본 연구는 딸기재배시 전용 고설베드를 사용하지 않고, 일 반 수경재배 시설을 이용하여 코이어 배지를 베드에 올려 재 배하는 방법을 구명하기 위해서 실시하였다. 토마토나 파프 리카를 재배하는 시설재배 베드에 코이어의 칩과 더스트 비율 이 5:5인 코이어 배지 1겹 처리(높이 10cm; A), 2겹으로 쌓은 처리(높이 20cm; B), 코이어의 칩과 더스트 비율이 7:3인 코 이어 배지 1겹 처리(높이 15cm; C)와 대조구로는 딸기 전용 플라스틱 화분(Control) 처리구로 하였다. 생육특성은 코이 어 배지 높이별로는 유의성이 없었고, 플라스틱 화분에서 재 배한 것이 작은 경향을 보였다. 딸기 잎의 광합성율은 처리별 로 14.68－15.76μmol CO2·m-2·s-1로 통계적인 유의성은 없 었고, 뿌리의 근활력은 배지 용량이 컸던 C와 B 처리구가 A 처리구와 대조구보다는 높은 것으로 나타났다. 과장과 과폭 은 각각 4.04－4.13cm와 3.26－3.34cm로 통계적인 유의성 이 없었고, 과장과 과폭 비율은 대조구가 1.27로 A－C 처리 구의 1.23－1.24보다 뾰쪽한 형태이었다. 딸기 1주당 수확과 수는 C 처리구가 4.4개로 가장 적었고, 대조구, A, B 처리구의 6.2－6.5개로 처리간 유의성은 없었다. 상품수량 과수는 A 처 리구가 74개로 가장 많았고, C 처리구가 53개로 가장 적었으 며, 1주당 수량은 A 처리구가 72.38g으로 가장 컸고, C 처리 구가 48.69g으로 가장 작았다. 이와 같은 결과는 딸기재배 시 전용재배 시설을 설치하지 않고, 기존의 토마토나 파프리카 수경재배 시설에서 코이어 배지를 활용하여 딸기재배를 할 수 있다는 것을 나타낸다. 다만 C 처리구에서 수량이 감소한 것 은 칩과 더스트 비율이 7:3으로, A와 B 처리구의 칩과 더스트 비율이 5:5와 다른 것이 원인으로 추정되며 칩과 더스트 비율 에 따른 추가 연구가 필요하다.

본 연구는 권취식 측창을 갖는 아치형 단동 플라스틱 온실 내 강제환기장치 설치 및 운용, 환기 성능 개선방안 등을 제안 하기 위해 온실 내부에 유동팬과 배기팬을 설치하여 강제환기 장치 사용에 따른 온실 내부기온 강하 특성을 정량적으로 조 사하였다. 시험은 3가지 환기 조건(측창, 측창+순환팬, 측창+ 순환팬+배기팬)에서 수행되었다. 각 조건 데이터로거를 이용 하여 환기 시작과 동시에 온실 내외부 기온 및 외부환경 변화 를 측정·기록하였고, 환기 방식별 기온차 변화의 평균값으로 부터 정규기온차를 계산하여 기온 강하 효과를 비교하였다. 오전(11:00－12:00)에는 환기 방식에 상관없이 환기 초반 정 규기온차가 일시적으로 증가했다가 감소하는 것으로 나타났 다. 강제환기장치가 더 많이 사용될수록 최대 정규기온차는 1.158에서 1.037로 감소하였고 최대 정규기온차에 도달하는 시간도 340초에서 110초로 단축되었다. 강제환기장치의 사 용은 정규기온차가 0.8까지 감소하는데 소요된 시간을 1,030 초에서 550초로, 0.6까지는 1,610초에서 915초로, 0.4까지는 2,315초에서 1,360초로, 자연환기의 약 60% 수준으로 감소 시켰다. 오후(14:00－15:00)에는 정규기온차의 증가가 관측되지 않았지만, 환기 시작과 동시에 기온차가 감소하기 시작 했다. 또한 강제환기장치가 더 많이 사용될수록 정규기온차 가 0.8까지 내려가는 시간을 560초에서 345초로, 0.6까지는 825초에서 540초로, 0.4까지는 560초에서 345초로, 약 70% 수준으로 감소시켰다. 따라서, 보다 효과적이고 경제적인 환 기를 위해 강제환기장치는 오전과 같이 열부하가 높은 환경에 서 적극적으로 사용하는 것이 바람직하다고 판단된다.

A field study was conducted to reduce airborne bacteria by supplying active ions to indoor spaces used by vulnerable human groups spending substantial amounts of time in places such as schools and hospitals. In an experiment conducted during school hours (8:00-15:00), the average number of airborne bacteria in classrooms was 345.53 CFU/m3 or more without active ions. However, ion supply reduced the airborne bacteria to an average of 113.23 CFU/m3, indicating an efficiency of 61.61%. As a result of tests in 33 rooms used for surgery in small and middle sized hospitals, ion supply for 2 to 4 hours reduced the average airborne bacteria concentration from 243.88 CFU/m3 to 104.34 CFU/m3, representing a 41.53% reduction. A laboratory test to confirm the ion activity has shown that the mortality rate of E. coli used as a test bacterium increased with exposure time to ions. The initial colony number of E. coli was 251 CFU, but decreased to 4 CFU after 60 minutes of exposure to active ions. Therefore, it was confirmed that the supply of active ions can contribute to the control of airborne bacteria in the indoor environment of schools, hospitals and other public facilities.

The present study was conducted to examine the effect of soybean silage as a crude protein supplement for corn silage in the diet of Hanwoo steers. The first experiment was conducted to evaluate the effect of replacing corn silage with soybean silage at different levels on rumen fermentation characteristics in vitro. Commercially-purchased corn silage was replaced with 0, 4, 8, or 12% of soybean silage. Half gram of the substrate was added to 50 mL of buffer and rumen fluid from Hanwoo cows, and then incubated at 39°C for 0, 3, 6, 12, 24, and 48 h. At 24 h, the pH of the control (corn silage only) was lower (p<0.05) than that of soybeansupplemented silages, and the pH numerically increased along with increasing proportions of soybean silage. Other rumen parameters, including gas production, ammonia nitrogen, and total volatile fatty acids, were variable. However, they tended to increase with increasing proportions of soybean silage. In the second experiment, 60 Hanwoo steers were allocated to one of three dietary treatments, namely, CON (concentrate with Italian ryegrass), CS (concentrate with corn silage), CS4% (concentrate with corn silage and 4% of soybean silage). Animals were offered experimental diets for 110 days during the growing period and then finished with typified beef diets that were commercially available to evaluate the effect of soybean silage on animal performance and meat quality. With the soybean silage, the weight gain and feed efficiency of the animal were more significant than those of the other treatments during the growing period (p<0.05). However, the dietary treatments had little effect on meat quality except for meat color. In conclusion, corn silage mixed with soybean silage even at a lower level provided a greater ruminal environment and animal performances, particularly with increased carcass weight and feed efficiency during growing period.

Probiotic strain is known to regulate the immune system by colonizing in the intestine and interact with intestinal cell receptors of lymphoid tissue. In this study, safety of Streptococcus thermophilus KCTC14471BP and silk fibroin coating effects was evaluated with respect to mucin binding abilities and immune system modulation. S. thermophilus KCTC14471BP was coated with silk fibroin by adding 1% water-soluble calcium and 0.1% silk fibroin. S. thermophilus KCTC14471BP showed the high activities of leucine arylamidase and β-galactosidase. Regarding the antibiotic resistance tests, S. thermophilus KCTC14471BP was susceptible to ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol. S. thermophilus KCTC14471BP coated with silk fibroin showed the improved mucin binding ability from 16.1% to 71.3% and was confirmed to have no cytotoxicity against RAW 264.7 macrophage. S. thermophilus KCTC14471BP coated with silk fibroin showed dose-dependently significant increases in pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. These results suggested that S. thermophilus KCTC14471BP can be expected as a promising probiotic bacteria for applications to food industries such as fermentation or functional foods.

본 연구에서는 2021년 전남, 전북을 대상으로 23개소에서 어류 4종(향어, 비단잉어, 참붕어, 금붕어) 총 1,115마리에 대하여 병원체 감염 여부를 조사하였다. 법정전염병 3종(SVC, Spring viraemia of carp, EUS, Epizootic ulcerative syndrome, and KHVD, Koi herpesvirus disease)을 분 석한 결과, 조사한 모든 시료에서 법정전염병이 검출되지 않아 전라권에서 조사한 잉어과어 류는 청정국 또는 청정지역 선언에 중요한 정보를 제공할 것이다.

PURPOSES : The purpose of this study is to perform traffic flow characteristics analysis for each point of the long-term work zones and to propose an estimated capacity method to support the establishment of traffic flow management strategies for the long-term work zones. METHODS : The study explained the difference in traffic flow characteristics between the short-term and the long-term work zones, and estimated the capacity of the long-term work zones. The analysis data were collected from five points of long-term work zones of the twolane highway. And VDS and DSRC data were applied to validate data. RESULTS : The characteristics of traffic flow at each measurement point in the long-term work zones showed some differences, among which the capacity was estimated as the starting point to be 1,200 pcphpl and the ending point, 1,400 pcphpl. The delay length was estimated by applying the queuing theory based on the capacity of the start point where the bottleneck starts. As a result of verifying the congestion length based on space diagram map analysis, it was determined that the capacity calculation value and estimation methodology presented in this study were appropriate. CONCLUSIONS : The long-term work zones are mixed with different characteristics of roads, so as the capacity values depending on the analysis location. Therefore, it is necessary to select analysis points and methodologies for estimating capacity and delay depending on the purpose of the analysis, such as estimating the maximum queue length or analyzing the maximum travel time. Through this study, it is expected that by providing accurate information on congestion in advance, road users can detour to other roads, and construction officials can adjust the construction plan to minimize congestion in the construction section.

The purpose of this study was to examine the quality characteristics of byeolmijang prepared several roasted whole grain powders (oat, brown rice, black soybean, corn) for eight weeks. As the fermentation progressed, the pH decreased from 6.10~6.12 to 4.48~4.92 and the total acidity increased dramatically from 0.41~0.48% to 1.67~2.24%. There were no differences in the moisture content. The content of reducing sugar decreased, in particular, brown rice sample(C) decreased significantly than the other samples during fermentation. In color, L and b-value decreased all samples, whereas a-value showed a tendency to slightly increase. The total cell counts and lactic acid bacteria revealed an increasing tendency during fermentation. In case of the amino-type nitrogen contents, it increased significantly during the fermentation period, especially control sample (A) showed the highest content significantly. The total polyphenol of all samples increased in the fermentation period. ABTS and DPPH radical scavenging activities also increased, especially corn sample (E) had the highest levels. In by electronic tongue analysis, corn sample (E) revealed higher umami and sourness than the control. So, by adding roasted corn powder, it can enhance function and taste of byeolmijang.

This study was carried out to investigate the effect of side vent heights on temperature and relative humidity inside and outside the single-span plastic greenhouse (W: 7 m, L: 40 m H: 3.9 m) during natural ventilation. Four different heights (120, 100, 80, 60 cm) of the side vent were used as an experimental condition. Variations of temperature and relative humidity inside and outside the greenhouse and the differences between heights were compared by using one-way ANOVA. In the daytime, the difference in temperature between inside and outside the greenhouse was dropped from 14.0°C to 7.1°C as the side vent height increased. The temperature difference in the nighttime was less than 0.2°C regardless of the height. One-way ANOVA on the temperature difference between heights presented that the statistical significance was founded between all of the combinations of height in the daytime. The difference in relative humidity between inside and outside the greenhouse was grown from –13.8% to – 22.2% with a decrease in the side vent height. The humidity difference in the nighttime was less than 1% regardless of the height. One-way ANOVA on the humidity difference revealed that most of the side vent heights showed significance in the daytime but between 100 and 80 cm was not significant. It seemed because the external air became cooler during the experiment with a height of 80 cm. Conclusively, this study empirically demonstrated that the higher side vents resulted in the decrease of differences in temperature and relative humidity between inside and outside the greenhouse, and also the effect of side vent height was statistically significant. This study may be helpful for deciding the height of the side vent effective for controlling temperature and relative humidity in a single-span greenhouse during natural ventilation.

Known for its effectiveness in weight loss and diabetes prevention, Gymnema sylvestre products can be found in the US, Japanese, and Indian markets. However, the recommended dosage or safety of these products has not yet been proven. Therefore, development of an analytical method for detecting the content of Gymnema sylvestre in food products is required. Accordingly, this study proposes an analysis method that can examine Gymnema sylvestre in food using LC-ESI-MS/MS and KASP (Kompetitive Allele-Specific PCR) markers. In LC-ESI-MS/MS, a simultaneous analysis method for gymnemic acid and deacylgymnemic acid was optimized using negative ionization mode, and its validation test was completed for solid and liquid samples. In addition, KASP markers were prepared by finding the specific SNP of G. sylvestre in ITS2 and matK through DNA barcodes. The two KASP markers returned positive FAM fluorescence result when combined with G. sylvestre, and this aspect was confirmed in raw G. sylvestre as well. The applicability of the method was tested on 21 different food and healthy functional products containing G. sylvestre purchased on the internet. As a result, although there was a difference in the ratios of gymnemic acid and deacylgymnemic acid in LC-ESI-MS/MS, the index component was detected in all 21 products samples. In the KASP analysis, 9 products returned positive FAM result, and the rest of the products were found to be containing G. sylvestre extract. This study is the first study to use the dual system of LC-ESI-MS/MS and KASP for the analysis of G. sylvestre. The study has confirmed that these two methods are applicable to the examine G. sylvestre content in food products.

본 연구는 나라꽃으로서 상징성이 높은 무궁화를 실내공간에 도입하기 위하여 실내광원별 경관적 특성을 살펴서 효율적인 도입방안을 모색하기 위해 수행되었다. 공시식물로서 무궁화 품종 중 내음성이 비교적 강하다고 알려진 무궁화 “백단심” 을 선정하였으며 형광등, LED등, 삼파장등, 광섬유 4종류의 광원과 500, 750, 1500, 2,500lux의 광도에서 7개월간 실험하였다. 식물의 경관적 측정요소로 엽장, 엽폭, 초장, 엽수, 낙엽수, 절간장, 엽록소함량 등을 조사하였다. 식물의 생리적 요소로서 광합성률(Pn), CO2흡수율(CO2), 세포내 CO2농도(Ci), 대기중 CO2농도(Ca), 기공전도도(Cs), 증산량(Tr) 등을 측정하였다. 또한 기공수를 관찰하여 광합성이 잘 이루어지고 있는지 확인하였으며 광원별 개화율과 색차계를 이용한 화색을 조사하였다. 실험결과 및 도입방안은 다음과 같다. 첫째, 식물의 경관적 구성요소 중 엽수가 모든 광원에서 통계적 유의성을 보였다. 엽수는 엽장, 엽폭과 함께 수관 형성 및 수형에도 영향을 미치는데 광도가 높을수록 많이 출엽하였으며, 광섬유 1,500lux 하에서 가장 많이 출엽하였다. 엽장 엽폭은 광도가 높을수록 크기가 커지는 경향을 보였다. 둘째, 무궁화는 잎도 특이하지만 꽃이 아름답고 상징성이 있어서 선호되는데 무궁화 “백단심” 은 8월~10월에 개화한다. 꽃을 감상할 목적으로 광원별 광조건을 살펴보면, 형광등 750lux~2,500lux, LED등 1,500lux~2,500lux, 삼파장등 750lux~2,500lux, 광섬유1,500lux~2,500lux로 파악되었다. 가장 효과적인 광원 및 광도는 광섬유 1,500lux~2,500lux인 것으로 나타났다. 셋째, 잎의 신선도는 광합성량 및 잎의 기공의 특성으로도 가늠해 볼 수 있는데 광도가 높을수록 광합성률은 높아졌다. 광원별 기공의 수는 7~28개로 나타났으며 LED등 1,500lux와 광섬유 1,500lux에서 가장 많은 기공수를 보였다. 넷째, 색차계 분석결과 꽃색은 분홍빛 보다는 옅은 Green에 가까우며, Blue에 가까운 색상을 나타내었다. L*(명도)의 값은 90 이상의 수치를 나타내어 명도가 매우 높은 것으로 조사되었다. 최종적으로 생육상태 및 생장량, 개화율을 고려할 때 무궁화 백단심의 최적광원은 광섬유 1,500lux인 것으로 판단된다. 그러나 향후 더 많은 무궁화 품종을 대상으로 적정광원과 적정광도를 파악하고 무궁화 연령별 개화율에 대한 실험을 추가적으로 하여 나라꽃 무궁화의 활용가치 및 활용 영역을 향상할 필요성이 있다.

This study was performed to evaluate the effect of pork belly thickness on physicochemical and sensory characteristics after pan frying. Pork belly with three different thickness (5, 10, 20 mm) from three different parts (A:thoracic vertebrae no. 5-9, B:thoracic vertebrae 10-14, C:lumbar vertebrae 1-6) was pan grilled with electronic grill until it reached an internal temperature of 71℃. Cooking loss, shear force, sensory characteristics, volatile compounds of cooked pork belly were determined. Electric nose analysis was performed to support descriptive analysis and consumer sensory analysis. The cooking loss of pork belly with 20 mm thickness from all positions was significantly low (p<0.05). Shear force increased with increase in thickness (p<0.05). Electric nose identified major seven volatile compounds such as acetonitrile, (Z)-4-heptenal, 2-octanol, linalool, carbon disulfide, methylcyclohexane, n-nonanal to discriminate those samples. The part of pork belly significantly affected the intensity of volatile compounds rather than thickness. In discriminate analysis, the overall acceptability of pork belly (A and B parts) with 20 mm thickness was higher than that of pork belly with 5 mm thickness. In addition, the overall acceptability by consumers for pork belly with 20 mm thickness from A, B, and C parts was higher than the overall acceptability for pork belly with 5 mm thickness. This preliminary study suggests that it is more desirable to use pork belly with 20 mm thickness from part A when cooking on a pan gril compared with pork belly of 10 mm or 20 mm thickness from part B or C in the meat markets and restaurant.