatography and HPLC. H1122 fraction gave 80.9% mortality to D. gallinae at 400 ppm after treated 48 hrs. H1122 fraction was analyzed by GC-MS and NMR.
This study investigated the antidiabetic effect of amaranth grain ethanol extract (AEE) in a diabetic animal model, db/db mouse. The mice were divided into 4 groups: normal control mice (C57BL/6J), diabetic mice (C57BL/6J db/db), diabetic mice fed a lower concentration of AEE (0.3 mg/kg), and diabetic mice fed a higher concentration of AEE (0.5 mg/kg). After 10 weeks of treatment, body weights, blood insulin levels and blood glucose levels of each group were compared. At the end of treatment, the results showed that both AEE supplemented groups had lower body weights than those in the diabetic groups although higher than those in the normal groups. Moreover, in both AEE supplemented groups, serum insulin levels were higher and blood glucose levels were lower than those in the diabetic groups although both values were higher than those in the normal groups. The results of this study suggest that AEE can alleviate many of the common symptoms of diabetes in diabetic mice and, therefore, has potential as a therapeutic supplement for normalization of blood glucose and insulin levels in humans.
The aim of this study was to investigate the effect of Maitland mobilization and Kaltenborn-Evjenth mobilization on the SLR angle. Subjects randomly divided into Kaltenborn-Evjenth group(n=8) and Maitland group(n=7). The mean height, age, body weight was 176.00±5.10 cm, 22.75±1.83 years, 72.63±10.65 kg respectively in Kaltenborn-Evjenth group. The mean height, age, body weight was 175.00±5.60 cm, 22.29 ±3.68 years, 78.00±12.36 kg respectively in Maitland group. Hip joint accessary movements with Grade Ⅲ or IV were applied depend on the patient’s condition to the restricted direction for 1 minute each set, and performed 5 set in a Maitland group. Hip joint anteroposterior gliding with Grade Ⅲ were applied 60 for 1 minutes each set, and performed 5 set in a Kaltenborn-Evjenth group. The angle of first pain was referred to as P1 and subjects were pointed out that they could not bend the knee anymore, then examiner measure SLR angle. The SLR was significantly increased in the Maitland group compared to the Kaltenborn- Evjenth group after intervention(p<.05). In a within group difference, SLR significantly increased in the both groups(p<.05). These results indicated that Maitland mobilization could be recommended the excellent technique to increase the hip flexion in patient with hip hypo-mobility.
Zika virus, mosquito-borne disease, caused by mosquitoes has been increased the importance. From March to September, twice a month from 7 different points (3 residential areas, 3 migratory bird sanctuary and 1 cattle shed) were collected using BG trap and BL trap. After identifying the mosquitoes collected, we confirmed the virus infection. Total 26,531 mosquitoes (6 genus 9 species) were collected, virus has been detected from the 3 species (Aedes vexans, Cuilex tritaeniorhynchus, and Amigeres subalbatus) of mosquitoes of them. It showed the highest peak in August, and then gradually decreased. The most common mosquito species was collected Aedes vexan (16,637) in the cattle shed.
This studies was conducted to compare functional diversity of terrestrial arthropods in commercial apple orchards under conventional and organic practices. We collected terrestiral arthropods using pitfall traps in four conventional and seven organic apple orchards from April to October, 2012-2014. Sampled arthropods were identified at the species level and then classified three functional groups (detritivores, herbivores and beneficial arthropods included pollinators, parasitoids and predators). Biodiversity was analyzed with species richness, abundance and shannon index for each group and compared between conventional and organic orchards. In results, species richness of detritivores and benefical arthropods were higher in organic orchards than in conventional orchards (detritivores: t=-2.68, df=9, P=0.03; beneficial arthropods: t=-3.98, df=9, P=0.003). Organic orchards showed significant difference at abundance of beneficial arthropods (t=-3.33, df=9, P=0.008) and higher shannon index at detritivores (t=-2.36, df=9, P=0.04) than conventional orchards. However, all biodiversity indices of herbivores were not significantly different between conventional and organic orchards at 95% confidence level.
This study attempts to investigate the impact of entrepreneurial orientation on positive psychological and behavior intention such as psychological ownership, work engagement, organizational citizenship behavior, sales efforts, adaptive selling behavior, performance for internal marketing based on job demands and resources model (JD-R model). The purpose of this study is to examine how the five-dimensional entrepreneurial orientation (innovativeness, proactiveness, risk taking, autonomy, aggressiveness) can affect salespeople’s positive motivation and to suggest how to manage and train salespeople effectively as internal customers. For this study, data were gathered from the respondents who are salespeople and analyzed by using structural equation model (SEM). According to the results of the study, entrepreneurial orientation affected salespeople’s positive psychological ownership and work engagement. In addition, we confirmed that psychological ownership affected to increase work engagement and organizational citizenship behavior, and work engagement was a positive precedence factor increasing organizational citizenship behavior, sales efforts and adaptive selling behavior. Lastly, we found that organizational citizenship behavior, sales efforts and adaptive selling behavior showed a positive impact on performance. Based on the study results, this research suggested academic and managerial implications to manage and train salespeople effectively in the business-to-business markets.
Although expression of foreign genes in a crop species has been target techniques for powerful protection against insect pests, classical breeding programs using varietal resistance of a crop are still being processed. It is, however, frequently difficult to find key products expressed from resistance-related genes in the variety, and those action mechanism. Here, an unterminated story about seed of a mungbean (Vigna radiata) variety with high resistance against the adzuki bean weevil and the bean bug is introduced. The two insects cannot survive on seeds of the variety, although they well develop on susceptible ones. Molecular markers linked to the resistance were selected through BAC library screening and near isogenic lines, and finally a bruchid resistance gene was suggested after map-based cloning approaches. Starvation and chemicals were suggested for a resistant mechanism and a related factor through feeding and behavior experiments, respectively. The seed flour was extracted with organic solvents, and isolated into several fractions on chromatography. Several peaks on HPLC in a fraction were related with the high mortality of the bean bug. A partial structure in an isolated chemical was observed before full identification.
Many forest pests present a problem during wood quarantine. The effectiveness of phosphine (PH3), which is an altered form of MB, was examined in Monochamus saltuaris and M. alternatus. After 24 h, the larvae and the adults of both insects were susceptible to PH3 at 3 mg/L at 20°C. However, the larvae of both pests were rather than dead, seem to have been quiescent condition. PH3 showed increasing insecticidal activity in a time-dependent manner in the larva stage of both pests. We investigated the fumigants at all stages of M. saltuaris and M. alternatus, as well as the synergistic effects of PH3 in controlled atmospheres of 50% and 80% oxygen. The atmospheric oxidation of PH3 fumigation slightly increased the toxicity of the fumigant to larva stages in 125 L container at 4 mg/L.
These results indicate that highly concentrated atmospheric oxidation of PH3 could be useful as a fumigant agent against M. saltuaris and M. alternatus.
Calepitrimerus vitis (Nalepa) is a grapevine pest and distributed worldwide. This study was conducted to identify grapevine damage related to density of C. vitis and to suggest an effective control time. The damaged flower buds displayed retarded growth and became dried up, and symptoms of damaged leaves such as malformed and bronzed leaves were observed. Flower cluster and leaves were collected and density of the grape rust mite were observed by using a washing and sieving method. The highest mean density (±SE) of C. vitis was found to be 672.6±112.9 per flower cluster with mid-level damage, and 450.1±66.5 per 2 leaves was found on the damaged leaves. These severe damage symptoms were observed in the early spring, and the density of the grape rust mite was significantly higher as 80 times in damaged vineyard than in the undamaged at the early growth stages of the grapevine. To determine the emergence time of the grape rust mite from lower part of a winter bud the ratio of density of C. vitis on upper-bud to lower-bud was observed. On 13 April 2015, 80% of the grape rust mites were observed on upper-bud where the mite could feed at the bud burst stage. Therefore, once the symptoms of damaged flower clusters and leaves are observed, acaricides should be sprayed before inflorescence emergence, and at the early growth stage between bud burst and leaf development in following year. This would allow the decrease of the density of C. vitis in early spring, and the progression of severe damage could be prevented.
Backed by the rapidly-growing economy, China sends the most tourists to Korea. Accordingly, growing consumption of Chinese tourists is strongly drawing attention of the Korean retail industry. Academia has conducted more relevant researches, such as effective branding of Korean Wave, and services to attract tourists. Despite a series of efforts, including specialized tour products and language services, there have been an increasing number of Chinese visitors complaining about their experience in Korea and Chinese tourists are urgently demanding improvements. Hence, comprehending sophisticated consumption behavior of Chinese tourists is becoming more important than ever before. Also, it needs to explore how shopping experiential value that they experience while purchasing fashion goods in Korea affects their satisfaction of the store and product.
Previous literature has shown that perceived shopping value has a positive effect on customer satisfaction and behavioral intention (Mathwick et al., 2001). Previous domestic studies that targeted Chinese tourists have focused on investigating the shopping motivation and satisfaction (Ko, 2011) and market segmentation of Chinese tourist shoppers (Oh, 2014). Previous studies have approached this problem mainly from its cognitive aspect and few studies have focused on the emotional aspect during shopping. This study suggests that emotional behavior mediates the relationship between shopping experiential value and consumer attitude toward products and stores.
According to the Korea Tourism Organization, the ratio of male to female Chinese tourists visiting Korea changed from 69:31 in 1995 to 59:41 in 2005. In 2013, female Chinese tourists outnumbered males at a ratio of 41:59. The number of Chinese female tourists continues to increase. Females are known to be more involved in fashion consumption. Hence, this study is conducted on 550 Chinese female tourists who have shopping experiences during their visit to Korea. The ages of the respondents range from 20 to over 50 years old, with a mean age of 33.5 years. Their shopping experiential value is measured by visual appeal, crowding, economic benefits, and service excellence, and is expected to trigger emotions of entertainment and escapism. Variables including entertainment and escapism are measured using the Experiential Value Scale developed by Mathwick et al. (2001).
This study finds that three experiential values (visual appeal, crowding, and service excellence) have positive influences on entertainment. For example, customers feel greater entertainment when visual appeal, service excellence and/or the number of customers in the store is greater. The emotion of entertainment turns out to have positive impacts on both store satisfaction and product satisfaction. According to the examination of how shopping experiential values affect escapism, crowding and economic benefits have a positive impact, whereas visual appeal and service excellence have a negative impact on escapism. Previous studies define escapism as an emotion of which customers are absorbed by entertaining activities and satisfied with shopping. This study assumes that all the shopping experiential values may have a positive impact on escapism. Escapism is a status where customers are deeply occupied with shopping without thinking of anything as if they were in another world, and a much stronger emotion of entertainment than usual pleasure from shopping. This study finds that the higher visual appeal and service excellence tends to generate the lower the escapism. This may be attributable to the fact that the survey was examined only on Chinese tourists. The respondents were asked to recall the most memorable item among the fashion products that they purchased during their visit to Korea. Their shopping experiences varied widely from well-organized malls, like department stores and duty free shops, to crowded environments, like Dongdaemun, traditional markets and subway stores. As a result, different experiential values have different impacts on escapism. Economic benefits and crowding which are experienced during shopping help to absorb in shopping, and these are likely to have positive impacts on escapism. On the other hand, visual appeal and service excellence may have different influences on escapism according to the shopping environment that Chinese consumers experienced. For example, Dongdaemun or subway stores are less visually appealing than department stores or duty free shops, but trigger stronger sense of escapism. Because survey questions to measure service excellence include Chinese (language) proficiency of sales staff, it is expected that higher language proficiency level of sales staff tends to generate lower degree of escapism, the sense of feeling that one is indeed in foreign country like Korea, for customers. Escapism has negative impacts on both product and store attitudes. Escapism is a status of absorption in which customers are emotionally occupied with pleasant feelings, and purchases along with a high escapism are likely to be less rational and more impulsive. In particular, this study examines tourist shoppers, and the respondents evaluate products they purchased in another country after they return home, and this may have caused the negative attitude toward products and shops. In fact, some previous studies found that overly positive emotions have negative impacts on purchase behavior (Liljander &Standvik, 1997; Andrade, 2005).
This study confirms that the shopping experiential values perceived by Chinese tourists affect their product and store attitudes through the emotions of entertainment and escapism. It is particularly notable that this study verifies diverse roles of different emotions such as entertainment and escapism in the context of tourist shopping. This study employs empirical analysis on tourists, and provides practical implications including the importance of shopping experiential values for developing retail strategies.