The cotton bollworm, Helicoverpa armigera is one of the most polyphagous and cosmopolitan pest species in world wide including Korea. It also showed high level of resistance against various types of insecticide. To set the integrated resistant management program, first, we tried to identify the insecticide resistant mechanism via transcriptome analysis of coding and non-coding RNAs using 4 strains (Australian susceptible and resistant strains, Korean and Brazilian resistant strains, additional a sub-lethal dose insecticide treatment in Korean resistant strain). From the illumina based RNAseq data sets with genome information, some resistant involved detoxification genes and long non-coding RNAs were discovered. Second, following molecular markers were developed from resistant strain specific amino acid substitution from those candidate genes. Third, functionally identified genes’ markers were applied in field populations using some molecular diagnostic tools such as LAMP (loop mediated isothermal amplification). Now additional functional analysis is going on for these un-characterized candidate genes and long non-coding RNAs.

본 연구에서는 선박의 속력을 고려한 해상교통량 평가 방법을 제안하였으며, 이를 선박의 속력을 고려하지 않은 기존의 방법 과 비교하였다. 평가를 위하여 평택·당진항 10일간의 GICOMS 자료를 본 연구에 적용하였다. 그 결과 제안된 방법으로 평가된 환산교통량은 기존의 평가 방법에 비해 4.41(±0.99)배 증가하거나, 0.59(±0.04)배 감소하는 것으로 나타났다. 제안된 평가 방법을 적용한 각 시간대별 평균 해상교통혼잡도는 기존의 평가 방법 결과에 비해 1.43(±0.10)배 높게 나타났으며, 각 시간대별 최대 해상교통혼잡도는 1.62(±0.34)배 높게 나타났다. 해상교통혼잡도 최대 평가 결과인 피크타임 해상교통혼잡도는 선박의 속력 분포로 인하여 기존의 평가 방법과 다르게 평가됨을 확인하였다. 결과적으로 선박의 속력은 실용교통용량 평가 시 중요 값으로 적용되기 때문에 해상교통량을 평가할 때 선박의 속력 을 고려하여야 할 것으로 사료된다.

Background: The craniocervical flexion (CCF) exercise is one of the effective exercise in correcting forward head posture (FHP). However, some people with FHP achieve CCF with compensatory movements, for example, low cervical flexion using superficial neck flexors such as the sternocleidomastoid (SCM) muscle. No study has yet investigated whether a dualpressure biofeedback unit (D-PBU) method to prevent low cervical flexion would be helpful in performing pure CCF movement. Objects: The purpose of this study was to compare the effects of the CCF using D-PBU method and the traditional CCF method on the cross-sectional area (CSA) of the longus colli muscle (LCM) and the activity of SCM muscle in subjects with FHP. Methods: Twentyfour FHP subjects (male: 16, female: 8) were recruited for this study. All subjects performed CCF using two different methods: The traditional CCF method and the CCF using D-PBU method. The CSA of the LCM was measured via ultrasound, and surface electromyography was used to measure SCM muscle activity. Results: The change in CSA of the LCM was significantly larger during the CCF using D-PBU method (1.28±.09) compared with the traditional CCF method (1.19±.08) (p<.05). The SCM muscle activity using the CCF using D-PBU method (2.01±1.97 %MVIC) was significantly lower than when using the traditional CCF method (2.79±2.32 %MVIC) (p<.05). Conclusion: The CCF using D-PBU method can be recommended for increasing LCM activation and decreasing SCM muscle activity during CCF movement in subjects with FHP.

소나무재선충은 북미가 원산으로 일본, 중국, 한국을 비롯한 동북아시아의 소나무류에 막대한 피해를 주고 있으며 포르투칼과 스페인에도 발생하여 유럽 소나무류에도 큰 위협이 되고 있다. 소나무재선충은 매개충인 Monochamus속에 의해 새로운 기주로 옮겨지기 때문에 매개충을 방제하는 것이 가장 중요하다. 국내 소나무재선충 매개충은 솔수염하늘 소와 북방수염하늘소가 보고되어 있으며 이들을 방제하기 위하여 주로 유기합성농약을 항공 살포하거나 연막기를 이용하여 살포하고 있다. 하지만 유기합성농약의 대면적 살포는 산림생태계에 큰 위협요인이 되고 있다. 본 발표에서는 유기합성농약을 대체하기 위하여 소나무재선충 매개충의 집합-성페로몬 및 카이로몬 연구 그리고 이들 유인제의 현장적용 연구 및 추후 연구방향에 대해 소개를 하고자 한다.

소나무재선충병의 방제를 위한 나무주사는 살선충제를 사용하여 예방적인 측면에서 이루어 졌다. 본 연구는 소나무에 살충제를 나무주사하여 약제를 소나무 전체에 침투 이행시킨 후 우화한 매개충이 후식하는 과정에서 약제가 포함된 신초를 섭식하게 되면 소화중독으로 교미 및 산란 전에 살충시킴으로서 매개충을 방제하고자 실시하였 다. 이러한 살충제 나무주사 방법은 신초를 후식하는 하늘소류만을 소화중독에 의해 살충시키는 방법으로 기존의 항공방제에 비해 환경에 대한 영향을 최소화 할 수 있다. 살충제 나무주사에 가장 적합한 약제 선발을 위해 실내에서 Neonicotinoid계 살충제 7종을 각 2,000배로 희석한 후 솔수염하늘소에 2㎕를 경구투입하여 살충효과를 평가한 결과 Clotianidin SL, Thiamethoxam DC이 소화중독에 의한 살충효과가 가장 우수하였으며, 이들 살충제를 야외에서 소나무에 나무주사 후 가지에 망대를 설치하고 솔수염하늘소를 접종하여 살충력을 검정한 결과 Thiamethoxam DC가 살충력이 가장 우수한 것으로 판단되었다. 또한 현장적용을 위해 잣나무림에 나무주사후 산란유인목을 설치하여 무처리구와 비교한 결과 살충제 나무주사 시험지의 산란유인목에서는 산란이 이루어지지 않아 현장방제에 적용 가능성을 확인할 수 있었다.

도시림, 생활림, 가로수, 도시공원 등 국민들의 일상생활을 하는 구역이나 장소에 위치한 생활권 수목 식재지에서의 농약 살포는 농약 살포자의 농약 노출 문제뿐만 아니라 농약의 살포 후 생활권 수목과 접촉하는 불특정 시민들도 지속적인 농약 노출이 문제가 될 수 있다. 본 연구에서는 생활권 수목의 관리를 위해 관행적으로 가장 많이 사용 살포되는 살충제인 Fenitrothion을 회양목에 살포하고 일정시간별로 손 노출량, 잎 잔류량, 호흡 노출량을 측정 한 뒤, 위해성 평가 수식(MOS; margin of safety)을 이용하여 체중별 안전 노출시간을 분석하였다. 그 결과, 살포된 Fenitrothion의 손을 통한 전이량이 급격하게 떨어지고 호흡노출량이 측정되지 않는 48시간 이전 까지는 농약노출에 대한 주의가 필요할 것으로 판단되었다.

This study was conducted to evaluate the the efficacy of mulberry cake mixed diet on larval growth of Protaetia brevitarsis. As a result of adding 3, 5, and 10% mulberry cake to the fermented mulberry sawdust, the development period of the Protaetia brevitarsis larvae was 44.9days, 44.6days, and 41.8days, and the highest weight of larvae was 2.76g, 2.80g, and 2.89g, respectively. As a result of adding 3, 5, and 10% mulberry cake to the fermented oak sawdust, the development period of the Protaetia brevitarsis larvae was 46.7days, 41.6days, and 41.7days, and the highest weight of larvae was 2.65g, 2.65g, and 2.56g, respectively.

솔수염하늘소는 소나무재선충병을 매개하는 중요한 해충으로 성충의 방제를 위하여 주로 항공살포나 지상살포에 의한 살충제의 접촉독에 의한 방제가 시행되어 왔다. 본 연구는 살충제를 나무주사하여 소나무재선충병의 매개충인 솔수염하늘소를 방제하기 위한 살충제 선발을 위해 Neonicotinoid계 살충제 7종을 각 2,000배로 희석한 후 솔수염하늘소 에 2㎕를 경구투여하여 소화중독에 의한 살충효과를 평가하였다. 살충제 경구 투여 후 신경흥분에 의한 경련반응을 조사한 결과 30분 경과에서는 Clotianidin SL(100%), Thiamethoxam DC(73.3%)이 경련 비율이 높았으며 상대적으로 Dinotefuran SL(46.7%), Imidacloprid DC(0.0%)가 약제에 대한 경련반응이 낮게 나타났다. 반수치사시간(LT50)을 조사한 결과 Clotianidin SL은 수컷에서 54.7시간, 암컷에서는 85.6시간으로 나타났으며, Thiamethoxam DC은 암컷에서 70.1시 간, 수컷에서는 88.7시간으로 나타나 소화중독 효과가 가장 우수한 것으로 나타났다. 따라서 살충제 나무주사를 이용하여 솔수염하늘소의 방제에서는 Clotianidin SL, Thiamethoxam DC가 가장 적합한 약종이라고 판단되었다.

Mosquitoes are transmit many dangerous disease such as malaria, yellow fever and dengue fever. So far, chemical insecticides such as DEET have been mainly used to control mosquitoes, but there are many side effects. This study used ultrasonic sounds as an alternative to chemical insecticides. We found that Culex pipiens, which are common in Korea, exhibit avoidance behavior in a specific ultrasonic frequency. Through electrophysiological recording, we have inferred that avoidance behavior is caused by different from each other mechanisms depending on the ultrasonic frequency. Using immunohistochemical staining, we analyzed the expression pattern of auditory related genes in the chordotonal organ. Quantitative real time-PCR was used to compare the expression levels of auditory related gene depending on the time of exposure to ultrasonic sounds.

최근 기후변화로 인하여 다양한 산림곤충들이 대면적으로 발생하여 산림수목에 큰 피해를 주고 있다. 그러나 산림해충의 정확한 발생모니터링이 이루어지고 있지 않으며 대부분의 방제 또한 유기합성농약에 의존하고 있다. 페로몬은 해충의 정확한 발생 모니터링뿐 아니라 대량포획 및 교미교란에 의한 친환경 방제방법으로 널리 활용 될 수 있다. 특히 산림은 다양한 생태계로 구성되어 있기 때문에 유기합성농약을 줄이거나 대체 할 수 있는 방제방법이 시급한 실정이다. 본 발표에서는 주요 산림해충인 천공성 해충류, 곰솔의 주요 해충인 솔껍질깍지벌레, 나비목 해충류에 대한 근래 연구동향에 대해 소개하고 미래의 산림해충 화학생태학의 연구 방향에 대한 제언을 하고자 한다.

Shrimps infected with WSSV (White Spot Syndrome Virus) generally exhibit white spots in their inner space of carapaces as an acute clinical sign. In an effort to identify the correlation between this acute clinical sign and the condition, the index factors (RNA/DNA concentration and ratio, trypsin activity) were analyzed. A total 580 farmed Fenneropenaeus chinensis and 130 Lithopenaeus vannamei were collected from western and southern fifteen outdoor ponds in Korea. The status of the white spot pathology was divided into four stages (stage 0, stage I, stage II, and stage III), in accordance with the clinical signs as to the size and area of white spots. A significant decrease in RNA concentration and RNA/DNA ratio for multi-infected fleshy prawn (WSSV and vibrio sp.) occurred during the stage III (the whole carapace is covered with a white spot). In particular, RNA/DNA ratio was significantly lower as 1.47±0.04 than other groups. A similar trend was also found in the single infection (WSSV), but the decrease was less than the multi-infection. In the species comparison, both species were vulnerable to the multi-infection, but L. vannamei was more sensitive than F. chinensis (ANOVA, p<0.05): A significant decrease in RNA concentration and RNA/DNA ratio was first found in stage II for the former species, while it was found in stage III for the latter species. Trypsin activity was also showed a similar tendency with nucleic acid variation. Multi-infected shrimp showed drastically decrease of trypsin activity. According to the results, clinical signs of the white spot under carapace have an only physiological effect on shrimp if they covered entirely with white spots.

This study was carried out to investigate insect community structure from different habitats in Baengnyeong island.We performed day and night collection at two different habitats (mountain, rural area) of Baengnyeong island from Mayto September in 2015. A total of 2,879 individuals of 404 species, 81 families belonging to 10 orders were collectedand identified. A dominant species was Idisia ornata Pascoe (Tenebrionidae) despite a very low percentage (6.04%) ofthe species among the catches. Results of independent t-test showed a significant high (p<0.001) of species richness onmountain. Also, seasonal results of ANOVA (Analysis of variance) were significantly influential with species abundanceand species richness. The result of NMDS analysis showed that the community structure of the insects from the mountainis different with rural area.

상업적으로 유통되는 젤라틴 캡슐 제품은 소비자의 건강(예: 광우병) 및 종교적 신념에 대한 우려를 야기시킬 수 있다. 젤라틴은 대부분 소, 돼지 등에서 유래한 원료 물질을 가공한 것으로서, 가공 후 그 원료 물질을 분석하는 것은 대단히 어렵다. 따라서 정부 규제기관의 표시사 항 준수여부 모니터링 연구가 주기적으로 필요하다. 본 연구에서는 인터넷에 유통되는 건강기능식품(n = 181)을 대상으로 젤라틴 캡슐의 원료 물질을 종 특이 PCR 방법으 로 분석했다. 55개 제품의 경우 표시사항에 젤라틴 캡슐 원료 물질에 대한 정보를 명시하였으나(예: bovine-, fishand plant-derived gelatin), 126개 제품의 경우 사용 원료에 대한 정보 없이 “gelatin”으로 표시하였다. 이 126개 제품의 젤라틴 캡슐 분석 결과 51개 제품은 소 유래의 젤라 틴을, 31개 제품은 돼지 유래의 젤라틴을, 그리고 44개 제 품은 소와 돼지의 원료를 혼합하여 제작한 블렌딩 젤라틴을 사용한 것으로 밝혀졌다. 따라서 소비자의 알 권리, 종교적 신념 및 건강을 보호하기 위해 젤라틴 캡슐에 사용된 원료 물질을 표시사항에 제공하는 것은 매우 중요하다.

Background:After a stroke, the control of the trunk muscle may be severely impaired. Due to the importance of trunk control in complex daily postures, the ability to adopt a correct sitting posture is considered a determinant of the recovery of independent function after a stroke.Objects:The purposes of this study were to compare differences in buttock pressure between the left and right sides of hemiplegic patients and differences in their pelvic tilting angles (sagittal and coronal planes) after sitting training with visual biofeedback (VBF) in real time.Methods:Twenty-two individuals with unilateral strokes (11 left-side and 11 right-side hemiplegic stroke patients) participated in this study. Buttock pressure was measured using a pressure mat, and pelvic angles were measured using a palpation meter.Results:The asymmetry of pressure between the right and left (first and third chamber) sides was significantly decreased after the VBF training. The measurements obtained using the palpation meter revealed a significant decrease in the pelvic angles pre- versus post-intervention.Conclusion:VBF training may be distribute a patient’s buttock pressure equally while in a sitting posture and increase the length of time a stroke patient can maintain a symmetrical sitting posture. It can also improve pelvic control while sitting in a neutral position.

The world’s climate is changing. Aphids are emerging as lookouts of climate change and they are a good model to understand these environmental changes especially through migratory behavior. We monitored of the occurrence of aphid in same place (Pyeongchang) of seed potato field since 1977 in Korea. Dates of the start of spring migration of these aphids’ species were getting earlier. And, the pick of autumn migration were gradually move to winter season. Moreover, annual total numbers of collected aphids were also dramatically increased during 40 years. There is potential threats of distribution expansion of aphid species in Korea where is the hot spot of climate change with average temperature over 1.7 °C increase for 4 decades. Therefore we hypothesized that aphids’ migration pattern could be associated with temperature

Worldwide, increasing numbers insecticide resistant insect is one of the main problem in agriculture not only in the field but also in the storage. The rusty grain beetle, Cryptolestes ferrugineus is one of the cosmopolitan insect that infests a wide range of stored cereals and related commodities. Until quite recently, phosphine (PH3) has been effective in controlling this species in worldwide including Korea. However, strongly resistant populations of RGB have been detected in Australia that could threaten market access of infested commodities. Resistant populations detected in Australia showed extremely high levels of resistance to phosphine, up to 1300 folds higher than that of susceptible strain. So here we tried to identify their phosphine resistance mechanism based on transcriptome analysis using RNaseq in adult stage. Over 10Gb were sequenced in each strains and some of specific P450 were over expressed in resistance strain.

The genus Diadegma is a well known parasitoid group and some are known to have symbiotic virus. A novel IV was discovered from the calyx of D. fenestrale female. This virus was named as D. fenestrale Ichnovirus (DfIV) and its genome contains 62 circular DNA segments with an aggregate size of approx. 240kb. We examined its gene expression patterns depending on the hosts (hymenopteran host, D. fenestrale and lepidopteran host, Plutella xylostella) and stages of P. xylostella based on RNAseq. Among 62 genome segments, only two segments such C15 and D14 were highly expressed in ovary of D. fenestrale. On the other hands, five segments were differently expressed depending on the developmental stages (3 rd and 4 th instar larvae). Additionally we confirmed integration of DfIV genome segments into the chromosome from the Plutella xylostella via genome sequencing of the lepidopteran host.

Increasing numbers of insecticide resistant insect is one of the main problem in current agriculture. To manage the insecticide resistance, IRAC suggest some tips for farmers : First, sequence or alteration of insecticide based on MOA. Second, choosing the effective insecticide. In this point raised following question : How to distinguish the effective or non-effective insecticide. Until now, bioassay is the right answer. However that is time-consuming, labor-intensive and costly process. So we have been steadily advancing molecular based insecticide resistance diagnosis for supporting and/or substitution of classical bioassay. It can summarized following steps : First, identification of insecticide resistance mechanism. Second, marker development. Third, development molecular diagnosis such as PASA, LAMP etc. Forth, application on field collected insects. Here we discuss some case of these study particular several major pests in highland agriculture.

The objective of this study was to identify the main floral scents and their relative contents in the floral organ of Nelumbo nucifera. N. nucifera flower, a traditional Chinese medicinal herb, is rich in volatile compounds. In this study, the volatile components of N. nucifera flowers were investigated by headspace solid-phase microextraction gas chromatography-mass spectrometry for each organ of the flower: petals, sepals, pistils, and stamens. In total, we identified 39 compounds, among which aliphatics were major constituents, representing more than 94% of petals and sepals volatiles, followed by sesquiterpenes representing more than 69% of pistils and stamens volatiles. Pentadecane, 1-pentadecene, 8-hexadecyne, 8-heptadecene, and β-caryophyllene characterize the scent of the N. nucifera flower. We identified 24 volatiles in petals and sepals, 25 volatiles in pistils, and 18 volatiles in stamens. Among the monoterpenes, 3-Isopropylidene-4-methylcyclohexene, isoterpinolene, p-Menth-2-en-7-ol, and methyl 2,6,6- trimethylcyclohex-1-enecarboxylate were analyzed and identified for the first time from the N. nucifera flower. This study demonstrates that N. nucifera flowers differ greatly in volatile composition depending on the floral organ of the plant.