The present study aimed to analyze the metaproteome of the microbial community comprising harmful algal bloom (HAB) in the Daechung reservoir, Korea. HAB samples located at GPS coordinates of 36°29’N latitude and 127°28’E longitude were harvested in October 2013. Microscopic observation of the HAB samples revealed red signals that were presumably caused by the autofluorescence of chlorophyll and phycocyanin in viable cyanobacteria. Metaproteomic analysis was performed by a gelbased shotgun proteomic method. Protein identification was conducted through a two-step analysis including a forward search strategy (FSS) (random search with the National Center for Biotechnology Information (NCBI), Cyanobase, and Phytozome), and a subsequent reverse search strategy (RSS) (additional Cyanobase search with a decoy database). The total number of proteins identified by the two-step analysis (FSS and RSS) was 1.8-fold higher than that by one-step analysis (FSS only). A total of 194 proteins were assigned to 12 cyanobacterial species (99 mol%) and one green algae species (1 mol%). Among the species identified, the toxic microcystin-producing Microcystis aeruginosa NIES-843 (62.3%) species was the most dominant. The largest functional category was proteins belonging to the energy category (39%), followed by metabolism (15%), and translation (12%). This study will be a good reference for monitoring ecological variations at the meta-protein level of aquatic microalgae for understanding HAB.

‘Big Bang’ 품종은 꽃이 크고 생육이 왕성하며 엽소 및 잎 마름병에 강한 백합 신품종을 육성하기 위하여 2010년 충남농 업기술원 태안백합시험장에서 교배한 OT(Oriental hybrid × Trumpet hybrid) 백합의 종간교잡종이다. 모본으로는 노랑색의 OT백합 ‘Biarits’ 품종을, 부본으로는 백색의 오리엔탈백합 ‘O11-149’ 계통을 화주절단수분법에 의해 교배한 후 2010년부터 2013년까지 기내 종자배양 및 증식 육묘하여 2014년 우수 계통(OT14-6)을 1차 선발하였다. 2016년부터 2018년도에 걸쳐 ‘Nymph’ 품종을 대조품종으로 하여 일반재배를 통한 특성 검정을 실시하였으며, 2016년 화훼연구소 백합 육성계통 평가 회에서 우수계통으로 선발되어 ‘Big Bang’로 명명한 후 충청 남도와 농촌진흥청 농작물직무육성 신품종선정위원회 심의를 거쳐 국립종자원에 품종보호출원 하였다. ‘Big Bang’ 품종의 자연개화는 중부지역에서 6월 16일로 ‘Nymph’ 품종과 비슷하였고 초장은 155.3cm이었다. 적색 반점에 복색(백색+적색)의 화색과 사발화형의 꽃모양을 나타내었다. 재배에 있어서는 진딧물과 잎마름병 등의 병해충 방제를 주기적으로 실시하고, 영양분 관리 등 일반적인 재배관리를 통해 안정적인 구근생산 및 고품질 절화가 가능하다.

This study was designed to explore effective techniques to be used in IERW (Integrated English Reading and Writing) classes for college students. The study last for 15 weeks and included 457 students and 11 instructors at E University. The participants were freshmen who were taking a compulsory English reading and writing class, and the instructors were seven native English speakers and four Koreans. To ensure the effectiveness of IERW classes, it is important to identify what teaching activities are being used and which activities students prefer. To address these issues, the instructors were interviewed regarding their teaching activities in IERW classes. From the interview results, a survey was made that asked students which class activities they consider useful. The results show that most students responded positively to the activities that the instructors were using, with a preference of around 70 percent. Notably, pair or group activities were rated as less favorable than other activities, with a score of under 50 percent. Some implications of the findings and suggestions for teaching activities for IERW classes are provided.

충청북도농업기술원에서는 연한 분홍색의 향기가 있고, 꽃수가 많으며, 개화기간을 오래 지속할 수 있는 속간 교배종 난 ‘핑키뷰티’를 육성하였다. 신품종 ‘핑키뷰티’는 2007년 Phal. pulcherrima × Phal. parishii 교배종을 모본으로, Neof. falcata ‘Hwacheon’을 부본으로, 속간교배하였다. 종자 발아, 재배, 선발, 특성검정은 2008년부터 2014년까지 진행되었다. 생육이 좋고 꽃의 색, 모양이 우수한 계통을 선발 한후, 2015년부터 2017년까지 3년에 걸쳐 국립종자원 신품종 심사기준으로 특성검정 결과, 우수성이 인정되어 2018년 최종 선발하여 “핑키뷰티”로 명명하였다. 주요 특성은 다음과 같다. 화색은 연분홍색으로 Red-purple Group 68D(RHS칼라차트)이며, 향기가 있고 화경색은 암록색이다. 식물체 크기는 12.7cm로 대조품종과 큰 차이를 보이지 않았다. 잎의 폭은 1.6cm로 대조품종인 P. pulcherrima에 비해 좁은 편이다. 화경장은 25.8 cm이며, 소화수는 16.3개로, 전반적으로 화분의 볼륨감이 우수한 편이다. 꽃의 크기는 2.5cm 정도이며, 꽃의 순판 뒤쪽에 풍란과 같은 짧은 거가 있는 것이 특징이다. 개화기는 5월 하순이며, 개화기간은 34일 정도이다.

We studied the infection rate of and various metacercariocidal approaches to controlling Gymnophalloides seoi for prevention of human infection in cultured and natural oysters in Korea. The selected survey areas were Aphae-do (Shinan-gun, Jeollanam-do), which is an endemic area for G. seoi, and Tongyeong (Geonsangnam-do), which is the main production area of oysters in Korea. In the Tongyeong area, the metacercariae of G. seoi were not detected in cultured oysters (0/201) or wild oysters (0/134). Seventy-two G. seoi metacercariae were observed in 33 of 265 natural oysters collected from Aphae-do; however, metacercariae were not detected in the cultured oysters (0/1101) purchased from the Daejeon Fish Market. To investigate the viability of G. seoi metacercariae, various metacercariocidal treatments were used with 3.5% saline and oyster juice used as positive controls. The metacercariae survived for 75.4 h in 3.5% saline and 112.6 h in oyster juice. After the metacercariocidal treatment, G. seoi metacercariae were survived for 13.29 min in tap water, < 20 sec in 4.3% vinegar, no effect in a rinse of the whole oyster body in 70°C water for 1 sec, but 1 sec in a rinse of the whole oyster body in 90°C water for 1 sec. The greatest metacercariocidal effect on G. seoi was from rinsing oysters in 90°C water followed by those from treatment with 20% ethyl alcohol, 4.3% vinegar, and tap water. However, we suggest that the most actual prevention to G. seoi human infection is rinsing the oysters with tap water for at least 30 min.