To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells.

The fishery compensation by marine spatial planning such as routeing of ships and offshore wind farms is required objective data on whether fishing vessels are engaged in a target area. There has still been no research that calculated the number of fishing operation days scientifically. This study proposes a novel method for calculating the number of fishing operation days using the fishing trajectory data when investigating fishery compensation in marine spatial planning areas. It was calculated by multiplying the average reporting interval of trajectory data, the number of collected data, the status weighting factor, and the weighting factor for fishery compensation according to the location of each fishing vessel. In particular, the number of fishing operation days for the compensation of driftnet fishery was considered the daily average number of large vessels from the port and the fishery loss hours for avoiding collisions with them. The target area for applying the proposed method is the routeing area of ships of Jeju outer port. The yearly average fishing operation days were calculated from three years of data from 2017 to 2019. As a result of the study, the yearly average fishing operation days for the compensation of each fishing village fraternity varied from 0.0 to 39.0 days. The proposed method can be used for fishery compensation as an objective indicator in various marine spatial planning areas.

To investigate the industrial availability of liquid fermentation (PL-ferment) by Phellinus linteus mycelium as a postbiotics for the inhibition of inflammation, PL-ferment was fractionated into culture supernatant (CS), hot-water extract (HW) from PL-ferment, EtOH-precipitate (CP) fractionated from HW, and the dialysate (DCP) of CP. Compared to the other fractions, DCP which is expected to contain exopolysaccharide (EPS) as the major component, significantly decreased the production of NO, IL-6, and MCP-1 in LPS-induced RAW 264.7 cells, and IL-6 and IL-8 in TNF-α and IFN-γ-induced HaCaT cells. The general component analysis results showed that no significant difference in components was observed between the fractions, whereas sugar composition analysis revealed that DCP had decreased glucose and increased mannose contents compared to the other fractions. This suggests that mannose played an important role in the anti-inflammatory activity of the active fraction, DCP. Molecular weight distribution analysis revealed that DCP was mainly composed of low-molecular-weight material-removed high-molecular-weight polysaccharides of 18–638 kDa, suggesting that EPS originated from P. linteus EPS. In conclusion, our results suggest that the DCP of P. linteus mycelium fermentation using the anti-inflammatory activity could be used industrially as postbiotic material.

딸기 하우스 내 해충에 대한 일관된 생물적 방제 시스템을 마련하기 위해 낮은 온도에 발생하는 애못털진딧물(Chaetosiphon minus)에 대한 유용천적을 선발하고, 그 이용가능성을 검토하는 과정에서 방제대상인 애못털진딧물의 생물적 특성을 조사한 결과, 약충의 발육기간은 10°C, 15°C, 20°C, 25°C에서 각각 평균 41.7일, 20.6일, 11.9일, 9.8일이었고, 약충의 발육임계온도는 5.5°C, 온일도는 185DD였으며, 순증식율은 25°C에서 22.38보다 20°C에서 30.16으로 높게 나타났다. 암컷성충의 총산자수는 20°C에서 58일 동안 35.2마리, 25°C에서 36일 동안 26.5마 리로 나타났다. 결과적으로 딸기 하우스 내에서 애못털진딧물이 다른 진딧물류에 비하여 낮은 온도에 적응하고 있음을 알 수 있었고, 저온에 활동성이 높은 꽃등에류와 같은 천적의 상업화가 필요하다고 판단된다.

본 연구에서 흰목이버섯의 추출조건별 추출수율과 기능성 성분의 함량을 비교하여 최적 추출조건을 분석하였으며, 모든 분석에서 R²이 0.9331~0.9462로 유의성을 보이는 것을 확인하였다. 각 독립변수에 따른 추출수율과 ergothioneine, β-glucan 성분을 분석한 결과 추출수율은 추출온도와 추출시료농도에 반비례했으며, 추출시간에는 큰 영향이 없었다. Ergothioneine 성분은 온도가 증가할수록 증가하며, 추출시간은 4.33 h가 가장 높았고 시료량에 큰 영향이 없었다. β-glucan 성분은 온도가 증가할수록 감소했으며, 추출시간에 큰 영향이 없었고 시료량이 21.2 mg/mL에서 가장 높았다. 모든 종속변수의 최대 독립변수는 온도 60 o C, 추출시간 4.33 h, 추출시료농도 16.6 mg/mL에서 추출수율 24.9%, ergothioneine 성분함량 66.8 ug/g, β-glucan 성분함량 34.9 g/100 g으로 나타났다.

This study was designed to evaluate the improvement in exercise endurance by Hovenia dulcis fruit hot water extract (HDWE) for the potential application as a functional food material. The effect was based on the evaluation of the forced-swimming capacity and changes in biochemical parameters in mice. Six-week-old mice were classified into four groups based on a randomized block design: the negative control group, the HDWE 50 mg/kg body weight group, the HDWE 200 mg/kg body weight group, and the 1-octacosanol 50 mg/kg body weight group (n=8 mice per group). The swimming exercise time in the HDWE-50 group was increased at 545±43 sec compared to the control group at 263±28 sec. The HDWE-200 fatigue indicators, lactate acid and ammonia content, decreased to 4.99±0.33 mg/mL and 0.63±0.05 mg/mL, respectively. In addition, the content of glycogen in the liver and muscle tended to increase in the group administered HDWE but was not significant. Likewise, the LDH and glucose contents were increased compared to the control group. The muscle damage indicators ALT (52.7±2.6 U/L) and AST (42.5±5.2 U/L) tended to decrease compared to the control group (76.4±10.2 U/L).

Recently, a number of recent reports have reported cases of bone marrow defect (BMD) in the mandible. Among the diseases seen by BMD in radiographs, focal osteoporotic bone marrow defect(FOBMD) is asymptomatic, which is discovered incidentally and localized radiolucency. Because it varies in size, shape, trabeculae and border definition, it needs to biopsy to differential diagnose other intraosseous lesions that show radiological bone marrow defects. This cases report discusses the pre-operation considerations in patients planning to implant the mandible and suspected to have FOBMD in radiographs. The following 4 cases in Dental Hospital of Wonkwang University were taken in panoramic and Cone beam computed tomography(CBCT). In all cases, there were radiolucent lesion diagnosed with FOBMD under radiologic differential diagnosis. In three of cases the implant were placed without treatment plan change and one case changed the treatment plan with removable partial denture. BMD is anatomical state that can affect primary stability of implant fixture. During implant placement, it can lead to unexpected results that fixture is fallen into BMD and the nerve may be damaged after implant fixture removal to cause hyperesthesia. Preoperative diagnosis of these lesions by differential diagnosis with panoramic and CBCT can prevent the complications by changing the treatment plan or paying attention to the manipulation during surgery.

본 연구는 효과적인 사면복원을 위해 친환경 식생자루의 특성을 분석하였다. 경북대학교에서 개발한 친환경 식생자루와 기존에 국내에서 사용하던 론생백과 황마씨자루를 대상으로 인장강도시험, 내후성시험, 전단강도시험을 통해 식생자루별 특성을 비교분석 하였으며, 친환경 식생자루의 현장적용가능성을 파악하기 위해 상주시 목재문화체험관 뒷 사면에 시공하여 실외발아실험을 진행하였다. 그 결과, 친환 경식생자루가 기존의 식생자루에 비해 200 N 높은 인장강도를 보였으며, 내후성시험에서는 근소한 차이로 기존의 식생자루가 높은 저항성을 보였으나, 강도의 분포가 고르지 않은 경향을 나타냈다. 전단강 도시험에서는 친환경식생자루가 높은 토양응집력과 접촉효율을 나타내어, 현장시공 시 높은 토양안정 효과를 가져 올 것으로 판단된다. 마지막으로 실외발아실험결과, 초본, 관목, 교목 모두 70%의 높은 피복율을 나타냈다. 따라서 친환경 식생자루가 기존의 식생자루에 비해 사면복원 및 녹화사업에 유리한 것으로 분석되었다. 본 연구는 친환경 식생자루와 기존의 식생자루의 특성을 비교함으로써 효과적인 사면복원 방법 및 친환경 재료의 활성화에 대한 기초자료로 활용될 것으로 판단된다.

1. 볼리비아 국토 면적의 34.21%는 농경지로 이 중 89.06% 는 목초지다. 볼리비아 전체인구 중 농촌지역 거주민이 30.7% 인데 반해 차코지역은 과반수(약 58%)가 목축업, 작물재배 등에 종사 중으로 농업이 지역경제의 주요 축을 구성한다. 2. 차코는 생태학적으로 식생이 다양하고 생물다양성이 보전된 곳으로 농업 잠재성은 높은 반면 저개발 되어 있고, 원주민의 한정된 공간에서의 무분별한 재래식 방목과 불충분한 식수와 초지 부족, 기후변화로 토양침식, 생태계 파괴가 진행 되고 있다. 3. 산지축산체계는 나무·관목 및 목초의 선별적 간벌을 통해 임간초지를 조성하고 목초를 생산하며 계획적 방목을 통한 지속가능한 축산관리 모델임이 입증됐다. 4. 체계 도입조건으로 자연·물리적 환경(최소 100ha 이상·수자원 접근 가능), 예산운영(4년간 114,000 Bs 고정지출 능력), 기술적용(가축관리 및 식수 수집·저장), 관리능력(축산관리 기본지식·학습의지)이 주요했다. 5. 농업개발협력 시 발생 문제(예산처리지연, 이해당사자 간 소통 미흡, 참여저조)를 해결하기 위해 지속가능한 사업관리체계 및 거버넌스가 구축돼야 한다.

This experiment was carried out to study the change of productivity and feed value in different types of hilly pasture. The pasture utilized in the experiment was placed on the experimental farm of Pyeongchang Campus of Seoul National University. Forage production type(FP; Orchardgrass 18 + Tall Fescue 12 + Timothy 5 + White clover 5 kg/ha) and Public farm type(PF: Orchardgrass 12 + Tall Fescue 18 + Timothy 5 + White clover 5 kg/ha) pasture were established in September 3, 2014 and utilized (cutting or grazing) four times every year. Growth characteristics, yield and forage quality were investigated for two years. Plant height of grasses was the highest in the 1st cutting and legumes was in the 2nd cutting. Dry matter (DM) content was highest at every the 1st cut grasses significantly lower at the 2nd harvest (p<0.05). However, there was no significant difference in the 2nd, 3rd and 4th harvest in 2016. In the botanical composition change, the portion of legume was gradually increased after pasture establishment and the ratio of weed and bare land was higher at 2nd and 3rd cutting, but it was decreased at 4th harvest. There was no significant difference in 2016 of fresh yield between two farm types (p>0.05). The yield of dry matter showed similar trend of fresh yield and forage production type was higher than that of public farm type (p<0.05). The forage intake by livestock was 1,452 kg/ha in 2015 and 1,743 kg/ha in 2016. Pasture utilization ratio of public farm type pasture was highest in the 3rd grazing time. Forage quality of pasture in relation to management type had not significant difference, but there was difference in harvest times. Crude protein (CP) was the lowest in the 1st harvest and total digestible nutrient (TDN) was highest in the 1st harvest and lowest in the 4th harvest. Based on the above results, it is found that the establishment of pasture suitable for farm’s situation is important for set up of Korean model of hilly pasture. Although the forage production type is superior on forage productivity, it is recommended that the results will be provided as basic data for management of public farm type in the future.

본 연구는 돼지의 정자와 난소내 과립막세포에서 bisphenol S(BPS)가 생존성과 활성산소 생산에 미치는 영향을 알아보고자 연구하였다. 돼지정액은 0, 5μM BPS를 처리하여 3, 6시간동안 배양하였다. 정자의 생존성은 SYBR14/PI를 이중 염색하여 분석하였으며, 활성산소의 생산을 측정하였다. 또한, BPS(0, 5, 10, 20μM)를 과립막세포에 처리하여 24, 48, 72시간동안 처리하였다. 처리 후, 세포의 생존율과 활성산소 생산(단, 5μM BPS)을 측정하였다. 그 결과, 돼지에서 정자의 생존율은 BPS에 의해 감소하였고, 활성산소의 생산은 모든 처리시간에서 증가하였다(p<0.05). 또한 과립막세포의 생존은 BPS에 의해 억제되었고, 활성산소는 유의적으로 증가하였다(p<0.05). 이상의 결과를 토대로, BPS의 노출은 정자의 활성과 번식과 관련된 세포에 나쁜 영향을 미칠 것이다.

A total of 594 reservoirs (17%), which are managed by KRC, equipped with earthquake-resistant facilities whereas remaining ones did not. In addition, reservoirs were placed without the effect of geological structures (i.e., fault and lineament). Therefore, development on technique for alleviating the potential hazards by natural disasters along faults and lineaments has required. In addition, an effective reinforcement guideline related to the geological vulnerabilities around reservoirs has required. The final goal of this study is to suggest the effective maintenance for the safety of earth fill dams. A radius 2 km, based on the center of the reservoir in the study area was set as the range of vulnerability impacts of each reservoir. Seismic design, precise safety diagnosis, seismic influence and geological structure were analyzed for the influence range of each reservoir. To classify the vulnerability of geological disasters according to the fault distribution around the reservoir, evaluation index of seismic performance, precise safety diagnosis, seismic influence and geological structure were also developed for each reservoir, which were a component of the vulnerability assessment of geological disasters. As a result, the reservoir with the highest vulnerability to geological disasters in the pilot district was analyzed as Kidong reservoir with an evaluation index of 0.364. Within the radius of 100km from the epicenter of the Pohang earthquake, the number of agricultural infrastructure facilities subject to urgent inspections were 1,180 including reservoirs, pumping stations and intakes. Four reservoirs were directly damaged by earthquake among 724 agricultural reservoirs. As a result of the precise inspection and electrical resistivity survey of the reservoir after the earthquake, it was reported that cracks on the crest of reservoirs were not a cause of concern. However, we are constantly monitoring the safety of agricultural facilities by Pohang aftershocks.

In this study, performance based seismic design was performed on the shear wall structural system and the beam-column system as a variable general rebar and seismic rebar, and comparing the capacity of the two models of each system. From nonlinear analyses, the capacity of the shear wall structural system applying seismic rebar has shown a stable behavior after the maximum strength, but there is little difference. Furthermore, both models showed similar capacity between story drift and story shear force and capacity of members. These results are attributed to the fact that the seismic rebar, which is highly ductile under the seismic load applied to the target structure, does not render sufficient capacity.

In most mammals, metaphase II (MII) oocytes having high maturation promoting factor (MPF) activity have been considered as good oocytes and then used for assisted reproductive technologies including somatic cell nuclear transfer (SCNT). Caffeine increases MPF activity in mammalian oocytes by inhibiting p34cdc2 phosphorylation. The objective of this study was to investigate the effects of caffeine treatment during in Vitro maturation (IVM) on oocyte maturation and embryonic development after SCNT in pigs. To this end, morphologically good (MGCOCs) and poor oocytes (MPCOCs) based on the thickness of cumulus cell layer were untreated or treated with 2.5 mM caffeine during 22-42, 34-42, or 38-42 h of IVM according to the experimental design. Caffeine treatment for 20 h during 22-42 h of IVM significantly inhibited nuclear maturation compared to no treatment. Blastocyst formation of SCNT embryos was not influenced by the caffeine treatment during 38-42 h of IVM in MGCOCs (41.1-42.1%) but was significantly improved in MPCOCs compared to no treatment (43.4 vs. 30.1%, P<0.05). No significant effects of caffeine treatment was observed in embryo cleavage (78.7-88.0%) and mean cell number in blastocyst (38.7-43.5 cells). The MPF activity of MII oocytes in terms of p34cdc2 kinase activity was not influenced by the caffeine treatment in MGCOCs (160.4 vs. 194.3 pg/ml) but significantly increased in MPCOCs (133.9 vs. 204.8 pg/ml). Our results demonstrate that caffeine treatment during 38-42 h of IVM improves developmental competence of SCNT embryos derived from MPCOCs by influencing cytoplasmic maturation including increased MPF activity in IVM oocytes in pigs.