Pine wilt disease (PWD) induced by the pinewood nematode (PWN), Bursaphelenchus xylophilus, is a great threat to pine forests in Europe and East Asia. Identification of interrelation between Monochamus spp., the major vectors of PWD and PWN is the key factor for effective control of PWD because PWN is trasmitted to healthy pine trees only by Monochamus vectors. In this study, direct injection system is designed to apply PWN artificially to M. alternatus just before emergence to adults. After injection of PWN (300~500 nematodes in 10 μl of 1X PBS) using adapted glass pipets, the survival rate of M. alternatus and detection rate of live PWN were 80.0% and 68.8% at 168 h respectively. As a result, we demonstrated the artificial injection methods of nematode to Monochamus vectors for further study of coeffect between vectors and specific stage of PWN including 3rd stage dispersal juvenile.

Red meats are important animal foods because of their nutritional aspects, but the over-consumption of red meat produces reactive oxygen species (ROS) caused by heme iron and induces colorectal cancer. The effect of orally administered hemin and calcium provided in drinking water for 6 weeks on colon carcinogenesis was observed in male ICR mice. After the mice were acclimated for 1 week, they received three subcutaneous azoxymethane (AOM, 10 mg/kg b.w.) injections weekly and were provided with 2% dextran sodium sulfate (DSS) via drinking water for the next week. The mice were divided into three groups: the control, hemin, and hemin + calcium groups. The orally administered daily dose of hemin was 2 g/kg b.w., and 0.05% calcium was provided daily via drinking water. Colonic mucosa samples were stained with methylene blue, and then, the numbers of aberrant crypt (AC) and aberrant crypt foci (ACF) were counted. Lipid peroxidation in feces was estimated by thiobarbituric acid-reactive substances (TBARS) assay. The total numbers of AC and ACF per colon in the hemin group were significantly higher than those in the control group. Calcium treatment significantly decreased the numbers of ACF and AC in the colon of mice. The TBARS value in the feces of the hemin + calcium group was significantly lower than that in the feces of the hemin group. These results showed that hemin enhances the formation of pre-neoplastic lesions in the colon of mice and that calcium decreases the risk of colon carcinogenesis.

The aim of the study was to investigate the effects of incentive spirometry and Ujjayi breathing technique on the pulmonary function of smokers. Subjects were individuals who had a smoking habit of at least a year. Subjects were randomly divided into 3 groups: the incentive spirometry group (n=8), Ujjayi breathing technique (n=9), and a group applying both incentive spirometry as well as Ujjayi breathing technique (n=8). Each intervention was performed twice a day, 5 times a week, for a total of 8 weeks whereupon the change in pulmonary function was evaluated. A spirometer was used to measure FVC, FEV1, and FEV1/FVC. The survey used for this study included the Fagerström Test of Nicotine Dependence (FTND) and the Shortness of Breath Questionnaire (SOBQ). Study results for the comparison within groups showed that in the group that performed both the incentive spirometry and Ujjayi breathing technique, FEV1 improved with statistical significance (p<.05). Furthermore, within this comparison the FEV1/FVC improved with statistical significance. Comparison amongst the groups showed no statistically significant differences in all areas. Following, to effectively increase pulmonary function in young adult smokers, both incentive spirometry and Ujjayi breathing technique should be employed together.

Colorectal cancer (CRC) is the third most prevalent cancer in the world, and heme iron is known to promote the CRC in an animal model. This study was conducted to investigate the effects of ascorbic acid in the presence of hemin on the formation of pre-neoplastic lesions induced by azoxymethane (AOM)/disodium sulfate (DSS) in mice. After acclimation for 1 week, five-week old mice received three s.c. injections (0-2 weeks of the experiment) of AOM [10 mg/kg body weight (BW)] weekly and were treated with 2% DSS in drinking water for the next week to induce aberrant crypt foci (ACF). All animals were fed the AIN-76A purified rodent diet for experimental period of 6 weeks. Experimental groups were then divided into three groups: carboxymethylcellulose (CMC) alone (control), CMC + Hemin, CMC + Hemin + ascorbic acid (AA). The CMC was used as a solvent for hemin. The daily doses were 534 mg/kg BW hemin and 246 mg/kg BW ascorbic acid administered orally. After the colonic mucosa were stained with methylene blue, aberrant crypt foci (ACF), aberrant crypt (AC) and polyps were counted. Lipid peroxidation in liver was evaluated by the thiobarbituric acid-reactive substances (TBARS) assay. The numbers of ACF, AC and large ACF (≥4 AC/ACF) per colon increased in the hemin group compared to the control group, while they decreased significantly in the hemin + ascorbic acid group compared to the control group or hemin group (p<0.01). The number of polyps/colon in the hemin + AA group was significantly decreased compared to the hemin group (p<0.05). In the liver, the TBARS value of the hemin group was significantly higher than that of the control group (p<0.01). Additionally, the TBARS value of the hemin + AA group decreased slightly compared to that of the hemin group. Taken together, these results suggest that hemin can promote colon carcinogenesis in a mouse model and that ascorbic acid has a protective effect against hemin-promoted colon carcinogenesis.

Excessive iron can promote the production of free radicals, thereby leading to harmful effects on cancer and aging. Ascorbic acid is not only an antioxidant but also a co-factor of iron absorption. The effect of iron-overload with ascorbic acid on experimental colon carcinogenesis was investigated in male ICR mice. Animals were treated weekly with azoxymethane (AOM, 10 mg/kg b.w.) at 0, 1, and 2 week and then drunk 2% dextran sodium sulfate (DSS)-containing water for the next 1 week. There were four experimental groups: carboxymethylcellulose (CMC) alone (control), CMC + ascorbic acid (AA), CMC + Fe, CMC + Fe + AA. The animals fed on AIN-76A purified rodent diet for six weeks. AA or Fe2O3 at the dose of 450 mg/kg b.w. were daily and orally treated for 6 weeks. The colonic mucosa was stained with methylene blue and then aberrant crypt foci (ACF) and polyps were counted. Thiobarbituric acid-reactive substances (TBARS) in serum and liver were determined. Iron concentration in liver was measured by inductively coupled plasma spectrophotometer. Fe-overload with AA strongly increased liver iron contents compared to control or Fe group (p<0.05). There were no significant differences in the number of ACF or polyps among all groups, although ironoverloaded groups had slightly higher numbers compared with the control or AA group. TBARS values in the liver were increased in the iron-overloaded groups compared to control and AA only group (p<0.05), but serum TBARS values were not changed. These results indicate that the excessive iron treatment did not affect the experimental colon carcinogenesis regardless of presence of AA in mice.

The electronic and optical characteristics of molybdenum disulphide (MoS2) film significantly vary with its thickness, and thus a rapid and accurate estimation of the number of MoS2 layers is critical in practical applications as well as in basic researches. Various existing methods are currently available for the thickness measurement, but each has drawbacks. Transmission electron microscopy allows actual counting of the MoS2 layers, but is very complicated and requires destructive processing of the sample to the point where it will no longer be useable after characterization. Atomic force microscopy, particularly when operated in the tapping mode, is likewise time-consuming and suffers from certain anomalies caused by an improperly chosen set point, that is, free amplitude in air for the cantilever. Raman spectroscopy is a quick characterization method for identifying one to a few layers, but the laser irradiation causes structural degradation of the MoS2. Optical microscopy works only when MoS2 is on a silicon substrate covered with SiO2 of 100~300 nm thickness. The last two optical methods are commonly limited in resolution to the micrometer range due to the diffraction limits of light. We report here a method of measuring the distribution of the number of MoS2 layers using a low voltage field emission electron microscope with acceleration voltages no greater than 1 kV. We found a linear relationship between the FESEM contrast and the number of MoS2 layers. This method can be used to characterize MoS2 samples at nanometer-level spatial resolution, which is below the limits of other methods.

The population of managed honeybees has been dramatically declining the recent past in worldwide. N. ceranae causessignificant detriment to honey production and results in economic losses critically. In our knowledge, Fumagillin is theonly antibiotic approved for control of nosemosis in honeybees. In this study, to select isolate with anti-Nosema activityagainst N. ceranae. Entomopathogenic fungi cultural filtrates were screened using in vitro polar tube germination assay.These fungi cultural filtrates were used to evaluate the safety of honeybees and their inhibition of nosemosis. As a result,P. marquandii 364 and Pochonia sp. 60 showed inhibitory activity on the growth of N. ceranae in honeybees and didnot significantly affect the survival rate of honeybees. These may be employed as antibiotic agents and a good featureto be used in the development of new biocontrol agents of nosemosis.

The baculovirus expression vector system (BEVS) is an effective and widely used system for the production of recombinantproteins in insect cells or larvae. However, the expression efficiency of recombinant proteins using the polyhedrin promotercould not acquire the protein yields observed for native polyhedrin. In this study, we tried to develop hyper expressionvector by the optimal combination of previously reported various enhancer factors. The selected enhancer factors for optimalexpression consists homologous region5 (hr5), VP39 promoter and burst sequences. Seven recombinant viruses were madeto compare EGFP expression level. Each recombinant viruses showed different expression levels respectively, and themost of expression level was observed with higher than those of the previous vectors. This study suggests a new optionfor hyper expression of useful recombinant protein using the BEVS.

The two-spotted spider mite, Tetranychus urticae, and gray mold disease caused by Botrytis cinerea, are importantpest and plant disease. To control these, many people have been relied on chemical methods for a long time. However,their continuous use has resulted in resistance of pest and disease. To surmount or avoid these problems, we evaluatedantifungal activity of the selected fungi with high virulence to mite to explore the potential for the dual control of notonly T. urticae but also B. cinerea. To maximize the use of spores and cultural filtrates, the virulence to mite was evaluatedusing culture products, and effective culture condition was investigated against blastospore production and antifungal activity.Consequently, the 2 fungal isolate selected in this study were confirmed to have diverse potential and would be usedeffectively for dual control agents against the two-spotted spider mite and plant diseases

It is necessary to understand of temporal and spatial dynamics by establishing a periodical monitoring system for theproper management in small brown planthopper (SBPH). A dataset is including the number of SBPHs by location, collectionmethod [aerial collection net (AeCN) or light trap (LT)] and period (May~Aug.) for five years (2011~2015), and missingvalues were imputed using multiple imputation methods. Of the 15,848 individuals collected, approximately 47% and 52.9%were collected using the AeCN and LT methods, respectively. A high incidence of migratory SBPHs was observed duringJulian days 144-166 using the AeCN method. Generally, the migratory SBPHs from China composed 39.4% of the totalpopulations of SBPHs. These results would provide valuable information to predict the incidence period of migratory SBPHsand establish a proactive management system against SBPH.

The symptoms of pine wilt disease caused by the pine wood nematode (PWN, Bursaphelenchus xylophilus), whichis recognized as a major forest pest. The PWN is transmitted by Monochamus saltuarius adults to healthy Korean whitepine (Pinus koraiensis). Because flight capacity of vector insects is important to protect pine forests, we tested flightdistance of M. saltuarius adults using flight mills in relation to biological characters (sex, age, and body weight). In addition,flight distance of M. saltuarius adults was compared between mated and unmated beetles to test effect of sexually matureon flight capacity. Although unmated adult beetles were more rapidly die compared with mated adults, total cumulatedflight distance during entire lifespan was differed according to whether mating or not, i.e., unmated adult beetles moretraversed than mated adults. Proportion of intermittent flights in mated females was significantly lower than others.

Walnuts are economically important for forestry workers in recent, but there are few available data for insect pestsin walnut orchards. In 2017, walnut fruits and leaves were damaged by Conogethes punctiferalis and Acrocercops transecta,respectively. In late summer, damage rates by C. punctiferalis in Buyeo, Gimcheon, and Hwaseong were 22.1%, 20.5%,and 11.7%, respectively. According to A. transecta, damage rates in seedlings (58.5% in average) were higher than inolder trees (11.2% in average) irrespective of study locations.

Wildfire is one of important disturbances in forests, which may effect on insects distribution. In Sangju, about 13 haof pine forests was burned in March, 2017. We investigated the response of insects to fire severity in burned pine forests,Sangju. Study area was classified by fire severity as 5 classes, such as unburned, light, light-moderate, moderate-severe,and severe. We placed 4 multi-funnel traps in each fire severity class. A total of 729 insects belonging to 42 specieswere collected during May 24 to October 12, 2017. Spondylis buprestoides (447 individuals), Sipalinus gigas (50 individuals),and Acanthocinus carinulatus (43 individuals) were dominant species accounted for 74.1% of total. According to fire severity,abundance and species richness were increased with fire severity, especially in high-medium area. Interestingly, two Monochamusspecies, vector insects of pine wood nematodes, were more caught in more severed area than in other classes.

Viral particles of Porcine epidemic diarrhea virus (PEDV) consist of a four structural proteins. Among them Spikeprotein mediated responsible for receptor binding and membrane fusion during viral infection and therefore the main targetof neutralizing antibodies. Virus-like particles (VLPs) are consisted of one or more viral structural proteins, and theirmorphologies closely resemble those of the native virus. VLPs have no virulence and can elicit robust immune responsesas compared with inactivated or live-attenuated virus vaccines. Thus, in this study, we tried two methods for VLP constructionin Bombyx mori, one is traditional method and the other is chimeric VLP method using the influenza matrix protein.Both methods could produce successfully PEDV VLPs.

The purpose of this study is to improve practical skill evaluation method of mushroom - trained certified technician's practical skill evaluation which is one of national qualification tests based on national incompetency standards. One of the current National Competency Standards (NCS), mushroom - trained certified technician uses NCS based practical assessment method. In order to improve the current practical evaluation method, we try to improve practical evaluation method based on field customized problem solving ability and improve the practical evaluation method, various evaluation methods should be constructed. On the purpose of identify the diversity and problems of the evaluation method, the experts of the group consultation, the mushroom-related research institute and the related industry collaborated to identify the problems of the actual mushroom - trained certified technician practicum test, This study on the evaluation improvement method was carried out. In this study, the contents of practical test of the current mushroom traits were analyzed and the trends of the latest mushroom industry were widely reflected.

This study is investigated the growth characteristics(number of available stipe, pileus diameter, pileus thickness, stipe length, stipe thickness, object weight, comparison yield ability of 1 cycle) and storage characteristics of ‘Sanjo 701’ (S7) cultivars according to relative humidity. The S7 growth characteristics were investigated by quantifying the growth and the characteristics according to the relative humidity, The storage stability was investigated every 5 days and freshness was measured by ‘Minamide Method’. S7 pileus diameter is The higher the relative humidity was confirmed becomes larger and the more increased relative humidity also increase the comparison yield ability of 1 cycle. However, pileus diameter or stipe length This could not see the big difference in the three treatment groups, the plieus thickness was no significant difference in the treatment of 80% and 95%. The fresh weight of S7 decreased significantly at 80% and 95% relative humidity after 10 days of storage, but decreased continuity in 65% humiditiry. The elongation percentage of S7 pileus was observe in 95% relative humidity, values of L, a and b (SCI), showed the highest L value in 65% and the a value in 95%. b values were similar in 65%, 80% and 95% treatments. On the ‘Minamide Method’ measure freshness was changed from the 10th day of storage at relative humidity of 95%, but humidity of 65% and 80% treatments, it changed after 15 days of storage. In this study growth characteristics and yield were increased at higher relative humidity, but storage stability was decreased. Therefore, it is deemed necessary to change the relative humidity to produce high quality mushrooms.

The purpose of the study was to determine the effect of joint mobilization with respiratory muscle taping on pulmonary function and chest expansion ability in patients with chronic stroke. Thirty subjects (n=30) were randomly divided into three groups: a joint mobilization group (n=10), a respiratory muscle-taping group (n=10), and a joint mobilization with respiratory muscle-taping group (n=10). Measurements for pulmonary function and chest expansion were performed to assess its effectiveness. A spirometer was used to measure the pulmonary function, and a tape measure was used to assess the chest expansion. In the joint mobilization group, peak expiratory flow (PEF) was increased. Forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and upper and lower chest expansion were also increased. All of variables of respiratory muscle-taping group also were increased. FEV1 and FVC were significantly different between joint mobilization with respiratory muscle-taping and respiratory muscle-taping group. The results of the present study suggest that mobilization with respiratory muscle taping increase the pulmonary function and chest expansion.