In ecosystems within limited resources, interspecific competition is inevitable, often leading to the competitive exclusion of inferior species. This study aims to provide foundational information for the conservation and restoration management of Microphysogobio rapidus by evaluating species’ ecological response to biological factors within its habitat. To understand this relationship, we collected food web organisms from site where M. rapidus coexist with Microphysogobio yaluensis, a specie ecologically similar to M. rapidus, and evaluated the trophic levels (TL), isotopic niche space (INS), and the overlap of INS among fishes within the habitat using stable isotope analysis. Our analysis revealed that the M. rapidus exhibited a higher TL than M. yaluensis, with TL of 2.6 and 2.4, respectively. M. yaluensis exhibited a broad INS, significantly influencing the feeding characteristics of most fish. Conversely, M. rapidus showed a narrow INS and asymmetric feeding relationships with other species, in habitats with high competition levels. This feeding characteristics of M. rapidus indicate that the increase in competitors sharing the similar resources lead to a decrease in available resources and, consequently, is expected to result in a decrease in their density.

Purpose: This study aimed to identify and describe the experiences of new nurses trained by clinical nursing educators. Methods: Data were collected through focus groups and in-depth interviews conducted between July 27 and August 12, 2021. The collected data was qualitatively analyzed through content analysis. Results: Six themes and 19 subthemes emerged. The themes included: “Worried about not adjusting to work”, “Not feeling at ease with the preceptor”, “Experiencing the benefits of working with the clinical nurse educator”, “Getting practical support from the clinical nurse educator”, “Receiving mental and emotional support from the clinical nurse educator”, and “Appreciating successful adjustment”. Conclusion: This study confirmed the importance of the educator’s role in helping new nurses adjust to the workplace and develop competence.

We conducted an on-site application study at the livestock cooperative fertilizer plant to compare the composting period, temperature change, moisture content, and chemical properties between livestock manure compost using sawdust as a moisture regulator with those using spent oyster mushroom substrate. The composting period, moisture content, and fertilizer composition of compost containing spent oyster mushroom substrate did not differ from that of conventional compost mixed with sawdust after the first and second fermentation and post-maturation stages, it was suitable as a material for manufacturing livestock manure compost. The spent oyster mushroom substrate also lower the production cost of livestock manure compost by replacing the more expensive sawdust. The developed technology is expected to contribute towards the utilization of by-products of the oyster mushroom harvest while simultaneously producing high quality livestock manure compost.

본 연구는 전라북도 남원시, 장수군과 경상남도 함양군의 경계에 위치한 백두대간 봉화산의 관속식물상을 파악하여, 생물다 양성 보존 및 외래식물의 확산 방지를 위한 기초자료로 활용하기 위하여 조사를 실시하였다. 국립수목원 산림생물표본관에 소장된 봉화산 표본 검토와 함께, 2020년 4월부터 2023년 7월까지 총 15회에 걸쳐 현지조사를 수행하였다. 그 결과, 본 조사 지역의 관속식물은 97과 279속 409종 16아종 32변종 9품종의 총 466분류군으로 조사되었다. 이 중 특산식물은 8분류군, 적색목록 중 취약(VU)에 속하는 종은 1분류군으로 확인되었다. 식물구계학적 특정식물종은 총 57분류군으로 Ⅴ등급에 1분류 군, Ⅲ등급에 9분류군, Ⅱ등급에 14분류군, Ⅰ등급에 33분류군이 각각 발견되었으며, 외래식물은 24분류군, 그리고 생태계교 란야생종은 1분류군이었다. 분류군의 유용성 조사에서 식용 353분류군, 섬유용 3분류군, 약용 71분류군, 관상용 21분류군, 목초용 108분류군, 공업용 2분류군, 잡용 7분류군, 용재용 11분류군으로 나타났다.

The consumption of ready-to-eat side dishes is rapidly growing in South Korea. These foods are particularly vulnerable to microbiological contamination as they are often cooked without any treatment, such as heating or stored at room temperature after cooking. Hence, in 2022, we analyzed the ready-to-eat side dishes sold in Gyeongsangnam-do, South Korea for microbiological contamination. We collected 100 samples from supermarkets in 7 cities, and then examined them for presence of food-borne pathogens and sanitary indicator bacteria. In the analysis of the food-borne pathogens, Bacillus cereus and Clostridium perfringens were isolated from 51 samples (51.0%) and 3 samples (3.0%), respectively. However, both quantitatively met the Korean Food Standards Codex. Genes of five different enterotoxins and one emetic toxin were analyzed from the 51 isolated B. cereus strains. We detected enterotoxin entFM (100.0%), nheA (94.1%), hblC (58.8%), cytK (56.9%), and bceT (41.2%) in 51 isolates, and emetic toxin gene, CER, in only one (2.0%) isolate. We did not detect C. perfringens toxin gene (cpe) that causes food poisoning in any one of the three C. perfringens isolates. In the case of sanitary indicator bacteria, Kimchi had the highest levels of total aerobic bacteria and coliforms, followed by Saengchae, Jeotgal, Jeolim, Namul, and Jorim, respectively. We counted total aerobic bacteria at two different storage temperatures (4oC and 20oC) to determine the effect of storage temperature. When stored at 20oC, total aerobic bacteria count increased in most of the ready-to-eat side dishes, except for Jeotgal. This result conclusively shows the need for refrigerating the ready-to-eat side dishes after purchase. Further research is needed to assess the risk and safety of the ready-to-eat side dishes available in the market and determine appropriate safety management practices.

본 연구는 산림에 대한 보건학적 가치 제고를 위하여 기초적 자료를 제공하기 위해 대학생 20명을 대상으로 난대림과 도시의 경관 감상에 따른 생리･심리에 미치는 변화를 파악하고자 수행하였다. 주요 결과는 첫째, 알파파(RA, RFA, RSA)를 측정한 결과, 좌측 후두엽(O1)이 도시경관보 다 난대림 경관을 감상할 때 더 활성화되었다. 둘째, 베타파(RB, RLB, RMB, RHB)를 측정한 결과, 통계적으로 유의미하지 않았다. 셋째, SEF50과 SEF90은 도시경관보다 난대림 경관을 감상하는 것이 더 안정･이완된 상태임을 나타냈지만 SEF90의 전두엽(Fz)을 제외하고는 유의미하지 않았다. 넷째, 기분상태검사(K-POMS-B)를 수행한 결과, 난대림 경관 감상은 긴장, 혼돈, 피로의 부정적인 기분상태는 감소하였고, 긍정적인 기분상태 요인인 활기는 증가하여 종합기분장애 점수는 도시(0.95±10.860)에 비해 난대림(-5.45±5.463)에서 감소하는 유의미한 결과를 확인하였다.

편백나무(Chamaecyparis obtusa)는 측백나무과에 속하며 우리나라 남쪽 지역에서 주로 잘 자 생한다. 편백나무의 목재는 재질이 우수하여 가구로서의 활용이 높으며 남은 목재, 가지, 잎은 정유 추출에 사용되고 있다. 편백 정유는 항염, 향균, 탈취, 진정 효능이 탁월한 것으로 알려져 있다. 본 연구는 이러한 편백나무의 잎에서 추출한 오일이 주름 개선, 피부 장벽 및 보습능에 미치는 영향을 평가하고자 하였다. 먼 저, DPPH assay와 ABTS assay를 통해 항산화 활성을 평가한 결과, 편백 오일은 두 가지 라디칼을 농도 의존적으로 유의하게 소거하였다. 그리고 주름 생성 억제 효능 평가를 위해 elastase 활성을 측정한 결과, 편백 오일은 elastase 활성을 직접적으로 억제시킴을 확인하였다. 다음으로는 real-time PCR을 통해 유전 자 발현을 확인한 결과, 편백 오일은 인간섬유아세포에서 MMP-1 유전자 발현을 유의하게 감소시켰다. 또 한, 편백 오일에 의해 각질형성세포에서 filaggrin과 HAS-2 유전자 발현이 유의하게 증가함을 확인하였다. 이러한 결과를 종합해보면, 편백 오일은 주름 개선과 피부 장벽 및 보습능 강화에 도움을 주어 항노화 화장 품의 기능성 원료로서 활용될 수 있을 것으로 기대된다.

Recently, Korean government has introduced Multi Metric Indices (MMI) using various biocommunity information for aquatic ecosystem monitoring and ecosystem health assessment at the national level. MMI is a key tool in national ecosystem health assessment programs. The MMI consists of indices that respond to different target environmental factors, including environmental disturbance (e.g. nutrients, hydrological and hydraulic situation of site etc.). We used zooplankton community information collected from Korean lakes to estimate the availability of candidate zooplankton MMI indices that can be used to assess lake ecosystem health. First, we modified the candidate indices proposed by the U.S. EPA to suit Korean conditions. The modified indices were subjected to individual index suitability analysis, correlation analysis with environmental variables, and redundancy analysis among indices, and 19 indices were finally selected. Taxonomic diversity was suggested to be an important indicator for all three taxonomic groups (cladoceran, copepod, rotifer), on the other hand, the indices using biomass for large cladocerans and copepods, while the indices using abundance were suggested for small cladocerans and rotifers.

Broad bean wilt virus 2 (BBWV2) is a species in the genus Fabavirus and family Secoviridae, which is transmitted by aphids and has a wide host range. The BBWV2 genome is composed of two single-stranded, positive-sense RNAs, RNA-1 and RNA-2. The representative symptoms of BBWV2 are mosaic, mottle, vein clearing, wilt, and stunting on leaves, and these symptoms cause economic damage to various crops. In 2019, Perilla fructescens leaves with mosaic and yellowing symptoms were found in Geumsan, South Korea. Reverse-transcription polymerase chain reaction (RTPCR) was performed with specific primers for 10 reported viruses, including BBWV2, to identify the causal virus, and the results were positive for BBWV2. To characterize a BBWV2 isolate (BBWV2-GS-PF) from symptomatic P. fructescens, genetic analysis and pathogenicity tests were performed. The complete genomic sequences of RNA-1 and RNA-2 of BBWV2-GS-PF were phylogenetically distant to the previously reported BBWV2 isolates, with relatively low nucleotide sequence similarities of 76-80%. In the pathogenicity test, unlike most BBWV2 isolates with mild mosaic or mosaic symptoms in peppers, the BBWV2-GS-PF isolate showed typical ring spot symptoms. Considering these results, the BBWV2-GS-PF isolate from P. fructescens could be classified as a new strain of BBWV2.

월출산국립공원 내에 위치한 남생이(Mauremys reevesii) 대체서식지에서 사육 중인 남생이 15개체(암컷 6, 수컷 9)를 대상으로 개체 간 구애와 공격 행동을 관찰하여 사회적 상호작용을 알아보고자 하였다. 이를 위하여 2020년 5월 18일부터 2020년 10월 19일까지 연구지역에 CCTV를 설치하여 매주 3일(24시간/일), 총 64일 동안 녹화하였고 시간 당 10분 간격으로 분석하였다. 남생이의 구애행동은 총 884회 관찰되었고 수컷은 체중이 가장 가벼운 암컷(3회)보 다 가장 무거운 암컷(357회)에게 더 많은 빈도의 구애행동을 하였다. 남생이의 공격행동은 총 259회 관찰되었으며 암컷-암컷 간 30회, 수컷-수컷은 81회, 암컷-수컷은 148회 관찰되었다. 이를 바탕으로 파악한 우열관계는 전반적으로 암컷이 수컷보다 더 높게 나타났으며, 몸 크기가 클수록 순위가 높게 나타났다. 본 연구는 국내 최초로 남생이의 구애행동 빈도를 통해 번식가능성이 높은 개체를 확인하고 공격 행동에 따른 우열관계를 파악하였다. 연구결과는 멸종위기에 처한 남생이의 관리 및 보전을 위한 행동학적 기초 자료로 활용될 수 있으며, 남생이의 사회구조를 이해하는 데 도움이 될 것이다.

스마트농업의 활성화를 위해서는 농업환경 데이터의 관측과 모델 그리고 통계자료의 상호운영성 확보가 중요하므로, 이를 위한 격자체계의 적용방안을 탐색하고자 한다. 국내외 격자체계 표준에 대한 사례조사와 농업분야의 격자표준 적용사례조사를 벤치마킹하고 국립농업과학원과 상위기관의 표준현황을 살펴보고, 농업환경 공간정보의 목록과 분류체계를 구축한 후, 사용자 요구사항을 파악하기 위한 설문을 실시하여 적정한 격자 크기를 선정하였다. 농업환경 공간정보의 공유와 활용을 위하여 국토지리 정보원의 기관표준을 차용하여 군 이하 소규모 지역은 10m, 광역시･도는 100m, 농촌경관 목적으로는 500m, 전국은 1km 격자 크기로 안을 제시하였고 원활한 표준 적용을 위한 전략을 제안하고, 현장 조사 및 관측 결과를 격자로 변환할 수 있도록 오픈소스 기반의 변환 툴을 개발하여 격자표준 적용 용이성과 시간 절약성을 확보하였다.

The effect of five different constant temperatures (18, 21, 24, 27, and 30±1°C) and a photoperiod of 14 : 10 (L : D) h on the reproduction parameters of Spodoptera frugiperda was studied. The longevity of adult female S. frugiperda decreased with increasing temperature (22.4 days at 21°C and 13.9 days at 30°C) but not at 18°C. The pre-oviposition period and oviposition period was the shortest at 30°C compared to the other temperatures. The total fecundity egg count was 887.4, 1,246.4, 1,348.9, 1,154.9, and 1,034.2 at 18, 21, 24, 27 and 30°C, respectively, during its life span. The survival rate of female S. frugiperda decreased rapidly after 13 days at 18°C, after 14 days at 21°C, after 15 days at 27°C, and after 9 days at 24°C, and 30°C. On the third day after the start of oviposition, 50% of the total fecundity was accomplished. In corn fields at less than the 10-leaf stage, the distribution of S. frugiperda egg masses was observed in the middle and lower plant regions, corresponding to 46.8% and 41.4% of the total egg masses, respectively. Egg masses were mostly found on the underside of the leaf blade (abaxial) of corn (66.7%). After releasing S. frugiperda adults on May 12, May 17, May 25, and May 30, the number of eggs per egg mass was 89.9, 88.5, 126.6, and 127.9, respectively. Egg masses of the subsequent generations of S. frugiperda were observed from late June, and the number of eggs per egg mass was 155.8 in late June, 270.7 in early July, and 303.5 in mid-July.

To optimize initial cooling conditions, forced-air cooling was applied to freshly harvested oak mushrooms at 2 levels (0oC for 30 minutes, at 0oC for 1 hour) followed by room cooling at 3 levels (-3oC for 1 day, 0oC for 1 day, 3oC for 1 day). After initial cooling, the oak mushrooms were packaged with PVC film, then held in a storage room at 1oC for 6 weeks. Quality characteristics and percentage marketability were then investigated. As a control, Mushrooms were placed in storage with no initial cooling. The quality factors impacting marketability of fresh oak mushrooms were color change and appearance of decay. Off-odor did not occur or developed only slightly, so it did not affect oak mushroom quality within 6 weeks of low temperature storage. In all treatment groups, the shelf life in which 100% marketability was maintained was up to 3 weeks. At week 5, percent marketability of the 3 treatment groups 1 hour room cooling treatment at 0°C, 1 hour forced air cooling, and control was 100%. 80% In the group that underwent 30 min forced air cooling retained 80% marketability, and the group exposed to 1 day in room cooling at -3oC retained 86.7% marketability. At week 6 of 1oC storage, the marketability ratio was 80% in the 1 day room cooling at 0oC group, 66.7% in the 1 day room cooling at 3oC group, 46.7% in the 1 hour forced air cooling group, and 33% or less in all other treatment groups. Therefore, the most suitable initial cooling parameter to extend shelf-life of oak mushrooms is 1 day of in room cooling at 0oC immediately after harvest.

목적 : 본 연구는 전국 일반계 고등학교 재학생 1, 2, 3학년들을 대상으로 작업치료 인식도 조사를 실시하 여 학년별 작업치료 인식도를 확인하고, 그에 따른 학과 탐색의 시기를 파악하는 데에 목적이 있다. 이 를 통해, 작업치료의 인식도를 향상시키도록 하는 효율적인 홍보방안을 제시하고자 한다. 연구방법 : 전국 17개 지역의 일반계 고등학생들을 대상으로 2021년 11월 22일부터 12월 5일까지 알게 된 경로, 최소한의 교육과정, 국가고시 필수 여부, 대상자의 연령대, 업무 범위, 취업처와 같은 작업치료 인식도와 작업치료(학)과 지원 및 홍보 방법에 대한 질의와 응답으로 구성된 N사의 온라인 설문지 공유 를 통해 실시하였다. 수집된 설문지 총 860부 중 부적절한 응답 153부를 제외한 707부를 분석하였다. 수집된 자료 처리 및 분석은 기술통계량을 이용한 빈도분석을 실시하였다. 결과 : 고등학생의 작업치료 인식의 수준은 48.9%로 나타났다. ‘작업치료 대상자의 연령대’를 학년별로 구 분하여 분석한 결과, ‘모르겠다’를 선택한 비율이 1학년 177명(32.2%), 2학년 157명(30.3%), 3학년 84명(23.6%)으로 학년이 높아질수록 낮아지는 것으로 확인되었으며 학년별로 분석한 다른 작업치료 인 식도 항목 또한 같은 결과를 보였다. 고등학생이 희망하는 학과 홍보 방법으로는 ‘Social Network Service(SNS)홍보 게시물’이 26.2%로 가장 높게 나타났다. 결론 : 일반계 고등학생은 학년이 높아질수록 작업치료 인식도가 높아졌다. 이는 고등학생들이 진로를 위한 학과 탐색을 통해 높아지는 것으로 추론할 수 있으며, 충분한 탐색의 시간을 제공하기 위해 고등학생이 희망하 는 학과 홍보 방법인‘SNS 홍보 게시물’을 활용하면 작업치료 인식개선 등의 효과적일 것이라 사료된다.

Asthma is a chronic inflammatory disease characterized by recurring symptoms, airflow obstruction, and bronchial hyper-responsiveness. The onset of asthma for most patients begins early in life, and current asthma treatment with anti-inflammatory agents can have adverse effects, eventually leading to impaired quality of life. In the pathogenesis of asthma, macrophages and basophils play a vital role during progression. Macrophages not only induce inflammation by secreting inflammatory cytokines but also promote DNA damage and mucus production through nitric oxide (NO) production. Basophils enhance eosinophil recruitment and aggravate asthma through the FcεRIα receptor with high affinity for histamine and IgE. Therefore, in this study, we investigated whether the activation of macrophages and basophils is suppressed by the individual extracts of 28 natural products. RAW 264.7 cells (mouse macrophages) were treated with the natural products in LPS, and 4 natural product extracts resulted in decreased NO production. In β-hexosaminidase assay using RBL-2H3 cells (rat basophils), 19 natural product extracts decreased β-hexosaminidase production. In NO production and β-hexosaminidase assay using macrophages and basophils, 3 natural product extracts (Plantago asiatica, Centella asiatica, and Perilla frutescens var. japonica) significantly inhibited NO production and β-hexosaminidase release. Overall, we examined the inhibitory effects of 28 natural product extracts on macrophage and basophil activity, and the findings demonstrated the potential of natural product extracts for treating asthma and macrophage- and basophil-related diseases.