본고는 대한제국시기에 들어온 교회당의 정오 종소리와, 1940년경 종이 강탈 되면서 독주한 사이렌의 변화상을 통해 ‘일제강점기 정오의 소리정경’(사운드스 케이프, Soundscape)을 다룬다. 기존 소리정경연구는 주로 사회적, 정치적 의미 만을 다뤘으나, 소리정경의 체계를 좀 더 명확히 적용하여 소리에 담긴 상징적 의미를 더불어 밝힌다. 교회당의 종소리는 어떤 일상적 소리보다도 큰 소리였다 는 점에서 정신적 권력을 보여주는 ‘성스러운 소음’이다. 교회당의 종소리는 일 상적 소리, 즉 기조음으로서 당대인들의 삶에 새로운 리듬이 됐다. 이는 3.1만세 운동이 종소리를 사용한 원인으로 지목됐다. 사이렌은 정치적 목적에서 정오소 리정경을 강탈하려 한 도구로 다뤄졌다. 전쟁물자를 이유로 종이 강탈된 후, 사 이렌이 교회당의 삼종체계가 그러하였듯이 세 번 울리는 체계로 변하며 새로운 관습이 되려 했던 점 및 사이렌을 통한 천황숭배방침이 유대교적 성격이었다는 점이 근거로 제시됐다. 기조음은 일상적이라 사람들에게 특별하게 인지되지 못 하고, 사라진 후에야 향수되기도 한다. 가령, 윤동주는 종이 사라진 정경에 휘파 람을 호출함으로써 종의 기조음이 담던 정신적, 정치적 영향력을 다시 그렸다.

The oocyte undergoes various events during In vitro maturation (IVM) and subsequence development. One of the events is production of reactive oxygen species (ROS) that is a normal process of cell metabolism. But imbalances between ROS production and antioxidant systems induce oxidative stress that negatively affect to mammalian reproductive process. In vitro environments, In vitro matured oocytes have many problems, such as excessive production of ROS and imperfect cytoplasmic maturation. Therefore, In vitro matured oocytes still have lower maturation rates and developmental competence than in vivo matured oocytes. In order to improve the IVM and In vitro culture (IVC) system, antioxidants, vitamins were added to the IVM, IVC medium. Antioxidant supplementation was effective in controlling the production of ROS and it continues to be explored as a potential strategy to overcome mammalian reproductive disorders. Based on these studies, we expect that the use of antioxidants in porcine oocytes could improved maturation and development rates.

단수수[Sorghum bicolor (L)]는 당류가 풍부한 사탕수수, 사탕무와 같은 주요 바이오연료 작물 중의 하나이다. 단수수 착즙액은 단수수 줄기를 압착하여 추출하며 주요 성분는 glucose와 fructose 그리고 sucrose로 구성되어 있다. 에탄올은 효모발효공정을 통하여 단수수 착즙액으로부터 쉽게 생산할 수 있다. 단수수 착즙액은 당류 뿐만 아니라 질소나 인산과 같은 다양한 무기영양원도 함유하고 있어 종균 배지로 활용성이 높다. 상업적인 에탄올 생산 공정에서 종균배양은 중요한 발효공정 중의 하나이며 공정비용 절감 을 위해 최적화된 배지가 필수적이다. 본 연구에서는 섬유질계 바이오에탄올 생산을 위한 종균의 배지로서 단수수 착즙액의 특성을 평가하였다. 단수수착즙액의 발효기질 적합성을 평가하기 위해 YPD 및 억새 당화 액을 비교하여 실험하였으며, YPD배지 중 yeast extract 와 peptone은 각각 5 g/L의 농도로 사용하였다. 각각의 기질들은 당 농도가 2%, 5%, 10%가 함유되도록 제조하여 발효를 통한 균체농도를 측정하였다. 그 결과 YPD와 단수수착즙액을 배지로 사용하였을 때 발효 24시간 후에 최대 2.5 x 108 CFU/mL 이상의 균 체농도를 나타내었다. 결과적으로 단수수착즙액은 12시간 발효 후에 1.0 x 108 CFU/mL 균체를 생산하여 섬유질계 바이오에탄올 생산을 위한 종균 배지로서 YPD를 대체하여 사용할 수 있음을 확인하였다.

참외 육묘 시설 내에서 우수한 품질의 모종을 생산하기 위한 LED 광원의 이용 가능성을 검토하기 위해 접목 활착 후 20일 동안 광원과 광도를 달리하여 묘소질과 정식 후 생육을 비교하였다. 광원은 청색광(B), 적청색 혼합광원(RB3, RB7)을 이용하였고, 광도(PPFD)는 50, 100, 200μmol·m-2·s-1로 처리하였다. 조명시간은 일출 (7:30) 전 2시간과 일몰(17:30) 후 2시간씩 하루에 총 4 시간을 처리하는 일장연장법을 이용하였다. 참외 지상부 의 생장지표인 접수 길이와 줄기직경은 청색광의 비율이 높을수록 길어지고 굵어지는 경향을 나타내었다. 적청색 혼합광원(RB3)이 다른 광원들에 비해 건물률과 조직의 충실도가 높은 경향이었다. 광합성률은 적색광의 비율이 높을수록 증가하였으며, RB7 200μmol·m-2·s-1처리구에서 5.44μmolCO2·m-2·s-1로 가장 높았다. 정식 후 RB3 200μmol·m-2·s-1 처리구의 초장이 132.3cm로 가장 길었고, 마디수가 22.7개로 가장 많았으며 개화율도 75%로 가장 높았다. 청색광(B) 단독으로 처리한 것보다는 적청색 혼합광원(RB3)으로 처리한 것이 묘소질을 양호하게 하였고, 광도를 200μmol·m-2·s-1로 높이는 것이 우량묘 생산에 유리할 것으로 판단되었다.

The purpose of this study is to investigate the spiritual atmosphere of the church bell’s sound of modern Joseon (1898-1945). This paper will show proof of the connection between March’s first independent movement and the poems of Youngak Lee, Kwangyun Kim, Janghwan Oh; which provides a spiritual response for the wholeness of modern Korea. The freedom of religion came from Joseon’s dynasty and as a result, the western style churches were built with bell towers. At the time, churches were not only a new style of an edifice, but a new sense of sound of a bell. Joseon’s bell is stroke from the outside of its body by wooden mallets in the deep mountain, while western style church bells ring from the inside of its body by a metal clapper on top of private houses. The new sound of bell gives a new sound culture of noon in Joseon. This paper will show evidence of the bell’s Soundscape which symbolizes wholeness and perfection in the period. For example, March’s first movement started with the church bell’s ring. And the poetry also showed the moment of realization by its meeting.

This study was performed to investigate the quality characteristics of lotus root tea (LT) prepared with Gardenia jasminoides powder (GLT) and Rubus coreanus Miquel powder (RLT). The proximate compositions, physicochemical properties, mineral contents, and sensory evaluations of samples were measured. GLT sample showed higher contents of crude protein, crude fat, crude ash, and moisture (p<0.001) as well as pH (p<0.001) compared to LT and RLT. In terms of color, lightness (L) and yellowness (b) of GLT were the highest among the samples, whereas redness (a) of GLT was the lowest. The results of mineral analysis showed that the mineral contents of K, Ca, and Fe of LT were the highest, whereas the content of Mg of GLT was the highest. The results of browning color intensity of all samples showed that LT had the lowest. GLT showed higher contents of water soluble solids than LT and RLT. In the sensory evaluation, the scores of color, flavor, aftertaste, feeling of throat, and overall preference of LT and GLT were significantly higher than those of RLT. From the findings, this study suggests that addition of Gardenia jasminoides powder was effective for preparation of lotus root tea in the aspects of consumer acceptability.

PO 필름 공급이 경북 성주지역 참외 산업에 미치는 영향을 분석하기 위하여 참외 재배면적, 재배 농가수, 수량 및 소득 등을 분석한 결과, PE 필름은 2010년에 비해 2014년도에는 전국, 경북 성주 지역에서 각각 91.5%, 94.9%, 95.2%로 감소한 반면, PO 필름 보급은 각각 342.5%, 500%, 825% 이었다. 2010년에 비해 2015년 재배면적과 농가수는 각각 8.8%, 11.9% 감소하였다. 수량과 생산량은 각각 38.2%, 25.9% 증가하였다. 조수입은 2010년 3,051 억원에서 2015년 4,020 억원으로 1.3배 증가하였고 소득도 2010년 1,936 억원에서 2015년 4,020 억원으로 2.7배 증가하였다. 이상의 결과를 종합 하면 PO 필름으로 교체되면서, 참외 생산성은 증대되고, 작업 노력 및 유해물질 배출은 감소될 것으로 보여진다.

본 연구는 여름철 참외 시설재배시 저압포그 처리에 의한 온도하강 및 과실 품질 향상 효과를 구명하기 위하여 수행하였다. 2015년 7월 26일 10시부터 18시까지 의 하우스 내부의 평균 온도는 무처리구 43.0oC에 비해 저압포그 처리구에서 35.4oC로 무처리구에 비해 7.6oC 정도 낮았다. 8월 13일 과실의 특성을 조사한 결과, 과중은 무처리구의 432g에 비해 96g 더 가볍고. 과육부 당도 11.0oBrix에 비해 1.3oBrix 더 높았으며 색도(a값)도 5.1에 비해 1.5 더 높아 우수하였다. 6월 23일부터 50일간 참외의 품질을 조사한 결과, 기형과율은 무처리구의 42.6%에 비하여 저압포그 처리구에서는 27.3%로 15.3%p 감소하였고, 상품과율은 무처리구의 57.4%에 비하여 저압포그 처리구에서 72.7%로 15.3%p 증가하였다. 10a당 수량은 무처리구의 2,234kg에 비하여 저압포그 처리구에서 26% 증 가하였다. 이상의 결과를 보아, 여름철 고온기 참외 시설 재배시 저압포그 처리로 온도하강 효과가 우수하여 품질이 향상되고 수량이 증가한 것으로 판단되었다.

딸기의 전조재배에 적합한 인공광원을 모색하기 위해 적색과 청색의 LED를 이용하여 설치조건을 검토하였다. 시험에 사용된 인공광원은 LED PAR(PPFD 2~4μmol·m-2·s-1), LED BAR(PPFD 100~120μmol·m-2·s-1), 백열등(PPFD 2~4μmol·m-2·s-1)이고 전조시기는 정화방이 개화하는 시점에 22시~01시까지 3시간동안 연속조명하였다. 무처리보다는 전조처리구가 초장과 엽병장이 길었으며, 그 중 백열등이 가장 많이 생장하였다. 전조처리구에서 지상부의 생체중과 건물중은 LED PAR 처리구에서 생장량 대비 가장 무거웠다. 지상부의 엽면적 생장량에서는 무처리 보다는 전조처리구가 엽면적이 증대하는 경향이었고, LED PAR 처리가 엽면적이 넓었다. 엽록소 함량은 전조 후 60일 경에는 LED PAR를 제외하고는 모두 감소하는 경향이었다. 광원별로 광 환경을 시뮬레이션한 결과, 100W 백열등 배광곡선이 원형에 가까워 전조재배시 하우스 위쪽으로 많이 발광되는 것을 볼 수 있다. LED PAR 타입을 설치했을 때는 직진성이 강한 LED의 특성상 조명의 중심이 40lux 이상이며 좁은 지향각과 낮은 설치 위치로 인해 좁은 지역에 집광되는 현상이 나타나고 조명과 조명사이에 기준조도 이하의 음영지역이 발생 하였다. 따라서 설치 높이는 2m, 설치 간격은 3.75m로 조절하였고, 배치형태는 W타입으로 변경하여 전체 식물에 1.2μmol·m-2·s-1 이상이 조사될 수 있도록 기준 조도와 균제도를 맞추어 최적화하였다.

This paper presents the impact of partial shading on CuInxGa(1-x)Se2(CIGS) photovoltaic(PV) modules with bypass diodes. When the CIGS PV modules were partially shaded, the modules were under conditions of partial reverse bias. We investigated the characterization of the bypass diode and solar cell properties of the CIGS PV modules when these was partially shaded, comparing the results with those for a crystalline silicon module. In crystalline silicon modules, the bypass diode was operated at a partial shade modules of 1.67 % shading. This protected the crystalline silicon module from hot spot damage. In CIGS thin film modules, on the other hand, the bypass diode was not operated before 20 % shading. This caused damage because of hotspots, which occurred as wormlike defects in the CIGS thin film module. Moreover, the bypass diode adapted to the CIGS thin film module was operated fully at 60% shading, while the CIGS thin film module was not operated under these conditions. It is known that the bypass diode adapted to the CIGS thin film module operated more slowly than that of the crystalline silicon module; this bypass diode also failed to protect the module from damage. This was because of the reverse saturation current of the CIGS thin film, 1.99 × 10−5 A/cm2, which was higher than that of crystalline silicon, 8.11 × 10−7 A/cm2.

The purpose of this study was to investigate the dynamic balance and activity of internal oblique muscle, multifidus muscle, gluteus maximus muscle, biceps femoris muscle during the Y balance test following the wearing of pelvic compression belt. Forty healthy adults were recruited for this test. The dynamic balance score was estimated as the following: (anterior+posteromdial+posterolateral)/(3×leg length)×100. The electromyography signals were measured through %reference voluntary contraction, which was normalized by reference voluntary contraction of Y balance test without wearing the pelvic compression belt. The paired t-test was carried out to compare the dynamic balance score and the activity of the trunk and hip extensor with and without the wearing of pelvic compression belt. The dynamic balance score of the Y balance test when wearing pelvic compression belt was significantly than when measured without wearing the pelvic compression belt (p<.05). The muscle activity of the internal oblique and the multifidus was significantly decreased when wearing pelvic compression belt (p<.05). The muscle activity of the gluteus maximus was significantly increased when wearing pelvic compression belt (p<.05). However, there was no significant difference in hamstring muscle activity, with or without wearing the belt (p>.05). In conclusion, this study shows that the wearing of pelvic compression belt affects trunk muscle and hip extensor muscle activity related to the pelvic mobility and stability and increases dynamic balance and also contributes to the stabilization of the external pelvic stabilization.

Flat panel display devices are mainly used as information display devices in the 21st century. The worldwide waste flat panel displays are expected at 2-3 million units but most of them are land-filled for want of a proper recycling technology More specifically, rare earth metals of La and Eu are used as fluorescent materials of Cold Cathode Flourscent Lamp(CCFL)s in the waste flat panel displays and they are critically vulnerable and irreplaceable strategic mineral resources. At present, most of the waste CCFLs are disposed of by land-filling and incineration and proper recovery of 80-plus tons per annum of the rare earth fluorescent materials will significantly contribute to steady supply of them. A dearth of Korean domestic research results on recovery and recycling of rare earth elements in the CCFLs prompts to initiate this status report on overseas research trends and noteworthy research results in related fields.

Solar cells exhibit different power outputs in different climates. In this study, the temperature dependence of open-circuit voltage(V-oc), short-circuit current(I-sc), fill factor(FF) and the efficiency of screen-printed single-crystal silicon solar cells were studied. One group was fabricated with homogeneously-doped emitters and another group was fabricated with selectively-doped emitters. While varying the temperature (25, 40, 60 and 80˚C), the current-voltage characteristics of the cells were measured and the leakage currents extracted from the current-voltage curve. As the temperature increased, both the homogeneously-doped and selectively-doped emitters showed a slight increase in I-sc and a rapid degradation of V-oc. The FF and efficiency also decreased as temperature increased in both groups. The temperature coefficient for each factor was calculated. From the current-voltage curve, we found that the main cause of V-oc degradation was an increase in the intrinsic carrier concentration. The temperature coefficients of the two groups were compared, leading to the idea that structural effects could also affect the temperature dependence of current-voltage characteristics.

본 연구는 저온기 시설참외 재배 시 탄산가스 발생제(탄산솔)의 사용효과를 구명하기 위하여 수행하였다. 600 m2 크기 하우스에 탄산가스 발생제(100g/1봉)를 10, 20 및 30봉을 각각 매달아 무처리와 비교하였다. 그 결과 무처리구에 비해 처리구에서 탄산가스 농도가 3.0~3.2배 정도 높았다. 그리고 무처리구에 비해 처리구에서 과중이 20.2~22.0g 더 무겁고, 태좌부 당도가 1.5~2.1oBrix 더 높았으며, 색도(a값)도 우수하였다. 또한 탄산가스 발생제 처리한 것이 무처리에 비해 발효과율 및 기형과율이 각각 2.9~3.9%, 5.4~7.3% 감소하였고, 상품과율은 8.7~10.3% 증가하였다. 10a당 상품과 수량은 무처리구의 385.8kg에 비하여 탄산가스 발생제 10, 20 및 30봉 처리 한 것이 각각 10.3%, 14.8%, 16.2% 증가하였다. 이상의 결과를 보아, 저온기 참외 시설재배시 탄산가스 발생제를 시용함으로써 탄산가스 농도가 높아져 광합성이 촉진되어 품질이 향상되고 수량이 증가한 것으로 판단되었다.

This study is conducted to investigate the effects of Chinese artichoke powder on the antioxidant activities and the quality characteristics of tofu. Tofus are being prepared with different amounts of Chinese artichoke powder in ratios of 0, 0.2, 0.4, 0.6 and 0.8% according to the soy milk quantity. According to the increasing contents of Chinese artichoke powder, the yield and turbidity of tofus are significantly increased (p<0.001) whereas the pH levels of tofus are significantly decreased (p<0.001). In terms of color, the L, a and b values are significantly decreased with increasing Chinese artichoke powder concentrations (p<0.001). From the texture profile analysis of the tofus, the chewiness, gumminess and cohesiveness of tofus are significantly decreased (p>0.01) according to the levels of added Chinese artichoke powder content. The contents of isoflavones such as daidzin and genistin are significantly increased with increasing concentrations of Chinese artichoke powder (p<0.001). The microstructure of tofus is being examined by SEM (Scanning Electron Microscope). In the sensory test, the sample which contains 0.4% of Chinese artichoke powder is ranked significantly higher (p>0.001) than the other groups according to several sensory parameters such as appearance, color, flavor, taste, texture and the overall preferences.

집중은 관련된 사건을 선택적으로 주의하고, 관련 없는 사건을 무시하는 인간의 중요한 인지 기능중의 하나이다. 인간의 집중 능력을 관리 이용하는 컴퓨터 기반 장치에 있어서 집중과 비집중 상태를 구분하는 것은 필수적으로 요구되는 조건이다. 본 논문에서는, 뇌파신호로부터 분류기의 입력으로 사용되는 특징을 효율적으로 추출하기 위하여 비선형 반복 패턴 분석기법과 스펙트럼 분석 기법을 새로이 결합하였고(13개 특징 추출), 서포트벡터머신, 역전파 알고리즘, 선형분리, 로지스틱 회귀 분류 기반 분류기들을 포함하는 집중-비집중 분류기들의 성능을 분석하였다. 그중에서 81 %의 정확도를 보이는 서포트벡터머신 분류기가 가장 좋은 성능을 보였다. 또한 스펙트럼 분석으로 추출한 특징만을 사용하였을 경우(76 % 정확도)가 비선형 분석 방법으로 추출한 특징만을 사용했을 경우(67 % 정확도)보다 좀 더 우수한 성능을 보였다. 비선형-스펙트럼 분석법을 복합 적용한 서포트벡터머신 분류기가 추후 집중 관련 장비 설계에 있어서 효율적으로 적용될 수 있을 것이다.

감귤류의 껍질은 플라보노이드의 중요한 소스 중의 하나로서 동아시아에서 내장 및 염증성 질환을 치료하는 민간 의약품으로서 사용되어 왔다. 본 연구에서는 하귤 (C. natsudaidai) 껍질에 포함되어 있는 플라보노이드 성분을 고성능액체크로마토그래피-이중질량분석법 (HPLC-MS/MS)으로 10 개성분을 동정하였다. 플라바논, 플라본 및 쿠마린 유도체는 각각 hesperetin, noviletin 및 coumarin을 사용하여 유효화 하였으며 유효화된 방법으로 정량하였다. 상관계수 (r2)는 > 0.9970으로서 높은 값을 보여주었다. LOD는 >0.01 mg/L이었으며 LOQ는 >0.05 mg/L이었다. 플라보노이드의 총량은 9229.7 ± 0.5 mg/kg 이었다. Naringin의 량이 5010.0 ± 4.5 mg/kg으로 가장 많았으며 sinensetin의 량이 0.6 ± 0.1 mg/kg으로 가장 적었다. 항산화력을 25 μg/mL 에서 500 μg/mL의 농도범위에서 DPPH·, ABTS·+, NO· 소거능 및 FRAP의 항으로 분석하였다. 감귤 플라보노이드의 항산화 능력은 시료의 량이 증가하면 증가함을 알 수 있다.

This paper delves into the recent 'paintings' of African-American artist David Hammons, which combine rubbish-like plastic wraps with the abstract-expressionist style paintings. In straddling between rubbish and art object, his works tend to blur the boundary drawn between two opposite categories in value, art and garbage, provoking the sophisticated taste of Upper-East-side white community in Manhattan, New York. Choosing the venue of his exhibition at a commercial gallery, Hammons's creative efforts is also a critique of what can be seen as the dominance of abstract expressionism and white elitism in American art history. The artist is known for his use of unconventional materials in art making such as black hair, barbecue bones, and elephant droppings, ones that are often associated with African-American experiences in all different levels. Since his debut in the art scene in the 1970s, Hammons has pursued the view of art-making as a medium for provoking contentious issues of racial relations in the States. On the other hand, the reception of Hammons's work as African-American art can be potentially quite limiting, overlooking as it does multi-faceted meanings of his art practice. His unconventional approach to art often took him outside art galleries and museums, where he was seen using a variety of common materials for site-specific installations and performances. Staged in different parts of Manhattan, these acts of art making traverse seemingly opposite communities and cultures, often blurring their boundaries. Hammons's artistic practice can label him what Abdul Jan Mohamed calls "specular border intellectual", revealing as it does the symbiosis of binary oppositions that is basic to the experience of communnal living.

This paper briefly charts the history of excrement as part of the late 20th-century art and explores ways in which excrement functions in the realms of 'High' art. From Piero Manzoni's <Merde d'artiste> to David Hammons' performance <Pissed Off>, excrement has taken a small yet distinctively important part in the development of contemporary art. In an attempt to challenge the hegemony of 'high' art, on the one hand, and resist the commercialization and fetishization of art, on the other, Manzoni allegedly offered his own "shit" preserved in a tin can and sold it at the price of gold of the same weight. Andy Warhol took the legendary Abstract-Expressionist painter Jackson Pollock as the object of parody, simulating Pollock's dripping action by pissing onto the canvas that had been primed with copper-based paint. Warhol's urination produced splashes and stains of iridescent colors just as the patterns on ordinary abstract paintings. In contrast to Pollock's masculine action, Warhol's pissing alludes to the artist's homosexuality. Excrements in art also provoked controversies, debates, and even acts of vandalism against the artworks. The works of Andres Serrano and Chris Ofili infuriated many Christians for the blasphemous use of excrement with religious icons. Politicians engaged in the heated debates on the use of public and national funds in support of some of the 'politically incorrect' contemporary art. In the midst of media sensation and criticisms, these works challenged the conventional understanding of artistic beauty. The preexisting artworks were also targeted. African-american artist Hammons assumed the role of spectator in <Pissed Off> by urinating on Richard Serra's sculpture in the street of New York City. It was an act condemnation levelled at the racist pattern of the way in which large portions of funds and commisions of "public" art tended to promote established 'white' artists, whose work or creative process often failed to reflect the actual public. The use of excrement in art is not unusual in contemporary art practices. With its subversive power, excrement plays an important critical roles in the shaping of contemporary art.

Spermatozoa sorted by flow cytometry have been successfully used to produce offspring in domestic animals and are commercially available for cattle. Also sheath fluid is the important environment for viability of sex-sorted sperm in flow cytometry. The aim of this study was to investigate whether or not HEPES (N-2-hydroxyethylpiperazine-N'-2-Ethanesulfonic acid) has any effect on the viability in sex-sorted Hanwoo (Korean native cattle) sperm. In this study, the semen was collected from Hanwoo of Hoengseong Livestock Cooperation by artificial vagina method then pooled and subjected to cryopreservation in straws. Sperm were cultured for 0, 30, 60 and 120 min with 0, 2.5, 5, 7.5 and 10 mM of HEPES added to the sheath fluid and incubated at 4, 20 and 38, respectively. For the cytometric analysis the frozen-thawed semen was extended with 5 mM HEPES extender to final concentration ( spermatozoa) at 4, 20 and 37. Sperm viability was assessed with SYBR-14 and propidium iodide (PI) staining. This study shows that the viability of sperm was decreased with prolongation of incubation time in all of test. But the viability of sperm which were treated with 38 was gently decreased than that of treated with other temperature. The viability of the control was sharply decreased (p<0.05) than all of the HEPES treatment group at 60 to 120 min in 38. X-sexed sperm was more sensitive than Y-sexed sperm to temperature during f10w cytometry (p<0.05). In conclusion, the results of this study suggest that the sheath fluid with 5 mM HEPES has effect on maintenance of viability after sperm sexing at 37 in Hanwoo.