In order to develop of practical model of the sawdust cultivation of Lentinula edodes, we conducted experiments of the sawdust cultivation in different conditions of cultivation environment and shapes of sawdust medium. For different types of cultivation environments, we provided environmental control system facility, vinyl house and forest cultivation. We also chose the 2.6kg cylindrical type and the 1.5kg cylindrical type for shapes of medium. We decided cultivation on shelves for the 2.6kg cylindrical type sawdust medium and cultivation on the ground for the 1.5kg cylindrical type sawdust medium to cultivate the mushroom. The result showed that forest cultivation was the first place cultivated mushroom followed by environmental control system facility and cultivation vinyl house. Among 2 types of sawdust medium, the 1.5kg cylindrical type showed better quantity in terms of a fruit body of mushroom.

This study investigated physiological characteristics and genetic relationship of 30 strains of Lentinula edodes, collected from the Europe, Asia, North America and preserved in the Forest Mushroom Research Institute(FMRI). In physiological characteristics, Papua New Guinea strain was excellent mycelium growth in 25℃ for 7 days on PDA media. For all strains, the optimal temperature for mycelial growth, their tunicate and color of hypha were observed. It surveyed their mycelial growth on oak sawdust media in test tube and independence with inter-strains and cultivar developed in FMRI by strain's anastomosis culture. It was carried by RAPD using operon primers as molecular genetic methods, investigated genetic relationships among strains using UPGMA in NYSYSpc(2.1) according to the presence or absence of bands. <This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries>