The antibacterial and antifungal effect of grapefruit seed extract(GFSE) was investigated for its purpose of application to a diverse spectrum of field as sanitizers, disinfectants and preservatives. GFSE showed comparatively high content of such flavoniods as naringin and hesperidin and ascorbic acid. GFSE containing a low level of organic acids is a heavy viscous and water-soluble liquid. As a result of the antimicrobial test of GFSE, Bacillus subtilis and Aspergillus oryzae did not survive at detectable levels when treated with more than 100 ppm of GFSE. The minimum inhibitory concentrations of GFSE for a wide variety of pathogenic and putrefactive bacteria, fungi and yeasts were 100 ppm and 250 ppm, respectively. In the comparable electron micrograph of microbial cells treated with GFSE or not, we could conclude that GFSE destroy microorganisms by disrupting the functions of the cell wall membrane and microbial spores.

In this study, breakwater model which has several outlet pipes to discharge water is settled in the experimental open channel and mean velocity distributions of multi wall jet are measured. The length of zone of flow establishment of wall jet is shorter than that of free jet and decay rate of jet centerline longitudinal velocity along x is linear in 0.3≤x/lp≤17. The rate of vertical width and lateral width spreading of multi wall jet is respectively 0.0753, 0.157.

Various Eulerian-Lagrangian models for the one-dimensional longitudinal dispersion equation in nonuniform flow were studied comparatively. In the models studied, the transport equation was decoupled into two component parts by the operator-splitting approach; one part is governing advection and the other is governing dispersion. The advection equation has been solved by using the method of characteristics following fluid particles along the characteristic line and the results were interpolated onto an Eulerian grid on which the dispersion equation was solved by Crank-Nicholson type finite difference method. In the solution of the advection equation, Lagrange fifth, cubic spline, Hermite third and fifth interpolating polynomials were tested by numerical experiment and theoretical error analysis. Among these, Hermite interpolating polynomials are generally superior to Lagrange and cubic spline interpolating polynomials in reducing both dissipation and dispersion errors.