In this study, this researcher measured the indoor concentration of radon in elementary schools located in Chungcheongnamdo, and conducted a questionnaire survey from June 2008 to June 2011. Indoor radon densities of elementary schools by season were 86.4 Bq/m3 in winter, 71.2 Bq/m3 in fall, 61.1 Bq/m3 in spring, and 40.5 Bq/ m3 in summer in order. Among flooring materials by construction material, the radon level of concrete was 57.8 Bq/ m3, and cement was 71.5 Bq/m3. For exterior wall materials, it was established that the density of cement, concrete, wood, and soil was 102.9 Bq/m3, 64.4 Bq/m3, 51.0 Bq/m3, and 48.7 Bq/m3, respectively. In addition, for radon densities according to distances between a detector and floors, 150 cm and under was recorded at 99.3 Bq/m3, 151 to 200 cm was recorded at 62.6 Bq/m3, and 201 cm and more was recorded at 59.2 Bq/m3 sequentially. From the results of analyzing correlations between radon concentrations and factors affecting the indoor radon concentrations in elementary schools, it was discovered that the nearer the distances to floors were and the older the construction was(r = 0.300), the higher were indoor radon concentrations. With regard to factors influencing the indoor radon concentrations in elementary schools, derived from multiple regression analysis, it was revealed that distances from floors has the greatest influence(β = 0.354, p < 0.05). And it was determined that the construction year was also a factor contributing to indoor radon levels. This had an explanation power of 27.9%.

Indoor pollutants seem to have a negative affect on people from vulnerable households who are considered to be weak by the department of environmental health, such as the elderly, infants, children, patients, and pregnant women. In this study, the concentrations of pollutants were measured, especially those pollutants that endanger vulnerable households such as PM10, HCHO, TVOCs, TBC and fungi. The measurements were taken from September to October of 2013 in the Chungnam area. The results were as follows: PM10 33.2 μg/m3, HCHO 20.35 μg/m3, VOCs 132.30 μg/m3, TBC 562.71 CFU/m3 and fungi 362.04 CFU/m3. In the case of TBC and fungi, there were excessive concentrations at 30.9% and 49.3% compared to the standards set by WHO. In the case of fungi, buildings built before the 1980s showed a concentration of 446.23 CFU/m3, those built in the 1980s and 1990s had a concentration of 320.21 CFU/m3 and those built in the 2000s had a concentration of 313.43 CFU/ m3 indicating that fungi concentration was higher when the building was older. The correlation analysis between indoor air pollutants and factors affecting indoor concentrations indicate that HCHO (r = 0.423), TVOCs (r = 0.329), and humidity (r = 0.359) showed a significant difference (p < 0.01). The current study focused on vulnerable households that were unable to protect themselves from indoor pollutants and where poor living circumstances prevailed such as households that do not receive much sunlight; thus, more attention and focus needs to be given to these particular households

본 연구 는 뮤지컬 관객의 SNS 사용에 있어 자기 결정성 이론의 중요한 세 가지 변수인 자 율성, 유능성, 관계성이 관객 만족과 SNS 재이용에 미치는 영향에 대한 인과관계를 설 명하기 위한 것이다. 이를 위해 SNS를 이용하는 뮤지컬 관객을 대상으로 설문조사를 진행하 였으며, 정보 검색 및 공유 역량(자율성)과 정보 전달 역량 및 타인에 대한 영향력(유능성), 커 뮤니티 보유와 지속적 관계(관계성)를 물어 이러한 요인들이 관객 만족과 SNS 재이용에 영향 을 미치는지를 살펴보았다. 연구 결과 자기결정성의 세 가지 요인인 자율성과 유능성, 관계성 은 모두 관객 만족에 유의한 영향을 미치는 것으로 나타났으며, 그 가운데 관계성이 더 큰 영 향력을 보이고 있음을 밝혀냈다. 또한 관객 만족은 SNS 재이용에 매우 유의한 영향을 미치는 것으로 나타났다. 결과적으로 본 연구는 뮤지컬 관객의 지속적인 동기 부여를 위한 SNS 활용 마케팅 전략을 모색하는 데 근거자료로 활용될 수 있으리라 본다.

In order to determine an authenticity of food ingredient, we used DNA barcode method by universal primers. For identification of animal food ingredients, LCO1490/HCO2198 and VF2/FISH R2 designed for amplifying cytochrome c oxidase subunit1 (CO1) region and L14724/H15915 for cytochrome b (cyt b) region on mitochondrial DNA were used. Livestock (cow, pig, goat, sheep, a horse and deer) was amplified by LCO1490/HCO 2198, VF2/FISH R2 and L14724/H15915 primers. Poultry (chicken, duck, turkey and ostrich) was amplified by LCO1490/HCO 2198 and VF2/FISH R2 primers. But, Fishes (walleye pollack, herring, codfish, blue codfish, trout, tuna and rockfish) were only amplified by VF2/FISH R2 primers. For plant food ingredients, 3 types of primers (trnH/ psbA, rpoB 1F/4R and rbcL 1F/724R) have been used an intergenic spacer, a RNA polymerase beta subunit and a ribulose bisphosphate carboxylase region on plastid, respectively. Garlic, onion, radish, green tea and spinach were amplified by trnH/psbA, rpoB 1F/4R and rbcL 1F/724R. The PCR product sizes were same by rpoB 1F/4R and rbcL 1F/724R but, the PCR product size using trnH/psbA primer was different with others for plants each. We established PCR condition and universal primer selection for 17 item's raw materials for foods and determine base sequences aim to PCR products in this study. This study can apply to determine an authenticity of foods through making an comparison between databases and base sequences in gene bank. Therefore, DNA barcode method using universal primers can be a useful for species identification techniques not only raw materials but also processed foods that are difficult to analyze by chemical analysis.

In this study, the experimental method has been investigated using molecular biological way to identify raw materials from seasoned red-pepper sauce which is one of the most popular spices in Korea. 6 kinds of seasoned red-pepper sauces were chosen as a sample containing chilli pepper, garlic, onion as a major ingredient and species specific primers were used for the identification of the raw material of processed food. Selected samples were pre-treated to remove salt (samples were washed with distilled water 3~4 times for desalting), after that, to amplify the extracted genes, whole genome amplification (WGA) kit was performed. Afterwards, PCR products were confirmed through the electrophoresis. As a result, 102, 180, 280 bp of specific PCR products were confirmed for each major ingredients such as chilli pepper, garlic, onion. From this study, the gene extraction method was validated for the identification of ingredients from the spices and it would be applied to distinction of low quality chilli pepper powder including seasoned red-pepper sauce illegally.

In this study, effective gene extraction methods were compared to identify raw materials of processed meat products through molecular biological methods. Species specific primers were used to identify ingredients of processed foods and, as a sample, 13 kinds of processed meat products including beef, pork and chicken. According to the type of sample, 13 kinds of samples were classified into liquid type, source type and powder type. The samples were pre-treated (centrifugation) and (or) performed Whole Gene Amplification (WGA) kit for amplification of the extracted DNA. As a result, it was possible to identify the raw material of products through the centrifugation of sample 1 ml for liquid type of processed meat products. For source type of products after gene extraction, it was required to perform WGA for the identification of ingredients. For powder type products did not required any further pre-treatment and WGA. In this study, it was an opportunity to confirm the possibility of identification of raw material from the gene extraction of processed meat products and this method could be used to examine the authenticity of raw material of products.

In this study, a method was developed using molecular biological technique to distinguish an authenticity of meats for processed meat products. The genes for distinction of species about meats targeted at 12S or 16S genes in mitochondrial DNA and the species-specific primers were designed by that PCR products' size was around 200bp for applying to processed products. The target materials were 10 species of livestock products and it checked whether expected PCR products were created or not by electrophoresis after PCR using species-specific primers. The results of PCR for beef, pork, goat meat, mutton, venison, and horse meat were 131, 138, 168, 144, 191, and 142 bp each. The expected PCR products were confirmed at 281, 186, 174, and 238 bp for chicken, duck, turkeymeat, and ostrich. Also, non-specific PCR products were not detected in similar species by species-specific primers. The method using primers developed in this study confirm to be applicable for composite seasoning including beefs and processed meat products including pork and chicken. Therefore, this method may apply to distinguish an authenticity of meats for various processed products.

The purpose of this study was to develop slacks patterns for middle-aged abdomen-obese adult males by using the 3D virtual-twin and virtual-garment simulation system. The criteria for subjects in this study were males who had over 25kg/m2 of BMI, over 90cm of waist, and over 0.90 of WHR. A total of 211 adult males who met these criteria were enrolled. The results were as follows: first, a new slacks pattern considerate of abdomen-obese men was development. The basic numerical formula were as follows: front and back hip girth H/4+3.5, front waist girth W/4+1+0.5, back waist girth W/4+1－0.5, front crotch extension H/16, back crotch extension H/8－0.5, front pleats amount 2.7, and back dart amount 1.5. Second, according to the results of the new slacks patterns appearance evaluation, the new slacks pattern scored more highly than the existing pattern in silhouette and ease amount, confirming that the new slacks pattern is appropriate for the abdomen-obese men. Also, the new slacks pattern was evaluated allowing proper space length of waist, abdomen and hip. Virtual models of production through data from a 3D body scan, pattern draft and virtual garment digital program were applied to a prototypic design method in order to enhance the fitness of ready-made garments. The use of the virtual twin made it impossible to comprehend the appearances and ease correspondent to motions. In order to evaluate wearing fitness, therefore, the system should be improved so as to change arm positions and perform various motions.

We measured indoor, outdoor, and personal exposure concentrations of ozone (O₃) at 54 households in Gwangyang from June to September, 2009. The results are as follows. The concentration of ozone in outdoor, 16.53±5.72 ppb, was observed two times higher than the concentration of ozone in indoor, 8.22 ± 4.60 ppb. The similar arithmetic average ozone were obtained in exposed and controlled areas, 15.16 ± 1.45 ppb and 15.25 ± 1.94 ppb, respectively. The ozone concentration of an individual smoker was 24.89 ± 7.11 ppb, which was higher than that of a non-smoker, 21.01 ± 11.79 ppb. The indoor occupant density elevated ozone concentrations. The relatively low concentration of ozone was observed in a house with air conditioner (8.59 ± 4.04 ppb) than a house without the unit(10.07 ± 6.43 ppb).

본 연구는 시설양묘 용기에서 직근성 수종인 찰피나무 유묘의 생육상황 및 뿌리발달 형태를 탐구하기 위하여 5개월 간 플라스틱 온실에서 수행되었다. 공시용기로 사용된 플라스틱 망 용기와 일반 플라스틱 용기에서 생육한 용기묘의 수고생장 차이는 크지 않았으나, 근원경 생장은 플라스틱 망 용기에서 높게 나타났다. 플라스틱 망 용기는 묘목의 뿌리발달에 영향을 끼쳐 묘목의 뿌리발달을 촉진시켰으며, 전체 지하부의 건물생산량을 증가시켰다. 특히 플라스틱 망 용기묘에서는 적극적으로 유도된 공기단근에 의하여 나선형 뿌리가 나타나지 않았다. 따라서 본 실험에 사용된 플라스틱망 용기는 찰피나무 용기묘 생산에 유용하게 활용할 수 있을 것으로 판단되며, 이 용기에서 생산된 묘목은 다양한 식재지 환경에서 식재될 경우 활착율 증가는 물론 생장율 증가도 보일 것으로 생각된다.

Background: This study aimed to investigate the flowering and fruits formation characteristics of Korean Panax ginseng varieties. The results will provide basic data for ginseng seed production and breeding program. Methods and Results: The characteristics investigated included flowering date, seed setting rate, seed type and seed production. The ginseng variety Chungsun had the earliest emergency and flowering dates, whereas Sunhyang showed late emergence and flowering dates. The emergence date of Chunpoong was not later than that of the other varieties, but the flowering date was delayed. The seed setting rate was 64.6%, 75.8%, 78.5% and 74.4% for three, four, five and six-year-old varieties, respectively. The ratio of double seed (RD) for Sunhyang and Chungsun were higher than those for the others, whereas the RD for Chunpoong was the lowest. Yunpoong and Sunone had many stems per plant and a high seed production rate. Seed production was 21.7, 67.7, 74.4 and 89.0㎏/ 10 a in three, four, five and six-year-old varieties, respectively. Conclusions: The emergence date ranged from April 15 to 25, and the flowering date was from May 10 to the 19 for the new ginseng varieties. The average seed multiplication of the ginseng varieties was about 8.5 and 21.1 times a year for varieties in which, seed-production occurs once a year for over four years and four times over six years, respectively.

Background : Water uptake and flow across cellular membranes is a fundamental requirement for plant growth and development, and plant water status is important not only for plant growth under favorable conditions but also for ability of a plant to tolerate adverse environmental conditions. Thus identification of plasma membrane water channel genes (aquaporins) in ginseng provides extensive information for functional studies and the development of markers for salinity stress tolerance. Methods and Results : For salinity treatment, the plants were grown for 4 weeks in culture medium gelled with 0.8% Phytoagar, and the old media were replaced with the fresh medium containing NaCl at 0, 50, 100, 200 and 400 mM, respectively. The samples for stress treated and non-stressed plants were collected from 6h to 72h, and frozen immediately into liquid nitrogen. According to the sequence information from the assembled transcripts, four primer pairs were designed from the aquaporin gene regions. In order to determine the pattern of aquaporins expression in ginseng seedlings to salinity stress, we conducted semi-quantitative RT-PCR. Conclusion : A tonoplast intrinsic protein 1 (TIP1)-type aquaporin is not only believed to be essential for plant life, but also to be beneficial for growth under salinity stress. Therefore, a deeper understanding of aquaporin genes in ginseng will be essential for crop improvement, which could help us to understand the molecular genetic basis for the ginseng genetic improvement and also provide the functional genetic resources for selective breeding and transgenic research.

Background : This study was carried out to understand the effect of seedling weight (SW) on growth and flowering in Panax ginseng. Methods and Results : The testing materials were Chunpoong (CP), Yunpoong (YP) and Jakyeongjong (JK). The increase of seedling (1yr) weight led to an increase in ratio of flowering plant and in number of flower per plant. The seed setting rate of two year-old plant (CP, YP, JK) increased with increase of SW at the planting time (PT) and number of flower per plant of three year-old plant (CP, YP) increased also. In the two year-old plant (JK), the ratio of three leaves per plant was 8.8, 19.6, 31.0, 42.0, 44.7 and 58.2%, respectively, in the SW of >0.6, 0.6~0.8, 0.8~1.0, 1.02~1.2, 1.2~1.4 and 1.4g<. The growth of ginseng plant was good with increase of SW at the PT. Conclusion : There was a highly positive correlation between seedling weight and flowering characteristics.