This study was conducted to examine the effect of new inoculants on in vitro digestibility and fermentation characteristics of high moisture rye silage. Rye was harvested at heading stage and divided into 5 treatments, following: No additives(CON); L. plantarum R48-27(NI1); L. buchneri R4-26(NI2); mixture of NI1 and NI2 at 1:1 ratio(MIX); and L. buchneri(LB). The rye forage was ensiled into 10 L bucket silo for 100 days. In vitro digestibility of dry matter and neutral detergent fiber were highest(p<0.05) in NI2 silage. The pH in NI2 and LB silages were lower(p<0.05) than CON silage. Lactate concentration was highest(p<0.05) in NI1 silage. While concentrations of acetate and propionate were highest(p<0.05) in MIX silage. Lactates : acetate ratio was highest(p<0.05) in NI1 silage, but lowest in LB silage. Butyrate concentrations of NI2 and LB silages were lower(p<0.05) than that in CON and NI1 silages. Lactic acid bacteria (LAB) count in all inoculated silages was higher(p<0.05) than that in CON silage, while yeast count in LB silage was lower than in CON, NI1, and MIX silages. In conclusion, application of NI2 inoculant could improve potentially fermentation quality and digestibility of high moisture rye silage.

In order to develop the D. adamsi breeding technique that is highly likely to be used as an emotional pet insect, the results of the D. adamsi was collected in the large exhibition Jang Tae-san in Daejeon to the indoor breeding began to spawn after 10 days after the training, the egg laying period was 33.6 days, the average number of spawn per female was 27.7, and the period was 11.8 days. D. adamsi larvae bred with food of oak-boiled molted two times, the duration of 1st larvae development was 11.8 days, 2nd larvae 14.5 days, and the third larvae was 29.4, and the larvae were used to build houses using Masato(soil) and in the pupae, and then in April of the following year. D. adamsi has a characteristic to build a house with fallen leaves and spawn one or two eggs in the fallen leaves, and the results of the spawn mat test using fallen leaves, leaf mold and a sawdust, etc., and the number of spawn was high in the Masato was spread about 3 ~ 5cm, and a fallen leaves on a 10 cm or so, and the growth and survival rate of the larvae were also high. Larvae breeding density was 2 ~ 3 ℓ in the container size to raise the object was normal growth, the higher the density mortality was high and the growth was sluggish. Larvae feeding conditions were normally developed in dry fallen leaves and fermented fallen leaves, compared to the sterile fallen leaves, oak sawdust, sterile fallen leaves and oak sawdust was abnormal, the mortality rate was higher than 50%.

후각 신경 시스템은 일반 생활 환경에서 많은 다양한 화학 물질을 인식하고 구별한다. 곤충에서는 다양한 화학 물질을 기호적 또는 회피적인 특이성을 부여하고 이를 구분해 낼 수 있는 고도로 발달된 후각신경 수용체들로 구성된 냄새 맡는 (odorant-gated) 이온 채널 군을 진화시켰다. 최근에 후각 수용체와 단백질을 포함한 olfaction 관련한 진딧물 게놈 밝혀졌고, 초파리에서 다양하게 분화되어 있는 후각 수용체들이 보고되고 있다. 후각 신경 수용체의 유전체는 매우 높은 보전적인 염기 서열을 가지고 있으며, 체계적인 신호 전달 시스템을 갖추고 있다. 대표적 수용체인 odorant-gated ion channels comprised of a highly conserved co-receptor (Orco)는 중심 구멍 주위에 대칭 적으로 배열된 4 개의 서브 유닛을 갖는 homotetramer 채널 구조를 가지고 있다. 이는 인체 내에 존재하는 7-transmembrane receptor와 매우 유사한 구조를 형성하고 있고, 신경전달물질의 수용체와 매우 유사한 구조적 형태 및 gating mechanism을 가지고 있다. 본 연구에서는 초파리에서 분리한 후각 신경 수용체 하위 유형인 OR65 유전자 분리하여 세포 발현 시켜 Xenopus oocyte를 이용하여 Whole cell voltage clamp recording을 실시하였다. 본 수용체의 성공적인 발현 이후 유해 해충 유인제 개발 회사인 마이크로자임의 미생물 배양 추출물을 이용해서 후각 신경 수용체 활성 조절 여부를 연구하였다. 미생물 배양 추출물을 10,000배 희석한 recording media에서 수용체의 활성을 확인하였고, 이를 농도 별로 처리하여 농도 의존성 수용체 활성 작용을 확인하였다. 따라서 곤충의 후각 신경 수용체 활성 조절 시스템을 이용하여 유인물질 또는 기피 물질을 발굴할 수 있으며, 본 연구를 통해서 MZ01은 곤충 수용체 OR65를 활성 시킴으로써 유인 현상을 나타내며, 본 연구를 통해서 현장에서 검증된 미생물 배양 추출물의 성능을 과학적 분석으로 결과를 제시하였다.

소나무재선충병의 매개충인 북방수염하늘소의 체내 선충보유량에 따른 비행능력의 차이를 구명하기 위하여 춘천시 지내리의 잣나무림에서 어리소나무재선충(Bursaphelenchus mucronatus)에 감염이 확인된 북방수염하늘소 암·수 각 20마리를 채집, 실내사육 후 우화-섭식 1 주일이 지난 시점부터 1주 간격으로 Flight mill을 이용하여 비행실험을 진행하였다. 동시에 사육통 바닥에 여과지와 섭식한 잣나무 신초를 3~4일 마다 교체 후 Baermman funnel법으로 소나무 재선충을 분리하여 그 수를 조사, 비행능력과 선충보유량의 관계를 조사하였다. 그 결과 약 50,000마리 이상 선충을 체내에 보유시 비행능력이 급감하는 것을 확인 할 수 있었으나, 전체적으로 선충의 체내 보유량에 따른 비행능력의 차이는 상관관계가 없는 것으로 확인 되었다.

최근 지구온난화로 인한 기후변화는 육상 및 해양 생태계에 다양한 영향을 미치고 있다. 농업생태계 역시 이들 생태계에 의존하고 있는 생물 및 인간에게 생물학적, 경제학적, 사회학적으로 다양한 영향을 주고 있다. 기후변화를 쉽게 감지할 수 있는 지표종은 기후변화에 비교적 민감하게 반응을 나타내기 때문에 농업생태계와 같은 경제 사회적 영향을 많이 받는 곳에서 다양하게 활용될 수 있다. 2017년 농업과학원에서는 농업생태계에서 기후변화에 따른 영향을 잘 나타낼 수 있는 식물과 무척처동물 30종을 지표종으로 선정하였다. 30종 중 나비목에 속하는 종으로는 배추흰나비(Pieris rapae), 남방노랑나비(Eurema mandarina), 노랑나비(Colias erate), 호랑나비(Papilio xuthus) 4종이다. 이 연구는 나비 지표종 중 농업생태계에서 가장 풍부하고 확인이 쉬운 배추흰나비를 대상으로 전남, 충북, 경기, 강원지역에서 4월부터 월 1-2회 모니터링을 실시하여 생물계절의 차이를 알아보았다. 조사는 각 지역에서 논과 밭, 산림 등을 포함하는 경로를 선정하여 30분간 이동하면서 좌우 5m내외 출현하는 나비를 조사하는 선 조사법을 실시하였다. 4월 이후부터 조사한 결과 전남에서는 4월초인 14째 주에 이미 많은 수가 관찰된 반면 충북, 경기 등지에선 15째 주 이후 관찰되기 시작하였다. 강원도에서는 6월 중순인 24째부터 관찰되어 위도별 출현 양상의 차이를 나타내었다. 9월말까지 관찰된 생활사 수는 전남에서는 5회, 경기도에서는 4-5회, 충북에서는 4회, 강원에서는 2회로 지역별 차이를 나타내었다. 이러한 결과는 농업생태계에서 흔히 볼 수 있는 배추흰나비가 위도별로 출현시기와 출현횟수를 달리하면서 나타나는 것을 통해 기후변화를 나타낼 수 있는 지표종으로 적절하다고 여겨지며 앞으로도 전국적으로 지속적인 모니터링을 통해 정밀한 출현양상과 미래 분포 변화 모델링 작업에 효과적으로 이용될 수 있는 기초자료를 제공할 수 있을 것으로 여겨진다.

미국선녀벌레(Metcalfa pruinosa)는 외래 침입해충으로 조사가 시작된 2009년 이후 발생지역과 발생면적이 계속 증가하여 2018년에는 123개 시군구의 21,154ha에서 발생이 보고되고 있다. 미국선녀벌레는 농경지와 그 주변 산림지에서 활동하기 때문에 정부와 지자체간 협업을 통해 농림지 동시방제를 5월하순경(부화약충 대상)과 7월 중순경(성충 대상) 2회 실시하고 있다. 농촌진흥청에서는 미국선녀벌레의 기생천적인 선녀벌레집게벌(Neodryinus typhlocybae)의 안정적인 도입과 미국선녀벌레에 대한 생물적 방제원으로서 국내 정착을 위해 이탈리아 파도바대학과 국제협력사업을 2017년부터 수행하고 있다. 선녀벌레집게벌은 완주에서 월동이 가능하였으며 6월 초에 50%이상 우화를 하였다. 2018년도에 온실(완주)과 야외(수원, 태안)에서 증식하여 1,000여 개체 이상을 확보하였으며 도입천적의 안정적인 국내 정착과 확산 및 활용 방안에 대해 논의하고자 한다.

This investigates the microstructure and mechanical properties of Al hybrid material prepared by electromagnetic duo-casting to determine the effect of heat treatment. The hybrid material is composed of an Al-Mg-Si alloy, pure Al and the interface between the Al-Mg-Si alloy and pure Al. It is heat-treated at 373, 573 and 773K for 1h and T6 treated (solution treatment at 773K for 1h and aging at 433K for 5h). As the temperature increases, the grain size of the Al-Mg-Si alloy in the hybrid material increases. The grain size of the T6 treated Al-Mg-Si alloy is similar to that of one heat-treated at 773K for 1h. The interface region where the micro-hardness becomes large from the pure Al to the Al-Mg-Si alloy widens with an increasing heat temperature. The hybrid material with a macro-interface parallel to the tensile direction experiences increased tensile strength, 0.2% proof stress and the decreased elongation after T6 heat treatment. On the other hand, in the vertical direction to the tensile direction, there is no great difference with heat treatment. The bending strength of the hybrid material with a long macro-interface to the bending direction is higher than that with a short macro-interface, which is improved by heat treatment. The hybrid material with a long macro-interface to the bending direction is fractured by cracking through the eutectic structure in the Al-Mg-Si alloy. However, in the hybrid material with a short macro-interface, the bending deformation is observed only in the limited pure Al.

An earthquake of ML 5.8 hit the Gyeongju area on September 12, 2016. A sequence of foreshock-mainshockaftershock of 588 events with equal to or greater than magnitude 1.5 occurred for six months in this area. Around ninetynine percentage (98.8%) of the total energy was released intensively within a day, and about 80% of the total events took place within a month after the Gyeongju earthquake. The epicentral distribution of aftershocks of major events (ML 5.1, 5.8, 4.5, and 3.5) were elongated in the direction of N30 o E. They correlate well with the focal mechanism solution. These facts support the inference that the Gyeongju earthquakes occurred on a sub-parallel subsidiary fault of the Yangsan fault zone or on the linking damage zones between Deokcheon and Yangsan fault. During the last six years before the Gyeongju earthquake, there were few events within 10-km radius from the epicenter. This seismic gap area was filled with a sequence of the Gyeongju earthquakes. The b value for aftershock of the Gyeongju earthquakes is 1.09.

The objective of this study was to investigate the dynamic neural mobilization program on the changes in muscle activity and nerve conduction velocity (NVC) in stroke patients. The participants were sampled and randomly divided into experimental group I (n=12) who underwent arm neural mobilization and experimental group II (n=13) who underwent arm dynamic neural mobilization. As the pretest, peripheral NVC of the radial, median, and ulnar nerves were measured using the Viking Quest; the biceps brachii, brachioradialis, flexor carpi radialis, and extensor carpi radialis activities were measured with sEMG. Each intervention program consisted of 10 trials per set and three sets per session. The intervention programs were performed once daily for four weeks (four days/week). Posttest measurements were taken equally as the pretest measurements. Significant differences in peripheral NVC in all sections of the radial and median nerves and wristbelow elbow and below elbow-above elbow areas of the ulnar nerve, as well as in muscle activity of all muscles except the biceps brachii. These findings indicate that dynamic neural mobilization was effective in increasing peripheral NVC and altering the muscle activity.

This study was carried out to estimate the effect of selected inoculants on chemical compositions and fermentation characteristics of rye silage. Rye was harvested at dough stage and divided into 5 treatments, following: No additives (CON); L. plantarum R48-27 (LP27); L. buchneri R4-26 (LB26); Mixture of LP27 and LB26 at 1:1 ratio (MIX); and L. buchneri (LB). The rye forage was ensiled into 10 L bucket silo for 100 days. The contents of NDF and ADF were lowest (P<0.05) in LB26. The pH in LB26, MIX, and LB were lower (P<0.05) than CON and LP27. Lactate content in LB was higher (P<0.05) than the others, while acetate content in LB26 and LB were higher (P<0.05) than that in CON and LP27. Lactate to acetate ratio was highest (P<0.05) in LB, but lowest in LB26. Lactic acid bacteria (LAB) count in LB was higher (P<0.05) than that in CON, while yeast count in CON was lower than in all silages applied inoculants. In conclusion, silages inoculated with LB26 could improve potentially the aerobic stability caused by increases of acetate and propionate concentrations.

The investigation of the embryonic development of the cerebellum has a long history. The postnatal normal development of the cerebellum in rodents and other animals became a popular topic for morphological investigations nearly a century ago. However, surprisingly, only a few studies are available regarding the prenatal normal development of the rodent cerebellum, especially in guinea pigs. Cell proliferation is essential for the development of the nervous system. The assessment of cell proliferation can be achieved by using various methods. In this study, we investigated the cell proliferation of the cerebellar cortex in guinea pigs at different stages of pregnancy and in postnatal life. Fetuses were obtained by cesarean section at 50 or 60 days of gestation (dg). Immunohistochemistry was performed with proliferating cell nuclear antigen (PCNA) antibody in the cerebellum. Strong PCNA immunoreactivity was observed in the external granular layer (EGL), which is a neurogenic zone in the cerebellum. The proportion of PCNA-IR cells was greater at 1 week than at 60 dg in lobule I, but not lobule VIII. After 50 dg, the width of the EGL continued to decline until 1 week, due to the maturation of the EGL cells. These results demonstrate the pattern of PCNA immunoreactivity in the developing cerebellum of guinea pigs. This serves as a guideline to study abnormal cerebellum development.

Recently, there is an increasing the pavement distresses such as rutting with an increase in heavy vehicles on the road in Mongolia. Rutting is the longitudinal depression in the wheel path in asphalt pavements and it causes a hydroplaning and severe safety concern for users. This study aims to develop paving material that can prevent rutting on the road pavement by improving the durability of the asphalt mixture in Mongolia. Therefore, this study was carried out using the technique of reinforcing the material by adding fibers to conventional asphalt mixture. Fibers have been used to reinforce various materials for many decades in various parts of the world. It is generally understood that asphalt is strong in compression and weak in tension. Adding fibers with high tensile strength can help increase the strength of a mixture[1]. A mixture of glass fibers was used in this study to evaluate the performance characteristics. In coordination with the City of Ulaanbaatar, The test section selected in this study was Peace Avenue in Ulaanbaatar. The test section was a bus lane with severe rutting by heavy vehicles. The designated road test section performed cutting and overlay using five asphalt mixtures: Glass Fiber-reinforced Asphalt, Hot Mix Asphalt(10mm, 19mm), Polymer Modify Asphalt(2 types). The performance survey was conducted after the summer. As a result, No noticeable cracks were observed in glass-reinforced mixture section and the rut-depth of the glass-reinforced mixture is lower than other mixtures[2].

To be better fit for highways, pavement systems are required to provide comfortable and safe driving and be structurally durable. Composite pavements can be an effective option as they are more durable by placing a high functional asphalt overlay on a rigid concrete base layer. In order to apply a composite pavement system to the field, it is necessary not only to develop technologies that prevent reflecting crack and deterioration of the base layer, but also to improve bonding performance of materials and ensure structural performance as a pavement system against traffic loading. In advanced countries like Japan, USA and Europe, high-functional composite pavement systems are being put into practice across new highway networks. In this study, we evaluated structural performance (rutting, reflecting crack, and deflection) by applying traffic loads of actual highways through an accelerated pavement tester (APT) of a composite pavement section made up of a quiet porous surface laid over a water-proofing layer, a continuously reinforced concrete base, and a lean concrete sub-base layer, which was developed with new pavement methods used for each layer prior to field application. The APT specimen was constructed with paving materials and equipment actually used on site in the same dimensions (W3.5m*L14m*H2m) as actual highway sections in Korea, and 3-axle double-wheel heavy load (45ton) cart type KALES(Korean Accelerated Loading and Environmental Simulator) traveling on the specimen in both directions was used to simulate traffic loading. After applying around 8,574,000 ESALs of traffic loads, no reflecting crack occurred on the asphalt surface of the composite pavement, without surface distress except for rutting. In order to examine what causes rutting of pavements, we surveyed thickness of pavements by layer and measured asphalt density.

Recently, Cambodia has been investing a lot of money in the construction and maintenance of roads, which are social infrastructures. However, damage to the existing pavement is accelerating due to the old age of the road pavement, poor drainage facilities and increase in heavy traffic. To solve this problem, a fundamental solution such as a high-performance asphalt mixture is required to extend the life of road pavement. In this study, a high performance glass fiber reinforced asphalt mixture developed in Korea was applied to Cambodia. Prior to field application, Marshall stability tests were performed on glass fiber reinforced asphalt mixtures, SBS modified asphalt mixtures and asphalt mixtures commonly used in Cambodia. The Marshall stability test showed that the glass fiber reinforced asphalt mixture and the SBS modified asphalt mixture had the same strength (about 1.3 times higher strength than the usual asphalt mixture). In addition, the test construction was carried out on the National Highway 2 of Cambodia for the evaluation of the performance of the three mixtures. In the future, the long-term performance evaluation of each mixture will be conducted through follow-up survey of the test construction area.

Roller Compacted Concrete Pavement (RCCP) is placed by roller compaction of a mixture of less cement and unit water content and more aggregates and provides excellent early strength development with the help of interlocking of aggregates and hydration. The unit cement content of RCC pavements accounts for 85% of conventional pavements, with low drying shrinkage. As low drying shrinkage leads to smaller crack widths than ordinary concrete, RCC pavements can help elevate reflecting crack resistance if applied to a base layer of a composite pavement system. In a composite pavement with an asphalt surface laid over a concrete base, pavement temperature change is important in predicting pavement performance. As movement of the lower concrete layer is determined by temperature depending on pavement depth, temperature data of the pavement structure serves as an important parameter to prevent and control reflecting crack. Among the causes of reflecting crack, horizontal behavior of the lower concrete layer and curling-caused vertical behavior of joints/cracks are considered closely related to temperature change characteristics of the lower concrete course (Baek, 2010). Previous studies at home and abroad about reflecting crack have focused on pavement behavior depending on daily and yearly in-service temperature changes of a composite pavement (Manuel, 2005). Until now, however, studies have not been conducted on initial temperature characteristics of concrete in composite pavements where asphalt surface is placed over an RCC base. Annual temperature changes of in-service concrete pavements go up to 60 ℃, and those of asphalt overlays become around the twice at 110 ℃. This study evaluated initial crack behavior of composite pavement by investigating pavement temperature by depth of an RCC base and analyzing joint movement depending on change to temperatures of continuously jointed pavements. Findings from the study suggest that in composite pavements and asphalt overlays, time of laying asphalt has an important impact on crack behavior and reflecting crack.

Roller Compacted Concrete Pavement (RCCP) is a pavement placed and compacted using an asphalt paver and a compaction roller by applying a small amount of concrete mixture and shows excellent structural performance as a result of hydration reaction of cement and interlocking of aggregates by roller compaction. It also provides economic advantages over conventional concrete pavements by reducing unit cement content and construction period, simplifying construction process, and decreasing traffic closure time (Wayne, 2006). However, given that it tends to show lower IRI levels than common concrete pavements since its low unit water content and binder weight ratios make uniform quality control difficult and roller compaction after paving makes the surface irregular and rough, with rough profile at the bottom of the pavement being reflected on the surface, RCCP is used mainly in port and industrial roads for low speed (60km/h or less) traffic (Dale Harringtion, 2010; Gregory, 2009). In order to apply RCCP to high-speed roadways, diamond grinding (DG) or asphalt overlay that is highly effective in improving roughness is needed (Fares Abdo, 2014; Gregory, 2009). Applying DG over RCCP leads to excellent skid resistance and noise reduction effects as a great percentage of aggregates makes the pavement surface rough, enhancing durability of concrete and the life of DG functionality. In addition, RCCP can be used as a high performance base layer of composite pavements, as it can reduce reflecting cracking at joints and cracked sections thanks to early strength development and low drying shrinkage of concrete. In this study, we assessed longitudinal roughness improvement effects by roughness-affecting factor by applying DG methods and asphalt overlays to three RCCP sites with a variety of sub-structural conditions and analyzed the effects on roughness of existing RCC pavements depending on surfacing method (DG, APOverlay).